ID pGAL1PNiST-1 standard; circular DNA; UNA; 7176 BP. DE LMBP 4099. CC Accession number: LMBP 4099 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 15-MAY-2012 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter complement(12..28) FT /vntifkey="30" FT /label=SP6\promoter FT /note="Phage SP6 promoter" FT promoter complement(89..172) FT /vntifkey="30" FT /label=lac\promoter FT /note="Escherichia coli lac operon promoter" FT rep_origin complement(264..666) FT /vntifkey="33" FT /label=pMB1\ori FT CDS complement(1207..2067) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT rep_origin complement(2506..2961) FT /vntifkey="33" FT /label=f1\ori FT /note="Phage f1 origin" FT promoter 3125..3141 FT /vntifkey="30" FT /label=T7\promoter FT /note="Phage T7 gene 10 promoter (T7g10)" FT CDS 3692..4504 FT /vntifkey="4" FT /label=Candida\URA3 FT /note="C. albicans uracil 3 coding sequence" FT promoter 4799..6011 FT /vntifkey="29" FT /label=CaGAL1\promoter FT /note="C. albicans galactokinase 1 promoter" FT misc_feature complement(6069..6268) FT /vntifkey="21" FT /label=3'\UTR\of\hIFNß FT /note="3' UTR of the human interferon ß gene" FT CDS complement(6269..6832) FT /vntifkey="4" FT /label=hIFNß FT /note="human interferon ß signal and mature sequence" FT terminator 6906..7176 FT /vntifkey="43" FT /label=CYC1\terminator FT /note="S. cerevisiae iso-1-cytochrome C terminator" SQ Sequence 7176 BP; 2084 A; 1436 C; 1600 G; 2056 t; cttgagtatt ctatagtgtc acctaaatag cttggcgtaa tcatggtcat agctgtttcc 60 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 120 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 180 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 240 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 300 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 360 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 420 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 480 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 540 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 600 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 660 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 720 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 780 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 840 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 900 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 960 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 1020 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 1080 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 1140 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 1200 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 1260 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 1320 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 1380 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 1440 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 1500 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc 1560 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 1620 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 1680 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 1740 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 1800 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa 1860 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 1920 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 1980 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 2040 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 2100 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 2160 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 2220 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 2280 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 2340 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 2400 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg 2460 tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgaaatt gtaaacgtta 2520 atattttgtt aaaattcgcg ttaaatattt gttaaatcag ctcatttttt aaccaatagg 2580 ccgaaatcgg caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg 2640 ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa 2700 aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgc 2760 ggtcgaggtg ccgtaaagct ctaaatcgga accctaaagg gagcccccga tttagagctt 2820 gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg 2880 ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta 2940 atgcgccgct acagggcgcg tccattcgcc attcaggctg cgcaactgtt gggaagggcg 3000 atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg 3060 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 3120 attgtaatac gactcactat agggcgaatt ggttttccaa tgatgagcac ttttaaagtt 3180 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc 