LOCUS       pSG5-TIF2              10238 bp    DNA     circular     28-OCT-2013
DEFINITION  LMBP 4138.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4138
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 28-OCT-2013
COMMENT     VNTDATE|677065723|
COMMENT     VNTNAME|pSG5-TIF2|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

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FEATURES             Location/Qualifiers
     CDS             complement(8319..9179)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(7377..7778)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        28..309
                     /vntifkey="30"
                     /label=SV40\early\promoter
     intron          395..967
                     /vntifkey="15"
                     /label=intron\2\rabbit\ß-globin
     terminator      7234..7308
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      complement(9617..10072)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        1022..1038
                     /vntifkey="30"
                     /label=T7\promoter
     CDS             1202..5605
                     /vntifkey="4"
                     /label=hTIF2
     terminator      complement(7234..7308)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      266..341
                     /vntifkey="33"
                     /label=SV40\ori
     misc_feature    1049..1201
                     /vntifkey="21"
                     /label=5'\UTR\of\hTIF2
     misc_feature    5606..7204
                     /vntifkey="21"
                     /label=3'\UTR\of\hTIF2
BASE COUNT     2848 a      2461 c      2375 g      2554 t 
ORIGIN
        1 gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag 
       61 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc 
      121 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat 
      181 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt 
      241 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc 
      301 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt 
      361 tgcaaaaagc tggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc 
      421 tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt 
      481 gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt 
      541 tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac 
      601 tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt 
      661 gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata 
      721 ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt 
      781 tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct 
      841 ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat 
      901 aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt 
      961 cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt 
     1021 gtaatacgac tcactatagg gcgaattcgg cggccgcagc ctcggctaca gcttcggcgg 
     1081 cgaaggtcag cgccgacggc agccggcacc tgacggcgtg accgacccga gccgatttct 
     1141 cttggatttg gctacacact tatagatctt ctgcactgtt tacaggcaca gttgctgata 
     1201 tgtgttcaag atgagtggga tgggagaaaa tacctctgac ccctccaggg cagagacaag 
     1261 aaagcgcaag gaatgtcctg accaacttgg acccagcccc aaaaggaaca ctgaaaaacg 
     1321 taatcgtgaa caggaaaata aatatataga agaacttgca gagttgattt ttgcaaattt 
     1381 taatgatata gacaacttta acttcaaacc tgacaaatgt gcaatcttaa aagaaactgt 
     1441 gaagcaaatt cgtcagatca aagaacaaga gaaagcagca gctgccaaca tagatgaagt 
     1501 gcagaagtca gatgtatcct ctacagggca gggtgtcatc gacaaggatg cgctggggcc 
     1561 tatgatgctt gaggcccttg atgggttctt ctttgtagtg aacctggaag gcaacgttgt 
     1621 gtttgtgtca gagaatgtga cacagtatct aaggtataac caagaagagc tgatgaacaa 
     1681 aagtgtatat agcatcttgc atgttgggga ccacacggaa tttgtcaaaa acctgctgcc 
     1741 aaagtctata gtaaatgggg gatcttggtc tggcgaacct ccgaggcgga acagccatac 
     1801 cttcaattgt cggatgctgg taaaaccttt acctgattca gaagaggagg gtcatgataa 
     1861 ccaggaagct catcagaaat atgaaactat gcagtgcttc gctgtctctc aaccaaagtc 
     1921 catcaaagaa gaaggagaag atttgcagtc ctgcttgatt tgcgtggcaa gaagagttcc 
     1981 catgaaggaa agaccagttc ttccctcatc agaaagtttt actactcgcc aggatctcca 
     2041 aggcaagatc acgtctctgg ataccagcac catgagagca gccatgaaac caggctggga 
     2101 ggacctggta agaaggtgta ttcagaagtt ccatgcgcag catgaaggag aatctgtgtc 
     2161 ctatgctaag aggcatcatc atgaagtact gagacaagga ttggcattca gtcaaatcta 
     2221 tcgtttttcc ttgtctgatg gcactcttgt tgctgcacaa acgaagagca aactcatccg 
     2281 ttctcagact actaatgaac ctcaacttgt aatatcttta catatgcttc acagagagca 
     2341 gaatgtgtgt gtgatgaatc cggatctgac tggacaaacg atggggaagc cactgaatcc 
     2401 aattagctct aacagccctg cccatcaggc cctgtgcagt gggaacccag gtcaggacat 
     2461 gaccctcagt agcaatataa attttcccat aaatggccca aaggaacaaa tgggcatgcc 
