ID pPLaACR26 standard; circular DNA; ; 6783 BP. DE LMBP 311. CC Accession number: LMBP 311 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter complement(117..247) FT /vntifkey="30" FT /label=PL\promoter FT /note="phage lambda major leftward promoter" FT CDS 615..1430 FT /vntifkey="4" FT /label=KAN FT /note="Tn903 antibiotic resistance gene; coding sequence" FT rep_origin complement(1716..2107) FT /vntifkey="33" FT /label=ColE1\ori FT /note="origin plasmid" FT misc_feature complement(2965..3154) FT /vntifkey="21" FT /label=insertion\element\1 FT /note="insertion element 1" FT CDS complement(3491..5128) FT /vntifkey="4" FT /label=POL FT /note="phage MS2 polymerase coding sequence" FT misc_feature complement(5129..5161) FT /vntifkey="21" FT /label=5'\UTR\of\POL FT /note="5' UTR of the MS2 polymerase gene, incl. RBS" FT CDS complement(5162..5554) FT /vntifkey="4" FT /label=COAT FT /note="phage MS2 coat protein coding sequence" FT misc_feature complement(5555..5577) FT /vntifkey="21" FT /label=5'\UTR\of\COAT FT /note="5' UTR of the MS2 coat protein gene, incl. RBS" FT CDS complement(4984..5211) FT /vntifkey="4" FT /label=LYS FT /note="phage MS2 lysis protein coding sequence" FT CDS complement(5578..6759) FT /vntifkey="4" FT /label=A FT /note="phage MS2 assembly protein coding sequence" FT misc_feature complement(6760..6783) FT /vntifkey="21" FT /label=5'\UTR\of\A FT /note="5' UTR of the MS2 assembly protein gene, incl. RBS" SQ Sequence 6783 BP; 1799 A; 1650 C; 1669 G; 1665 t; ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc 60 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc 120 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga 180 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg 240 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc 300 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag 360 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact 420 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 480 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca 540 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 600 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa 660 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 720 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac 780 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 840 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 900 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt 960 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 1020 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 1080 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 1140 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc 1200 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 1260 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 1320 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 1380 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 1440 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg 1500 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga 1560 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct 1620 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga 1680 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga 1740 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac 1800 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga 1860 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc 1920 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 1980 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 2040 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 2100 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 2160 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 2220 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 2280 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 2340 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 2400 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 2460 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 2520 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 2580 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 2640 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 2700 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 2760 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 2820 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 2880 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 2940 tgcgcaacgt tgttgccatt gctgcagttg ccatgtttta cggcagtgag agcagagata 