LOCUS       pJBLT10lacZ            11114 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 3589.
SOURCE      
  ORGANISM  
COMMENT     (800)357-3114 or http://www.informaxinc.com/
COMMENT     VNTNAME|pJBLT10lacZ|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pJBLT10lacZ" 0 11114 
COMMENT       (CDBMol 0 0 1 5574 1 0 0 1 1 "" "" 0 0 0 0 (CObList) (CObList) 
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COMMENT             (CTerm 1 0 0 "StuI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "BglII" "" 1 0 0 0 1 0 0 0 0 0) 1 0) 
COMMENT         #1=(CFragment 3 1 1 0 0 "" 0 "" "" "" "" "" "" "pBBRLT10lacZ" "" 0 0 1 
COMMENT             5539 3 5541 4142 265 0 "" "" "" "" "" "" (CObList) 
COMMENT             (CTerm 1 0 0 "ScaI" "" 1 0 0 0 0 0 0 0 0 0) 
COMMENT             (CTerm 1 1 0 "EagI" "" 1 0 0 0 1 0 0 0 0 0) 1 0)) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 "MAIN" 1 1 1 1 0 0 1 1 0 1 10 5 40 50 0 1 0 
COMMENT        (CHomObj 0 0 0 3 75) (CWordArray) (CWordArray) 
COMMENT        (CStringList "ApaLI" "AvaI" "BamHI" "ClaI" "EcoRI" "HindIII" "NcoI" 
COMMENT         "PstI" "SmaI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 11114 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT            10428 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 5 2543 0 3986 0 4524 0 6290 
COMMENT            0 6564 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 1211 0 6732 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "ClaI" "atcgat" 3 0 4 2029 0 4635 0 6473 0 8561 
COMMENT            0 0 "") 
COMMENT        #6=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 2 1103 0 6741 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 6044 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 5426 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 6724 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "SmaI" "cccggg" 4 0 1 6292 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 6290 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #13=(CFSignal (CObList) "trfA" 4 0 1 7370 8518 1 (CStringList) 
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COMMENT        #15=(CFSignal (CObList) "oriV" 33 0 1 8916 9440 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #16=(CFSignal (CObList) "AMP" 4 0 1 9687 10547 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #17=(CFSignal (CObList) "oriT" 33 0 1 10827 10938 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "T7 terminator" 43 0 0 176 223 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #19=(CFSignal (CObList) "T7 terminator" 43 0 0 55 102 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "T3 promoter" 30 0 1 21 38 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "fd terminator" 43 0 0 379 488 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #22=(CFSignal (CObList) "fd terminator" 43 0 0 269 378 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "T7 promoter" 30 0 0 1128 1144 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #24=(CFSignal (CObList) "T7g10 RBS" 32 0 0 1166 1207 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #25=(CFSignal (CObList) "lacZ" 4 0 0 1208 4261 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #26=(CFSignal (CObList) "T3 promoter" 30 0 1 4696 4713 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #27=(CFSignal (CObList) "T7 terminator" 43 0 0 4730 4777 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #28=(CFSignal (CObList) "T7 terminator" 43 0 0 4851 4898 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #29=(CFSignal (CObList) "fd terminator" 43 0 0 4944 5053 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #30=(CFSignal (CObList) "fd terminator" 43 0 0 5054 5163 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #31=(CFSignal (CObList) "PL promoter" 30 0 0 858 988 1 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) (CObList) (CObList) (CObList) 
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COMMENT                          "DNA pJBLT10lacZ" 0) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "DMB 3589" 0) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 11114 bp" 0) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Constructed" 0) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 0))) "General Description") 
