LOCUS       pPLaACR26               6783 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 311.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 311
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 29-OCT-2007
            
            
COMMENT     VNTNAME|pPLaACR26|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pPLaACR26" 0 6783 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "EcoRI" "HindIII" "PstI" "XhoI" "BanII") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6783 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 5257 0 6782 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1165 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 2967 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 3 645 0 3671 0 5837 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BanII" "grgcyc" 6 0 5 702 0 3506 0 4012 0 5402 
COMMENT            0 5578 0 0 "")) 
COMMENT       (CObList 
COMMENT        #5=(CFSignal (CObList) "PL promoter" 30 0 1 117 247 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(117..247)") 
COMMENT        #6=(CFSignal (CObList) "KAN" 4 0 0 615 1430 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "615..1430") 
COMMENT        #7=(CFSignal (CObList) "ColE1 ori" 33 0 1 1716 2107 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1716..2107)") 
COMMENT        #8=(CFSignal (CObList) "insertion element 1" 21 0 1 2965 3154 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2965..3154)") 
COMMENT        #9=(CFSignal (CObList) "POL" 4 0 1 3491 5128 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3491..5128)") 
COMMENT        #10=(CFSignal (CObList) "LYS" 4 0 1 4984 5211 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4984..5211)") 
COMMENT        #11=(CFSignal (CObList) "5' UTR of POL" 21 0 1 5129 5161 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5129..5161)") 
COMMENT        #12=(CFSignal (CObList) "5' UTR of COAT" 21 0 1 5555 5577 0 
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COMMENT        #13=(CFSignal (CObList) "5' UTR of A" 21 0 1 6760 6783 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "6783..6760") 
COMMENT        #14=(CFSignal (CObList) "A" 4 0 1 5578 6759 0 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "COAT" 4 0 1 5162 5554 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "LMBP 311" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #17=(CFSignal (CObList) "Last modified: 29-OCT-2007" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pPLaACR26'" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Collection number: LMBP 311." 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/29/07 12:17" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6783 bp" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 6-NOV-2006" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #32=(CRefLinePar 
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COMMENT                                 "" 1))) "LMBP 311") 
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COMMENT                                        "Start: 615   End: 1430" 1) 
COMMENT                                   #42=(CLinePar 
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COMMENT                                        "Tn903 antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #43=(CLinePar 
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COMMENT                                   #47=(CLinePar 
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COMMENT                                        "phage MS2 polymerase coding sequence" 1) 
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COMMENT                                        "   complement(3491..5128)" 1))) "POL") 
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COMMENT                                   #52=(CLinePar 
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COMMENT                                        "phage MS2 lysis protein coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "phage MS2 coat protein coding sequence" 
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COMMENT                                   #62=(CLinePar 
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COMMENT                                        "phage MS2 assembly protein coding sequence" 
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COMMENT                          "CDS (5 total)") 
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COMMENT                                        "insertion element 1" 1) 
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COMMENT                                   #73=(CLinePar 
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COMMENT                                        "5' UTR of the MS2 polymerase gene, incl. RBS" 
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COMMENT                                 "5' UTR of POL") 
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COMMENT                                   #78=(CLinePar 
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COMMENT                                        "5' UTR of the MS2 coat protein gene, incl. RBS" 
