ID pIRES-LUC standard; circular DNA; ; 5453 BP. DE LMBP 3472. CC Accession number: LMBP 3472 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 15-APR-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT rep_origin 4..459 FT /vntifkey="33" FT /label=f1\ori FT promoter 626..642 FT /vntifkey="30" FT /label=T7\promoter FT terminator complement(759..855) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 759..855 FT /vntifkey="43" FT /label=SV40\polyA\early FT CDS complement(943..2595) FT /vntifkey="4" FT /label=LUCm FT /note="mutated firefly luciferase coding sequence" FT RBS complement(2609..3196) FT /vntifkey="32" FT /label=5'\UTR\of\the\EMCV\polyprotein\gene\(IRES) FT promoter complement(3267..3283) FT /vntifkey="30" FT /label=T3\promoter FT promoter complement(3347..3430) FT /vntifkey="30" FT /label=lac\promoter FT rep_origin complement(3522..3924) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(4465..5325) FT /vntifkey="4" FT /label=ampicillin\resistance SQ Sequence 5453 BP; 1343 A; 1373 C; 1304 G; 1433 t; ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 60 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 120 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 180 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 240 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 300 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 360 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 420 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 480 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 540 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 600 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg 660 gccccccctc gacggatcct tatcgatttt accacatttg tagaggtttt acttgcttta 720 aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt 780 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca 840 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct 900 tatcatgtct gctcgaagcg gccggccgcc ccgactctag aattacacgg cgatctttcc 960 gcccttcttg gcctttatga ggatctctct gatttttctt gcgtcgagtt ttccggtaag 1020 acctttcggt acttcgtcca caaacacaac tcctccgcgc aactttttcg cggttgttac 1080 ttgactggcg acgtaatcca cgatctcttt ttccgtcatc gtctttccgt gctccaaaac 1140 aacaacggcg gcgggaagtt caccggcgtc atcgtcggga agacctgcga cacctgcgtc 1200 gaagatgttg gggtgttgga gcaagatgga ttccaattca gcgggagcca cctgatagcc 1260 tttgtactta atcagagact tcaggcggtc aacgatgaag aagtgttcgt cttcgtccca 1320 gtaagctatg tctccagaat gtagccatcc atccttgtca atcaaggcgt tggtcgcttc 1380 cggattgttt acataaccgg acataatcat aggacctctc acacacagtt cgcctctttg 1440 attaacgccc agcgttttcc cggtatccag atccacaacc ttcgcttcaa aaaatggaac 1500 aactttaccg accgcgcccg gtttatcatc cccctcgggt gtaatcagaa tagctgatgt 1560 agtctcagtg agcccatatc cttgcctgat acctggcaga tggaacctct tggcaaccgc 1620 ttccccgact tccttagaga ggggagcgcc accagaagca atttcgtgta aattagataa 1680 atcgtatttg tcaatcagag tgcttttggc gaagaaggag aatagggttg gcaccagcag 1740 cgcactttga atcttgtaat cctgaaggct cctcagaaac agctcttctt caaatctata 1800 cattaagacg actcgaaatc cacatatcaa atatccgagt gtagtaaaca ttccaaaacc 1860 gtgatggaat ggaacaacac ttaaaatcgc agtatccgga atgatttgat tgccaaaaat 1920 aggatctctg gcatgcgaga atctcacgca ggcagttcta tgaggcagag cgacaccttt 1980 aggcagacca gtagatccag aggagttcat gatcagtgca attgtcttgt ccctatcgaa 2040 ggactctggc acaaaatcgt attcattaaa accgggaggt agatgagatg tgacgaacgt 2100 gtacatcgac tgaaatccct ggtaatccgt tttagaatcc atgataataa ttttttggat 2160 gattgggagc tttttttgca cgttcaaaat tttttgcaac ccctttttgg aaacgaacac 2220 cacggtaggc tgcgaaatgc ccatactgtt gagcaattca cgttcattat aaatgtcgtt 2280 cgcgggcgca actgcaactc cgataaataa cgcgcccaac accggcataa agaattgaag 2340 agagttttca ctgcatacga cgattctgtg atttgtattc agcccatatc gtttcatagc 2400 ttctgccaac cgaacggaca tttcgaagta ctcagcgtaa gtgatgtcca cctcgatatg 2460 tgcatctgta aaagcaattg ttccaggaac cagggcgtat ctcttcatag ccttatgcag 2520 ttgctctcca gcggttccat cttccagcgg atagaatggc gccgggcctt tctttatgtt 2580 tttggcgtct tccatggttg tggcaagctt atcatcgtgt ttttcaaagg aaaaccacgt 2640 ccccgtggtt cggggggcct agacgttttt taacctcgac taaacacatg taaagcatgt 2700 gcaccgaggc cccagatcag atcccataca atggggtacc ttctgggcat ccttcagccc 2760 cttgttgaat acgcttgagg agagccattt gactctttcc acaactatcc aactcacaac 2820 gtggcactgg ggttgtgccg cctttgcagg tgtatcttat acacgtggct tttggccgca 2880 gaggcacctg tcgccaggtg gggggttccg ctgcctgcaa agggtcgcta cagacgttgt 2940 ttgtcttcaa gaagcttcca gaggaactgc ttccttcacg acattcaaca gaccttgcat 3000 tcctttggcg agaggggaaa gacccctagg aatgctcgtc aagaagacag ggccaggttt 3060 ccgggccctc acattgccaa aagacggcaa tatggtggaa aataacatat agacaaacgc 3120 acaccggcct tattccaagc ggcttcggcc agtaacgtta gggggggggg agggagaggg 3180 gggggggggg ggggggcgga attctctaga ggatccacta gttctagagc ggccgccacc 3240 gcggtggagc tccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc 3300 atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg 3360 agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat 3420 tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg 3480 aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct 3540 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 3600 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 3660 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 3720 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 3780 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 3840 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 3900 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 3960 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 4020 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 4080 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 4140 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 4200 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 4260 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 4320 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 4380 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 4440 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 4500 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 4560 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 4620 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 4680 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 4740 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg 4800 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 4860 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 4920 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 4980 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 5040 atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc 5100 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 5160 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 5220 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 5280 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 5340 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 5400 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cac 5453 //