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COMMENT                                 0) 
COMMENT                            #42=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "length: 5575" 0) 
COMMENT                            #43=(CLinePar (CParagraph 0 (0 0) 1 2 2 0 180) 
COMMENT                                 "molecule position: from 5542 to 2" 0) 
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COMMENT                                        "  StuI site #1" 0))) "Left Terminus") 
COMMENT                            #46=(CFolderPar 
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COMMENT                                        "  BglII site #1" 0) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "    Completely filled in" 0))) 
COMMENT                                 "Right Terminus"))) "#1: FRAGMENT of pJB3kan1 ") 
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COMMENT                          (CGroupPar (CParagraph 0 (5 #1# 0) 1 2 1 0 195) 
COMMENT                           (CObjectList 
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COMMENT                                 "parent position: from 4142 to 265" 0) 
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COMMENT                                 "length: 5539" 0) 
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COMMENT                                 "molecule position: from 3 to 5541" 0) 
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COMMENT                                        "  ScaI site #1" 0))) "Left Terminus") 
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COMMENT                                        "  EagI site #1" 0) 
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COMMENT                                        "    Completely filled in" 0))) 
COMMENT                                 "Right Terminus"))) 
COMMENT                          "#2: FRAGMENT of pBBRLT10lacZ (Complementary)"))) 
COMMENT                   "Component Fragments") 
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COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
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COMMENT                                        "first 8 aa are missing (derived from pMC1403)" 
COMMENT                                        0))) "lacZ") 
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COMMENT                                   #65=(CLinePar 
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COMMENT                                        "antibiotic resistance gene" 0))) "KAN") 
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COMMENT                                   #68=(CLinePar 
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COMMENT                                        "trfA gene (encoding P382)" 0))) "trfA") 
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COMMENT                          "CDS (4 signals)") 
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COMMENT                                        "Start: 1128  End: 1144" 0) 
COMMENT                                   #81=(CLinePar 
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COMMENT                                        "PHAGE T7 gene 10 promoter" 0))) 
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COMMENT                                   #84=(CLinePar 
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COMMENT                                   #87=(CLinePar 
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COMMENT                                        "promoter" 0))) "neo promoter"))) 
COMMENT                          "Promoter_prokaryotic (5 signals)") 
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COMMENT                                        "VEGETATIVE REPLICATION origin" 0))) 
COMMENT                                 "oriV") 
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COMMENT                             "ApaLI: 4 sites") 1 5 "GTGCAC" "CACGTG") 
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COMMENT                                            " N5: 6564 " 0))))) "AvaI: 5 sites") 
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FEATURES             Location/Qualifiers