3240 atacactatt ctcagaatga cttggttgag tactaatagg aattgatttg gatggtataa 3300 acggaaacaa aaaaaagagc tggtactact ttctttaaaa ttattttatt atttgatttt 3360 atttaatagt atatattata ttttgaacgt agattatttt gttgaaagtt gctgtagtgc 3420 cattgattcg taacactaat tctgtattag tcattcctct tgtttgatag tatccaaaaa 3480 aacggctatt tttttgcaat cttatttcct gcatattata cagataacat aatgaaagaa 3540 aaaatctttt tttttgttct tcaatgatga tttcaaccat tcttttaaac attgatcaat 3600 tcctgagcaa caaccccata cacactggtt tatatatcgc cccttttaca gttgaagaaa 3660 gaaatagaaa tagaaatagc aaacaaaaga tatgacagtc aacactaaga cctatagtga 3720 gagagcagaa actcatgcct caccagtagc acaacgatta tttcgattaa tggaactgaa 3780 gaaaaccaat ttatgtgcat caattgatgt tgataccact aaggaattcc ttgaattaat 3840 tgataaattg ggtccttatg tatgcttaat caagactcat attgatataa tcaatgattt 3900 ttcctatgaa tccactattg aaccattatt agaactttca cgtaaacatc aatttatgat 3960 ttttgaagat agaaaatttg ctgatattgg taataccgtg aagaaacaat atattggtgg 4020 agtttataaa attagtagtt gggcagatat tactaatgct catggtgtca ctgggaatgg 4080 agtagttgaa ggattaaaac agggagctaa agaaaccacc accaaccaag agccaagagg 4140 gttattgatg ttagctgaat tatcatcagt gggatcatta gcatatggag aatattctca 4200 aaaaactgtt gaaattgcta aatccgataa ggaatttgtt attggattta ttgcccaacg 4260 tgatatgggt ggacaagaag aaggatttga ttggcttatt atgacacctg gagttggatt 4320 agatgataaa ggtgatggat taggacaaca atatagaact gttgatgaag ttgttagcac 4380 tggaactgat attatcattg ttggtagagg attgtttggt aaaggaagag atccagatat 4440 tgaaggtaaa aggtatagag atgctggttg gaatgcttat ttgaaaaaaa ctggccaatt 4500 ataaatgtga agggggagat tttcacttta ttagatttgt atatatgtat aataaataaa 4560 taaataagtt aaataaataa ttaaataagg gtggtaatta ttactattta caatcaaagg 4620 tggtccttct agctgtaatc cgggcagcgc aacggaacat tcatcagtgt aaaaatggaa 4680 tcaataaagc cctgcgcagc gcgcagggtc agcctgaata cgcgtttaat gaccagcaca 4740 gtcgtgatgg caaggtcaga atagcccaag tcggccgagg ggcctgtaca gtgagggaag 4800 atctgatatt gacgaagagg aaccaatgta acgttacact gaagaaaaca cacaataaac 4860 gggaagaaac ggtgtaaaag tgtgaaaata atttttgaat atcatttccc ttggtttaat 4920 tccaaacgaa acgtgttttt tttagagaat gggaattctt attggatgtc tagattgttt 4980 gtttactcca gactgtgcac aaaaacgttt ggatggatga tcagaagata tttttaggct 5040 tagctctaaa tataagaaat gatgcttgaa aaaccagaca gaaattgagt ttcaaaaatt 5100 ggtaatgtga ggtattagtc aactaaccaa ataacaatgc aaaccggttg atacatttca 5160 ttttgaaaat aatgaaactg gaattggatg accagcacac aaacacataa agtaattatg 5220 ggaattagaa gcgaacatag aggagtactt ggccacgaac agaatacaag tgggaacact 5280 attttctcca ttgttttagt tctgtttttt tgtcagccta gttttgtgct atgtgtaaaa 5340 aatattgcca agaaaaaaag cttgttttgt ggccagtgtc cgaaaaaaat tttggggaat 5400 cttcggatta atttatgttt tcattccatc ggggaaagtg ggggggaaaa aattttaagc 5460 agttcacaaa accttccaaa aaatatatgg acaaagatga ttgtattttc ccgacaccaa 5520 aatcataatt aattatgaga aagttaaatg taacgttaca atttatgttt atttgaaggt 5580 gaaaagcgat ttatgatttt tccgaaatga aaattttttt taggtttatt ttttttgtcg 5640 ggcaaagaaa aactgaacaa ggattattaa aatttttggt gtttgtttgt gtctggagaa 5700 ttcattcctc tctcatcttc acacaatgtt tagacatctg acacgattca tgatagttcg 5760 gtttccgggg ttggtgttta gttttcgttt ttcttttttt ttggaaagaa tgttttagct 5820 cattggtttt ctttcttcat tcaatagttt tgaaagaatt tgcccacttg ttattacaat 5880 catataaaat taaactttga tataaaatag agtttgaaag tttcccagat cctttttgat 5940 ttctttgtaa attttttttt ctcccacata tacacacata caaaccgatt tttataagaa 6000 agagttatac cctgcagctc gacctcgagg gatccgggcc ctctagatgc ggccgctagg 6060 cctcgaggga cttttgcacc aaaaataatt tattttccaa aataaaattt aaataaataa 6120 aaataactca taatttaata aaaatttcaa aatcttctag tgtcctttca tatgcagtac 6180 attagccatc agtcacttaa acagcatctg ctggttgaag aatgcttgaa gcaattgtcc 6240 agtcccagag gcacaggcta ggagatcttc agtttcggag gtaacctgta agtctgttaa 6300 tgaagtaaaa gttccttagg atttccactc tgactatggt ccaggcacag tgactgtact 6360 ccttggcctt caggtaatgc agaatcctcc cataatatct tttcaggtgc agactgctca 6420 tgagttttcc cctggtgaaa tcttctttct ccagtttttc ttccaggact gtcttcagat 6480 ggtttatctg atgatagaca ttagccagga ggttctcaac aatagtctca ttccagccag 6540 tgctagatga atcttgtctg aaaatagcaa agatgttctg gagcatctca tagatggtca 6600 atgcggcgtc ctccttctgg aactgctgca gctgcttaat ctcctcaggg atgtcaaagt 6660 tcatcctgtc cttgaggcag tattcaagcc tcccattcaa ttgccacagg agcttctgac 6720 actgaaaatt gctgcttctt tgtaggaatc caagcaagtt gtagctcatg gaaagagctg 6780 tagtggagaa gcacaacagg agagcaattt ggaggagaca cttgttggtc atgttcctcg 6840 aggccttttt ggccaagctg gcgcctgctg cgcgacggcg agctgctcac cacccaggat 6900 ccgtccccct tttcctttgt cgatatcatg taattagtta tgtcacgctt acattcacgc 6960 cctcccccca catccgctct aaccgaaaag gaaggagtta gacaacctga agtctaggtc 7020 cctatttatt tttttatagt tatgttagta ttaagaacgt tatttatatt tcaaattttt 7080 cttttttttc tgtacagacg cgtgtacgca tgtaacatta tactgaaaac cttgcttgag 7140 aaggttttgg gacgctcgaa ggctttaatt tgcaag 7176 //