ID pEF1mBID standard; circular DNA; ; 6750 BP. DE LMBP 4325. CC Accession number: LMBP 4325 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-MAR-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT intron 704..1642 FT /vntifkey="15" FT /label=intron\hEF1\promoter FT promoter 1668..1684 FT /vntifkey="30" FT /label=T7\promoter FT promoter 508..1651 FT /vntifkey="29" FT /label=hEF1\promoter FT promoter complement(4769..4852) FT /vntifkey="30" FT /label=lac\promoter FT CDS complement(5876..6736) FT /vntifkey="4" FT /label=ampicillin FT rep_origin complement(4944..5335) FT /vntifkey="33" FT /label=pMB1\ori FT CDS 3586..4380 FT /vntifkey="4" FT /label=neomycin FT /note="Tn5 antibiotic resistance gene; coding sequence" FT terminator complement(4556..4630) FT /vntifkey="43" FT /label=SV40\polyA\late FT terminator 4556..4630 FT /vntifkey="43" FT /label=SV40\polyA\early FT rep_origin 3419..3494 FT /vntifkey="33" FT /label=SV40\ori FT promoter 3181..3462 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 2748..3176 FT /vntifkey="33" FT /label=f1\ori FT terminator 2478..2702 FT /vntifkey="43" FT /label=BGH\polyA FT misc_marker 2432..2449 FT /vntifkey="22" FT /label=His-tag FT misc_marker 2381..2422 FT /vntifkey="22" FT /label=V5\epitope FT CDS 1754..2341 FT /vntifkey="4" FT /label=mBID SQ Sequence 6750 BP; 1492 A; 1763 C; 1832 G; 1663 t; aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 60 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 120 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 180 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 240 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 300 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 360 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 420 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 480 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 540 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 600 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 660 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 720 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 780 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 840 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 900 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 960 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 1020 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 1080 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 1140 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 1200 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1260 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1320 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1380 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1440 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1500 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1560 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1620 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 1680 tatagggaga cccaagctgg ctaggtaagc ttggtaccga gctcggatcc actagtccag 1740 tgtggtggaa ttcatggact ctgaggtcag caacggttcc ggcctggggg ccaagcacat 1800 cacagacctg ctggtgttcg gctttctcca aagctctggc tgtactcgcc aagagctgga 1860 ggtgctgggt cgggaactgc ctgtgcaagc ttactgggag gcagacctcg aagacgagct 1920 gcagacagac ggcagccagg ccagccgctc cttcaaccaa ggaagaatag agccagattc 1980 tgaaagtcag gaagaaatca tccacaacat tgccagacat ctcgcccaaa taggcgatga 2040 gatggaccac aacatccagc ccacactggt gagacagcta gccgcacagt tcatgaatgg 2100 cagcctgtcg gaggaagaca aaaggaactg cctggccaaa gcccttgatg aggtgaagac 2160 agccttcccc agagacatgg agaacgacaa ggccatgctg ataatgacaa tgctgttggc 2220 caaaaaagtg gccagtcacg caccatcttt gctccgtgat gtcttccaca cgactgtcaa 2280 ctttattaac cagaacctat tctcctatgt gaggaacttg gttagaaacg agatggactg 2340 atccagcaca gtggcggccg ctcgaggtca cccattcgaa ggtaagccta tccctaaccc 2400 tctcctcggt ctcgattcta cgcgtaccgg tcatcatcac catcaccatt gagtttaaac 2460 ccgctgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt gcccctcccc 2520 cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat aaaatgagga 2580 aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg tggggcagga 2640 cagcaagggg gaggattggg aagacaatag caggcatgct ggggatgcgg tgggctctat 2700 ggcttctgag gcggaaagaa ccagctgggg ctctaggggg tatccccacg cgccctgtag 2760 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag 2820 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt 2880 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca 2940 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata 3000 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca 3060 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc 3120 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattaatt 3180 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt 3240 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 3300 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 3360 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 3420 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 3480 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 3540 tccattttcg gatctgatca agagacagga tgaggatcgt ttcgcatgat tgaacaagat 3600 ggattgcacg caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca 3660 caacagacaa tcggctgctc tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg 3720 gttctttttg tcaagaccga cctgtccggt gccctgaatg aactgcagga cgaggcagcg 3780 cggctatcgt ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact 3840 gaagcgggaa gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct 3900 caccttgctc ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg 3960 cttgatccgg ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt 4020 actcggatgg aagccggtct tgtcgatcag gatgatctgg acgaagagca tcaggggctc 4080 gcgccagccg aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc 4140 gtgacccatg gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga 4200 ttcatcgact gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc 4260 cgtgatattg ctgaagagct tggcggcgaa tgggctgacc gcttcctcgt gctttacggt 4320 atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga 4380 gcgggactct ggggttcgcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga 4440 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 4500 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 4560 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 4620 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 4680 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 4740 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 4800 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 4860 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 4920 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 4980 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5040 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccaggaac cgtaaaaagg 5100 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 5160 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 5220 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 5280 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 5340 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 5400 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 5460 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 5520 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 5580 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 5640 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 5700 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 5760 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 5820 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 5880 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 5940 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 6000 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 6060 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 6120 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 6180 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 6240 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 6300 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6360 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6420 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 6480 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 6540 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 6600 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 6660 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 6720 aatgttgaat actcatactc ttcctttttc 6750 //