     CDS             complement(5782..6597)
                     /vntifkey="4"
                     /label=KAN
                     /note="antibiotic resistance gene"
     CDS             complement(7370..8518)
                     /vntifkey="4"
                     /label=trfA
                     /note="trfA gene (encoding P382)"
     promoter        complement(8627..8839)
                     /vntifkey="30"
                     /label=neo\promoter
                     /note="promoter"
     rep_origin      complement(8916..9440)
                     /vntifkey="33"
                     /label=oriV
                     /note="VEGETATIVE REPLICATION origin"
     CDS             complement(9687..10547)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene"
     rep_origin      complement(10827..10938)
                     /vntifkey="33"
                     /label=oriT
                     /note="CONJUGATIONAL DNA TRANSFER origin"
     terminator      176..223
                     /vntifkey="43"
                     /label=T7\terminator
                     /note="T7 gene 10 terminator"
     terminator      55..102
                     /vntifkey="43"
                     /label=T7\terminator
                     /note="T7 gene 10 terminator"
     promoter        complement(21..38)
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="promoter"
     terminator      379..488
                     /vntifkey="43"
                     /label=fd\terminator
                     /note="fd terminator"
     terminator      269..378
                     /vntifkey="43"
                     /label=fd\terminator
                     /note="fd terminator"
     promoter        1128..1144
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="PHAGE T7 gene 10 promoter"
     RBS             1166..1207
                     /vntifkey="32"
                     /label=T7g10\RBS
     CDS             1208..4261
                     /vntifkey="4"
                     /label=lacZ
                     /note="first 8 aa are missing (derived from pMC1403)"
     promoter        complement(4696..4713)
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="promoter"
     terminator      4730..4777
                     /vntifkey="43"
                     /label=T7\terminator
                     /note="T7 gene 10 terminator"
     terminator      4851..4898
                     /vntifkey="43"
                     /label=T7\terminator
                     /note="T7 gene 10 terminator"
     terminator      4944..5053
                     /vntifkey="43"
                     /label=fd\terminator
                     /note="fd terminator"
     terminator      5054..5163
                     /vntifkey="43"
                     /label=fd\terminator
                     /note="fd terminator"
     promoter        858..988
                     /vntifkey="30"
                     /label=PL\promoter
                     /note="promoter"
BASE COUNT     2562 a      2946 c      2827 g      2773 t         6 others
ORIGIN
        1 tcggccgact tggccttccc tttagtgagg gttaataaac ttggtgagca ataactagca 
       61 taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata 
      121 tgcgctcata cgatatgaac gttgagactg ccgctgagtt atcagtgagc aataactagc 
      181 ataacccctt ggggcctcta aacgggtctt gaggggtttt ttgctgaaag gaggaactat 
      241 atccggatag cttatcgcta gaggtcgaaa ttcacctcga aagcaagctg ataaaccgat 
      301 acaattaaag gctccttttg gagccttttt ttttggagat tttcaacgtg aaaaaattat 
      361 tattcgcaat tccaagctaa ttcacctcga aagcaagctg ataaaccgat acaattaaag 
      421 gctccttttg gagccttttt ttttggagat tttcaacgtg aaaaaattat tattcgcaat 
      481 tccaagctct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc 
      541 ccggagacgg tcacagcttg tctgtaagcg gatgcagatc acgcgccctg tagcggcgca 
      601 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta 
      661 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt 
      721 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac 
      781 cccaaaaaac ttgattaggg tgatggttca cctggatatt acggcctttt tggccacgat 
      841 gcgtccggcg tagaggatct ctcacctacc aaacaatgcc cccctgcaaa aaataaattc 
      901 atataaaaaa catacagata accatctgcg gtgataaatt atctctggcg gtgttgacat 
