LOCUS       pRS426                  5726 bp    DNA     circular      6-AUG-2018
DEFINITION  LMBP 4064.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4064
            (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium)
            Last modified: 06-AUG-2018
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12403323|
COMMENT     VNTDBDATE|-12409176|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pRS426|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be/|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Yeast
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT         "NotI" "PstI" "PvuI" "SacI" "SacII" "SalI" "SpeI" "XhoI" "XmaI") 
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COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2301 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 2338 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SacI" "gagctc" 6 0 1 2359 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SacII" "ccgcgg" 5 0 1 2350 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 2274 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SpeI" "actagt" 2 0 1 2325 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 2268 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 2313 0 0 "") 
COMMENT        #10=(CRSite (CStringList) "BstXI" "ccannnnnntgg" 9 0 1 2351 0 0 "") 
COMMENT        #11=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 2289 0 0 "") 
COMMENT        #12=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 2257 0 0 "") 
COMMENT        #13=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 2 854 0 2297 0 0 "") 
COMMENT        #14=(CRSite (CStringList) "PstI" "ctgcag" 6 0 2 648 0 2311 0 0 "") 
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COMMENT        #21=(CFSignal (CObList) "lac promoter" 30 0 1 2454 2537 0 (CStringList) 
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COMMENT                          "DNA Plasmid Phage 'pRS426'" 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 08/02/18 09:18" 1) 
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COMMENT                          "length: 5726 bp" 1) 
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COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(2056..2415)
                     /vntifkey="4"
                     /label=lacZ\fragment,\incl.\lacZalpha
     rep_origin      complement(4564..5544)
                     /vntifkey="33"
                     /label=2\mu\ori
     rep_origin      complement(1599..2054)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        2225..2241
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(2374..2390)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(2454..2537)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(2416..2453)
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     rep_origin      complement(2629..3031)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(3572..4432)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     terminator      complement(5622..5726)
                     /vntifkey="43"
                     /label=FLP\terminator
     CDS             665..1468
                     /vntifkey="4"
                     /label=URA3
BASE COUNT     1568 a      1246 c      1370 g      1542 t 
ORIGIN
        1 ttatatgcgt ctatttatgt aggatgaaag gtagtctagt acctcctgtg atattatccc 
       61 attccatgcg gggtatcgta tgcttccttc agcactaccc tttagctgtt ctatatgctg 
      121 ccactcctca attggattag tctcatcctt caatgctatc atttcctttg atattggatc 
      181 atactaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc 
      241 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 
      301 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 
      361 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact 
      421 gagagtgcac cataccacag cttttcaatt caattcatca tttttttttt attctttttt 
      481 ttgatttcgg tttctttgaa atttttttga ttcggtaatc tccgaacaga aggaagaacg 
      541 aaggaaggag cacagactta gattggtata tatacgcata tgtagtgttg aagaaacatg 
      601 aaattgccca gtattcttaa cccaactgca cagaacaaaa acctgcagga aacgaagata 
      661 aatcatgtcg aaagctacat ataaggaacg tgctgctact catcctagtc ctgttgctgc 
      721 caagctattt aatatcatgc acgaaaagca aacaaacttg tgtgcttcat tggatgttcg 
      781 taccaccaag gaattactgg agttagttga agcattaggt cccaaaattt gtttactaaa 
      841 aacacatgtg gatatcttga ctgatttttc catggagggc acagttaagc cgctaaaggc 
      901 attatccgcc aagtacaatt ttttactctt cgaagacaga aaatttgctg acattggtaa 
      961 tacagtcaaa ttgcagtact ctgcgggtgt atacagaata gcagaatggg cagacattac 
     1021 gaatgcacac ggtgtggtgg gcccaggtat tgttagcggt ttgaagcagg cggcagaaga 
     1081 agtaacaaag gaacctagag gccttttgat gttagcagaa ttgtcatgca agggctccct 
