LOCUS       LNXCO3                  7484 bp    DNA     circular     19-APR-2010
DEFINITION  LMBP 4228.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4228
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 19-APR-2010
            
            
COMMENT     VNTNAME|LNXCO3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT        (CStringList "BamHI" "EcoRI" "NcoI" "NotI" "PvuI" "SpeI" "SspI" "XbaI" 
COMMENT         "NdeI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #2=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 2217 0 2831 0 3954 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 3149 0 6863 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SpeI" "actagt" 2 0 2 871 0 4315 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SspI" "aatatt" 4 0 3 3346 0 4419 0 7297 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 4828 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3140 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "NdeI" "catatg" 3 0 2 4082 0 5423 0 0 "")) 
COMMENT       (CObList 
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COMMENT        #10=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 1656 2450 0 
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COMMENT        #11=(CFSignal (CObList) "HSV-TK polyA" 43 0 1 3052 3121 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3052..3121)") 
COMMENT        #12=(CFSignal (CObList) "hCMV-IE promoter and enhancer" 29 0 1 3750 4333 
COMMENT             0 (CStringList) (CStringList) 1 1 1 1 "complement(3750..4333)") 
COMMENT        #13=(CFSignal (CObList) "CO3 unit" 21 0 1 3052 4333 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(2950..4333)") 
COMMENT        #14=(CFSignal (CObList) "3' MMLV-LTR" 43 0 0 4530 5123 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4530..5123") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 5476 5878 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5476..5882)") 
COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6419 7279 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6419..7279)") 
COMMENT        #17=(CFSignal (CObList) "SV40 small t intron containing 3 lac operators" 
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COMMENT             "complement(3185..3602)") 
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COMMENT        #20=(CFSignal (CObList) "LMBP 4228" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                                 "hCMV-IE promoter and enhancer"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                   "Restriction/Methylation Map")))) 
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FEATURES             Location/Qualifiers
     promoter        147..733
                     /vntifkey="29"
                     /label=5'\MMSV-LTR
     CDS             1656..2450
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      complement(3052..3121)
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     promoter        complement(3750..4333)
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     misc_feature    complement(3052..4333)
                     /vntifkey="21"
                     /label=CO3\unit
     terminator      4530..5123
                     /vntifkey="43"
                     /label=3'\MMLV-LTR
     rep_origin      complement(5476..5878)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(6419..7279)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     intron          complement(3185..3603)
                     /vntifkey="15"
                     /label=SV40\small\t\intron\containing\3\lac\operators
     misc_feature    803..1612
                     /vntifkey="21"
                     /label=extended\MMSV/MMLV\packaging\signal
                     /note="1133: end MMSV; 1134: start MMLV"
BASE COUNT     1743 a      2014 c      1923 g      1804 t 
ORIGIN
        1 gaattcatac cagatcaccg aaaactgtcc tccaaatgtg tccccctcac actcccaaat 
       61 tcgcgggctt ctgcctctta gaccactcta ccctattccc cacactcacc ggagccaaag 
      121 ccgcggccct tccgtttctt tgcttttgaa agaccccacc cgtaggtggc aagctagctt 
      181 aagtaacgcc actttgcaag gcatggaaaa atacataact gagaatagaa aagttcagat 
      241 caaggtcagg aacaaagaaa cagctgaata ccaaacagga tatctgtggt aagcggttcc 
      301 tgccccggct cagggccaag aacagatgag acagctgagt gatgggccaa acaggatatc 
      361 tgtggtaagc agttcctgcc ccggctcggg gccaagaaca gatggtcccc agatgcggtc 
      421 cagccctcag cagtttctag tgaatcatca gatgtttcca gggtgcccca aggacctgaa 
      481 aatgaccctg taccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg 
      541 cttccgctct ccgagctcaa taaaagagcc cacaacccct cactcggcgc gccagtcttc 
      601 cgatagactg cgtcgcccgg gtacccgtat tcccaataaa gcctcttgct gtttgcatcc 
      661 gaatcgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccacgacg 
      721 ggggtctttc atttgggggc tcgtccggga tttggagacc cctgcccagg gaccaccgac 
      781 ccaccaccgg gaggtaagct ggccagcaac ttatctgtgt ctgtccgatt gtctagtgtc 
      841 tatgtttgat gttatgcgcc tgcgtctgta ctagttagct aactagctct gtatctggcg 
      901 gacccgtggt ggaactgacg agttctgaac acccggccgc aaccctggga gacgtcccag 
      961 ggactttggg ggccgttttt gtggcccgac ctgaggaagg gagtcgatgt ggaatccgac 
     1021 cccgtcagga tatgtggttc tggtaggaga cgagaaccta aaacagttcc cgcctccgtc 
     1081 tgaatttttg ctttcggttt ggaaccgaag ccgcgcgtct tgtctgctgc agcgctgcag 
     1141 catcgttctg tgttgtctct gtctgactgt gtttctgtat ttgtctgaaa attagggcca 
     1201 gactgttacc actcccttaa gtttgacctt aggtcactgg aaagatgtcg agcggatcgc 
     1261 tcacaaccag tcggtagatg tcaagaagag acgttgggtt accttctgct ctgcagaatg 
     1321 gccaaccttt aacgtcggat ggccgcgaga cggcaccttt aaccgagacc tcatcaccca 
     1381 ggttaagatc aaggtctttt cacctggccc gcatggacac ccagaccagg tcccctacat 
     1441 cgtgacctgg gaagccttgg cttttgaccc ccctccctgg gtcaagccct ttgtacaccc 
     1501 taagcctccg cctcctcttc ctccatccgc cccgtctctc ccccttgaac ctcctcgttc 
     1561 gaccccgcct cgatcctccc tttatccagc cctcactcct tctctaggcg ccggaattcc 
     1621 gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg 
     1681 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa 
     1741 tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg 
     1801 tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt 
     1861 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa 
     1921 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc 
     1981 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg 
