LOCUS       pLOF/Km                10146 bp    DNA     circular      8-JUL-2010
DEFINITION  LMBP 3337.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 3337
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 8-JUL-2010
            
            
COMMENT     VNTDATE|495737853|
COMMENT     VNTNAME|pLOF/Km|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pLOF/Km" 0 10146 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 2 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "BglII" "EcoRI" "EcoRV" "MluI" "NotI" "PstI" "SacI" 
COMMENT         "SphI" "SfiI" "Bsp68I") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "Tn903 neomycin resistance" 
COMMENT             "ATGAGCCATATTCAACGGGAAACGTCTTGCTCGAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGGAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGCTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA" 
COMMENT             "Fragment of Accession number V00359; Length: 816 bp " 0 
COMMENT             (CStringList) 0)) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 
COMMENT        "MAIN" 0 0 30 0 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1 0 4826 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 3931 0 6095 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "MluI" "acgcgt" 2 0 3 4377 0 7515 0 9070 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 2 7522 0 9050 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PstI" "ctgcag" 6 0 3 2962 0 3637 0 3645 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 4818 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "SphI" "gcatgc" 6 0 1 5382 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SfiI" "ggccnnnnnggcc" 9 0 1 9064 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "Bsp68I" "tcgcga" 4 0 2 5112 0 8585 0 0 "")) 
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COMMENT        #13=(CFSignal (CObList) "R6K ori" 33 0 1 63 441 0 (CStringList) 
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COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2643 3503 0 
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COMMENT        #15=(CFSignal (CObList) "lacI" 4 0 1 3649 4731 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3649..4731)") 
COMMENT        #16=(CFSignal (CObList) "lacIq promoter" 30 0 1 4732 4809 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4732..4809)") 
COMMENT        #17=(CFSignal (CObList) "IS10 IR" 21 0 0 9172 9241 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "9172..9241") 
COMMENT        #18=(CFSignal (CObList) "IS10 IR" 21 0 0 7347 7416 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7347..7416") 
COMMENT        #19=(CFSignal (CObList) "IS10R" 4 0 0 5138 6346 0 (CStringList) 
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COMMENT        #20=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 5061 5096 0 
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COMMENT        #21=(CFSignal (CObList) "tac promoter" 30 0 0 5018 5060 0 (CStringList) 
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COMMENT        #22=(CFSignal (CObList) "Tn10 neomycin resistance" 4 0 1 7858 8673 0 
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COMMENT                          "DNA Plasmid 'pLOF/Km'" 1) 
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COMMENT                          "Created: 10/09/08 04:57" 1) 
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COMMENT                          "Last Modified: 07/08/10 01:53" 1) 
