ID pJBtrc10 standard; circular DNA; ; 8463 BP. DE LMBP 3523. CC Accession number: LMBP 3523 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 8-OCT-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS complement(793..1983) FT /vntifkey="4" FT /label=tetracycline\resistance FT CDS complement(2696..3844) FT /vntifkey="4" FT /label=trfA FT /note="P382 coding sequence; P285 starts at 3553" FT promoter complement(3953..4002) FT /vntifkey="30" FT /label=Tn5\neomycin\resistance\promoter FT rep_origin complement(4242..4766) FT /vntifkey="33" FT /label=RK2\ori FT promoter 5249..5326 FT /vntifkey="30" FT /label=lacIq\promoter FT /note="mutated E. coli repressor lacI promoter, incl. RBS" FT CDS 5327..6409 FT /vntifkey="4" FT /label=lacI FT /note="E. coli repressor lacI coding sequence" FT promoter 6632..6675 FT /vntifkey="30" FT /label=trc\promoter FT RBS 6701..6742 FT /vntifkey="32" FT /label=5'\UTR\of\T7\gene\10,\incl.\RBS FT /note="5' UTR of phage T7 gene 10, incl. RBS" FT promoter complement(6799..6815) FT /vntifkey="30" FT /label=T3\promoter FT terminator 6833..6880 FT /vntifkey="43" FT /label=T7\terminator FT /note="phage T7 gene 10 terminator" FT terminator 6954..7001 FT /vntifkey="43" FT /label=T7\terminator FT /note="phage T7 gene 10 terminator" FT terminator 7070..7151 FT /vntifkey="43" FT /label=fd\terminator FT /note="phage fd terminator" FT terminator 7180..7261 FT /vntifkey="43" FT /label=fd\terminator FT /note="phage fd terminator" FT rep_origin complement(8176..8286) FT /vntifkey="33" FT /label=RK2/RP4\oriT FT misc_feature 6676..6700 FT /vntifkey="21" FT /label=5'\UTR\of\lacZ SQ Sequence 8463 BP; 1876 A; 2319 C; 2303 G; 1965 t; tcctgcggta aagctcatca gcgtggtcgt gaagcgattc acagatgtct gcctgttcat 60 ccgcgtccag ctcgttgagt ttctccagaa gcgttaatgt ctggcttctg ataaagcggg 120 ccatgttaag ggcggttttt tcctgtttgg tcactgatgc ctccgtgtaa gggggatttc 180 tgttcatggg ggtaatgata ccgatgaaac gagagaggat gctcacgata cgggttactg 240 atgatgaaca tgcccggtta ctggaacgtt gtgagggtaa acaactggcg gtatggatgc 300 ggcgggacca gagaaaaatc actcagggtc aatgccagcg cttcgttaat acagatgtag 360 gtgttccaca gggtagccag cagcatcctg cgatgcagat ccggaacata atggtgcagg 420 gcgctgactt ccgcgtttcc agactttacg aaacacggaa accgaagacc attcatgttg 480 ttgctcaggt cgcagacgtt ttgcagcagc agtcgcttca cgttcgctcg cgtatcggtg 540 attcattctg ctaaccagta aggcaacccc gccagcctag ccgggtcctc aacgacagga 600 gcacgatcat gcgcacccgt ggccaggacc caacgctgcc cgagatgcgc cgcgtgcggc 660 tgctggagat ggcggacgcg atggatatgt tctgccaagg gttggtttgc gcattcacag 720 ttctccgcaa gaattgattg gctccaattc ttggagtggt gaatccgtta gcgaggtgcc 780 gccggcttcc attcaggtcg aggtggcccg gctccatgca ccgcgacgca acgcggggag 840 gcagacaagg tatagggcgg cgcctacaat ccatgccaac ccgttccatg tgctcgccga 900 ggcggcataa atcgccgtga cgatcagcgg tccagtgatc gaagttaggc tggtaagagc 960 cgcgagcgat ccttgaagct gtccctgatg gtcgtcatct acctgcctgg acagcatggc 1020 ctgcaacgcg ggcatcccga tgccgccgga agcgagaaga atcataatgg ggaaggccat 1080 ccagcctcgc gtcgcgaacg ccagcaagac gtagcccagc gcgtcggccg ccatgccggc 1140 gataatggcc tgcttctcgc cgaaacgttt ggtggcggga ccagtgacga aggcttgagc 1200 gagggcgtgc aagattccga ataccgcaag cgacaggccg atcatcgtcg cgctccagcg 1260 aaagcggtcc tcgccgaaaa tgacccagag cgctgccggc acctgtccta cgagttgcat 1320 gataaagaag acagtcataa gtgcggcgac gatagtcatg ccccgcgccc accggaagga 1380 gctgactggg ttgaaggctc tcaagggcat cggtcgacgc tctcccttat gcgactcctg 1440 cattaggaag cagcccagta gtaggttgag gccgttgagc accgccgccg caaggaatgg 1500 tgcatgcaag gagatggcgc ccaacagtcc cccggccacg gggcctgcca ccatacccac 1560 gccgaaacaa gcgctcatga gcccgaagtg gcgagcccga tcttccccat cggtgatgtc 1620 ggcgatatag gcgccagcaa ccgcacctgt ggcgccggtg atgccggcca cgatgcgtcc 1680 ggcgtagagg atccacagga cgggtgtggt cgccatgatc gcgtagtcga tagtggctcc 1740 aagtagcgaa gcgagcagga ctgggcggcg gccaaagcgg