LOCUS       pRS425GAL1mBAX 8323 bp dsDNA   Circular
DEFINITION  LMBP 4326.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     rep_origin      complement(2474..2930)
                     /label=f1\ori
     promoter        3100..3119
                     /label=T7\promoter
     CDS             complement(666..1757)
                     /note="S. cerevisiae LEU2 coding sequence"
                     /label=LEU2
     promoter        3312..3756
                     /note="Saccharomyces cerevisiae galactokinase promoter (GAL1)"
                     /label=GAL1\promoter
     terminator      4585..4688
                     /note="Saccharomyces cerevisiae 2 micron plasmid (2µ) FLP terminator"
                     /label=FLP\terminator
     CDS             3803..4378
                     /note="Mouse Bax cDNA encoding the Bax-alpha protein (mBAX)"
                     /label=mBAX
     promoter        complement(4720..4739)
                     /label=T3\promoter
     promoter        complement(4765..4886)
                     /label=lac\promoter
     rep_origin      4980..5920
                     /note="origin of replication"
                     /label=pMB1\ori
     rep_origin      6913..8258
                     /label=2mu\ori
     CDS             complement(5924..6781)
                     /note="antibiotic resistance gene"
                     /label=AMP
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc 
      241 accattatgg gaaatgcttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca 
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat 
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc 
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc 
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt 
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg 
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc 
     1141 acagtttttc tccataatct tgaagaggcc aaaagattag ctttatccaa ggaccaaata 
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     1501 ctaccggtac cccatttagg accagccaca gcacctaaca aaacggcatc aaccttcttg 
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata 
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat 
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat 
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct 
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca 
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat 
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga 
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg 
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt 
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg 
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta 
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa 
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt 
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa 
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag 
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt 
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac 
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag 
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg 
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg 
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc 
     3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc 
     3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc 
     3121 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc 
     3181 ctgcagcccg ggggatccac tagttctaga gcggccgcca ccgcggtgga gagcggaaga 
     3241 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagagatcc 
     3301 ggacggatta gaagccgccg agcgggtgac agccctccga aggaagactc tcctccgtgc 
     3361 gtcctcgtct tcaccggtcg cgttcctgaa acgcagatgt gcctcgcgcc gcactgctcc 
     3421 gaacaataaa gattctacaa tactagcttt tatggttatg aagaggaaaa attggcagta 
     3481 acctggcccc acaaaccttc aaatgaacga atcaaattaa caaccatagg atgataatgc 
     3541 gattagtttt ttagccttat ttctggggta attaatcagc gaagcgatga tttttgatct 
     3601 attaacagat atataaatgc aaaaactgca taaccacttt aactaatact ttcaacattt 
     3661 tcggtttgta ttacttctta ttcaaatgta ataaaagtat caacaaaaaa ttgttaatat 
     3721 acctctatac tttaacgtca aggagaaaaa acgatcgtga ctaaatctca ttcagaagaa 
     3781 gtgattgtga tcctctagag tcatggacgg gtccggggag cagcttggga gcggcgggcc 
     3841 caccagctct gaacagatca tgaagacagg ggcctttttg ctacagggtt tcatccagga 
     3901 tcgagcaggg aggatggctg gggagacacc tgagctgacc ttggagcagc cgccccagga 
     3961 tgcgtccacc aagaagctga gcgagtgtct ccggcgaatt ggagatgaac tggatagcaa 
     4021 tatggagctg cagaggatga ttgctgacgt ggacacggac tccccccgag aggtcttctt 
     4081 ccgggtggca gctgacatgt ttgctgatgg caacttcaac tggggccgcg tggttgccct 
     4141 cttctacttt gctagcaaac tggtgctcaa ggccctgtgc actaaagtgc ccgagctgat 
     4201 cagaaccatc atgggctgga cactggactt cctccgtgag cggctgcttg tctggatcca 