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COMMENT                          "length: 10146 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 15-OCT-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "mutated E. coli repressor lacI promoter, incl. RBS" 
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FEATURES             Location/Qualifiers
     rep_origin      complement(1062..1172)
                     /vntifkey="33"
                     /label=RK2/RP4\oriT
     rep_origin      complement(63..441)
                     /vntifkey="33"
                     /label=R6K\ori
     CDS             complement(2643..3503)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             complement(3649..4731)
                     /vntifkey="4"
                     /label=lacI
     promoter        complement(4732..4809)
                     /vntifkey="30"
                     /label=lacIq\promoter
                     /note="mutated E. coli repressor lacI promoter, incl. RBS"
     misc_feature    9172..9241
                     /vntifkey="21"
                     /label=IS10\IR
                     /note="IS10 inverted repeat"
     misc_feature    7347..7416
                     /vntifkey="21"
                     /label=IS10\IR
                     /note="IS10 inverted repeat"
     CDS             5138..6346
                     /vntifkey="4"
                     /label=IS10R
                     /note="coding sequence of the IS10R (insertion sequence 10 right) transposase gene"
     CDS             complement(7858..8673)
                     /vntifkey="4"
                     /label=Tn10\neomycin\resistance
     RBS             5061..5096
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        5018..5060
                     /vntifkey="30"
                     /label=tac\promoter
BASE COUNT     2677 a      2532 c      2391 g      2540 t         6 others
ORIGIN
        1 aattcgatcg ctagtttgtt ttgactccat ccattagggc ttctaaaacg ccttctaagg 
       61 ccatgtcagc cgttaagtgt tcctgtgtca ctgaaaattg ctttgagagg ctctaagggc 
      121 ttctcagtgc gttacatccc tggcttgttg tccacaaccg ttaaacctta aaagctttaa 
      181 aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct atttaagttg 
      241 ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa catgagagct 
      301 tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc gttaaacatg 
      361 agagcttagt acgttaaaca tgagagctta gtacgtgaaa catgagagct tagtacgtac 
      421 tatcaacagg ttgaactgct ggatcctttt tgtccggtgt tgggttgaag gtgaagccgg 
      481 tcggggccgc agcgggggcc ggcttttcag ccttgccccc ctgcttcggc cgccgtggct 
      541 ccggcgtctt gggtgccggc gcgggttccg cagccttggc ctgcggtgcg ggcacatcgg 
      601 cgggcttggc cttgatgtgc cgcctggcgt gcgagcggaa cgtctcgtag gagaacttga 
      661 ccttccccgt ttcccgcatg tgctcccaaa tggtgacgag cgcatagccg gacgctaacg 
      721 ccgcctcgac atccgccctc accgccagga acgcaaccgc agcctcatca cgccggcgct 
      781 tcttggccgc gcgggattca acccactcgg ccagctcgtc ggtgtagctc tttggcatcg 
      841 tctctcgcct gtcccctcag ttcagtaatt tcctgcattt gcctgtttcc agtcggtaga 
      901 tattccacaa aacagcaggg aagcagcgct tttccgctgc ataaccctgc ttcggggtca 
      961 ttatagcgat tttttcggta tatccatcct ttttcgcacg atatacagga ttttgccaaa 
     1021 gggttcgtgt agactttcct tggtgtatcc aacggcgtca gccgggcagg ataggtgaag 
     1081 taggcccacc cgcgagcggg tgttccttct tcactgtccc ttattcgcac ctggcggtgc 
     1141 tcaacgggaa tcctgctctg cgaggctggc cggctaccgc cggcgtaaca gatgagggca 
     1201 agcggatggc tgatgaaacc aagccaacca ggaagggcag cccacctatc aaggtgtact 
     1261 gccttccaga cgaacgaaga gcgattgagg aaaaggcggc ggcggccggc atgagcctgt 
     1321 cggcctacct gctggccgtc ggccagggct acaaaatcac gggcgtcgtg gactatgagc 
