LOCUS       pEF1mBID                6750 bp    DNA     circular     11-MAR-2008
DEFINITION  LMBP 4325.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4325
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-MAR-2008
            
COMMENT     VNTDATE|476108273|
COMMENT     VNTNAME|pEF1mBID|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF1mBID" 0 6750 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "EcoRI" "XhoI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 1596 1595 1 6750 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #3=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1749 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 2 1439 0 2362 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 4 773 0 2298 0 3177 0 
COMMENT            6549 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "intron hEF1 promoter" 15 0 0 704 1642 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "704..1642") 
COMMENT        #7=(CFSignal (CObList) "T7 promoter" 30 0 0 1668 1684 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1668..1684") 
COMMENT        #8=(CFSignal (CObList) "hEF1 promoter" 29 0 0 508 1651 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "508..1651") 
COMMENT        #9=(CFSignal (CObList) "lac promoter" 30 0 1 4769 4852 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "ampicillin" 4 0 1 5876 6736 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5296..6156)") 
COMMENT        #11=(CFSignal (CObList) "pMB1 ori" 33 0 1 4944 5335 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4364..4755)") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4556 4630 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(3976..4050)") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4556 4630 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3976..4050") 
COMMENT        #14=(CFSignal (CObList) "SV40 ori" 33 0 0 3419 3494 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2839..2914") 
COMMENT        #15=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3181 3462 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2601..2882") 
COMMENT        #16=(CFSignal (CObList) "f1 ori" 33 0 0 2748 3176 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2168..2596") 
COMMENT        #17=(CFSignal (CObList) "BGH polyA" 43 0 0 2478 2702 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1898..2122") 
COMMENT        #18=(CFSignal (CObList) "His-tag" 22 0 0 2432 2449 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1852..1869") 
COMMENT        #19=(CFSignal (CObList) "V5 epitope" 22 0 0 2381 2422 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1801..1842") 
COMMENT        #20=(CFSignal (CObList) "mBID" 4 0 0 1754 2341 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #21=(CFSignal (CObList) "neomycin" 4 0 0 3586 4380 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3586..4380")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #22=(CFSignal (CObList) "LMBP 4325" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #23=(CFSignal (CObList) "Last modified: 11-MAR-2008" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                          "DNA Plasmid 'pEF1mBID'" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4325." 
COMMENT                          1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Local object" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 03/06/08 12:17" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 03/11/08 11:50" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6750 bp" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 6-MAR-2008" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 4325") 
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COMMENT                                 "" 1))) "Last modified: 11-MAR-2008"))) 
COMMENT                   "Annotations") 
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COMMENT                     #45=(CFolderPar 
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COMMENT                                        "Start: 1754  End: 2341" 1))) "mBID") 
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COMMENT                                        "Start: 3586  End: 4380" 1) 
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COMMENT                                        "Tn5 antibiotic resistance gene; coding sequence" 
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COMMENT                                        "Start: 5876  End: 6736 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #55=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
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COMMENT                                   #61=(CLinePar 
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COMMENT                                   #75=(CLinePar 
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COMMENT                                        "   508..1651" 1))) "hEF1 promoter") 
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COMMENT                                   #78=(CLinePar 
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COMMENT                                   #79=(CLinePar 
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COMMENT                                        "   2601..2882" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                   #83=(CLinePar 
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COMMENT                                   #84=(CLinePar 
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COMMENT                                        "   1668..1684" 1))) "T7 promoter") 
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COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                                        "   2168..2596" 1))) "f1 ori") 
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COMMENT                                        "   complement(4364..4755)" 1))) 
COMMENT                                 "pMB1 ori"))) "Replication Origin (3 total)") 
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COMMENT                                           "   complement(3976..4050)" 1))) 
COMMENT                                   "SV40 polyA late") 
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COMMENT                                           "   3976..4050" 1))) 
COMMENT                                   "SV40 polyA early"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                                            " N1: 1749 " 1))))) "EcoRI: 1 site") 
COMMENT                            1 5 "GAATTC" "CTTAAG") 
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FEATURES             Location/Qualifiers
     intron          704..1642
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     promoter        1668..1684
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        508..1651
                     /vntifkey="29"
                     /label=hEF1\promoter
     promoter        complement(4769..4852)