     4261 agaccagggt ggctgggaag gcctcctctc ctacttcggg acccccacat ggcagacagt 
     4321 gaccatcttt gtggctggag tcctcaccgc ctcgctcacc atctggaaga agatgggctg 
     4381 agacctgcag gcatgcaagc ttatgatcca atatcaaagg aaatgatagc attgaaggat 
     4441 gagactaatc caattgagga gtggcagcat atagaacagc taaagggtag tgctgaagga 
     4501 agcatacgat accccgcatg gaatgggata atatcacagg aggtactaga ctacctttca 
     4561 tcctacataa atagacgcat ataagtacgc atttaagcat aaacacgcac tatgccgttc 
     4621 ttctcatgta tatatatata caggcaacac gcagatatag gtgcgacgtg aacagtgagc 
     4681 tgtatgtgca gcttgcaaat taaagctcca gcttttgttc cctttagtga gggttaattg 
     4741 cgcgcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa 
     4801 ttccacacaa cataggagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga 
     4861 ggtaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt 
     4921 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct 
     4981 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat 
     5041 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga 
     5101 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt 
     5161 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt 
     5221 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc 
     5281 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa 
     5341 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct 
     5401 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta 
     5461 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg 
     5521 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc 
     5581 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta 
     5641 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg 
     5701 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt 
     5761 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg 
     5821 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta 
     5881 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg 
     5941 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg 
     6001 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc 
     6061 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg 
     6121 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg 
     6181 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag 
     6241 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat 
     6301 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc 
     6361 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc 
     6421 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa 
     6481 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac 
     6541 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt 
     6601 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc 
     6661 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa 
     6721 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca 
     6781 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat 
     6841 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa 
     6901 aagtgccacc tgaacgaagc atctgtgctt cattttgtag aacaaaaatg caacgcgaga 
     6961 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg 
     7021 aaagcgctat tttaccaacg aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg 
     7081 cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca 
     7141 acgcgagagc gctattttac caacaaagaa tctatacttc ttttttgttc tacaaaaatg 
     7201 catcccgaga gcgctatttt tctaacaaag catcttagat tacttttttt ctcctttgtg 
     7261 cgctctataa tgcagtctct tgataacttt ttgcactgta ggtccgttaa ggttagaaga 
     7321 aggctacttt ggtgtctatt ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt 
     7381 tactgattac tagcgaagct gcgggtgcat tttttcaaga taaaggcatc cccgattata 
     7441 ttctataccg atgtggattg cgcatacttt gtgaacagaa agtgatagcg ttgatgattc 
     7501 ttcattggtc agaaaattat gaacggtttc ttctattttg tctctatata ctacgtatag 
     7561 gaaatgttta cattttcgta ttgttttcga ttcactctat gaatagttct tactacaatt 
     7621 tttttgtcta aagagtaata ctagagataa acataaaaaa tgtagaggtc gagtttagat 
     7681 gcaagttcaa ggagcgaaag gtggatgggt aggttatata gggatatagc acagagatat 
     7741 atagcaaaga gatacttttg agcaatgttt gtggaagcgg tattcgcaat attttagtag 
     7801 ctcgttacag tccggtgcgt ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt 
     7861 tttcaaaagc gctctgaagt tcctatactt tctagagaat aggaacttcg gaataggaac 
     7921 ttcaaagcgt ttccgaaaac gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag 
     7981 ctcactgttc acgtcgcacc tatatctgcg tgttgcctgt atatatatat acatgagaag 
     8041 aacggcatag tgcgtgttta tgcttaaatg cgtacttata tgcgtctatt tatgtaggat 
     8101 gaaaggtagt ctagtacctc ctgtgatatt atcccattcc atgcggggta tcgtatgctt 
     8161 ccttcagcac taccctttag ctgttctata tgctgccact cctcaattgg attagtctca 
     8221 tccttcaatg ctatcatttc ctttgatatt ggatcatact aagaaaccat tattatcatg 
     8281 acattaacct ataaaaatag gcgtatcacg aggccctttc gtc 
//