tcggacagtg ctccgagaac 1800 gggtgcgcat agaaattgca tcaacgcata tagcgctagc agcacgccat agtgactggc 1860 gatgctgtcg gaatggacga tatcccgcaa gaggcccggc agtaccggca taaccaagcc 1920 tatgcctaca gcatccaggg tgacggtgcc gaggatgacg atgagcgcat tgttagattt 1980 catacacggt gcctgactgc gttagcaatt taactgtgat aaactaccgc attaaagctt 2040 atcgatgata agctgtcaaa catgagaatt cactggccgt cgttttacaa cgtcgtgact 2100 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct 2160 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg 2220 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 2280 tatatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca 2340 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag 2400 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa 2460 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat 2520 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 2580 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 2640 gcttcaataa tcatgcaggt gctgaacccc cagccggaac tgaccccaca aggccctagc 2700 gtttgcaatg caccaggtca tcattgaccc aggcgtgttc caccaggccg ctgcctcgca 2760 actcttcgca ggcttcgccg acctgctcgc gccacttctt cacgcgggtg gaatccgatc 2820 cgcacatgag gcggaaggtt tccagcttga gcgggtacgg ctcccggtgc gagctgaaat 2880 agtcgaacat ccgtcgggcc gtcggcgaca gcttgcggta cttctcccat atgaatttcg 2940 tgtagtggtc gccagcaaac agcacgacga tttcctcgtc gatcaggacc tggcaacggg 3000 acgttttctt gccacggtcc aggacgcgga agcggtgcag cagcgacacc gattccaggt 3060 gcccaacgcg gtcggacgtg aagcccatcg ccgtcgcctg taggcgcgac aggcattcct 3120 cggccttcgt gtaataccgg ccattgatcg accagcccag gtcctggcaa agctcgtaga 3180 acgtgaaggt gatcggctcg ccgatagggg tgcgcttcgc gtactccaac acctgctgcc 3240 acaccagttc gtcatcgtcg gcccgcagct cgacgccggt gtaggtgatc ttcacgtcct 3300 tgttgacgtg gaaaatgacc ttgttttgca gcgcctcgcg cgggattttc ttgttgcgcg 3360 tggtgaacag ggcagagcgg gccgtgtcgt ttggcatcgc tcgcatcgtg tccggccacg 3420 gcgcaatatc gaacaaggaa agctgcattt ccttgatctg ctgcttcgtg tgtttcagca 3480 acgcggcctg cttggcctcg ctgacctgtt ttgccaggtc ctcgccggcg gtttttcgct 3540 tcttggtcgt catagttcct cgcgtgtcga tggtcatcga cttcgccaaa cctgccgcct 3600 cctgttcgag acgacgcgaa cgctccacgg cggccgatgg cgcgggcagg gcagggggag 3660 ccagttgcac gctgtcgcgc tcgatcttgg ccgtagcttg ctggaccatc gagccgacgg 3720 actggaaggt ttcgcggggc gcacgcatga cggtgcggct tgcgatggtt tcggcatcct 3780 cggcggaaaa ccccgcgtcg atcagttctt gcctgtatgc cttccggtca aacgtccgat 3840 tcattcaccc tccttgcggg attgccccgg aattaattcc ccggatcgat ccgtcgatct 3900 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg 3960 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa 4020 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc 4080 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg 4140 tttctgcgga ctggctttct acgtggctgc catttttggg gtgaggtcgt tcgcggccga 4200 ggggcgcagc ccctgggggg atggggtgcc gcgttagcgg gccgggaggg ttcgagaagg 4260 gggggcaccc cccttcggcg tgcgcggtca cgcgccaggg cgcagccctg gttaaaaaca 4320 aggtttataa atattggttt aaaagcaggt taaaagacag gttagcggtg gccgaaaaac 4380 gggcggaaac ccttgcaaat gctggatttt ctgcctgtgg acagcccctc aaatgtcaat 4440 aggtgcgccc ctcatctgtc atcactctgc ccctcaagtg tcaaggatcg cgcccctcat 4500 ctgtcagtag tcgcgcccct caagtgtcaa taccgcaggg cacttatccc caggcttgtc 4560 cacatcatct gtgggaaact cgcgtaaaat caggcgtttt cgccgatttg cgaggctggc 4620 cagctccacg tcgccggccg aaatcgagcc tgcccctcat ctgtcaacgc cgcgccgggt 4680 gagtcggccc ctcaagtgtc aacgtccgcc cctcatctgt cagtgagggc caagttttcc 4740 gcgtggtatc cacaacgccg gcggccctac atggctctgc tgtagtgagt gggttgcgct 4800 ccggcagcgg tcctgatccc ccgcagaaaa aaaggatctc aagaagatcc tttgatcttt 4860 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 4920 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 4980 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 5040 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactcttaag