ID pSEAPEhBCTNf1 standard; circular DNA; ; 6210 BP. DE LMBP 3787. CC Accession number: LMBP 3787 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 18-JUL-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 60..162 FT /vntifkey="29" FT /label=human\BCTN\promoter\region FT /note="human ß-catenin promoter region" FT misc_feature 163..201 FT /vntifkey="21" FT /label=5'\UTR\of\human\BCTN FT /note="part of exon 1 (non-coding) of the human ß-catenin gene" FT CDS 212..1732 FT /vntifkey="4" FT /label=human\SEAP FT /note="signal (212-265) and mature sequence" FT CDS 2248..2322 FT /vntifkey="4" FT /label=part\of\SV40\small\t-antigen FT /note="part of the SV40 small t-antigen" FT intron 2323..2389 FT /vntifkey="15" FT /label=intron\SV40\small\t-antigen FT /note="intron of the SV40 small t-antigen" FT misc_feature 2390..2941 FT /vntifkey="21" FT /label=3'\UTR\SV40\small\t-antigen FT /note="3' UTR of the SV40 small t-antigen" FT terminator 2942..3038 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(2942..3038) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(3484..3886) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(4427..5287) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT rep_origin 5419..5874 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT terminator 6042..6138 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(6042..6138) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT promoter complement(3103..3338) FT /vntifkey="29" FT /label=SV40\early\promoter\fragment\(enhancer) SQ Sequence 6210 BP; 1525 A; 1600 C; 1573 G; 1512 t; cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc taattcgtcg 60 ccccttgtcc tcgcgcggcg gaacgctccg cgctgcgccg gtggcggcag gatacagcgg 120 cttctgcgcg acttataaga gctccttgtg cggcgccatt ttaagcctct cggtctgtgg 180 cagcagcgtt ggcccggccc cagcttcctg catgctgctg ctgctgctgc tgctgggcct 240 gaggctacag ctctccctgg gcatcatccc agttgaggag gagaacccgg acttctggaa 300 ccgcgaggca gccgaggccc tgggtgccgc caagaagctg cagcctgcac agacagccgc 360 caagaacctc atcatcttcc tgggcgatgg gatgggggtg tctacggtga cagctgccag 420 gatcctaaaa gggcagaaga aggacaaact ggggcctgag atacccctgg ccatggaccg 480 cttcccatat gtggctctgt ccaagacata caatgtagac aaacatgtgc cagacagtgg 540 agccacagcc acggcctacc tgtgcggggt caagggcaac ttccagacca ttggcttgag 600 tgcagccgcc cgctttaacc agtgcaacac gacacgcggc aacgaggtca tctccgtgat 660 gaatcgggcc aagaaagcag ggaagtcagt gggagtggta accaccacac gagtgcagca 720 cgcctcgcca gccggcacct acgcccacac ggtgaaccgc aactggtact cggacgccga 780 cgtgcctgcc tcggcccgcc aggaggggtg ccaggacatc gctacgcagc tcatctccaa 840 catggacatt gacgtgatcc taggtggagg ccgaaagtac atgtttcgca tgggaacccc 900 agaccctgag tacccagatg actacagcca aggtgggacc aggctggacg ggaagaatct 960 ggtgcaggaa tggctggcga agcgccaggg tgcccggtat gtgtggaacc gcactgagct 1020 catgcaggct tccctggacc cgtctgtgac ccatctcatg ggtctctttg agcctggaga 1080 catgaaatac gagatccacc gagactccac actggacccc tccctgatgg agatgacaga 1140 ggctgccctg cgcctgctga gcaggaaccc ccgcggcttc ttcctcttcg tggagggtgg 1200 tcgcatcgac catggtcatc atgaaagcag ggcttaccgg gcactgactg agacgatcat 1260 gttcgacgac gccattgaga gggcgggcca gctcaccagc gaggaggaca cgctgagcct 1320 cgtcactgcc gaccactccc acgtcttctc cttcggaggc taccccctgc gagggagctc 1380 catcttcggg ctggcccctg gcaaggcccg ggacaggaag gcctacacgg tcctcctata 1440 cggaaacggt ccaggctatg tgctcaagga cggcgcccgg ccggatgtta ccgagagcga 1500 gagcgggagc cccgagtatc ggcagcagtc agcagtgccc ctggacgaag agacccacgc 1560 aggcgaggac gtggcggtgt tcgcgcgcgg cccgcaggcg cacctggttc acggcgtgca 1620 ggagcagacc ttcatagcgc acgtcatggc cttcgccgcc tgcctggagc cctacaccgc 1680 ctgcgacctg gcgccccccg ccggcaccac cgacgccgcg cacccgggtt aacccgtggt 1740 ccccgcgttg cttcctctgc tggccgggac atcaggtggc ccccgctgaa ttggaatcga 1800 tattgttaca acaccccaac atcttcgacg cgggcgtggc aggtcttccc gacgatgacg 1860 ccggtgaact tcccgccgcc gttgttgttt tggagcacgg aaagacgatg acggaaaaag 1920 agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa aaagttgcgc ggaggagttg 1980 tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact cgacgcaaga aaaatcagag 2040 agatcctcat aaaggccaag aagggcggaa agtccaaatt gtaaaatgta actgtattca 2100 gcgatgacga aattcttagc tattgtaata ctgcgatgag tggcagggcg gggcgtaatt 2160 tttttaaggc agttattggt gcccttaaac gcctggtgct acgcctgaat aagtgataat 2220 aagcggatga atggcagaaa ttcgccggat ctttgtgaag gaaccttact tctgtggtgt 2280 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt 2340 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta 2400 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc 2460 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc 2520 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt 2580 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga 2640 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag 2700 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc 2760 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt 2820 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat 2880 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata 2940 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa 3000 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 3060 tgtccaaact catcaatgta tcttatcatg tctggatctg aaccatggag cggagaatgg 3120 gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac tatggttgct 3180 gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg ggactttcca 3240 cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc tggggagcct 3300 ggggactttc cacaccctaa ctgacacaca ttccacagca gatcctctag ggatccgtcg 3360 accgatgccc ttgagagcct tcaacccagt cagctccttc cggtgggcgc ggggcatgac 3420 tatcgtcgcc gcacttatga ctgtcttctt tatcatgcaa ctcgtaggac aggtgccggc 3480 agcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 3540 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 3600 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 3660 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 3720 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 3780 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 3840 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 3900 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 3960 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 4020 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 4080 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 4140 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 4200 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 4260 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 4320 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 4380 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 4440 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 4500 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 4560 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 4620 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 4680 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 4740 ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 4800 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 4860 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 4920 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 4980 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 5040 caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 5100 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 5160 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 5220 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 5280 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 5340 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 5400 cccgaaaagt gccacctgac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 5460 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 5520 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 5580 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 5640 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 5700 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 5760 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 5820 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttcccatt 5880 cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac 5940 gccagcccaa gctaccatga taagtaagta atattaaggt acgtggaggt tttacttgct 6000 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt 6060 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc 6120 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta 6180 tcttatggta ctgtaactga gctaacataa 6210 //