     1381 acgtccgcga gctggcccgc atcaatggcg acctgggccg cctgggcggc ctgctgaaac 
     1441 tctggctcac cgacgacccg cgcacggcgc ggttcggtga tgccacgatc ctcgccctgc 
     1501 tggcgaagat cgaagagaag caggacgagc ttggcaaggt catgatgggc gtggtccgcc 
     1561 cgagggcaga gccatgactt ttttagccgc taaaacggcc ggggggtgcg cgtgattgcc 
     1621 aagcacgtcc ccatgcgctc catcaagaag agcgacttcg cggagctggt gaagtacatc 
     1681 accgacgagc aaggcaagac cgagcgcctg ggtcacgtgc gcgtcacgaa ctgcgaggca 
     1741 aacaccctgc ccgctgtcat ggccgaggtg atggcgaccc agcacggcaa cacccgttcc 
     1801 gaggccgaca agacctatca cctgctggtt agcttccgcg cgggagagaa gcccgacgcg 
     1861 gagacgttgc gcgcgattga ggaccgcatc tgcgctgggc ttggcttcgc cgagcatcag 
     1921 cgcgtcagtg ccgtgcatca cgacaccgac aacctgcaca tccatatcgc catcaacaag 
     1981 attcacccga cccgaaacac catccatgag ccgtatcggg cctaccgcgc cctcgctgac 
     2041 ctctgcgcga cgctcgaacg ggactacggg cttgagcgtg acaatcacga aacgcggcag 
     2101 cgcgtttccg agaaccgcgc gaacgacatg gagcggcacg cgggcgtgga aagcctggtc 
     2161 ggctggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt 
     2221 gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc 
     2281 atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc 
     2341 gccatctcct tgctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca 
     2401 gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca 
     2461 gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga 
     2521 tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact gagagtgcac 
     2581 caaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     2641 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     2701 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     2761 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     2821 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     2881 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     2941 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     3001 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     3061 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     3121 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     3181 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     3241 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 
     3301 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     3361 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     3421 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     3481 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     3541 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     3601 gttccgcgca catttccccg aaaagtgcca cctgcagatc tgcagnnntc actgcccgct 
     3661 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 
     3721 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag 
     3781 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg 
     3841 ccccagcagg cgaaaatcct gtttgatggt ggttgacggc gggatataac atgagctgtc 
     3901 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt 
     3961 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac 
     4021 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc 
     4081 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag 
     4141 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc 
     4201 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact 
     4261 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc 
     4321 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg 
     4381 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat 
     4441 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg 
