ID pLT10TH standard; circular DNA; ; 3653 BP. DE LMBP 3385. CC Accession number: LMBP 3385 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 3-JUN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 24..40 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT RBS 41..103 FT /vntifkey="32" FT /label=5'\UTR\of\T7\gene\10,\incl.\RBS FT /note="5' UTR of phage T7 gene 10, incl. RBS" FT promoter complement(248..264) FT /vntifkey="30" FT /label=T3\promoter FT /note="phage T3 promoter" FT terminator 282..329 FT /vntifkey="43" FT /label=T7\terminator FT /note="phage T7 gene 10 terminator" FT terminator 403..450 FT /vntifkey="43" FT /label=T7\terminator FT /note="phage T7 gene 10 terminator" FT terminator 519..600 FT /vntifkey="43" FT /label=fd\terminator FT /note="phage fd terminator" FT terminator 629..710 FT /vntifkey="43" FT /label=fd\terminator FT /note="phage fd terminator" FT rep_origin 808..1263 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT rep_origin complement(1267..1640) FT /vntifkey="33" FT /label=pMB1\ori FT /note="E. coli plasmid pMB1 origin" FT CDS complement(2181..3041) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT promoter 3407..3537 FT /vntifkey="30" FT /label=PL\promoter FT /note="phage lambda major leftward promoter" FT misc_marker 113..130 FT /vntifkey="22" FT /label=His-tag FT misc_marker 131..145 FT /vntifkey="22" FT /label=EK\recognition\site FT /note="enterokinase recognition site" SQ Sequence 3653 BP; 949 A; 900 C; 858 G; 946 t; ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 60 ctctagaaat aattttgttt aactttaaga aggagatata catatggatc cacatcacca 120 tcaccatcac gacgatgacg ataaggcgcc cgggttcgaa atcgataagc ttatgcatgc 180 ggccgcatct agagggcccg gatccctcga ggtcgacgaa ttcgagctcg gccgacttgg 240 ccttcccttt agtgagggtt aataaacttg gtgagcaata actagcataa ccccttgggg 300 cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatgc gctcatacga 360 tatgaacgtt gagactgccg ctgagttatc agtgagcaat aactagcata accccttggg 420 gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatc cggatagctt 480 atcgctagag gtcgaaattc acctcgaaag caagctgata aaccgataca attaaaggct 540 ccttttggag cctttttttt tggagatttt caacgtgaaa aaattattat tcgcaattcc 600 aagctaattc acctcgaaag caagctgata aaccgataca attaaaggct ccttttggag 660 cctttttttt tggagatttt caacgtgaaa aaattattat tcgcaattcc aagctctgcc 720 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 780 cagcttgtct gtaagcggat gcagatcacg cgccctgtag cggcgcatta agcgcggcgg 840 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt 900 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc 960 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg 1020 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga 1080 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc 1140 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa 1200 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa 1260 tttgatctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta 1320 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag 1380 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc 1440 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta 1500 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg 1560 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc 1620 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac 1680 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac 1740 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg 1800 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga 1860 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt 1920 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag 1980 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct 2040 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg 2100 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat 2160 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc 2220 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg 2280 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct 2340 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca 2400 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg 2460 ccagttaata gtttgcgcaa cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg 2520 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc 2580 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag 2640 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg 2700 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag 2760 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac gggataatac cgcgccacat 2820 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg 2880 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca 2940 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca 3000 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat 3060 tatgtaagca gacagtttta ttgttcatga tgatatattt ttatcttgtg caatgtaaca 3120 tcagagattt tgagacacaa cgtggctttg ttgaataaat cgaacttttg ctgagttgaa 3180 ggatcagatc acgcatcttc ccgacaacgc agaccgttcc gtggcaaagc aaaagttcaa 3240 aatcaccaac tggtccacct acaacaaagc tctcatcaac cgtggctccc tcactttctg 3300 gctggatgat ggggcgattc aggcctggta tgagtcagca acaccttctt cacgaggcag 3360 acctcagcgc cggtgatgcc ggccacgatg cgtccggcgt agaggatctc tcacctacca 3420 aacaatgccc ccctgcaaaa aataaattca tataaaaaac atacagataa ccatctgcgg 3480 tgataaatta tctctggcgg tgttgacata aataccactg gcggtgatac tgagcacatc 3540 agcaggacgc actgaccacc atgaaggtga cgctcttaaa attaagccct gaagaagggc 3600 agcattcaaa gcagaaggct ttggggtgtg tgatacgaaa cgaagcattg gaa 3653 //