ID pREP4FLAG standard; circular DNA; ; 10189 BP. DE LMBP 4343. CC Accession number: LMBP 4343 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-APR-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 418..2343 FT /vntifkey="4" FT /label=EBNA1 FT /note="Epstein-Barr virus nuclear antigen 1" FT rep_origin 2649..4420 FT /vntifkey="33" FT /label=Epstein-Barr\oriP FT /note="Epstein-Barr virus origin of replication (oriP)" FT terminator complement(5166..5262) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator 5166..5262 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT misc_marker complement(5330..5353) FT /vntifkey="22" FT /label=FLAG\epitope FT /note="FLAG epitope tag" FT promoter complement(5389..5571) FT /vntifkey="29" FT /label=RSV-LTR FT /note="Rous sarcoma virus long terminal repeat" FT terminator complement(6442..6511) FT /vntifkey="43" FT /label=HSV-TK\polyA FT /note="Herpes simplex virus thymidine kinase polyadenylation signal" FT CDS complement(6535..7560) FT /vntifkey="4" FT /label=hygromycin FT /note="antibiotic resistance gene; coding sequence" FT promoter complement(7631..7741) FT /vntifkey="29" FT /label=HSV-TK\promoter FT /note="Herpes simplex virus thymidine kinase promoter" FT rep_origin complement(8318..8580) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(9121..9981) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 10189 BP; 2496 A; 2545 C; 2906 G; 2242 t; ttctcatgtt tgacagctta tcatcgataa gctgatcctc acaggccgca cccagctttt 60 cttccgttgc cccagtagca tctctgtctg gtgaccttga agaggaagag gaggggtccc 120 gagaatcccc atccctaccg tccagcaaaa agggggacga ggaatttgag gcctggcttg 180 aggctcagga cgcaaatctt gaggatgttc agcgggagtt ttccgggctg cgagtaattg 240 gtgatgagga cgaggatggt tcggaggatg gggaattttc agacctggat ctgtctgaca 300 gcgaccatga aggggatgag ggtggggggg ctgttggagg gggcaggagt ctgcactccc 360 tgtattcact gagcgtcgtc taataaagat gtctattgat ctcttttagt gtgaatcatg 420 tctgacgagg ggccaggtac aggacctgga aatggcctag gagagaaggg agacacatct 480 ggaccagaag gctccggcgg cagtggacct caaagaagag ggggtgataa ccatggacga 540 ggacggggaa gaggacgagg acgaggaggc ggaagaccag gagccccggg cggctcagga 600 tcagggccaa gacatagaga tggtgtccgg agaccccaaa aacgtccaag ttgcattggc 660 tgcaaaggga cccacggtgg aacaggagca ggagcaggag cgggaggggc aggagcagga 720 ggggcaggag caggaggagg ggcaggagca ggaggagggg caggaggggc aggaggggca 780 ggaggggcag gagcaggagg aggggcagga gcaggaggag gggcaggagg ggcaggaggg 840 gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg agcaggagga 900 ggggcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg aggaggggca 960 ggaggggcag gagcaggagg aggggcagga ggggcaggag gggcaggagc aggaggaggg 1020 gcaggagcag gaggggcagg aggggcagga ggggcaggag caggaggggc aggagcagga 1080 ggaggggcag gaggggcagg aggggcagga gcaggagggg caggagcagg aggggcagga 1140 gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggggc aggaggggca 1200 ggagcaggag gggcaggagg ggcaggagca ggaggagggg caggaggggc aggagcagga 1260 ggaggggcag gaggggcagg agcaggaggg gcaggagggg caggagcagg aggggcagga 1320 ggggcaggag caggaggggc aggaggggca ggagcaggag gaggggcagg agcaggaggg 1380 gcaggagcag gaggtggagg ccggggtcga ggaggcagtg gaggccgggg tcgaggaggt 1440 agtggaggcc ggggtcgagg aggtagtgga ggccgccggg gtagaggacg tgaaagagcc 1500 agggggggaa gtcgtgaaag agccaggggg agaggtcgtg gacgtggaga aaagaggccc 1560 aggagtccca