LOCUS       pPLa831                 2971 bp    DNA     circular     27-MAR-2009
DEFINITION  LMBP 340.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 340
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 27-MAR-2009
            
            
COMMENT     VNTNAME|pPLa831|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pPLa831" 0 2971 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 1 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "AluI" "Alw44I" "HaeII" "HincII" "HindIII" "NaeI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 1 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 117 247 1 2971 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "AluI" "agct" 3 0 7 385 0 1167 0 1617 0 1997 0 
COMMENT            2133 0 2390 0 2911 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "Alw44I" "gtgcac" 2 0 1 2143 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 3 288 0 1718 0 2077 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HincII" "gtyrac" 4 0 1 150 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1165 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NaeI" "gccggc" 4 0 1 274 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "ColEI ori" 33 0 1 1716 2107 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1720..2108)") 
COMMENT        #7=(CFSignal (CObList) "kanamycin resistance" 4 0 0 615 1430 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "615..1430") 
COMMENT        #8=(CFSignal (CObList) "PL promoter" 30 0 1 117 247 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(117..247)")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #9=(CFSignal (CObList) "Last modified: 27-MAR-2009" 2 0 0 0 0 0 
COMMENT            (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #10=(CFSignal (CObList) "LMBP 340" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #14=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pPLa831'" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 340." 
COMMENT                          1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #17=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 03/27/09 09:53" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 2971 bp" 1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #22=(CFolderPar 
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COMMENT                    (CObjectList 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #25=(CRefLinePar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "Last modified: 27-MAR-2009") 
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COMMENT                                 "" 1))) "LMBP 340"))) "Annotations") 
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COMMENT                     #32=(CFolderPar 
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COMMENT                           (CObjectList 
COMMENT                            #33=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 615   End: 1430" 1) 
COMMENT                                   #35=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Tn903 antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #36=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #37=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   615..1430" 1))) 
COMMENT                                 "kanamycin resistance"))) "CDS (1 total)") 
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COMMENT                           (CObjectList 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 117   End: 247  (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "phage \361 major leftward promoter" 1) 
COMMENT                                   #42=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(117..247)" 1))) 
COMMENT                                 "PL promoter"))) 
COMMENT                          "Promoter Prokaryotic (1 total)") 
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COMMENT                           (CObjectList 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1716  End: 2107 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #47=(CLinePar 
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COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1720..2108)" 1))) 
COMMENT                                 "ColEI ori"))) "Replication Origin (1 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                        " N1: 2143 " 1))))) "Alw44I: 1 site") 1 
COMMENT                          5 "GTGCAC" "CACGTG") 
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COMMENT                                        " N1: 150 " 1))))) "HincII: 1 site") 3 3 
COMMENT                          "GTYRAC" "CARYTG") 
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COMMENT                                        " N1: 1165 " 1))))) "HindIII: 1 site") 1 
COMMENT                          5 "AAGCTT" "TTCGAA") 
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COMMENT                          "GCCGGC" "CGGCCG") 
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COMMENT                                        " N1: 288 " 1) 
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COMMENT                                        " N2: 1718 " 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #2# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 2077 " 1))))) "HaeII: 3 sites") 5 
COMMENT                          1 "RGCGCY" "YCGCGR") 
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COMMENT                                        " N1: 385 " 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 1167 " 1) 
COMMENT                                   #72=(CLinePar 
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COMMENT                                        " N3: 1617 " 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 4) 1 2 2 0 191) 
COMMENT                                        " N4: 1997 " 1) 
COMMENT                                   #74=(CLinePar 
