LOCUS       pUC13mIL5               4214 bp    DNA     circular     12-DEC-2019
DEFINITION  LMBP 3161.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3161
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 12-DEC-2019
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-13119256|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pUC13mIL5|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CRSite (CStringList) "SspI" "aatatt" 4 0 3 458 0 479 0 4032 0 0 "")) 
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COMMENT        #4=(CFSignal (CObList) "mIL5 signal sequence" 4 0 1 1859 1921 0 
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COMMENT        #5=(CFSignal (CObList) "mIL5 mature sequence" 4 0 1 1520 1858 0 
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COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 1 2036 2119 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 2211 2613 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3154 4014 0 
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COMMENT        #9=(CFSignal (CObList) "R79H" 62 0 1 1685 1687 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
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FEATURES             Location/Qualifiers
     misc_feature    complement(431..1519)
                     /vntifkey="21"
                     /label=3'\UTR\of\mIL5
                     /note="3' UTR of the mouse interleukin 5 gene"
     CDS             complement(1859..1921)
                     /vntifkey="4"
                     /label=mIL5\signal\sequence
                     /note="mouse interleukin 5 signal sequence"
     CDS             complement(1520..1858)
                     /vntifkey="4"
                     /label=mIL5\mature\sequence
                     /note="mouse interleukin 5 mature sequence"
     promoter        complement(2036..2119)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      complement(2211..2613)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(3154..4014)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     mutation        complement(1685..1687)
                     /vntifkey="62"
                     /label=R79H
     RBS             complement(1998..2035)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
BASE COUNT     1077 a      1003 c      1027 g      1107 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cctttttttt tttttttttt 
      421 tttttttttt gagttgtccc ttctgcttta ttaaaatatt atatacgttg taataaatat 
      481 taacttactg aatccatcat aaataatatg aacatcttac aggattaaat aaaagcatac 
      541 atctgttttt cctggagtaa actgggggag gcttctccag tgaccctgat acctgaataa 
      601 catcccaacc attttatgct ttctgttggc atggggtagt aaggttgtgg tatttacttt 
      661 gcttcttaag ttcagttaca tggagaaata aggcccagca tgtggctggc tctcattcac 
      721 actgcaagga gatgtccctt ttcttttcca aaatccagct gctccaggca cagaggcaca 
      781 gtcatggcac agtctgattc atacatagga cataagaaaa gtcaaccttc tctctcccca 
      841 agaaatagaa gtgggcttac tagagtttgg ggagagtagc agggagttga ggagagattg 
      901 actagtgggt attaaattga agttagatag gagcaggaag ccccggtgtg gcatccctca 
      961 gcaagagagt cattaagtat catctatcta aaggggctag gagaaaggat gctaaggttg 
     1021 ggtatgtgat cctcctgcgt ccatctggac caagagttca gggtcaccct tggctacaca 
     1081 ctgagttcaa agtcagcctg gaatacttga gaccctgatg caacgaagag gatgaggggg 
     1141 agggagtata actcagttgg taacatgcac aaagccttgg gttccatctc cagcacttca 
     1201 tgaactagtg gcaatggtgc atgtctgtaa cctcagcctt caggaagagg aggcaggagg 
     1261 atcagaaatt caaggttatc cttggctaca ttaccagttt gaggccagcc tgcgctacag 
     1321 gaatttcttt tatgcgtata caaaaaggtt aaacactttg catatatgga catagatagg 
     1381 tttaactgac tacaggacat aaatataaat ggaaacagca tatggtatcc cttgcatttg 
     1441 cacagttttg tggggttttt gcatctgttg acaatttaac ttaaattgtg aagtcctgtc 
     1501 accatggagc agctcagcct cagccttcca ttgcccactc cgtactcatc acaccaagga 
     1561 actcttgcag gtaatccagg aactgcctcg tcctccgtct ctcctcgcca cacttctctt 
     1621 tttggcggtc aatgtatttc tttattaatg acaggttttg gaatagcatt tccacagtac 
     1681 ccccatggac agtttgattc ttcagtatgt ctagcccctg aaagatttct ccaatgcata 
     1741 gctggtgatt tttatgagta gggacaggaa gcctcatcgt ctcattgctt gtcaacagag 
     1801 ctcggtgagc agacagctgt gtcaaggtct ctttcaccac tgtgctcatg ggaatctcca 
     1861 tggcagtggc ccagacacag ctgagagtca gaacactcaa gtgcagaagc atccttctca 
     1921 tgactctggg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt 
     1981 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca 
     2041 caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag tgagctaact 
     2101 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct 
     2161 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc 
     2221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca 
     2281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg 
     2341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca 
     2401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa 
     2461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc 
     2521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc 
     2581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct 
     2641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg 
     2701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag 
     2761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta 
     2821 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg 
     2881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt 
     2941 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt 
     3001 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag 
     3061 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat 
     3121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc 
     3181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat 
     3241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc 
     3301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag 
     3361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag 
     3421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt 
     3481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg 
     3541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt 
     3601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc 
     3661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc 
     3721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa 
     3781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg 
     3841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc 
     3901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag 
     3961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt 
     4021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt 
     4081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc 
     4141 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac 
     4201 gaggcccttt cgtc 
//
