LOCUS       YEplac181    5741 bp dsDNA    Circular
DEFINITION  LMBP 4073.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             3940..4800
                     /label=ampR
     CDS             complement(2227..3319)
                     /label=LEU2
     CDS             216..500
                     /label=LACZ'
     rep_origin      743..2088
                     /label=2\micron
     rep_origin      5553..5564
                     /label=pMB1\ori
     promoter        151..171
                     /label=lac\promoter
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca 
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct 
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat 
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc 
      241 atgcctgcag gtcgactcta gaggatcccc gggtaccgag ctcgaattca ctggccgtcg 
      301 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac 
      361 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac 
      421 agttgcgcag cctgaatggc gaatggcgcc tgatgcggta ttttctcctt acgcatctgt 
      481 gcggtatttc acaccgcata tatcggatcg tacttgttac ccatcattga attttgaaca 
      541 tccgaacctg ggagttttcc ctgaaacaga tagtatattt gaacctgtat aataatatat 
      601 agtctagcgc tttacggaag acaatgtatg tatttcggtt cctggagaaa ctattgcatc 
      661 tattgcatag gtaatcttgc acgtcgcatc cccggttcat tttctgcgtt tccatcttgc 
      721 acttcaatag catatctttg ttaacgaagc atctgtgctt cattttgtag aacaaaaatg 
      781 caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa 
      841 atgcaacgcg aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa 
      901 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa 
      961 cagaaatgca acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc 
     1021 tacaaaaatg catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt 
     1081 ctcctttgtg cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa 
     1141 ggttagaaga aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac 
     1201 ttcccgcgtt tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc 
     1261 cccgattata ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg 
     1321 ttgatgattc ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata 
     1381 ctacgtatag gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct 
     1441 tactacaatt tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc 
     1501 gagtttagat gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc 
     1561 acagagatat atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat 
     1621 attttagtag ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag 
     1681 cgcttttggt tttcaaaagc gctctgaagt tcctatactt tctagctaga gaataggaac 
     1741 ttcggaatag gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag 
     1801 ctgcgcacat acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat 
     1861 atatacatga gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc 
     1921 tatttatgta ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg 
     1981 ggtatcgtat gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa 
     2041 ttggattagt ctcatccttc aatgctatca tttcctttga tattggatcg atccgatgat 
     2101 aagctgtcaa acatgagaat taattctacc ctatgaacat attccatttt gtaatttcgt 
     2161 gtcgtttcta ttatgaattt catttataaa gtttatgtac aaatatcata aaaaaagaga 
     2221 atctttttaa gcaaggattt tcttaacttc ttcggcgaca gcatcaccga cttcggtggt 
     2281 actgttggaa ccacctaaat caccagttct gatacctgca tccaaaacct ttttaactgc 
     2341 atcttcaatg gccttacctt cttcaggcaa gttcaatgac aatttcaaca tcattgcagc 
     2401 agacaagata gtggcgatag ggtcaacctt attctttggc aaatctggag cagaaccgtg 
     2461 gcatggttcg tacaaaccaa atgcggtgtt cttgtctggc aaagaggcca aggacgcaga 
     2521 tggcaacaaa cccaaggaac ctgggataac ggaggcttca tcggagatga tatcaccaaa 
     2581 catgttgctg gtgattataa taccatttag gtgggttggg ttcttaacta ggatcatggc 
     2641 ggcagaatca atcaattgat gttgaacctt caatgtagga aattcgttct tgatggtttc 
     2701 ctccacagtt tttctccata atcttgaaga ggccaaaaca ttagctttat ccaaggacca 
     2761 aataggcaat ggtggctcat gttgtagggc catgaaagcg gccattcttg tgattctttg 
     2821 cacttctgga acggtgtatt gttcactatc ccaagcgaca ccatcaccat cgtcttcctt 
     2881 tctcttacca aagtaaatac ctcccactaa ttctctgaca acaacgaagt cagtaccttt 
     2941 agcaaattgt ggcttgattg gagataagtc taaaagagag tcggatgcaa agttacatgg 
     3001 tcttaagttg gcgtacaatt gaagttcttt acggattttt agtaaacctt gttcaggtct 
     3061 aacactacct gtaccccatt taggaccacc cacagcacct aacaaaacgg catcaacctt 
     3121 cttggaggct tccagcgcct catctggaag tgggacacct gtagcatcga tagcagcacc 
     3181 accaattaaa tgattttcga aatcgaactt gacattggaa cgaacatcag aaatagcttt 
     3241 aagaacctta atggcttcgg ctgtgatctt gaccaacgtg gtcacctggc aaaacgacga 
     3301 tcttcttagg ggcagacata ggggcagaca ttagaatgta tatccttgaa atatatatat 
     3361 atattgctga aatgtaaaag gtaagaaaag ttagaataag acgattgcta accacctatt 
     3421 ggaaaaaaca ataggtcctt aaataatatt gtcaacttca agtattgtga tgcaagcatt 
     3481 tagtcatgaa cgcttctcta ttctatatga aaagccggtt ccggcctctc acctttcctt 
     3541 tttctcccaa tttttcagtt gaaaaaggta tatgcgcggc gacctctgaa attaacaaaa 
     3601 aatttccagt catcgaattt gtctgtgcga tagcgcccct gtgtgttctc gttatgttga 
     3661 ggaaaaaaat aatggttgct aagagattcg aactcttgca tcttacgata cctgagtatt 
     3721 cccacagtta attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat 
     3781 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga 
     3841 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa 
     3901 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt 
     3961 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg 
     4021 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg 
     4081 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg 
     4141 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag 
     4201 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca 
     4261 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg 
     4321 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc 
     4381 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg 
     4441 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg 
     4501 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac 
     4561 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg 
     4621 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg 
     4681 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact 
     4741 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa 
     4801 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt 
     4861 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag 
     4921 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct 
     4981 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt 
     5041 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg 
     5101 cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct 
     5161 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc 
     5221 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg 
     5281 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa 
     5341 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg 
     5401 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg 
     5461 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga 
     5521 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt 
     5581 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct 
     5641 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga 
     5701 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag a 
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