LOCUS       pBluhCASP-6             4292 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 3814.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pBluhCASP-6|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pBluhCASP-6" 0 4292 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1952670563 0 "" "" 0 0 0 0 (CObList) (CObList) 
COMMENT        (CObList) (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4292 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "f1ORI" 33 0 0 4 459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4..459") 
COMMENT        #4=(CFSignal (CObList) "T7" 30 0 0 623 639 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "623..639") 
COMMENT        #5=(CFSignal (CObList) "3UTR" 21 0 1 690 1139 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(690..1139)") 
COMMENT        #6=(CFSignal (CObList) "hCASP6" 4 0 1 1140 2021 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1140..2021)") 
COMMENT        #7=(CFSignal (CObList) "T3" 30 0 1 2106 2122 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2106..2122)") 
COMMENT        #8=(CFSignal (CObList) "lac" 30 0 1 2186 2269 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2186..2269)") 
COMMENT        #9=(CFSignal (CObList) "ORI" 33 0 1 2361 2763 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2361..2763)") 
COMMENT        #10=(CFSignal (CObList) "AMP" 4 0 1 3304 4164 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3304..4164)")) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                          "DNA 'pBluhCASP-6'" 1) 
COMMENT                     #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "Last Modification Date: ? (no data)" 1) 
COMMENT                     #18=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 4292 bp" 1) 
COMMENT                     #19=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                   "Annotations") 
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COMMENT                                        "Start: 1140  End: 2021 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #29=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "human caspase-6 coding sequence" 1) 
COMMENT                                   #30=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #31=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1140..2021)" 1))) 
COMMENT                                 "hCASP6") 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
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COMMENT                                        "3' UTR of the human caspase-6 gene" 1) 
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COMMENT                                        "   complement(690..1139)" 1))) "3UTR"))) 
COMMENT                          "Misc. Feature (1 total)") 
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COMMENT                                        "Start: 623   End: 639 " 1) 
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COMMENT                                        "phage T7 gene 10 promoter" 1) 
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COMMENT                                        "E. coli lac operon promoter" 1) 
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COMMENT                          "Promoter Prokaryotic (3 total)") 
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FEATURES             Location/Qualifiers
     rep_origin      4..459
                     /vntifkey="33"
                     /label=f1ORI
                     /note="phage f1 origin"
     promoter        623..639
                     /vntifkey="30"
                     /label=T7
                     /note="phage T7 gene 10 promoter"
     misc_feature    complement(690..1139)
                     /vntifkey="21"
                     /label=3UTR
                     /note="3' UTR of the human caspase-6 gene"
     CDS             complement(1140..2021)
                     /vntifkey="4"
                     /label=hCASP6
                     /note="human caspase-6 coding sequence"
     promoter        complement(2106..2122)
                     /vntifkey="30"
                     /label=T3
                     /note="phage T3 promoter"
     promoter        complement(2186..2269)
                     /vntifkey="30"
                     /label=lac
                     /note="E. coli lac operon promoter"
     rep_origin      complement(2361..2763)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3304..4164)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1034 a      1076 c      1014 g      1168 t 
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg 
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg 
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg 
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtaccgggcc 
      661 ccccctcgag tttttttttt ttttttttta aggcagttct ctgctaggca ttaaacttta 
      721 aaacatttga atcattagac cataatgctt caccctaacg atatttatat aaaaggaaga 
      781 gaaagacatt ttcttttttt ttgagacgga gtttcactcg ttgcccaggc tggagtgcaa 
      841 tggcgcaatg ctcggctcac cgcagcctcc acctcctggg ttcaagtgat tctcctgcct 
      901 cagccttcca agtagctggg attgcaggca tgcgccgcca tgcctagcta aatttttttt 
      961 tgcattttta gtagaggcgg ggcttctgcc atgttggtca ggctggtctc gaactcccga 
     1021 cctcaggtga tccgcccacc ttggactccc aaagtgctgg gattacaggt gtgagtaacc 
     1081 acgcctggct gagaaagcca ttttcaatac agagtgtgaa attagatagc ctctattaat 
     1141 taattagatt ttggaaagaa atgcagcttt ttagttagca ttgaggcaaa acagggaacc 
     1201 tgcttctttc caattgcact tgggtctttg caaaagtcca ctcggcgctg agaaactttc 
     1261 ctgttcacca gtgtgaggag ttctgtgaac tctaaggagg agccatattt tcccaacatc 
     1321 tcacacaaat cttgaatgta ccatgagccg ttcacagttt cccggtgaga ataatatcct 
     1381 tctgcaacag agtaacacat gaggaagtca gctccagcag gcagcgtgta aacggaggct 
     1441 gcatccacct cagttatgtt ggtgtccaac ttctctgtct gattatctac tacatccaaa 
     1501 ggaatgactg gcacatcgtg ctggtttccc cgacatgcct ggatgataaa tatcttgggt 
     1561 tttccaacca ggctgtgaca cttgtctcct ttgaacaagc cagttaatgt ctgaatttcg 
     1621 attttagcat catatgcata aatgtgattg ccttcgccat ggctcaggaa gacacacaca 
     1681 aagcaatcgg catctgcgtg gctaacagtt gacacctcat gaattttgag cagtagttct 
     1741 tctgctttaa gatcattaaa gcatttcact tcaaatccta gatctgaaaa cctgcgggta 
     1801 agattgtctc tatctgcgca ggtgcgccgc ctttctggca gtgttaagtg ccaaaagaac 
     1861 ctctcatgat tgaagattaa agcaattcct ctcctcctgt ggtccatttt gtacttttct 
     1921 gccggatcaa acatttctct tttatagaag gcatctgttt ctgtcatgtt ttcttcccca 
     1981 cctgccgggt gccccctgcg gagccccgag gccgagctca ttgcagctcg agaattcctg 
     2041 cagcccgggg gatccactag ttctagagcg gccgccaccg cggtggagct ccagcttttg 
     2101 ttccctttag tgagggttaa tttcgagctt ggcgtaatca tggtcatagc tgtttcctgt 
     2161 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 
     2221 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 
     2281 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 
     2341 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 
     2401 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 
     2461 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 
     2521 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 
     2581 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 
     2641 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 
     2701 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 
     2761 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 
     2821 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 
     2881 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 
     2941 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 
     3001 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 
     3061 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 
     3121 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 
     3181 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 
     3241 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 
     3301 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 
     3361 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 
     3421 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 
     3481 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 
     3541 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 
     3601 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 
     3661 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 
     3721 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 
     3781 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 
     3841 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 
     3901 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 
     3961 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 
     4021 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 
     4081 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 
     4141 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 
     4201 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 
     4261 ggttccgcgc acatttcccc gaaaagtgcc ac 
//
