LOCUS       pGAD424-mABIN-2f4 7399 bp dsDNACircular
DEFINITION  LMBP 4394.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             422..829
                     /label=GALad
     promoter        8..411
                     /label=ADH1\promoter
     CDS             848..1600
                     /label=mABIN-2\(1-250)
     terminator      1981..2082
                     /label=ADH1\terminator
     CDS             complement(2286..3380)
                     /label=LEU2
     rep_origin      complement(4045..4447)
                     /label=pMB1\ori
     CDS             complement(4988..5848)
                     /label=AMP
     rep_origin      6419..7399
                     /label=2mu\ori
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga 
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac 
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct 
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga 
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa 
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg 
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt 
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc 
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag 
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc 
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata 
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg 
      841 gatccgtatg tcgtctgggg acccaaggtc tggtagacag gacggggccc cgcgtgcggc 
      901 cgcagcgctc tgtggcctgt accacgaggc cggccagcaa ctacagcgcc tgaaggatca 
      961 gctggccgcg cgtgacgccc tcatcgcgag cctccgcacc cgcctcgcgg ctctggaagg 
     1021 gcacacggcg ccgtcactcg tggacgcact tctggatcag gtggagcgct tccgtgagca 
     1081 gctgcgacga caggaggaag gcgcttcgga gacccagctg cggcaggaag ttgaaagact 
     1141 tacggagcgt ctagaggaaa aagagaggga gatgcaacag ctgatgagcc agcctcagca 
     1201 tgagcaagag aaggaggtag tcttgcttcg gcgaagtgtg gcagagaagg agaaagccag 
     1261 ggccgccagt gatgttctgt gccgctcctt ggctgatgag acccaccaac tgcgcaggac 
     1321 attggcagcc actgcccaca tgtgccaaca tctggccaaa tgtctggatg aacgacagtg 
     1381 tgcacaggga gacgctgggg agaaaagccc tgctgagcta gagcaaacaa gcagcgatgc 
     1441 ttctggccag agtgttatta agaagttaca ggaagaaaat cgactgttaa aacagaaggt 
     1501 gactcatgta gaagacctca atgctaagtg gcagcgttat gatgcaagta gggacgaata 
     1561 tgtgaagggg ttgcatgccc agctaaagag gcggcagtga agatctatga atcgtagata 
     1621 ctgaaaaacc ccgcaagttc acttcaactg tgcatcgtgc accatctcaa tttctttcat 
     1681 ttatacatcg ttttgccttc ttttatgtaa ctatactcct ctaagtttca atcttggcca 
     1741 tgtaacctct gatctataga attttttaaa tgactagaat taatgcccat cttttttttg 
     1801 gacctaaatt cttcatgaaa atatattacg agggcttatt cagaagcttt ggacttcttc 
     1861 gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt gccagaaatt 
     1921 tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa 
     1981 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta 
     2041 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttgttctt gagtaactct 
     2101 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc 
     2161 tctaccggca tgcccgaaat tcccctaccc tatgaacata ttccattttg taatttcgtg 
     2221 tcgtttctat tatgaatttc atttataaag tttatgtaca aatatcataa aaaaagagaa 
     2281 tctttttaag caaggatttt cttaacttct tcggcgacag catcaccgac ttcggtggta 
     2341 ctgttggaac cacctaaatc accagttctg atacctgcat ccaaaacctt tttaactgca 
     2401 tcttcaatgg ccttaccttc ttcaggcaag ttcaatgaca atttcaacat cattgcagca 
     2461 gacaagatag tggcgatagg gtcaacctta ttctttggca aatctggagc agaaccgtgg 
     2521 catggttcgt acaaaccaaa tgcggtgttc ttgtctggca aagaggccaa ggacgcagat 
     2581 ggcaacaaac ccaaggaacc tgggataacg gaggcttcat cggagatgat atcaccaaac 
     2641 atgttgctgg tgattataat accatttagg tgggttgggt tcttaactag gatcatggcg 
     2701 gcagaatcaa tcaattgatg ttgaaccttc aatgtaggaa attcgttctt gatggtttcc 
     2761 tccacagttt ttctccataa tcttgaagag gccaaaacat tagctttatc caaggaccaa 
     2821 ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt gattctttgc 
     2881 acttctggaa cggtgtattg ttcactatcc caagcgacac catcaccatc gtcttccttt 
     2941 ctcttaccaa agtaaatacc tcccactaat tctctgacaa caacgaagtc agtaccttta 
     3001 gcaaattgtg gcttgattgg agataagtct aaaagagagt cggatgcaaa gttacatggt 
     3061 cttaagttgg cgtacaattg aagttcttta cggattttta gtaaaccttg ttcaggtcta 
     3121 acactacctg taccccattt aggaccaccc acagcaccta acaaaacggc atcaaccttc 
     3181 ttggaggctt ccagcgcctc atctggaagt gggacacctg tagcatcgat agcagcacca 
     3241 ccaattaaat gattttcgaa atcgaacttg acattggaac gaacatcaga aatagcttta 
     3301 agaaccttaa tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg 
     3361 atcttcttag gggcagacat tagaatggta tatccttgaa atatatatat atattgctga 
     3421 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa 
     3481 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg 
     3541 aacgcttctc tattctatat gaaaagccgg ttccggcctc tcacctttcc tttttctccc 
     3601 aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc 
     3661 cagtcatcga atttgattct gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa 
     3721 aaaaataatg gttgctaaga gattcgaact cttgcatctt acgatacctg agtattccca 
     3781 cagttgggga tctcgactct agctagagga tcaattcgta atcatggtca tagctgtttc 
     3841 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     3901 gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc 
     3961 ccgctttcca gtcgggaaac ctgtcgtgcc agctggatta atgaatcggc caacgcgcgg 
     4021 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     4081 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     4141 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     4201 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     4261 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     4321 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     4381 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     4441 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     4501 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     4561 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     4621 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     4681 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     4741 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     4801 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     4861 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     4921 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     4981 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     5041 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     5101 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     5161 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     5221 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     5281 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     5341 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     5401 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     5461 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     5521 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     5581 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     5641 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     5701 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     5761 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     5821 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     5881 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     5941 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 
     6001 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 
     6061 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 
     6121 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 
     6181 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac cataacgcat 
     6241 ttaagcataa acacgcacta tgccgttctt ctcatgtata tatatataca ggcaacacgc 
     6301 agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg cattttcgga 
     6361 agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt ctctagctag 
     6421 aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca 
     6481 aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca 
     6541 caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc 
     6601 tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg 
     6661 tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga 
     6721 atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga 
     6781 agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca 
     6841 caaagtatgc gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa 
     6901 aatgcacccg cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt 
     6961 atggaagaga aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca 
     7021 aaaagttatc aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat 
     7081 gctttgttag aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag 
     7141 attctttgtt ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc 
     7201 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag 
     7261 cacagattct tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc 
     7321 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat 
     7381 gaagcacaga tgcttcgtt 
//