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COMMENT                                        "5' UTR of the MS2 assembly protein gene, incl. RBS" 
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COMMENT                                   #85=(CLinePar 
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COMMENT                                        "   6783..6760" 1))) "5' UTR of A"))) 
COMMENT                          "Misc. Feature (4 total)") 
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COMMENT                                   #89=(CLinePar 
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COMMENT                                        "phage lambda major leftward promoter" 1) 
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COMMENT                                        "   complement(117..247)" 1))) 
COMMENT                                 "PL promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                                        "   complement(1716..2107)" 1))) 
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COMMENT                   "Feature Map") 
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FEATURES             Location/Qualifiers
     promoter        complement(117..247)
                     /vntifkey="30"
                     /label=PL\promoter
                     /note="phage lambda major leftward promoter"
     CDS             615..1430
                     /vntifkey="4"
                     /label=KAN
                     /note="Tn903 antibiotic resistance gene; coding sequence"
     rep_origin      complement(1716..2107)
                     /vntifkey="33"
                     /label=ColE1\ori
                     /note="origin plasmid"
     misc_feature    complement(2965..3154)
                     /vntifkey="21"
                     /label=insertion\element\1
                     /note="insertion element 1"
     CDS             complement(3491..5128)
                     /vntifkey="4"
                     /label=POL
                     /note="phage MS2 polymerase coding sequence"
     misc_feature    complement(5129..5161)
                     /vntifkey="21"
                     /label=5'\UTR\of\POL
                     /note="5' UTR of the MS2 polymerase gene, incl. RBS"
     CDS             complement(5162..5554)
                     /vntifkey="4"
                     /label=COAT
                     /note="phage MS2 coat protein coding sequence"
     misc_feature    complement(5555..5577)
                     /vntifkey="21"
                     /label=5'\UTR\of\COAT
                     /note="5' UTR of the MS2 coat protein gene, incl. RBS"
     CDS             complement(4984..5211)
                     /vntifkey="4"
                     /label=LYS
                     /note="phage MS2 lysis protein coding sequence"
     CDS             complement(5578..6759)
                     /vntifkey="4"
                     /label=A
                     /note="phage MS2 assembly protein coding sequence"
     misc_feature    complement(6760..6783)
                     /vntifkey="21"
                     /label=5'\UTR\of\A
                     /note="5' UTR of the MS2 assembly protein gene, incl. RBS"
BASE COUNT     1799 a      1650 c      1669 g      1665 t 
ORIGIN
        1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc 
       61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc 
      121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga 
      181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg 
      241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc 
      301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag 
      361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact 
      421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 
      481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca 
      541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 
      601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa 
      661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 
      721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac 
      781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 
      841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 
      901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt 
      961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 
     1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 
     1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 
     1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc 
     1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 
     1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 
     1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 
     1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 
     1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg 
     1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga 
     1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct 
     1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga 
     1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga 
