LOCUS       pJBtrc                  7952 bp    DNA     circular      8-OCT-2008
DEFINITION  LMBP 3177.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3177
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 8-OCT-2008
            
COMMENT     VNTNAME|pJBtrc|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 6761 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SphI" "gcatgc" 6 0 3 1507 0 5286 0 6924 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 7070 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 1 3493 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 6936 0 0 "")) 
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COMMENT        #8=(CFSignal (CObList) "trfA" 4 0 1 2696 3844 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "Tn5 neomycin resistance promoter" 30 0 1 3953 
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COMMENT        #10=(CFSignal (CObList) "RK2 ori" 33 0 1 4242 4766 0 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "lacIq promoter" 30 0 0 5296 5373 0 
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COMMENT        #13=(CFSignal (CObList) "trc promoter" 30 0 0 6679 6722 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 6723 6758 0 
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COMMENT                          "DNA Plasmid 'pJBtrc'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(793..1983)
                     /vntifkey="4"
                     /label=tetracycline\resistance
                     /note="antibiotic resistance gene; coding sequence"
     CDS             complement(2696..3844)
                     /vntifkey="4"
                     /label=trfA
                     /note="P382 coding sequence; P285 starts at 3553"
     promoter        complement(3953..4002)
                     /vntifkey="30"
                     /label=Tn5\neomycin\resistance\promoter
     rep_origin      complement(4242..4766)
                     /vntifkey="33"
                     /label=RK2\ori
     promoter        5296..5373
                     /vntifkey="30"
                     /label=lacIq\promoter
                     /note="mutated E. coli repressor lacI promoter, incl. RBS"
     CDS             5374..6456
                     /vntifkey="4"
                     /label=lacI
                     /note="E. coli repressor lacI coding sequence"
     promoter        6679..6722
                     /vntifkey="30"
                     /label=trc\promoter
     RBS             6723..6758
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
                     /note="5' UTR of the E. coli lacZ gene, incl. RBS"
     rep_origin      complement(7665..7775)
                     /vntifkey="33"
                     /label=RK2/RP4\oriT
BASE COUNT     1753 a      2204 c      2192 g      1803 t 
ORIGIN
        1 tcctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat 
       61 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg 
      121 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc 
      181 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg 
      241 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc 
      301 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag 
      361 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg 
      421 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg 
      481 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg 
      541 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga 
      601 gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc 
      661 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag 
      721 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc 
      781 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag 
      841 gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga 
      901 ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc 
      961 cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc 
     1021 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat 
     1081 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc 
     1141 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc 
     1201 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg 
     1261 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat 
     1321 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga 
     1381 gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg 
     1441 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg 
     1501 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac 
     1561 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc 
     1621 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc 
     1681 ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc 
     1741 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac 
     1801 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc 
     1861 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc 
     1921 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt 
     1981 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt 
     2041 atcgatgata agctgtcaaa catgagaatt cactggccgt cgttttacaa cgtcgtgact 
     2101 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 
     2161 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 
     2221 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 
     2281 tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca 
     2341 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 
     2401 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 
     2461 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 
     2521 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 
     2581 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 
     2641 gcttcaataa tcatgcaggt gctgaacccc cagccggaac tgaccccaca aggccctagc 
     2701 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca 
     2761 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc 
     2821 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat 
     2881 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg 
     2941 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg 
     3001 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt 
     3061 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct 
     3121 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga 
     3181 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc 
     3241 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct 
     3301 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg 
     3361 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg 
     3421 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca 
     3481 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct 
     3541 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct 
     3601 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag 
     3661 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg 
     3721 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct 
     3781 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat 
     3841 tcattcaccc tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct 
     3901 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg 
     3961 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa 
     4021 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc 
     4081 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg 
     4141 tttctgcgga ctggctttct acgtggctgc catttttggg gtgaggtcgt tcgcggccga 
     4201 ggggcgcagc ccctgggggg atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg 
     4261 gggggcaccc cccttcggcg tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca 
     4321 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac 
     4381 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat 
     4441 aggtgcgccc ctcatctgtc atcactctgc ccctcaagtg tcaaggatcg cgcccctcat 
     4501 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc 
     4561 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc 
     4621 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt 
     4681 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc 
     4741 gcgtggtatc cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct 
     4801 ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     4861 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     4921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     4981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 
     5041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 
     5101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gttgtctgct 
     5161 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 
     5221 ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg 
     5281 gcatgcattt acgttgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag 
     5341 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc 
     5401 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac 
     5461 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc 
     5521 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc 
     5581 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa 
     5641 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg 
     5701 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat 
     5761 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat 
     5821 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg 
     5881 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta 
     5941 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa 
     6001 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc 
     6061 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg 
     6121 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg 
     6181 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtc aaccaccatc 
     6241 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag 
     6301 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc 
     6361 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 
     6421 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 
     6481 gcgcgaattg atctggtttg acagcttatc atcgactgca cggtgcacca atgcttctgg 
     6541 cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt 
     6601 cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg 
     6661 ttctggcaaa tattctgaaa tgagctgttg acaattaatc atccggctcg tataatgtgt 
     6721 ggaattgtga gcggataaca atttcacaca ggaaacagac catggctggt gaccacgtcg 
     6781 tggaatgcct tcgaattcag cacctgcaca tgggacgtcg acctgaggta attataaccc 
     6841 gggccctata tatggatcca attgcaatga tcatcatgac agatctgcgc gcgatcgata 
     6901 tcagcgcttt aaatttgcgc atgctagcta tagttctaga ggtaccggtt gttaacgtta 
     6961 gccggctacg tatactccgg aatattaata ggcctaggat gcatatggcg gccgcctgca 
     7021 gctggcgcca tcgatacgcg tacgtcgcga ccgcggacat gtacagagct cgagaagtac 
     7081 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 
     7141 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 
     7201 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 
     7261 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 
     7321 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 
     7381 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     7441 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     7501 cgaaaagtgc cacctgggtc gatcgacgga tcttttccgc tgcataaccc tgcttcgggg 
     7561 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc 
     7621 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg 
     7681 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg 
     7741 tgctcaacgg gaatcctgct ctgcgaggct ggccgtaagc tctaagaaac cattattatc 
     7801 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattaatt 
     7861 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 
     7921 gccttgcagc acatccccct ttcgccagca ga 
//
