LOCUS       p425GAL1    7534 bp dsDNA    Circular
DEFINITION  LMBP 4025.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      complement(6124..7181)
                     /label=2mu\ori
     CDS             complement(663..1769)
                     /label=LEU2
     CDS             complement(5132..5992)
                     /label=AMP
     promoter        3100..3116
                     /label=T7\promoter
     terminator      complement(3137..3384)
                     /label=CYC1\terminator
     promoter        complement(3515..3898)
                     /label=GAL1\promoter
     promoter        complement(3934..3950)
                     /label=T3\promoter
     promoter        complement(4014..4097)
                     /label=lac\promoter
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 
      241 accattatgg gaaatgcttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 
     1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata 
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg 
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 
     3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 
     3121 gaattgggta ccggccgcaa attaaagcct tcgagcgtcc caaaaccttc tcaagcaagg 
     3181 ttttcagtat aatgttacat gcgtacacgc gtctgtacag aaaaaaaaga aaaatttgaa 
     3241 atataaataa cgttcttaat actaacataa ctataaaaaa ataaataggg acctagactt 
     3301 caggttgtct aactccttcc ttttcggtta gagcggatgt ggggggaggg cgtgaatgta 
     3361 agcgtgacat aactaattac atgactcgag gtcgacggta tcgataagct tgatatcgaa 
     3421 ttcctgcagc ccgggggatc cactagttct agatatagtt ttttctcctt gacgttaaag 
     3481 tatagaggta tattaacaat tttttgttga tacttttatt acatttgaat aagaagtaat 
     3541 acaaaccgaa aatgttgaaa gtattagtta aagtggttat gcagtttttg catttatata 
     3601 tctgttaata gatcaaaaat catcgcttcg ctgattaatt accccagaaa taaggctaaa 
     3661 aaactaatcg cattatcatc ctatggttgt taatttgatt cgttcatttg aaggtttgtg 
     3721 gggccaggtt actgccaatt tttcctcttc ataaccataa aagctagtat tgtagaatct 
     3781 ttattgttcg gagcagtgcg gcgcgaggca catctgcgtt tcaggaacgc gaccggtgaa 
     3841 gacgaggacg cacggaggag agtcttcctt cggagggctg tcacccgctc ggcggcttct 
     3901 aatccgtact tcagagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg 
     3961 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 
     4021 acataggagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca 
     4081 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 
     4141 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 
     4201 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     4261 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     4321 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     4381 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     4441 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     4501 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     4561 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     4621 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     4681 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     4741 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     4801 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     4861 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     4921 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     4981 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     5041 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     5101 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     5161 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     5221 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 
     5281 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 
     5341 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 
     5401 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 
     5461 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 
     5521 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 
     5581 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 
     5641 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 
     5701 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 
     5761 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 
     5821 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 
     5881 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 
     5941 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 
     6001 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 
     6061 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 
     6121 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt 
     6181 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta 
     6241 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc 
     6301 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag 
     6361 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag 
     6421 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata 
     6481 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt 
     6541 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta 
     6601 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc 
     6661 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt 
     6721 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt 
     6781 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct 
     6841 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca 
     6901 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag 
     6961 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca 
     7021 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag 
     7081 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg 
     7141 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt 
     7201 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata 
     7261 gtgcgtgttt atgcttaaat gcgtacttat atgcgtctat ttatgtagga tgaaaggtag 
     7321 tctagtacct cctgtgatat tatcccattc catgcggggt atcgtatgct tccttcagca 
     7381 ctacccttta gctgttctat atgctgccac tcctcaattg gattagtctc atccttcaat 
     7441 gctatcattt cctttgatat tggatcatac taagaaacca ttattatcat gacattaacc 
     7501 tataaaaata ggcgtatcac gaggcccttt cgtc 
//