gtagtcagtc atcatcatcc gggtctccac cgcgcaggcc ccctccaggt 1620 agaaggccat ttttccaccc tgtaggggaa gccgattatt ttgaatacca ccaagaaggt 1680 ggcccagatg gtgagcctga cgtgcccccg ggagcgatag agcagggccc cgcagatgac 1740 ccaggagaag gcccaagcac tggaccccgg ggtcagggtg atggaggcag gcgcaaaaaa 1800 ggagggtggt ttggaaagca tcgtggtcaa ggaggttcca acccgaaatt tgagaacatt 1860 gcagaaggtt taagagctct cctggctagg agtcacgtag aaaggactac cgacgaagga 1920 acttgggtcg ccggtgtgtt cgtatatgga ggtagtaaga cctcccttta caacctaagg 1980 cgaggaactg cccttgctat tccacaatgt cgtcttacac cattgagtcg tctccccttt 2040 ggaatggccc ctggacccgg cccacaacct ggcccgctaa gggagtccat tgtctgttat 2100 ttcatggtct ttttacaaac tcatatattt gctgaggttt tgaaggatgc gattaaggac 2160 cttgttatga caaagcccgc tcctacctgc aatatcaggg tgactgtgtg cagctttgac 2220 gatggagtag atttgcctcc ctggtttcca cctatggtgg aaggggctgc cgcggagggt 2280 gatgacggag atgacggaga tgaaggaggt gatggagatg agggtgagga agggcaggag 2340 tgatgtaact tgttaggaga cgccctcaat cgtattaaaa gccgtgtatt cccccgcact 2400 aaagaataaa tccccagtag acatcatgcg tgctgttggt gtatttctgg ccatctgtct 2460 tgtcaccatt ttcgtcctcc caacatgggg caattgggca tacccatgtt gtcacgtcac 2520 tcagctccgc gctcaacacc ttctcgcgtt ggaaaacatt agcgacattt acctggtgag 2580 caatcagaca tgcgacggct ttagcctggc ctccttaaat tcacctaaga atgggagcaa 2640 ccagcatgca ggaaaaggac aagcagcgaa aattcacgcc cccttgggag gtggcggcat 2700 atgcaaagga tagcactccc actctactac tgggtatcat atgctgactg tatatgcatg 2760 aggatagcat atgctacccg gatacagatt aggatagcat atactaccca gatatagatt 2820 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatataaatt 2880 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt 2940 aggatagcct atgctaccca gatatagatt aggatagcat atgctaccca gatatagatt 3000 aggatagcat atgctatcca gatatttggg tagtatatgc tacccagata taaattagga 3060 tagcatatac taccctaatc tctattagga tagcatatgc tacccggata cagattagga 3120 tagcatatac tacccagata tagattagga tagcatatgc tacccagata tagattagga 3180 tagcctatgc tacccagata taaattagga tagcatatac tacccagata tagattagga 3240 tagcatatgc tacccagata tagattagga tagcctatgc tacccagata tagattagga 3300 tagcatatgc tatccagata tttgggtagt atatgctacc catggcaaca ttagcccacc 3360 gtgctctcag cgacctcgtg aatatgagga ccaacaaccc tgtgcttggc gctcaggcgc 3420 aagtgtgtgt aatttgtcct ccagatcgca gcaatcgcgc ccctatcttg gcccgcccac 3480 ctacttatgc aggtattccc cggggtgcca ttagtggttt tgtgggcaag tggtttgacc 3540 gcagtggtta gcggggttac aatcagccaa gttattacac ccttatttta cagtccaaaa 3600 ccgcagggcg gcgtgtgggg gctgacgcgt gcccccactc cacaatttca aaaaaaagag 3660 tggccacttg tctttgttta tgggccccat tggcgtggag ccccgtttaa ttttcggggg 3720 tgttagagac aaccagtgga gtccgctgct gtcggcgtcc actctctttc cccttgttac 3780 aaatagagtg taacaacatg gttcacctgt cttggtccct gcctgggaca catcttaata 3840 accccagtat catattgcac taggattatg tgttgcccat agccataaat tcgtgtgaga 3900 tggacatcca gtctttacgg cttgtcccca ccccatggat ttctattgtt aaagatattc 3960 agaatgtttc attcctacac tagtatttat tgcccaaggg gtttgtgagg gttatattgg 4020 tgtcatagca caatgccacc actgaacccc ccgtccaaat tttattctgg gggcgtcacc 4080 tgaaaccttg ttttcgagca cctcacatac accttactgt tcacaactca gcagttattc 4140 tattagctaa acgaaggaga atgaagaagc aggcgaagat tcaggagagt tcactgcccg 4200 ctccttgatc ttcagccact gcccttgtga ctaaaatggt tcactaccct cgtggaatcc 4260 tgaccccatg taaataaaac cgtgacagct catggggtgg gagatatcgc tgttccttag 4320 gaccctttta ctaaccctaa ttcgatagca