     2521 catgggcagg tttggtggtt ctgggggaat gaaccatgtg tcaggcatgc aagcaaccac 
     2581 tcctcagggt agtaactatg cactcaaaat gaacagcccc tcacaaagca gccctggcat 
     2641 gaatccagga cagcccacct ccatgctttc accaaggcat cgcatgagcc ctggagtggc 
     2701 tggcagccct cgaatcccac ccagtcagtt ttcccctgca ggaagcttgc attcccctgt 
     2761 gggagtttgc agcagcacag gaaatagcca tagttatacc aacagctccc tcaatgcact 
     2821 tcaggccctc agcgaggggc acggggtctc attagggtca tcgttggctt caccagacct 
     2881 aaaaatgggc aatttgcaaa actccccagt taatatgaat cctcccccac tcagcaagat 
     2941 gggaagcttg gactcaaaag actgttttgg actatatggg gagccctctg aaggtacaac 
     3001 tggacaagca gagagcagct gccatcctgg agagcaaaag gaaacaaatg accccaacct 
     3061 gcccccggcc gtgagcagtg agagagctga cgggcagagc agactgcatg acagcaaagg 
     3121 gcagaccaaa ctcctgcagc tgctgaccac caaatctgat cagatggagc cctcgccctt 
     3181 agccagctct ttgtcggata caaacaaaga ctccacaggt agcttgcctg gttctgggtc 
     3241 tacacatgga acctcgctca aggagaagca taaaattttg cacagactct tgcaggacag 
     3301 cagttcccct gtggacttgg ccaagttaac agcagaagcc acaggcaaag acctgagcca 
     3361 ggagtccagc agcacagctc ctggatcaga agtgactatt aaacaagagc cggtgagccc 
     3421 caagaagaaa gagaatgcac tacttcgcta tttgctagat aaagatgata ctaaagatat 
     3481 tggtttacca gaaataaccc ccaaacttga gagactggac agtaagacag atcctgccag 
     3541 taacacaaaa ttaatagcaa tgaaaactga gaaggaggag atgagctttg agcctggtga 
     3601 ccagcctggc agtgagctgg acaacttgga ggagattttg gatgatttgc agaatagtca 
     3661 attaccacag cttttcccag acacgaggcc aggcgcccct gctggatcag ttgacaagca 
     3721 agccatcatc aatgacctca tgcaactcac agctgaaaac agccctgtca cacctgttgg 
     3781 agcccagaaa acagcactgc gaatttcaca gagcactttt aataacccac gaccagggca 
     3841 actgggcagg ttattgccaa accagaattt accacttgac atcacattgc aaagcccaac 
     3901 tggtgctgga cctttcccac caatcagaaa cagtagtccc tactcagtga tacctcagcc 
     3961 aggaatgatg ggtaatcaag ggatgatagg aaaccaagga aatttaggga acagtagcac 
     4021 aggaatgatt ggtaacagtg cttctcggcc tactatgcca tctggagaat gggcaccgca 
     4081 gagttcggct gtgagagtca cctgtgctgc taccaccagt gccatgaacc ggccagtcca 
     4141 aggaggtatg attcggaacc cagcagccag catccccatg aggcccagca gccagcctgg 
     4201 ccaaagacag acgcttcagt ctcaggtcat gaatataggg ccatctgaat tagagatgaa 
     4261 catgggggga cctcagtata gccaacaaca agctcctcca aatcagactg ccccatggcc 
     4321 tgaaagcatc ctgcctatag accaggcgtc ttttgccagc caaaacaggc agccatttgg 
     4381 cagttctcca gatgacttgc tatgtccaca tcctgcagct gagtctccga gtgatgaggg 
     4441 agctctcctg gaccagctgt atctggcctt gcggaatttt gatggcctgg aggagattga 
     4501 tagagcctta ggaatacccg aactggtcag ccagagccaa gcagtagatc cagaacagtt 
     4561 ctcaagtcag gattccaaca tcatgctgga gcagaaggcg cccgttttcc cacagcagta 
     4621 tgcatctcag gcacaaatgg cccagggtag ctattctccc atgcaagatc caaactttca 
     4681 caccatggga cagcggccta gttatgccac actccgtatg cagcccagac cgggcctcag 
     4741 gcccacgggc ctagtgcaga accagccaaa tcaactaaga cttcaacttc agcatcgcct 
     4801 ccaagcacag cagaatcgcc agccacttat gaatcaaatc agcaatgttt ccaatgtgaa 
     4861 cttgactctg aggcctggag taccaacaca ggcacctatt aatgcacaga tgctggccca 
     4921 gagacagagg gaaatcctga accagcatct tcgacagaga caaatgcatc agcaacagca 
     4981 agttcagcaa cgaactttga tgatgagagg acaagggttg aatatgacac caagcatggt 
     5041 ggctcctagt ggtatgccag caactatgag caaccctcgg attccccagg caaatgcaca 
     5101 gcagtttcca tttcctccaa actacggaat aagtcagcaa cctgatccag gctttactgg 
     5161 ggctacgact ccccagagcc cacttatgtc accccgaatg gcacatacac agagtcccat 
     5221 gatgcaacag tctcaggcca acccagccta tcaggccccc tccgacataa atggatgggc 
     5281 gcaggggaac atgggcggaa acagcatgtt ttcccagcag tccccaccac actttgggca 
     5341 gcaagcaaac accagcatgt acagtaacaa catgaacatc aatgtgtcca tggcgaccaa 
     5401 cacaggtggc atgagcagca tgaaccagat gacaggacag atcagcatga cctcagtgac 
     5461 ctccgtgcct acgtcagggc tgtcctccat gggtcccgag caggttaatg atcctgctct 
     5521 gaggggaggc aacctgttcc caaaccagct gcctggaatg gatatgatta agcaggaggg 
     5581 agacacaaca cggaaatatt gctgacactg ctgaagccag ttgcttcttc agctgaccgg 
     5641 gctcacttgc tcaaaacact tccagtctgg agagctgtgt ctatttgttt caacccaact 
     5701 gacctgccag ccggttctgc tagagcagac aggcctggcc ctggttccca gggtggcgtc 
     5761 cactcggctg tggcaggagg agctgcctct tctcttgaca gtctgaagct cgcatccaga 
     5821 cagtcgctca gtctgttccc tgcattcacc ttagtgcaac ttagatctct cctccccaag 
     5881 taaatgttga caggccaatt tcatacccat gtcagattga atgtatttaa atgtatgtat 
     5941 ttaaggagaa ccatgctctt gttctgttcc tgttcggttc cagacactgg tttcttgctt 
     6001 tgttttccct ggctaacagt ctagtgccaa agattaagat tttatctggg ggaaagaaaa 
     6061 gaatttttta aaaaattaaa ctaaagatgt tttaagctaa agcctgaatt tgggatggaa 
     6121 gcaggacaga caccgtggac agcgctgtat ttacagacac acccagtgcg tgaagaccaa 
     6181 caaagtcaca gtcgtatctc tagaaagctc taaagaccat gttggaaaga gtctccagtt 