     4501 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc 
     4561 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac 
     4621 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg 
     4681 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac 
     4741 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcaccattc 
     4801 gatggtgtcn nngagctctc ccgggaattc tcatgtttga cagcttatca tcgactgcac 
     4861 ggtgcaccaa tgcttctggc gtcaggcagc catcggaagc tgtggtatgg ctgtgcaggt 
     4921 cgtaaatcac tgcataattc gtgtcgctca aggcgcactc ccgttctgga taatgttttt 
     4981 tgcgccgaca tcataacggt tctggcaaat attctgaaat gagctgttga caattaatca 
     5041 tcggctcgta taatgtgtgg aattgtgagc ggataacaat ttcacacagg aaacaggatc 
     5101 aaatggtttc gcgaaaaatc aataatcaga caacaagatg tgcgaactcg atattttaca 
     5161 cgactctctt taccaattct gccccgaatt acacttaaaa cgactcaaca gcttaacgtt 
     5221 ggcttgccac gcattacttg actgtaaaac tctcactctt accgaacttg gccgtaacct 
     5281 gccaaccaaa gcgagaacaa aacataacat caaacgaatc gaccgattgt taggtaatcg 
     5341 tcacctccac aaagagcgac tcgctgtata ccgttggcat gctagcttta tctgttcggg 
     5401 caatacgatg cccattgtac ttgttgactg gtctgatatt cgtgagcaaa aacgacttat 
     5461 ggtattgcga gcttcagtcg cactacacgg tcgttctgtt actctttatg agaaagcgtt 
     5521 cccgctttca gagcaatgtt caaagaaagc tcatgaccaa tttctagccg accttgcgag 
     5581 cattctaccg agtaacacca caccgctcat tgtcagtgat gctggcttta aagtgccatg 
     5641 gtataaatcc gttgagaagc tgggttggta ctggttaagt cgagtaagag gaaaagtaca 
     5701 atatgcagac ctaggagcgg aaaactggaa acctatcagc aacttacatg atatgtcatc 
     5761 tagtcactca aagactttag gctataagag gctgactaaa agcaatccaa tctcatgcca 
     5821 aattctattg tataaatctc gctctaaagg ccgaaaaaat cagcgctcga cacggactca 
     5881 ttgtcaccac ccgtcaccta aaatctactc agcgtcggca aaggagccat gggttctagc 
     5941 aactaactta cctgttgaaa ttcgaacacc caaacaactt gttaatatct attcgaagcg 
     6001 aatgcagatt gaagaaacct tccgagactt gaaaagtcct gcctacggac taggcctacg 
     6061 ccatagccga acgagcagct cagagcgttt tgatatcatg ctgctaatcg ccctgatgct 
     6121 tcaactaaca tgttggcttg cgggcgttca tgctcagaaa caaggttggg acaagcactt 
     6181 ccaggctaac acagtcagaa atcgaaacgt actctcaaca gttcgcttag gcatggaagt 
     6241 tttgcggcat tctggctaca caataacaag ggaagactta ctcgtggctg caaccctact 
     6301 agctcaaaat ttattcacac atggttacgc tttggggaaa ttatgagggg atctctcagt 
     6361 gcattgcctc caattcccat aatttattac gccgataata acttggtgta accttaaaaa 
     6421 tgtacttaaa tcgacgtgta aaagattgtt gggaatcaaa ttgatatttt aatgcgatct 
     6481 caaggatagt ttttttcgtc aacctcaact caacagccgc tttcgtcaaa cgacgagcac 
     6541 gaatatagct agccagtgtg acccctgtta ctttttgaac agccgctgaa aataccactt 
     6601 ggtataaccc gctttattcg ccacatcatc aagcagtaaa gactgatcta aattatgttc 
     6661 aatccataat agaacatctt tgataaccgt tgtatgaaac tgcttatcat catatcttaa 
     6721 ttggatgtta ttagctttat tttgatagcg agaatgctgt tcaatataca taaaataacc 
     6781 taaatgttct taagattgtc acgaccacat catcatgata ccataaacat actgacggta 
     6841 tgttatttta aatctatcat ggaaaataaa aatcatcaac aagaaaattt taagagtacc 
     6901 tatcaatcac tggttaactc agcacgaata ttgtttgttg aaaaaggcta tcaagctgtt 
     6961 tcaatagatg agatcgatct cgggaaaagc gttggtgacc aaaggtgcct tttatcatca 
     7021 ctttaaaaat aaaaaacaat tactcagtgc ctgttataag cagcaattaa ttatgattga 
     7081 tgcctacatc acaacaaaaa ctgatttaac aaatggttgg tctgccttag aaagtatatt 
     7141 tgaacattat cttgattata ttattgataa taataaaaac cttatcccta tccaagaagt 
     7201 gatgcctatc attggttgga atgaacttga aaaaattagc cttgaataca ttactggtaa 
     7261 ggtaaacgcc attgtcagca aattgatcca agagaaccaa cttaaagctt tcctgacgga 
     7321 atgttaattc tcgttgaccc tgagcactga tgaatcccct aatgattttg gtaaaaatca 
