ID pJBtrc standard; circular DNA; ; 7952 BP. DE LMBP 3177. CC Accession number: LMBP 3177 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 8-OCT-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(793..1983) FT /vntifkey="4" FT /label=tetracycline\resistance FT /note="antibiotic resistance gene; coding sequence" FT CDS complement(2696..3844) FT /vntifkey="4" FT /label=trfA FT /note="P382 coding sequence; P285 starts at 3553" FT promoter complement(3953..4002) FT /vntifkey="30" FT /label=Tn5\neomycin\resistance\promoter FT rep_origin complement(4242..4766) FT /vntifkey="33" FT /label=RK2\ori FT promoter 5296..5373 FT /vntifkey="30" FT /label=lacIq\promoter FT /note="mutated E. coli repressor lacI promoter, incl. RBS" FT CDS 5374..6456 FT /vntifkey="4" FT /label=lacI FT /note="E. coli repressor lacI coding sequence" FT promoter 6679..6722 FT /vntifkey="30" FT /label=trc\promoter FT RBS 6723..6758 FT /vntifkey="32" FT /label=5'\UTR\of\lacZ,\incl.\RBS FT /note="5' UTR of the E. coli lacZ gene, incl. RBS" FT rep_origin complement(7665..7775) FT /vntifkey="33" FT /label=RK2/RP4\oriT SQ Sequence 7952 BP; 1753 A; 2204 C; 2192 G; 1803 t; tcctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat 60 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg 120 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc 180 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg 240 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc 300 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag 360 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg 420 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg 480 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg 540 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga 600 gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc 660 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag 720 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc 780 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag 840 gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga 900 ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc 960 cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc 1020 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat 1080 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc 1140 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc 1200 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg 1260 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat 1320 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga 1380 gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg 1440 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg 1500 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac 1560 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc 1620 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc 1680 ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc 1740 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac 1800 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc 1860 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc 1920 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt 1980 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt 2040 atcgatgata agctgtcaaa catgagaatt cactggccgt cgttttacaa cgtcgtgact 2100 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 2160 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 2220 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 2280 tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca 2340 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 2400 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 2460 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 2520 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 2580 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 2640 gcttcaataa tcatgcaggt gctgaacccc cagccggaac tgaccccaca aggccctagc 2700 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca 2760 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc 2820 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat 2880 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg 2940 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg 3000 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt 3060 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct 3120 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga 3180 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc 3240 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct 3300 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg 3360 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg 3420 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca 3480 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct 3540 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct 3600 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag 3660 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg 3720 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct 3780 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat 3840 tcattcaccc tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct 3900 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg 3960 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa 4020 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc 4080 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg 4140 tttctgcgga ctggctttct acgtggctgc catttttggg gtgaggtcgt tcgcggccga 4200 ggggcgcagc ccctgggggg atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg 4260 gggggcaccc cccttcggcg tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca 4320 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac 4380 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat 4440 aggtgcgccc ctcatctgtc atcactctgc ccctcaagtg tcaaggatcg cgcccctcat 4500 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc 4560 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc 4620 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt 4680 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc 4740 gcgtggtatc cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct 4800 ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt 4860 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 4920 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 4980 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 5040 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 5100 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gttgtctgct 5160 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt 5220 ttcaccgtca tcaccgaaac gcgcgaggca gcagatcaat tcgcgcgcga aggcgaagcg 5280 gcatgcattt acgttgacac catcgaatgg tgcaaaacct ttcgcggtat ggcatgatag 5340 cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt atacgatgtc 5400 gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca ggccagccac 5460 gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa ttacattccc 5520 aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt tgccacctcc 5580 agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg cgccgatcaa 5640 ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc ctgtaaagcg 5700 gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta tccgctggat 5760 gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt atttcttgat 5820 gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg tacgcgactg 5880 ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc gggcccatta 5940 agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac tcgcaatcaa 6000 attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt tcaacaaacc 6060 atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa cgatcagatg 6120 gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc ggatatctcg 6180 gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtc aaccaccatc 6240 aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca actctctcag 6300 ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag aaaaaccacc 6360 ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg 6420 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta 6480 gcgcgaattg atctggtttg acagcttatc atcgactgca cggtgcacca atgcttctgg 6540 cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca ctgcataatt 6600 cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac atcataacgg 6660 ttctggcaaa tattctgaaa tgagctgttg acaattaatc atccggctcg tataatgtgt 6720 ggaattgtga gcggataaca atttcacaca ggaaacagac catggctggt gaccacgtcg 6780 tggaatgcct tcgaattcag cacctgcaca tgggacgtcg acctgaggta attataaccc 6840 gggccctata tatggatcca attgcaatga tcatcatgac agatctgcgc gcgatcgata 6900 tcagcgcttt aaatttgcgc atgctagcta tagttctaga ggtaccggtt gttaacgtta 6960 gccggctacg tatactccgg aatattaata ggcctaggat gcatatggcg gccgcctgca 7020 gctggcgcca tcgatacgcg tacgtcgcga ccgcggacat gtacagagct cgagaagtac 7080 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca 7140 acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt 7200 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc 7260 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca 7320 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata 7380 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 7440 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 7500 cgaaaagtgc cacctgggtc gatcgacgga tcttttccgc tgcataaccc tgcttcgggg 7560 tcattatagc gattttttcg gtatatccat cctttttcgc acgatataca ggattttgcc 7620 aaagggttcg tgtagacttt ccttggtgta tccaacggcg tcagccgggc aggataggtg 7680 aagtaggccc acccgcgagc gggtgttcct tcttcactgt cccttattcg cacctggcgg 7740 tgctcaacgg gaatcctgct ctgcgaggct ggccgtaagc tctaagaaac cattattatc 7800 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaattaatt 7860 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc 7920 gccttgcagc acatccccct ttcgccagca ga 7952 //