     6241 actgaacaga tgaaaaggag cctgtgagag ggctgttaac attagcaaat attttttcct 
     6301 tgttttttct ttgttaaaac caaactggtt cacctgaatc atgaattgag aagaaataat 
     6361 tttcatttct aaattaagtc ccttttagtt tgatcagaca gcttgaatca gcatctcttc 
     6421 ttccctgtca gcctgactct tcccttcccc tctctcattc cccatactcc ctattttcat 
     6481 tcctttttta aaaaataata taagctacag aaaccaggta agccctttat ttccttaaat 
     6541 gttttgccag ccacttacca attgctaagt attgaatttc agaaaaaaaa aatgcattta 
     6601 ctggcaagga gaagagcaaa gttaaggctt gataccaatc gagctaagga tacctgcttt 
     6661 ggaagcatgt ttattctgtt ccccagcaac tctggcctcc aaaatgggag aaacgccagt 
     6721 gtgtttaaat tgatagcaga tatcacgaca gatttaacct ctgccatgtg ttttttattt 
     6781 tgttttttag cagtgctgac taagccgaag ttttgtaagg tacataaaat ccaatttata 
     6841 tgtaaacaag caataattta agttgagaac ttatgtgttt taattgtata atttttgtga 
     6901 ggtatacata ttgtggaatt gactcaaaaa tgaggtactt cagtattaaa ttagatatct 
     6961 tcatagcaat gtctcctaaa ggtgttttgt aaaggatatc aatgccttga ttagacctaa 
     7021 tttgtagact taagactttt tattttctaa accttgtgat tctgcttata agtcatttat 
     7081 ctaatctata tgatatgcag ccgctgtagg aaccaattct tgatttttat atgtttatat 
     7141 tctttcttaa tgaaccttag aaagactaca tgttactaag caggccactt ttatggttgt 
     7201 ttttgaattc ggatccagat cttattaaag cagaacttgt ttattgcagc ttataatggt 
     7261 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct 
     7321 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggtcgac tctagactct 
     7381 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca 
     7441 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac 
     7501 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt 
     7561 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg 
     7621 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc 
     7681 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc 
     7741 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc 
     7801 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac 
     7861 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt 
     7921 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct 
     7981 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc 
     8041 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt 
     8101 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg 
     8161 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc 
     8221 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa 
     8281 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag 
     8341 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg 
     8401 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga 
     8461 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag 
     8521 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa 
     8581 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc 
     8641 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca 
     8701 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg 
     8761 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat 
     8821 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc 
     8881 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg 
     8941 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg 
     9001 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt 
     9061 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca 
     9121 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata 
     9181 ctcttctttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac 
     9241 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa 
     9301 gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt 
     9361 atcacgaggc ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat 
     9421 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg 
     9481 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga 
     9541 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag 
     9601 aaaataccgc atcaggaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt 
     9661 tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca 
     9721 aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta 
     9781 aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta 
     9841 cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg 
     9901 aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga 
     9961 aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg 
    10021 ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcgcgccat 
    10081 tcgccattca ggctacgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 
    10141 cgccagctgg cgaagggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt 
    10201 tcccagtcac gacgttgtaa aacgacggcc agtgaatt 
//