      961 aaataccact ggcggtgata ctgagcacat cagcaggacg cactgaccac catgaaggtg 
     1021 acgctcttaa aattaagccc tgaagaaggg cagcattcaa agcagaaggc tttggggtgt 
     1081 gtgatacgaa acgaagcatt ggaattccgg atctcgatcc cggaaattaa tacgactcac 
     1141 tatagggaga ccacaacggt ttccctctag aaataatttt gtttaacttt aagaaggaga 
     1201 tatacatatg gatcccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca 
     1261 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg 
     1321 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt 
     1381 tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac 
     1441 tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt 
     1501 aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta 
     1561 ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt 
     1621 tgatggcgtt aactcggcgt ttcatctgtg gtgcaacggg cgctgggtcg gttacggcca 
     1681 ggacagtcgt ttgccgtctg aatttgacct gagcgcattt ttacgcgccg gagaaaaccg 
     1741 cctcgcggtg atggtgctgc gttggagtga cggcagttat ctggaagatc aggatatgtg 
     1801 gcggatgagc ggcattttcc gtgacgtctc gttgctgcat aaaccgacta cacaaatcag 
     1861 cgatttccat gttgccactc gctttaatga tgatttcagc cgcgctgtac tggaggctga 
     1921 agttcagatg tgcggcgagt tgcgtgacta cctacgggta acagtttctt tatggcaggg 
     1981 tgaaacgcag gtcgccagcg gcaccgcgcc tttcggcggt gaaattatcg atgagcgtgg 
     2041 tggttatgcc gatcgcgtca cactacgtct gaacgtcgaa aacccgaaac tgtggagcgc 
     2101 cgaaatcccg aatctctatc gtgcggtggt tgaactgcac accgccgacg gcacgctgat 
     2161 tgaagcagaa gcctgcgatg tcggtttccg cgaggtgcgg attgaaaatg gtctgctgct 
     2221 gctgaacggc aagccgttgc tgattcgagg cgttaaccgt cacgagcatc atcctctgca 
     2281 tggtcaggtc atggatgagc agacgatggt gcaggatatc ctgctgatga agcagaacaa 
     2341 ctttaacgcc gtgcgctgtt cgcattatcc gaaccatccg ctgtggtaca cgctgtgcga 
     2401 ccgctacggc ctgtatgtgg tggatgaagc caatattgaa acccacggca tggtgccaat 
     2461 gaatcgtctg accgatgatc cgcgctggct accggcgatg agcgaacgcg taacgcgaat 
     2521 ggtgcagcgc gatcgtaatc acccgagtgt gatcatctgg tcgctgggga atgaatcagg 
     2581 ccacggcgct aatcacgacg cgctgtatcg ctggatcaaa tctgtcgatc cttcccgccc 
     2641 ggtgcagtat gaaggcggcg gagccgacac cacggccacc gatattattt gcccgatgta 
     2701 cgcgcgcgtg gatgaagacc agcccttccc ggctgtgccg aaatggtcca tcaaaaaatg 
     2761 gctttcgcta cctggagaga cgcgcccgct gatcctttgc gaatacgccc acgcgatggg 
     2821 taacagtctt ggcggtttcg ctaaatactg gcaggcgttt cgtcagtatc cccgtttaca 
     2881 gggcggcttc gtctgggact gggtggatca gtcgctgatt aaatatgatg aaaacggcaa 
     2941 cccgtggtcg gcttacggcg gtgattttgg cgatacgccg aacgatcgcc agttctgtat 
     3001 gaacggtctg gtctttgccg accgcacgcc gcatccagcg ctgacggaag caaaacacca 
     3061 gcagcagttt ttccagttcc gtttatccgg gcaaaccatc gaagtgacca gcgaatacct 
     3121 gttccgtcat agcgataacg agctcctgca ctggatggtg gcgctggatg gtaagccgct 
     3181 ggcaagcggt gaagtgcctc tggatgtcgc tccacaaggt aaacagttga ttgaactgcc 
     3241 tgaactaccg cagccggaga gcgccgggca actctggctc acagtacgcg tagtgcaacc 
     3301 gaacgcgacc gcatggtcag aagccgggca catcagcgcc tggcagcagt ggcgtctggc 
     3361 ggaaaacctc agtgtgacgc tccccgccgc gtcccacgcc atcccgcatc tgaccaccag 
     3421 cgaaatggat ttttgcatcg agctgggtaa taagcgttgg caatttaacc gccagtcagg 
     3481 ctttctttca cagatgtgga ttggcgataa aaaacaactg ctgacgccgc tgcgcgatca 
     3541 gttcacccgt gcaccgctgg ataacgacat tggcgtaagt gaagcgaccc gcattgaccc 
     3601 taacgcctgg gtcgaacgct ggaaggcggc gggccattac caggccgaag cagcgttgtt 
     3661 gcagtgcacg gcagatacac ttgctgatgc ggtgctgatt acgaccgctc acgcgtggca 
     3721 gcatcagggg aaaaccttat ttatcagccg gaaaacctac cggattgatg gtagtggtca 
     3781 aatggcgatt accgttgatg ttgaagtggc gagcgataca ccgcatccgg cgcggattgg 
     3841 cctgaactgc cagctggcgc aggtagcaga gcgggtaaac tggctcggat tagggccgca 
     3901 agaaaactat cccgaccgcc ttactgccgc ctgttttgac cgctgggatc tgccattgtc 
     3961 agacatgtat accccgtacg tcttcccgag cgaaaacggt ctgcgctgcg ggacgcgcga 
     4021 attgaattat ggcccacacc agtggcgcgg cgacttccag ttcaacatca gccgctacag 
     4081 tcaacagcaa ctgatggaaa ccagccatcg ccatctgctg cacgcggaag aaggcacatg 
     4141 gctgaatatc gacggtttcc atatggggat tggtggcgac gactcctgga gcccgtcagt 
     4201 atcggcgnnn nnncagctga gcgccggtcg ctaccattac cagttggtct ggtgtcaaaa 