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COMMENT                                        " N5: 2133 " 1) 
COMMENT                                   #75=(CLinePar 
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COMMENT                                        " N6: 2390 " 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 7) 1 2 2 0 191) 
COMMENT                                        " N7: 2911 " 1))))) "AluI: 7 sites") 2 2 
COMMENT                          "AGCT" "TCGA"))) 
COMMENT                   "Restriction/Methylation Map:  (no less than 1 site)") 
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COMMENT                          1) 
COMMENT                     #79=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "is calculated as an AVERAGE similarity" 1))) 
COMMENT                   "Motifs (both strands)") 
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COMMENT                          2910) 
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COMMENT                           " 450: pPLa831: AluI(1167) - AluI(1617)" 1) 4 "" 1167 
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COMMENT                           " 380: pPLa831: AluI(1617) - AluI(1997)" 1) 4 "" 1617 
COMMENT                          1996) 
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COMMENT                           " 257: pPLa831: AluI(2133) - AluI(2390)" 1) 4 "" 2133 
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COMMENT                           " 136: pPLa831: AluI(1997) - AluI(2133)" 1) 4 "" 1997 
COMMENT                          2132))) "Restriction Fragments")))) 
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FEATURES             Location/Qualifiers
     rep_origin      complement(1716..2107)
                     /vntifkey="33"
                     /label=ColEI\ori
                     /note="origin plasmid"
     CDS             615..1430
                     /vntifkey="4"
                     /label=kanamycin\resistance
                     /note="Tn903 antibiotic resistance gene; coding sequence"
     promoter        complement(117..247)
                     /vntifkey="30"
                     /label=PL\promoter
                     /note="phage ñ major leftward promoter"
BASE COUNT      752 a       703 c       717 g       799 t 
ORIGIN
        1 ttccaatgct tcgtttcgta tcacacaccc caaagccttc tgctttgaat gctgcccttc 
       61 ttcagggctt aattttaaga gcgtcacctt catggtggtc agtgcgtcct gctgatgtgc 
      121 tcagtatcac cgccagtggt atttatgtca acaccgccag agataattta tcaccgcaga 
      181 tggttatctg tatgtttttt atatgaattt attttttgca ggggggcatt gtttggtagg 
      241 tgagagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgctga ggtctgcctc 
      301 gtgaagaagg tgttgctgac tcataccagg cctgaatcgc cccatcatcc agccagaaag 
      361 tgagggagcc acggttgatg agagctttgt tgtaggtgga ccagttggtg attttgaact 
      421 tttgctttgc cacggaacgg tctgcgttgt cgggaagatg cgtgatctga tccttcaact 
      481 cagcaaaagt tcgatttatt caacaaagcc acgttgtgtc tcaaaatctc tgatgttaca 
      541 ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac ataaacagta 
      601 atacaagggg tgttatgagc catattcaac gggaaacgtc ttgctcgagg ccgcgattaa 
      661 attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat gtcgggcaat 
      721 caggtgcgac aatctatcga ttgtatggga agcccgatgc gccagagttg tttctgaaac 
      781 atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta aactggctga 
      841 cggaatttat gcctcttccg accatcaagc attttatccg tactcctgat gatgcatggt 
      901 tactcaccac tgcgatcccc gggaaaacag cattccaggt attagaagaa tatcctgatt 
      961 caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcat tcgattcctg 
     1021 tttgtaattg tccttttaac agcgatcgcg tatttcgtct cgctcaggcg caatcacgaa 
     1081 tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc tggcctgttg 
     1141 aacaagtctg gaaagaaatg cataagcttt tgccattctc accggattca gtcgtcactc 
     1201 atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata ggttgtattg 
     1261 atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta tggaactgcc 
     1321 tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt attgataatc 
     1381 ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa tcagaattgg 
     1441 ttaattggtt gtaacactgg cagagcatta cgctgacttg acgggacggc ggctttgttg 
     1501 aataaatcga acttttgctg agttgaagga tcagatcacg catcttcccg acaacgcaga 
     1561 ccgttccgtg gcaaagcaaa agttcaaaat caccaactgg tccacctaca acaaagctct 
     1621 catcaaccgt ggctccctca ctttctggct ggatgatggg gcgattcagg cctggtatga 
     1681 gtcagcaaca ccttcttcac gaggcagacc tcagcgcttt tccgcttcct cgctcactga 
     1741 ctcgctacgc tcggtcgttc gactgcggcg agcggtactg actcacacaa aaacggtaac 
     1801 acagttatcc acagaatcag gggataaggc cggaaagaac atgtgagcaa aagaccagga 
     1861 acaggaagaa ggccacgtag caggcgtttt tccataggct ccgcccccct gacgagcatc 
     1921 acaaaaatag acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     1981 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     2041 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt 
     2101 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     2161 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     2221 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     2281 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     2341 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     2401 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     2461 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     2521 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     2581 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     2641 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     2701 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     2761 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     2821 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     2881 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     2941 tgcgcaacgt tgttgccatc cggatccgga a 
//