ctgcaatgat 5100 accgttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca 5160 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag gcagcagatc aattcgcgcg 5220 cgaaggcgaa gcggcatgca tttacgttga caccatcgaa tggtgcaaaa cctttcgcgg 5280 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga aaccagtaac 5340 gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa 5400 ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct 5460 gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg 5520 cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc 5580 tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga 5640 agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa 5700 ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc 5760 gttatttctt gatgtctctg accagacacc catcaacagt attattttct cccatgaaga 5820 cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt 5880 agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct 5940 cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg 6000 ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc 6060 caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg 6120 tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc 6180 gtcaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct 6240 gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa 6300 aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc 6360 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa 6420 ttaatgtgag ttagcgcgaa ttgatctggt ttgacagctt atcatcgact gcacggtgca 6480 ccaatgcttc tggcgtcagg cagccatcgg aagctgtggt atggctgtgc aggtcgtaaa 6540 tcactgcata attcgtgtcg ctcaaggcgc actcccgttc tggataatgt tttttgcgcc 6600 gacatcataa cggttctggc aaatattctg aaatgagctg ttgacaatta atcatccggc 6660 tcgtataatg tgtggaattg tgagcggata acaatttcac tctagaaata attttgttta 6720 actttaagaa ggagatatac atatgggccc ggatccctcg aggtcgacga attcgagctc 6780 ggccgacttg gccttccctt tagtgagggt taataaactt ggtgagcaat aactagcata 6840 accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag gaactatatg 6900 cgctcatacg atatgaacgt tgagactgcc gctgagttat cagtgagcaa taactagcat 6960 aaccccttgg ggcctctaaa cgggtcttga ggggtttttt gctgaaagga ggaactatat 7020 ccggatagct tatcgctaga ggtcgaaatt cacctcgaaa gcaagctgat aaaccgatac 7080 aattaaaggc tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta 7140 ttcgcaattc caagctaatt cacctcgaaa gcaagctgat aaaccgatac aattaaaggc 7200 tccttttgga gccttttttt ttggagattt tcaacgtgaa aaaattatta ttcgcaattc 7260 caagctctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc 7320 ggagacggtc acagcttgtc tgtaagcgga tgcagatcac gcgccctgta gcggcgcatt 7380 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc 7440 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca 7500 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc 7560 caaaaaactt gattagggtg atggttcaca ctcaaccaag tcattctgag aatagtgtat 7620 gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag 7680 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt 7740 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc 7800 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa 7860 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg 7920 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa 7980 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgggt cgatcgacgg 8040 atcttttccg ctgcataacc ctgcttcggg gtcattatag cgattttttc ggtatatcca 8100 tcctttttcg cacgatatac aggattttgc caaagggttc gtgtagactt tccttggtgt 8160 atccaacggc gtcagccggg caggataggt gaagtaggcc cacccgcgag cgggtgttcc 8220 ttcttcactg tcccttattc gcacctggcg gtgctcaacg ggaatcctgc tctgcgaggc 8280 tggccgtaag ctctaagaaa ccattattat catgacatta acctataaaa ataggcgtat 8340 cacgaggccc tttcgtcttc aagaattaat tcactggccg tcgttttaca acgtcgtgac 8400 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc 8460 aga 8463 //