     1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac 
     1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga 
     1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc 
     1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     2941 tgcgcaacgt tgttgccatt gctgcagttg ccatgtttta cggcagtgag agcagagata 
     3001 gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga 
     3061 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa 
     3121 tcagtaagtt ggcagcatca cccacgatgc ctgcaaaaaa aaaaaaaaaa aaaaaaaaaa 
     3181 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     3241 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     3301 aaaaattttt tttttttttt gggtggtaac tagccaagca gctagttacc aaatcgggag 
     3361 aatcccgggt cctctcttta gggggaggtc cctgggccga agcccgccca cctttcggtg 
     3421 gagccggacc gctttcgcac ccgtgctctt tcgagcacac ccaccccgtt tacgggggtc 
     3481 cctcggtcag ctaccgagga gagctcgctg gcccacactc ctgagggaat gtgggaaccg 
     3541 gcgttagcca ctccgaagtg cgtataacgc gcacgccggc ggacttcatg ctgtcggtga 
     3601 tttcacctcc agtatggaac cacgctatgt agcgaccact gtcgtgcttt tcgctgaaga 
     3661 acttgcgttc tcgagcgata cgagcaagac ggaaacccga ggtacgggta tccgcgagca 
     3721 gccgcccgta cggagtcttg gtgtataccg agactgccgt aggcgggctg actacgtagt 
     3781 agtcggcagc gaggtccgtc ccaccgaaga acatcgaagg cacctgggag gagagccgta 
     3841 cccacacctt atagaggcgt ggatctgaca tacctccgac aactccccaa ccccgtagcc 
     3901 gatttaatat cagcatcagg gcgaagagat tgtcaacagg tttcttgatg taaaacggtt 
     3961 tgacatcgac accacggtaa aagtgcgcgc cgcagctctc gcgaaagagc ccggacacga 
     4021 acgttttacg aagattcggt ttaaaaccgt agtaggcaag tgcctctagc acacggggtg 
     4081 caatctcact gggacatata atatcgtccc cgtagatgcc tatggttccg gcgttaccaa 
     4141 aatggatttg ggtcgctttg actattgccc agaatatcat ggactctagc tcaaatgtga 
     4201 acccatttcc cattgtggaa aatagttccc atcgtatcgt ctcgccatct acgattccgt 
     4261 agtgtgagcg gatacgatcg agatatgaat atagctcagg tgggagaaaa ctccacacca 
     4321 ggcgatcgga gatggaatcg gatgcagacg ataagtctat cgtcgcaagc gaaccatcta 
     4381 cgctgccctg ctgagccaga cgctggttga tcgattgatc attcaggtct ataccaacgg 
     4441 atttgagccg gcgtctgatg aaagcaccga cccctttctg gaggtacata ttcatatcag 
     4501 gctccttaca ggcagcccga tctattttat tattcttcgg aactgtaaac actccgttcc 
     4561 ctacaacgag cctaaattca tatgactcgt tatagcggac cgcgtgtctg atccacggcg 
     4621 cacattggtc tcggaccaat agagccgctc tcagagcgcg gggggtaacg gttgcttgtt 
     4681 cagcgaactt cttgtaaggc gctgcatcct gcaacttgtg ccccatagga gcaccgttgg 
     4741 agaacgtgca ttgcccaaac aacgacgatc ggtagccaga gaggaggttg ccaataaggc 
     4801 tacggatgct ggtttgtaaa acatccggat cccatgacaa ggatttgtca tgtaagaaac 
     4861 cttctctatt tatctgaccg cgatcaccat tcgcctcccg tagcttagcg atagctaagg 
     4921 tacgacgggt cgcctcgtca ttaccagaac ctaaggtcgg atgctttgtg agcaattcgt 
     4981 cccttaagta agcaattgct gtaaagtcgt cactgtgcgg atcaccgctt ccagtagcga 
     5041 cagaagcaat tgattggtaa atttcgagag aaagatcgcg aggaagatca atacataaag 
     5101 agttgaactt ctttgttgtc ttcgacatgg gtaatcctca tgtttgaatg gccggcgtct 
     5161 attagtagat gccggagttt gctgcgattg ctgagggaat cgggtttcca tcttttagga 
     5221 gaccttgcat tgccttaaca ataagctcgc agtcggaatt cgtagcgaaa attggaatgg 
     5281 ttagttccat atttaagtac gaacgccatg cggctacagg aagctctaca ccaccaacag 
     5341 tctgggttgc cactttaggc acctcgactt tgatggtgta tttgcgattc tgcgcagagc 
     5401 tctgacgaac gctacaggtt actttgtaag cctgtgaacg cgagttagag ctgatccatt 
     5461 cagcgacccc gttagcgaag ttgcttgggg cgacagtcac gtcgccagtt ccgccattgt 
     5521 cgacgagaac gaactgagta aagttagaag ccatgcttca aactccggtt gagggctcta 
     5581 tctagagagc cgttgcctga ttaatgctaa cgcatctaag gtatggacca tcgagaaagg 
     5641 agactttacg tacgcgccag ttgttggcca tacggattgt acccctcgat gcatggctga 
     5701 gatttgggcc ttagcagtgc cctgtctctc cacagtccac ccgtagggag cgtcaacgct 
     5761 tatgatggac tcacccgtta ttacgtcagt aactgttcct gacatgtagg agcatcccac 
     5821 gggggccgta aggccctcga gcatgttacc tacaggtagg agccagtcga caacgaatga 
     5881 gaaaggcacc ttttcccaca ctatacctag tgggttcaag atacctagag acgacaacca 
     5941 tgccaaacgt gcatcgttta tgtaaaacca tatcacgata cgtcgcgata tgttgcacgt 
     6001 tgtctggaag tttgcagctg gatacgacag acggccatct aacttgatgt tagtaccgac 
     6061 ctgacgtacg gctctcatag gaagaaactc ttgaaggtga accttcgtaa gcatctcata 
     6121 tgcaccctgg atatcactca ttagtggtaa ccaaccgaac tgcaactcca accacctgcc 
     6181 ggccacgtgt tttgatcgaa actttcgatc ttcgtttagg gcaaggtagc ggagcgcctg 
     6241 gcgccaatta ccgcgacgag cggcagtgta cgccttcacg agcgcaatgg tttgcgtcgc 
     6301 gagttgtgag gctgtcgacc tggcctctgc taaagcaaca ccaaggttaa aattaccctg 
     6361 ggtgaccttt tgcaggactt cggtcgacgc ccggttcgca acgttctgcg gcacttcgat 
     6421 gtaagtcaag ttttggctta cagggaagag gctgtagcag gagcgtgcgt cgagggagaa 
     6481 gccgaaaccg gctttctcct cgtacgggcg accccacgat gacccacttc gcttgtaggc 
     6541 accttgatct atcgatgtga cacttaacgc cccccgtgaa tacggagagg ggtagtgcca 
     6601 ctgtttcgtt ttggccccag tcgagttaaa acgaccggga gtccagttcg aacgatattt 
     6661 taaagagaat gagttatctt cagtctcacc gtccgcgtaa acgcgaacgg aggggacgaa 
     6721 ggtctcgttc tccctatcaa gggtactaaa agctcgcaca ggtcaaacct cctaggaatg 
     6781 gaa 
//