     4261 ataataataa ccgggcaggc catgtctgcc cgtatttcgc gtaaggaaat ccattatgta 
     4321 ctatttaaaa aacacaaact tttggatgtt cggtttattc tttttctttt acttttttat 
     4381 catgggagcc tacttcccgt ttttcccgat ttggctacat gacatcaacc atatcagcaa 
     4441 aagtgatacg ggtattattt ttgccgctat ttctctgttc tcgctattat tccaaccgct 
     4501 gtttggtctg ctttctgaca aactcgggct gcgcaaatac ctgctgtgga ttattaccgg 
     4561 catgttagtg atgtttgcgc cgttctttat ttttatcttc gggccactgt tacaatacaa 
     4621 cattttagta ggatcgattg ttggtggtat ttatctaggc ttttgtttta acgccggggc 
     4681 cgacttggcc ttccctttag tgagggttaa taaacttggt gagcaataac tagcataacc 
     4741 ccttggggcc tctaaacggg tcttgagggg ttttttgctg aaaggaggaa ctatatgcgc 
     4801 tcatacgata tgaacgttga gactgccgct gagttatcag tgagcaataa ctagcataac 
     4861 cccttggggc ctctaaacgg gtcttgaggg gttttttgct gaaaggagga actatatccg 
     4921 gatagcttat cgctagaggt cgaaattcac ctcgaaagca agctgataaa ccgatacaat 
     4981 taaaggctcc ttttggagcc tttttttttg gagattttca acgtgaaaaa attattattc 
     5041 gcaattccaa gctaattcac ctcgaaagca agctgataaa ccgatacaat taaaggctcc 
     5101 ttttggagcc tttttttttg gagattttca acgtgaaaaa attattattc gcaattccaa 
     5161 gctctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 
     5221 gacggtcaca gcttgtctgt aagcggatgc agatcacgcg ccctgtagcg gcgcattaag 
     5281 cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc 
     5341 cgctcctttc gctttcttcc cttcctttct cgccacgttc gccaaacgtg gccaatatgg 
     5401 acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc 
     5461 tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat gtcggcagaa 
     5521 tgcttaatga attacaacag tcctgaatcg ccccatcatc cagccagaaa gtgagggagc 
     5581 cacggttgat gagagctttg ttgtaggtgg accagttggt gattttgaac ttttgctttg 
     5641 ccacggaacg gtctgcgttg tcgggaagat gcgtgatctg atccttcaac tcagcaaaag 
     5701 ttcgatttat tcaacaaagc cgccgtcccg tcaagtcagc gtaatgctct gccagtgtta 
     5761 caaccaatta accaattctg attagaaaaa ctcatcgagc atcaaatgaa actgcaattt 
     5821 attcatatca ggattatcaa taccatattt ttgaaaaagc cgtttctgta atgaaggaga 
     5881 aaactcaccg aggcagttcc ataggatggc aagatcctgg tatcggtctg cgattccgac 
     5941 tcgtccaaca tcaatacaac ctattaattt cccctcgtca aaaataaggt tatcaagtga 
     6001 gaaatcacca tgagtgacga ctgaatccgg tgagaatggc aaaagcttat gcatttcttt 
     6061 ccagacttgt tcaacaggcc agccattacg ctcgtcatca aaatcactcg catcaaccaa 
     6121 accgttattc attcgtgatt gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg 
     6181 acaattacaa acaggaatcg aatgcaaccg gcgcaggaac actgccagcg catcaacaat 
     6241 attttcacct gaatcaggat attcttctaa tacctggaat gctgttttcc cggggatcgc 
     6301 agtggtgagt aaccatgcat catcaggagt acggataaaa tgcttgatgg tcggaagagg 
     6361 cataaattcc gtcagccagt ttagtctgac catctcatct gtaacatcat tggcaacgct 
     6421 acctttgcca tgtttcagaa acaactctgg cgcatcgggc ttcccataca atcgatagat 
     6481 tgtcgcacct gattgcccga cattatcgcg agcccattta tacccatata aatcagcatc 
     6541 catgttggaa tttaatcgcg gcctcgagca agacgtttcc cgttgaatat ggctcataac 
     6601 accccttgta ttactgttta tgtaagcaga cagttttatt gttcatgatg atatattttt 
     6661 atcttgtgca atgtaacatc agagattttg agacacaacg tggctttccc ccccccccct 
     6721 gcaggtcgac ggatccgggg aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa 
     6781 accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta 
     6841 atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat 
     6901 ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatatg 
     6961 gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc gacacccgcc 
     7021 aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc 
     7081 tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc 
     7141 gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt 
     7201 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 
     7261 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 
     7321 ataatcatgc aggtgctgaa cccccagccg gaactgaccc cacaaggccc tagcgtttgc 
     7381 aatgcaccag gtcatcattg acccaggcgt gttccaccag gccgctgcct cgcaactctt 
     7441 cgcaggcttc gccgacctgc tcgcgccact tcttcacgcg ggtggaatcc gatccgcaca 
     7501 tgaggcggaa ggtttccagc ttgagcgggt acggctcccg gtgcgagctg aaatagtcga 
     7561 acatccgtcg ggccgtcggc gacagcttgc ggtacttctc ccatatgaat ttcgtgtagt 