     7381 ttaagttaag gtggatacac atcttgtcat atgatcccgg atccggctgt aatccgggca 
     7441 gcgcaacgga acattcatca gtgtaaaaat ggaatcaata aagccctgcg cagcgcgcag 
     7501 ggtcagcctg aatacgcgtg cggccgccgc gtttaatgac cagcacagtc gtgatggcaa 
     7561 ggtcagaata gcgctgaggt ctgcctcgtg aagaaggtgt tgctgactca taccaggcct 
     7621 gaatcgcccc atcatccagc cagaaagtga gggagccacg gttgatgaga gctttgttgt 
     7681 aggtggacca gttggtgatt ttgaactttt gctttgccac ggaacggtct gcgttgtcgg 
     7741 gaagatgcgt gatctgatcc ttcaactcag caaaagttcg atttattcaa caaagccgcc 
     7801 gtcccgtcaa gtcagcgtaa tgctctgcca gtgttacaac caattaacca attctgatta 
     7861 gaaaaactca tcgagcatca aatgaaactg caatttattc atatcaggat tatcaatacc 
     7921 atatttttga aaaagccgtt tctgtaatga aggagaaaac tcaccgaggc agttccatag 
     7981 gatggcaaga tcctggtatc ggtctgcgat tccgactcgt ccaacatcaa tacaacctat 
     8041 taatttcccc tcgtcaaaaa taaggttatc aagtgagaaa tcaccatgag tgacgactga 
     8101 atccggtgag aatggcaaaa gcttatgcat ttctttccag acttgttcaa caggccagcc 
     8161 attacgctcg tcatcaaaat cactcgcatc aaccaaaccg ttattcattc gtgattgcgc 
     8221 ctgagcgaga cgaaatacgc gatcgctgtt aaaaggacaa ttacaaacag gaatcgaatg 
     8281 caaccggcgc aggaacactg ccagcgcatc aacaatattt tcacctgaat caggatattc 
     8341 ttctaatacc tggaatgctg ttttcccggg gatcgcagtg gtgagtaacc atgcatcatc 
     8401 aggagtacgg ataaaatgct tgatggtcgg aagaggcata aattccgtca gccagtttag 
     8461 tctgaccatc tcatctgtaa catcattggc aacgctacct ttgccatgtt tcagaaacaa 
     8521 ctctggcgca tcgggcttcc catacaatcg atagattgtc gcacctgatt gcccgacatt 
     8581 atcgcgagcc catttatacc catataaatc agcatccatg ttggaattta atcgcggcct 
     8641 cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac tgtttatgta 
     8701 agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt aacatcagag 
     8761 attttgagac acaacgtggc tttgttgaat aaatcgaact tttgctgagt tgaaggatca 
     8821 gatcacgcat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac 
     8881 caactggtcc acctacaaca aagctctcat caaccgtggc tccctcactt tctggctgga 
     8941 tgatggggcg attcaggcct ggtatgagtc agcaacacct tcttcacgag gcagacctca 
     9001 gcgctattct gaccttgcca tcacgactgt gctggtcatt aaacgcggcg gccgcggcct 
     9061 aggcggccac gcgtattcag gctgaccctg cgcgctgcgc agggctttat tgattccatt 
     9121 tttacactga tgaatgttcc gttgcgctgc ccggattaca gccggatccg ggatcatatg 
     9181 acaagatgtg tatccacctt aacttaatga tttttaccaa aatcattagg ggattcatca 
     9241 gtgctcaggg tcaacgagaa ttaacattcc gtcaggaaag cttatgatga tgatgtgctt 
     9301 aaaaacttac tcaatggctg gtttatgcat atcgcaatac atgcgaaaaa cctaaaagag 
     9361 cttgccgata aaaaaggcca atttattgct atttaccgcg gctttttatt gagcttgaaa 
     9421 gataaataaa atagataggt tttatttgaa gctaaatctt ctttatcgta aaaaatgccc 
     9481 tcttgggtta tcaagagggt cattatattt cgcggaataa catcatttgg tgacgaaata 
     9541 actaagcact tgtctcctgt ttactcccct gagcttgagg ggttaacatg aaggtcatcg 
     9601 atagcaggat aataatacag taaaacgcta aaccaataat ccaaatccag ccatcccaaa 
     9661 ttggtagtga atgattataa ataacagcaa acagtaatgg gccaataaca ccggttgcat 
     9721 tggtaaggct caccaataat ccctgtaaag caccttgctg atgactcttt gtttggatag 
     9781 acatcactcc ctgtaatgca ggtaaagcga tcccaccacc agccaataaa attaaaacag 
     9841 ggaaaactaa ccaaccttca gatataaacg ctaaaaaggc aaatgcacta ctatctgcaa 
     9901 taaatccgag cagtactgcc gttttttcgc cccatttagt ggctattctt cctgccacaa 
     9961 aggcttggaa tactgagtgt aaaagaccaa gacccgctaa tgaaaagcca accatcatgc 
    10021 tattccatcc aaaacgattt tcggtaaata gcacccacac cgttgcggga atttggccta 
    10081 tcaattgcgc tgaaaaataa ataatcaaca aaatgggcat cgttttaaat aaagtgatgt 
    10141 ataccg 
//