3000 gcgctgatgt ccggcggtgc ttttgccgtt acgcaccacc ccgtcagtag ctgaacagga 3060 gggacagctg atagaaacag aagccactgg agcacctcaa aaacaccatc atacactaaa 3120 tcagtaagtt ggcagcatca cccacgatgc ctgcaaaaaa aaaaaaaaaa aaaaaaaaaa 3180 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3240 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 3300 aaaaattttt tttttttttt gggtggtaac tagccaagca gctagttacc aaatcgggag 3360 aatcccgggt cctctcttta gggggaggtc cctgggccga agcccgccca cctttcggtg 3420 gagccggacc gctttcgcac ccgtgctctt tcgagcacac ccaccccgtt tacgggggtc 3480 cctcggtcag ctaccgagga gagctcgctg gcccacactc ctgagggaat gtgggaaccg 3540 gcgttagcca ctccgaagtg cgtataacgc gcacgccggc ggacttcatg ctgtcggtga 3600 tttcacctcc agtatggaac cacgctatgt agcgaccact gtcgtgcttt tcgctgaaga 3660 acttgcgttc tcgagcgata cgagcaagac ggaaacccga ggtacgggta tccgcgagca 3720 gccgcccgta cggagtcttg gtgtataccg agactgccgt aggcgggctg actacgtagt 3780 agtcggcagc gaggtccgtc ccaccgaaga acatcgaagg cacctgggag gagagccgta 3840 cccacacctt atagaggcgt ggatctgaca tacctccgac aactccccaa ccccgtagcc 3900 gatttaatat cagcatcagg gcgaagagat tgtcaacagg tttcttgatg taaaacggtt 3960 tgacatcgac accacggtaa aagtgcgcgc cgcagctctc gcgaaagagc ccggacacga 4020 acgttttacg aagattcggt ttaaaaccgt agtaggcaag tgcctctagc acacggggtg 4080 caatctcact gggacatata atatcgtccc cgtagatgcc tatggttccg gcgttaccaa 4140 aatggatttg ggtcgctttg actattgccc agaatatcat ggactctagc tcaaatgtga 4200 acccatttcc cattgtggaa aatagttccc atcgtatcgt ctcgccatct acgattccgt 4260 agtgtgagcg gatacgatcg agatatgaat atagctcagg tgggagaaaa ctccacacca 4320 ggcgatcgga gatggaatcg gatgcagacg ataagtctat cgtcgcaagc gaaccatcta 4380 cgctgccctg ctgagccaga cgctggttga tcgattgatc attcaggtct ataccaacgg 4440 atttgagccg gcgtctgatg aaagcaccga cccctttctg gaggtacata ttcatatcag 4500 gctccttaca ggcagcccga tctattttat tattcttcgg aactgtaaac actccgttcc 4560 ctacaacgag cctaaattca tatgactcgt tatagcggac cgcgtgtctg atccacggcg 4620 cacattggtc tcggaccaat agagccgctc tcagagcgcg gggggtaacg gttgcttgtt 4680 cagcgaactt cttgtaaggc gctgcatcct gcaacttgtg ccccatagga gcaccgttgg 4740 agaacgtgca ttgcccaaac aacgacgatc ggtagccaga gaggaggttg ccaataaggc 4800 tacggatgct ggtttgtaaa acatccggat cccatgacaa ggatttgtca tgtaagaaac 4860 cttctctatt tatctgaccg cgatcaccat tcgcctcccg tagcttagcg atagctaagg 4920 tacgacgggt cgcctcgtca ttaccagaac ctaaggtcgg atgctttgtg agcaattcgt 4980 cccttaagta agcaattgct gtaaagtcgt cactgtgcgg atcaccgctt ccagtagcga 5040 cagaagcaat tgattggtaa atttcgagag aaagatcgcg aggaagatca atacataaag 5100 agttgaactt ctttgttgtc ttcgacatgg gtaatcctca tgtttgaatg gccggcgtct 5160 attagtagat gccggagttt gctgcgattg ctgagggaat cgggtttcca tcttttagga 5220 gaccttgcat tgccttaaca ataagctcgc agtcggaatt cgtagcgaaa attggaatgg 5280 ttagttccat atttaagtac gaacgccatg cggctacagg aagctctaca ccaccaacag 5340 tctgggttgc cactttaggc acctcgactt tgatggtgta tttgcgattc tgcgcagagc 5400 tctgacgaac gctacaggtt actttgtaag cctgtgaacg cgagttagag ctgatccatt 5460 cagcgacccc gttagcgaag ttgcttgggg cgacagtcac gtcgccagtt ccgccattgt 5520 cgacgagaac gaactgagta aagttagaag ccatgcttca aactccggtt gagggctcta 5580 tctagagagc cgttgcctga ttaatgctaa cgcatctaag gtatggacca tcgagaaagg 5640 agactttacg tacgcgccag ttgttggcca tacggattgt acccctcgat gcatggctga 5700 gatttgggcc ttagcagtgc cctgtctctc cacagtccac ccgtagggag cgtcaacgct 5760 tatgatggac tcacccgtta ttacgtcagt aactgttcct gacatgtagg agcatcccac 5820 gggggccgta aggccctcga gcatgttacc tacaggtagg agccagtcga caacgaatga 5880 gaaaggcacc ttttcccaca ctatacctag tgggttcaag atacctagag acgacaacca 5940 tgccaaacgt gcatcgttta tgtaaaacca tatcacgata cgtcgcgata tgttgcacgt 6000 tgtctggaag tttgcagctg gatacgacag acggccatct aacttgatgt tagtaccgac 6060 ctgacgtacg gctctcatag gaagaaactc ttgaaggtga accttcgtaa gcatctcata 6120 tgcaccctgg atatcactca ttagtggtaa ccaaccgaac tgcaactcca accacctgcc 6180 ggccacgtgt tttgatcgaa actttcgatc ttcgtttagg gcaaggtagc ggagcgcctg 6240 gcgccaatta ccgcgacgag cggcagtgta cgccttcacg agcgcaatgg tttgcgtcgc 6300 gagttgtgag gctgtcgacc tggcctctgc taaagcaaca ccaaggttaa aattaccctg 6360 ggtgaccttt tgcaggactt cggtcgacgc ccggttcgca acgttctgcg gcacttcgat 6420 gtaagtcaag ttttggctta cagggaagag gctgtagcag gagcgtgcgt cgagggagaa 6480 gccgaaaccg gctttctcct cgtacgggcg accccacgat gacccacttc gcttgtaggc 6540 accttgatct atcgatgtga cacttaacgc cccccgtgaa tacggagagg ggtagtgcca 6600 ctgtttcgtt ttggccccag tcgagttaaa acgaccggga gtccagttcg aacgatattt 6660 taaagagaat gagttatctt cagtctcacc gtccgcgtaa acgcgaacgg aggggacgaa 6720 ggtctcgttc tccctatcaa gggtactaaa agctcgcaca ggtcaaacct cctaggaatg 6780 gaa 6783 //