     7621 ggtcgccagc aaacagcacg acgatttcct cgtcgatcag gacctggcaa cgggacgttt 
     7681 tcttgccacg gtccaggacg cggaagcggt gcagcagcga caccgattcc aggtgcccaa 
     7741 cgcggtcgga cgtgaagccc atcgccgtcg cctgtaggcg cgacaggcat tcctcggcct 
     7801 tcgtgtaata ccggccattg atcgaccagc ccaggtcctg gcaaagctcg tagaacgtga 
     7861 aggtgatcgg ctcgccgata ggggtgcgct tcgcgtactc caacacctgc tgccacacca 
     7921 gttcgtcatc gtcggcccgc agctcgacgc cggtgtaggt gatcttcacg tccttgttga 
     7981 cgtggaaaat gaccttgttt tgcagcgcct cgcgcgggat tttcttgttg cgcgtggtga 
     8041 acagggcaga gcgggccgtg tcgtttggca tcgctcgcat cgtgtccggc cacggcgcaa 
     8101 tatcgaacaa ggaaagctgc atttccttga tctgctgctt cgtgtgtttc agcaacgcgg 
     8161 cctgcttggc ctcgctgacc tgttttgcca ggtcctcgcc ggcggttttt cgcttcttgg 
     8221 tcgtcatagt tcctcgcgtg tcgatggtca tcgacttcgc caaacctgcc gcctcctgtt 
     8281 cgagacgacg cgaacgctcc acggcggccg atggcgcggg cagggcaggg ggagccagtt 
     8341 gcacgctgtc gcgctcgatc ttggccgtag cttgctggac catcgagccg acggactgga 
     8401 aggtttcgcg gggcgcacgc atgacggtgc ggcttgcgat ggtttcggca tcctcggcgg 
     8461 aaaaccccgc gtcgatcagt tcttgcctgt atgccttccg gtcaaacgtc cgattcattc 
     8521 accctccttg cgggattgcc ccggaattaa ttccccggat cgatccgtcg atcttgatcc 
     8581 cctgcgccat cagatccttg gcggcaagaa agccatccag tttactttgc agggcttccc 
     8641 aaccttacca gagggcgccc cagctggcaa ttccggttcg cttgctgtcc ataaaaccgc 
     8701 ccagtctagc tatcgccatg taagcccact gcaagctacc tgctttctct ttgcgcttgc 
     8761 gttttccctt gtccagatag cccagtagct gacattcatc cggggtcagc accgtttctg 
     8821 cggactggct ttctacgtgg ctgccatttt tggggtgagg tcgttcgcgg ccgaggggcg 
     8881 cagcccctgg ggggatgggg tgccgcgtta gcgggccggg agggttcgag aagggggggc 
     8941 accccccttc ggcgtgcgcg gtcacgcgcc agggcgcagc cctggttaaa aacaaggttt 
     9001 ataaatattg gtttaaaagc aggttaaaag acaggttagc ggtggccgaa aaacgggcgg 
     9061 aaacccttgc aaatgctgga ttttctgcct gtggacagcc cctcaaatgt caataggtgc 
     9121 gcccctcatc tgtcatcact ctgcccctca agtgtcaagg atcgcgcccc tcatctgtca 
     9181 gtagtcgcgc ccctcaagtg tcaataccgc agggcactta tccccaggct tgtccacatc 
     9241 atctgtggga aactcgcgta aaatcaggcg ttttcgccga tttgcgaggc tggccagctc 
     9301 cacgtcgccg gccgaaatcg agcctgcccc tcatctgtca acgccgcgcc gggtgagtcg 
     9361 gcccctcaag tgtcaacgtc cgcccctcat ctgtcagtga gggccaagtt ttccgcgtgg 
     9421 tatccacaac gccggcggcc ctacatggct ctgctgtagt gagtgggttg cgctccggca 
     9481 gcggtcctga tcccccgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
     9541 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
     9601 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
     9661 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
     9721 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
     9781 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
     9841 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
     9901 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
     9961 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 
    10021 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
    10081 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
    10141 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
    10201 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
    10261 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg 
    10321 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
    10381 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
    10441 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
    10501 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
    10561 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
    10621 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctggg 
    10681 tcgatcgacg gatcttttcc gctgcataac cctgcttcgg ggtcattata gcgatttttt 
    10741 cggtatatcc atcctttttc gcacgatata caggattttg ccaaagggtt cgtgtagact 
    10801 ttccttggtg tatccaacgg cgtcagccgg gcaggatagg tgaagtaggc ccacccgcga 
    10861 gcgggtgttc cttcttcact gtcccttatt cgcacctggc ggtgctcaac gggaatcctg 
    10921 ctctgcgagg ctggccgtaa gctctaagaa accattatta tcatgacatt aacctataaa 
    10981 aataggcgta tcacgaggcc ctttcgtctt caagaattaa ttcactggcc gtcgttttac 
    11041 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc 
    11101 ctttcgccag caga 
//
