LOCUS       pSV23IVNAm12            5031 bp    DNA     circular     23-AUG-2018
DEFINITION  LMBP 3717.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pSV23IVNAm12|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
FEATURES             Location/Qualifiers
     CDS             1..809
                     /vntifkey="4"
                     /label=(VP1)
                     /note="part of the SV40 VP1 coding sequence"
     terminator      complement(810..906)
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 early region"
     CDS             complement(1011..2420)
                     /vntifkey="4"
                     /label=IVNA\mutant
                     /note="neuraminidase gene"
     promoter        complement(2517..2798)
                     /vntifkey="30"
                     /label=SV40E
                     /note="SV40 early promoter"
     rep_origin      complement(2485..2560)
                     /vntifkey="33"
                     /label=SVORI
                     /note="bidirectional origin of SV40"
     rep_origin      complement(3015..3425)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3963..4823)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     terminator      810..906
                     /vntifkey="43"
                     /label=polyA
                     /note="polyA signal of the SV40 late region"
     mutation        complement(2295..2297)
                     /vntifkey="62"
                     /label=C42S
                     /note="WT: TGC"
     mutation        complement(2292..2294)
                     /vntifkey="62"
                     /label=D43M
                     /note="WT: GAC"
     mutation        complement(2289..2291)
                     /vntifkey="62"
                     /label=S44A
                     /note="WT: TCC"
     mutation        complement(2187..2189)
                     /vntifkey="62"
                     /label=C78R
                     /note="WT: TGC"
     mutation        complement(2163..2165)
                     /vntifkey="62"
                     /label=N86Q
                     /note="WT: AAT"
BASE COUNT     1313 a      1172 c      1152 g      1369 t        25 others
ORIGIN
        1 ttcctttgcc taatttaaat gaggacttaa cctgtggaaa tattttgatg tgggaagctg 
       61 ttactgttaa aactgaggtt attggggtaa ctgctatgtt aaacttgcat tcagggacac 
      121 aaaaaactca tgaaaatggt gctggaaaac ccattcaagg gtcaaatttt catttttttg 
      181 ctgttggtgg ggaacctttg gagctgcagg gtgtgttagc aaactacagg accaaatatc 
      241 ctgctcaaac tgtaacccca aaaaatgcta cagttgacag tcagcagatg aacactgacc 
      301 acaaggctgt tttggataag gataatgctt atccagtgga gtgctgggtt cctgatccaa 
      361 gtaaaaatga aaacactaga tattttggaa cctacacagg tggggaaaat gtgcctcctg 
      421 ttttgcacat tactaacaca gcaaccacag tgcttcttga tgagcagggt gttgggccct 
      481 tgtgcaaagc tgacagcttg tatgtttctg ctgttgacat ttgtgggctg tttaccaaca 
      541 cttctggaac acagcagtgg aagggacttc ccagatattt taaaattacc cttagaaagc 
      601 ggtctgtgaa aaacccctac ccaatttcct ttttgttaag tgacctaatt aacaggagga 
      661 cacagagggt ggatgggcag cctatgattg gaatgtcctc tcaagtagag gaggttaggg 
      721 tttatgagga cacagaggag cttcctgggg atccagacat gataagatac attgatgagt 
      781 ttggacaaac cacaactaga atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg 
      841 ctattgcttt atttgtaacc attataagct gcaataaaca agttaacaac aacaattgca 
      901 ttcattttat gtttcaggtt cagggggagg tgtgggaggt tttttaaagc aagtaaaacc 
      961 tctacaaatg tggtatggct gattatgatc cgtcgactct agagccaagc ttatataggc 
     1021 atgagattga tgtccgcccc atcaggccat gagcctgtcc cataggtacc tgaagtgcca 
     1081 caaaacacaa caatactgtt tgaggtccac catactctag tttcctgttc ccttcccctt 
     1141 atcaactcca cataaaagca cctattgatg cagcttttgc cctcaacaga gaaaatacca 
     1201 gaataacctg acctattagc gctgtcaact atgacttgcc tatttatctg caatttggaa 
     1261 ttaggtgtgg accaaccacc aatgactttg aaggtttcat aacctgagcg tgaatcctcg 
     1321 ctgatcgttc ttcccatcca cacgtcattt ccatcgtcaa aggcccagcc tttcactccg 
     1381 tgattccctt tttcattgtt aggattccgg caatagctac tgctagatct gtcgtttttt 
     1441 ctgggtgtgt cgccaacaag ccctgagcac acataactgg aatcaatgct ataatctttc 
     1501 acatttatat ctacgactgg cctattagag cctttccagt tgtctctgca gatacatctg 
     1561 acaccaggat atcgaggata acaggaacac tcctctacat gctgagcact tcctgacaat 
     1621 gggctaatat gaacaatttt cccctcttca atgaatagta ttttagtatc agctcttcct 
     1681 gaagcacttc catcagtcat tactactgta caagtcccat tgatacaaac acattccgac 
     1741 tcctgggtcc tgaggatatt ttgagaccat gaaccaatgc tgtctacaag cctcccatcg 
     1801 taaatgaagc tagcagttgc atttttatca tacccagtga cacaaacatg cagccatgct 
     1861 tttccatcgt gacaacttga gctggaccat gctatacaca cttgcctggt tcccaagtga 
     1921 aatggaacac ccaactcatt catcaatagg gttcgatgag gggttctatc atgtattgtg 
     1981 tcatttgaat gtttgttttc tagtgtggtc ccctgcccga gtgcaaattg ataacatttg 
     2041 ccaggatcgc atgacacata aggttctctc gtcacccaaa tgtccccacc agcagaaagc 
     2101 cgaattgaat tgtccttaga gaaaggtgca aatcctgtaa ttttacattg tggctttgac 
     2161 cattgcctgt attccactaa tttaggcctt atctcttttt ctatggtggt gttagtcaaa 
     2221 tacactatct ctgttatgtt cctttctatt attattggtt cacacggcat tacttggttg 
     2281 ttcgcggggg ccatggactc atattgcttg aaatgcaatg ttacagtagt taccaggatg 
     2341 gcaatctgca tgaggaagca tattgttgca atggtgagag agacagagcc aattgttatt 
     2401 atcttttgat ttggattcat nnnnnnnnnn nnnnnnnnnn nnnnnccaag cttgagcttt 
     2461 ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag ctcagaggcc 
     2521 gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg cggagaatgg 
     2581 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct 
     2641 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca 
     2701 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct 
     2761 ggggactttc cacaccctaa ctgacacaca ttccacagtc gggcagcgtt gggtcctggc 
     2821 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc 
     2881 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca 
     2941 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc 
     3001 tggaaacgcg gaagtcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 
     3061 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 
     3121 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 
     3181 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 
     3241 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 
     3301 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 
     3361 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 
     3421 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 
     3481 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 
     3541 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 
     3601 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 
     3661 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 
     3721 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 
     3781 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 
     3841 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 
     3901 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 
     3961 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 
     4021 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 
     4081 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 
     4141 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 
     4201 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 
     4261 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 
     4321 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 
     4381 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 
     4441 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 
     4501 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 
     4561 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 
     4621 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 
     4681 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 
     4741 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 
     4801 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 
     4861 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 
     4921 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 
     4981 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a 
//