tatgcttccc gttgggtaac atatgctatt 4380 gaattagggt tagtctggat agtatatact actacccggg aagcatatgc tacccgttta 4440 gggttaacaa gggggcctta taaacactat tgctaatgcc ctcttgaggg tccgcttatc 4500 ggtagctaca caggcccctc tgattgacgt tggtgtagcc tcccgtagtc ttcctgggcc 4560 cctgggaggt acatgtcccc cagcattggt gtaagagctt cagccaagag ttacacataa 4620 aggcaatgtt gtgttgcagt ccacagactg caaagtctgc tccaggatga aagccactca 4680 gtgttggcaa atgtgcacat ccatttataa ggatgtcaac tacagtcaga gaaccccttt 4740 gtgtttggtc cccccccgtg tcacatgtgg aacagggccc agttggcaag ttgtaccaac 4800 caactgaagg gattacatgc actgccccga atacaaaaca aaagcgctcc tcgtaccagc 4860 gaagaagggg cagagatgcc gtagtcaggt ttagttcgtc cggcggcggg gctctagagt 4920 cgaccggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct 4980 tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt 5040 cagaggtttt caccgtcatc accgaaacgc gcgaggcagc cggatcataa tcagccatac 5100 cacatttgta gaggttttac ttgctttaaa aaacctcccc acctccccct gaacctgaaa 5160 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 5220 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 5280 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcggatcctc ttgtcatcgt 5340 cgtccttgta gtccatggta gcttgctagc agctggtacc cagcttggag gtgcacacca 5400 atgtggtgaa tggtcaaatg gcgtttattg tatcgagcta ggcacttaaa tacaattatc 5460 tctgcaatgc ggaattcagt ggttcgtcca atccatgtca gacctgtctg ttgccttcct 5520 aataaggcac gatcgtacca ccttacttcc accaatcggc atgcacggtg ctttttctct 5580 ccttgtaagg catgttgcta actcatcgtt accatgttgc aagactacaa gtgtattgca 5640 taagactaca tttccccctc cctatgcaaa agcgaaacta ctatatcctg aggggactcc 5700 taaccgcgta caaccgaagc cccgctgggc gcctaaacac accctagtcc cctcagatac 5760 gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg cctaacccta agcagattct 5820 tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta aattttgctc gcgcactact 5880 cagcgacctc caacacacaa gcagggagca gatactggct taactatgcg gcagcagagc 5940 agattgtact gagagtgcac catacaagct caggatctgc gatgataagc tgtcaaacat 6000 gagaattggt cgaccactgg gcgccagaaa tccgcgcggt ggtttttggg ggtcgggggt 6060 gtttggcagc cacagacgcc cggtgttcgt gtcgcgccag tacatgcggt ccatgcccag 6120 gccatccaaa aaccatgggt ctgtctgctc agtccagtcg tggaccagac cccacgcaac 6180 gcccaaaata ataaccccca cgaaccataa accattcccc atgggggacc ccgtccctaa 6240 cccacggggc cagtggctat ggcagggcct gccgccccga cgttggctgc gagccctggg 6300 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc 6360 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta 6420 tgaacaaacg acccaacacc cgtgcgtttt attctgtctt tttattgccg tcatagcgcg 6480 ggttccttcc ggtattgtct ccttccgtgt ttcagttagc ctcccccatc tcccctattc 6540 ctttgccctc ggacgagtgc tggggcgtcg gtttccacta tcggcgagta cttctacaca 6600 gccatcggtc cagacggccg cgcttctgcg ggcgatttgt gtacgcccga cagtcccggc 6660 tccggatcgg acgattgcgt cgcatcgacc ctgcgcccaa gctgcatcat cgaaattgcc 6720 gtcaaccaag ctctgataga gttggtcaag accaatgcgg agcatatacg cccggagccg 6780 cggcgatcct gcaagctccg gatgcctccg ctcgaagtag cgcgtctgct gctccataca 6840 agccaaccac ggcctccaga agaagatgtt ggcgacctcg tattgggaat ccccgaacat 6900 cgcctcgctc cagtcaatga ccgctgttat gcggccattg tccgtcagga cattgttgga 6960 gccgaaatcc gcgtgcacga ggtgccggac ttcggggcag tcctcggccc aaagcatcag 7020 ctcatcgaga gcctgcgcga cggacgcact gacggtgtcg tccatcacag tttgccagtg 7080 atacacatgg ggatcagcaa tcgcgcatat gaaatcacgc catgtagtgt