     1141 atctactgga gaatatacta agggtactgt tgacattgcg aagagcgaca aagattttgt 
     1201 tatcggcttt attgctcaaa gagacatggg tggaagagat gaaggttacg attggttgat 
     1261 tatgacaccc ggtgtgggtt tagatgacaa gggagacgca ttgggtcaac agtatagaac 
     1321 cgtggatgat gtggtctcta caggatctga cattattatt gttggaagag gactatttgc 
     1381 aaagggaagg gatgctaagg tagagggtga acgttacaga aaagcaggct gggaagcata 
     1441 tttgagaaga tgcggccagc aaaactaaaa aactgtatta taagtaaatg catgtatact 
     1501 aaactcacaa attagagctt caatttaatt atatcagtta ttaccctatg cggtgtgaaa 
     1561 taccgcacag atgcgtaagg agaaaatacc gcatcaggaa attgtaaacg ttaatatttt 
     1621 gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat 
     1681 cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt 
     1741 ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt 
     1801 ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag 
     1861 gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg 
     1921 aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc 
     1981 gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc 
     2041 gctacagggc gcgtcgcgcc attcgccatt caggctgcgc aactgttggg aagggcgatc 
     2101 ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt 
     2161 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgagcg 
     2221 cgcgtaatac gactcactat agggcgaatt gggtaccggg ccccccctcg aggtcgacgg 
     2281 tatcgataag cttgatatcg aattcctgca gcccggggga tccactagtt ctagagcggc 
     2341 cgccaccgcg gtggagctcc agcttttgtt ccctttagtg agggttaatt gcgcgcttgg 
     2401 cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca 
     2461 acataggagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg aggtaactca 
     2521 cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc 
     2581 attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt 
     2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 
     2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 
     2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     3481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     3541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     3601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     3661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 
     3721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 
     3781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 
     3841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 
     3901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 
     3961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 
     4021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 
     4081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 
     4141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 
     4201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 
     4261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 
     4321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 
     4381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 
     4441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 
     4501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 
     4561 ctgaacgaag catctgtgct tcattttgta gaacaaaaat gcaacgcgag agcgctaatt 
     4621 tttcaaacaa agaatctgag ctgcattttt acagaacaga aatgcaacgc gaaagcgcta 
     4681 ttttaccaac gaagaatctg tgcttcattt ttgtaaaaca aaaatgcaac gcgagagcgc 
     4741 taatttttca aacaaagaat ctgagctgca tttttacaga acagaaatgc aacgcgagag 
     4801 cgctatttta ccaacaaaga atctatactt cttttttgtt ctacaaaaat gcatcccgag 
     4861 agcgctattt ttctaacaaa gcatcttaga ttactttttt tctcctttgt gcgctctata 
     4921 atgcagtctc ttgataactt tttgcactgt aggtccgtta aggttagaag aaggctactt 
     4981 tggtgtctat tttctcttcc ataaaaaaag cctgactcca cttcccgcgt ttactgatta 
     5041 ctagcgaagc tgcgggtgca ttttttcaag ataaaggcat ccccgattat attctatacc 
     5101 gatgtggatt gcgcatactt tgtgaacaga aagtgatagc gttgatgatt cttcattggt 
     5161 cagaaaatta tgaacggttt cttctatttt gtctctatat actacgtata ggaaatgttt 
     5221 acattttcgt attgttttcg attcactcta tgaatagttc ttactacaat ttttttgtct 
     5281 aaagagtaat actagagata aacataaaaa atgtagaggt cgagtttaga tgcaagttca 
     5341 aggagcgaaa ggtggatggg taggttatat agggatatag cacagagata tatagcaaag 
     5401 agatactttt gagcaatgtt tgtggaagcg gtattcgcaa tattttagta gctcgttaca 
     5461 gtccggtgcg tttttggttt tttgaaagtg cgtcttcaga gcgcttttgg ttttcaaaag 
     5521 cgctctgaag ttcctatact ttctagagaa taggaacttc ggaataggaa cttcaaagcg 
     5581 tttccgaaaa cgagcgcttc cgaaaatgca acgcgagctg cgcacataca gctcactgtt 
     5641 cacgtcgcac ctatatctgc gtgttgcctg tatatatata tacatgagaa gaacggcata 
     5701 gtgcgtgttt atgcttaaat gcgtac 
//