     2041 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg 
     2101 aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg 
     2161 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg 
     2221 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact 
     2281 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg 
     2341 ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc 
     2401 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggactct 
     2461 ggggttcgaa atgaccgacc aagcgacgcc caacctgcca tcacgagatt tcgattccac 
     2521 cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat 
     2581 cctccagcgc ggggatctca tgctggagtt cttcgcccac cccgggctcg atcccctcgc 
     2641 gagttggttc agctgctgcc tgaggctgga cgacctcgcg gagttctacc ggcagtgcaa 
     2701 atccgtcggc atccaggaaa ccagcagcgg ctatccgcgc atccatgccc ccgaactgca 
     2761 ggagtgggga ggcacgatgg ccgctttggt cgaggcggat cctagccccc acgaaccata 
     2821 aaccattccc catgggggac cccgtcccta acccacgggg ccagtggcta tggcagggcc 
     2881 tgccgccccg acgttggctg cgagccctgg gccttcaccc gaacttgggg ggtggggtgg 
     2941 ggaaaaggaa gaaacgcggg cgtattggcc ccaatggggt ctcggtgggg tatcgacaga 
     3001 gtgccagccc tgggaccgaa ccccgcgttt atgaacaaac gacccaacac cgtgcgtttt 
     3061 attctgtctt tttattgccg tcatagcgcg ggttccttcc ggtattgtct ccttccgtgt 
     3121 ttcagttctt aagaattgcg gccgcgatcg cgttaacaga tctggccgca agcttggttg 
     3181 gaatctaaaa tacacaaaca attaggatcc ctgaggagca gttctttgat ttgcaccacc 
     3241 aggagcctca aatttttcaa taaattcacc tgactgcaca ttaggacttt ggctttgagc 
     3301 ttcccacctc tcagttactt gctgaatact gtctgcttca tcaatattat cataggtgtg 
     3361 cccaaatgat atttgcaacc cttccctgtt ggctacttgt ctcaccattg taggcctaat 
     3421 gggagacgga tccgtagttt aacacattat acactagggt cgactgtgga attgtgagcg 
     3481 ctcacaattc cacaacctag ggtcgactgt ggaattgtga gcgctcacaa ttccacaacc 
     3541 tagggtcgac tgtggaattg tgagcgctca caattccaca acctagtaaa ttttatattt 
     3601 accttagagc tttaaatctc tgtaggtagt ttgtccaagc ttagatcaac aagcttgggg 
     3661 ccgcggaggc tggatcggtc ccggtgtctt ctatggaggt caaaacagcg tggatggcgt 
     3721 ctccaggcga tctgacggtt cactaaacga gctctgctta tatagacctc ccaccgtaca 
     3781 tgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt 
     3841 tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc 
     3901 cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta 
     3961 atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac 
     4021 tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc 
     4081 atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga 
     4141 cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat 
     4201 gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg 
     4261 aactccatat atgggctatg aactaatgac cccgtaattg attactatta ataactagtc 
     4321 aataatcaat gtcaacatgg cggtaatgtt ggacatgagc caatataaat gtacatatta 
     4381 tgatatggat acaacgtatg caatggccaa tagccaatat tgatttatgc tatataacca 
     4441 atgaataata tggctaatgg ccggatcgtg gagctcgcgc gaacatcgat aaaataaaag 
     4501 attttattta gtctccagaa aaagggggga atgaaagacc ccacctgtag gtttggcaag 
     4561 ctagcttaag taacgccatt ttgcaaggca tggaaaaata cataactgag aatagagaag 
     4621 ttcagatcaa ggtcaggaac agatggaaca gctgaatatg ggccaaacag gatatctgtg 
     4681 gtaagcagtt cctgccccgg ctcagggcca agaacagatg gaacagctga atatgggcca 
     4741 aacaggatat ctgtggtaag cagttcctgc cccggctcag ggccaagaac agatggtccc 
     4801 cagatgcggt ccagccctca gcagtttcta gagaaccatc agatgtttcc agggtgcccc 
     4861 aaggacctga aatgaccctg tgccttattt gaactaacca atcagttcgc ttctcgcttc 
     4921 tgttcgcgcg cttctgctcc ccgagctcaa taaaagagcc cacaacccct cactcggggc 
     4981 gccagtcctc cgattgactg agtcgcccgg gtacccgtgt atccaataaa ccctcttgca 
     5041 gttgcatccg acttgtggtc tcgctgttcc ttgggagggt ctcctctgag tgattgacta 
     5101 cccgtcagcg ggggtctttc atttgggggc tcgtccggga tcgggagacc cctgcccagg 
     5161 gaccaccgac ccaccaccgg gaggtaagct ggctgcctcg cgcgtttcgg tgatgacggt 
     5221 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc 
     5281 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc 
     5341 atgacccagt cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc 
     5401 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa 
     5461 aataccgcat caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc 
     5521 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag 
     5581 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa 
     5641 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc 
     5701 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc 
     5761 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg 
     5821 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt 
     5881 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc 
     5941 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc 
     6001 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag 
     6061 agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg 
     6121 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa 
     6181 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag 
     6241 gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact 
     6301 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa 
     6361 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt 
     6421 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag 
     6481 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca 
     6541 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc 
     6601 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt 
     6661 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg 
     6721 ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca 
     6781 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg 
     6841 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca 
     6901 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg 
     6961 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct 
     7021 cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca 
     7081 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca 
     7141 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg 
     7201 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac 
     7261 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt 
     7321 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc 
     7381 cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat 
     7441 taacctataa aaataggcgt atcacgaggc cctttcgtct tcaa 
//