attgaccgat 7140 tccttgcggt ccgaatgggc cgaacccgct cgtctggcta agatcggccg cagcgatcgc 7200 atccatggcc tccgcgaccg gctgcagaac agcgggcagt tcggtttcag gcaggtcttg 7260 caacgtgaca ccctgtgcac ggcgggagat gcaataggtc aggctctcgc tgaattcccc 7320 aatgtcaagc acttccggaa tcgggagcgc ggccgatgca aagtgccgat aaacataacg 7380 atctttgtag aaaccatcgg cgcagctatt tacccgcagg acatatccac gccctcctac 7440 atcgaagctg aaagcacgag attcttcgcc ctccgagagc tgcatcaggt cggagacgct 7500 gtcgaacttt tcgatcagaa acttctcgac agacgtcgcg gtgagttcag gctttttcat 7560 atctcattgc ccgggatctg cggcacgctg ttgacgctgt taagcgggtc gctgcagggt 7620 cgctcggtgt tcgaggccac acgcgtcacc ttaatatgcg aagtggacct gggaccgcgc 7680 cgccccgact gcatctgcgt gttcgaattc gccaatgaca agacgctggg cggggtttgt 7740 gtcatcatag aactaaagac atgcaaatat atttcttccg gggacaccgc cagcaaacgc 7800 gagcaacggg ccacggggat gaagcagggc atggcggccg acgcgctggg ctacgtcttg 7860 ctggcgttcg cgacgcgagg ctggatggcc ttccccatta tgattcttct cgcttccggc 7920 ggcatcggga tgcccgcgtt gcaggccatg ctgtccaggc aggtagatga cgaccatcag 7980 ggacagcttc aaggatcgct cgcggctctt accagcctaa cttcgatcac tggaccgctg 8040 atcgtcacgg cgatttatgc cgcctcggcg agcacatgga acgggttggc atggattgta 8100 ggcgccgccc tataccttgt ctgcctcccc gcgttgcgtc gcggtgcatg gagccgggcc 8160 acctcgacct gaatggaagc cggcggcacc tcgctaacgg attcaccact ccaagaattg 8220 gagccaatca attcttgcgg agaactgtga atgcgcaaac caacccttgg cagaacatat 8280 ccatcgcgtc cgccatctcc agcagccgca cgcggcgcag caaaaggcca ggaaccgtaa 8340 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 8400 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 8460 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 8520 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 8580 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 8640 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 8700 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 8760 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 8820 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 8880 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 8940 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 9000 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 9060 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 9120 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 9180 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 9240 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 9300 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 9360 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 9420 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 9480 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 9540 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 9600 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 9660 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 9720 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 9780 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 9840 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 9900 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 9960 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 10020 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 10080 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 10140 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa 10189 //