                     /vntifkey="30"
                     /label=lac\promoter
     CDS             complement(5876..6736)
                     /vntifkey="4"
                     /label=ampicillin
     rep_origin      complement(4944..5335)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             3586..4380
                     /vntifkey="4"
                     /label=neomycin
                     /note="Tn5 antibiotic resistance gene; coding sequence"
     terminator      complement(4556..4630)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     terminator      4556..4630
                     /vntifkey="43"
                     /label=SV40\polyA\early
     rep_origin      3419..3494
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        3181..3462
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      2748..3176
                     /vntifkey="33"
                     /label=f1\ori
     terminator      2478..2702
                     /vntifkey="43"
                     /label=BGH\polyA
     misc_marker     2432..2449
                     /vntifkey="22"
                     /label=His-tag
     misc_marker     2381..2422
                     /vntifkey="22"
                     /label=V5\epitope
     CDS             1754..2341
                     /vntifkey="4"
                     /label=mBID
BASE COUNT     1492 a      1763 c      1832 g      1663 t 
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 
     1741 tgtggtggaa ttcatggact ctgaggtcag caacggttcc ggcctggggg ccaagcacat 
     1801 cacagacctg ctggtgttcg gctttctcca aagctctggc tgtactcgcc aagagctgga 
     1861 ggtgctgggt cgggaactgc ctgtgcaagc ttactgggag gcagacctcg aagacgagct 
     1921 gcagacagac ggcagccagg ccagccgctc cttcaaccaa ggaagaatag agccagattc 
     1981 tgaaagtcag gaagaaatca tccacaacat tgccagacat ctcgcccaaa taggcgatga 
     2041 gatggaccac aacatccagc ccacactggt gagacagcta gccgcacagt tcatgaatgg 
     2101 cagcctgtcg gaggaagaca aaaggaactg cctggccaaa gcccttgatg aggtgaagac 
     2161 agccttcccc agagacatgg agaacgacaa ggccatgctg ataatgacaa tgctgttggc 
     2221 caaaaaagtg gccagtcacg caccatcttt gctccgtgat gtcttccaca cgactgtcaa 
     2281 ctttattaac cagaacctat tctcctatgt gaggaacttg gttagaaacg agatggactg 
     2341 atccagcaca gtggcggccg ctcgaggtca cccattcgaa ggtaagccta tccctaaccc 
     2401 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac 
     2461 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 
     2521 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 
     2581 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 
     2641 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 
     2701 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 
     2761 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 
     2821 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 
     2881 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 
     2941 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 
     3001 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 
     3061 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 
     3121 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 
     3181 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 
     3241 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 
     3301 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 
     3361 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
     3421 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 
     3481 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 
     3541 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 
     3601 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 
     3661 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 
     3721 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 
     3781 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 
     3841 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 
     3901 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 
     3961 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 
     4021 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 
     4081 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 
     4141 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 
     4201 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 
     4261 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 
     4321 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 
     4381 gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 
     4441 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 
     4501 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 
     4561 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 
     4621 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 
     4681 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 
     4741 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 
     4801 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 
     4861 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 
     4921 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 
     4981 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 
     5041 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccaggaac cgtaaaaagg 
     5101 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     5161 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     5221 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     5281 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     5341 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     5401 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     5461 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     5521 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 
     5581 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     5641 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     5701 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     5761 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     5821 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     5881 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     5941 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     6001 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     6061 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     6121 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     6181 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     6241 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     6301 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     6361 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     6421 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     6481 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     6541 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     6601 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     6661 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     6721 aatgttgaat actcatactc ttcctttttc 
//
