LOCUS       pGAD424-mABIN-2d1 7876 bp dsDNACircular
DEFINITION  LMBP 4397.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     CDS             422..829
                     /label=GALad
     promoter        8..411
                     /label=ADH1\promoter
     CDS             848..2077
                     /label=mABIN-2\(d300-320)
     terminator      2458..2559
                     /label=ADH1\terminator
     CDS             complement(2763..3857)
                     /label=LEU2
     rep_origin      complement(4522..4924)
                     /label=pMB1\ori
     CDS             complement(5465..6325)
                     /label=AMP
     rep_origin      6896..7876
                     /label=2mu\ori
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga 
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac 
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct 
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga 
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa 
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg 
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt 
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc 
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag 
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc 
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata 
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg 
      841 gatccgtatg tcgtctgggg acccaaggtc tggtagacag gacggggccc cgcgtgcggc 
      901 cgcagcgctc tgtggcctgt accacgaggc cggccagcaa ctacagcgcc tgaaggatca 
      961 gctggccgcg cgtgacgccc tcatcgcgag cctccgcacc cgcctcgcgg ctctggaagg 
     1021 gcacacggcg ccgtcactcg tggacgcact tctggatcag gtggagcgct tccgtgagca 
     1081 gctgcgacga caggaggaag gcgcttcgga gacccagctg cggcaggaag ttgaaagact 
     1141 tacggagcgt ctagaggaaa aagagaggga gatgcaacag ctgatgagcc agcctcagca 
     1201 tgagcaagag aaggaggtag tcttgcttcg gcgaagtgtg gcagagaagg agaaagccag 
     1261 ggccgccagt gatgttctgt gccgctcctt ggctgatgag acccaccaac tgcgcaggac 
     1321 attggcagcc actgcccaca tgtgccaaca tctggccaaa tgtctggatg aacgacagtg 
     1381 tgcacaggga gacgctgggg agaaaagccc tgctgagcta gagcaaacaa gcagcgatgc 
     1441 ttctggccag agtgttatta agaagttaca ggaagaaaat cgactgttaa aacagaaggt 
     1501 gactcatgta gaagacctca atgctaagtg gcagcgttat gatgcaagta gggacgaata 
     1561 tgtgaagggg ttgcatgccc agctaaagag gcggcaggtc cctctggagc ctgagctgat 
     1621 gaagaaggag atttcccgac ttaacagaca gttggaggag aaaataagtg actgtgcgga 
     1681 agcaaaccag gagctgacag ccatgaggat gtcccgggac actgcgctgg agcgagtgca 
     1741 gatggcgcac agtaggattc aagagctgga ggaaaagatc atgtccttga tgtaccaagt 
     1801 gtcccagaga caggactccc gggagccagg accctgtcgg attcatacgg ggaacaaaac 
     1861 tgccaagtac ttagagatgg atgcactgga gcatgtgacc cctggcggct ggaggcctga 
     1921 gtctaggtcc caacagatgg aaccttctgc agagggtggg catgtgtgca cagcccagag 
     1981 aggtcagggt gaccttcagt gccctcattg cctgcggtgc ttcagtgatg agcaaggcga 
     2041 ggcattcctc aggcacctgt ctgagtgctg ccaatgaaga tctatgaatc gtagatactg 
     2101 aaaaaccccg caagttcact tcaactgtgc atcgtgcacc atctcaattt ctttcattta 
     2161 tacatcgttt tgccttcttt tatgtaacta tactcctcta agtttcaatc ttggccatgt 
     2221 aacctctgat ctatagaatt ttttaaatga ctagaattaa tgcccatctt ttttttggac 
     2281 ctaaattctt catgaaaata tattacgagg gcttattcag aagctttgga cttcttcgcc 
     2341 agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac 
     2401 gaaaagatgg aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg 
     2461 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac 
     2521 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tgttcttgag taactctttc 
     2581 ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct 
     2641 accggcatgc ccgaaattcc cctaccctat gaacatattc cattttgtaa tttcgtgtcg 
     2701 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct 
     2761 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg 
     2821 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct 
     2881 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac 
     2941 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat 
     3001 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc 
     3061 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg 
     3121 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca 
     3181 gaatcaatca attgatgttg aaccttcaat gtaggaaatt cgttcttgat ggtttcctcc 
     3241 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata 
     3301 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact 
     3361 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc 
     3421 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca 
     3481 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt 
     3541 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca 
     3601 ctacctgtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg 
     3661 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca 
     3721 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga 
     3781 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc 
     3841 ttcttagggg cagacattag aatggtatat ccttgaaata tatatatata ttgctgaaat 
     3901 gtaaaaggta agaaaagtta gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa 
     3961 taggtcctta aataatattg tcaacttcaa gtattgtgat gcaagcattt agtcatgaac 
     4021 gcttctctat tctatatgaa aagccggttc cggcctctca cctttccttt ttctcccaat 
     4081 ttttcagttg aaaaaggtat atgcgtcagg cgacctctga aattaacaaa aaatttccag 
     4141 tcatcgaatt tgattctgtg cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa 
     4201 aataatggtt gctaagagat tcgaactctt gcatcttacg atacctgagt attcccacag 
     4261 ttggggatct cgactctagc tagaggatca attcgtaatc atggtcatag ctgtttcctg 
     4321 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     4381 aagcctgggg tgcctaatga gtgaggtaac tcacattaat tgcgttgcgc tcactgcccg 
     4441 ctttccagtc gggaaacctg tcgtgccagc tggattaatg aatcggccaa cgcgcgggga 
     4501 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     4561 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     4621 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     4681 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     4741 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     4801 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     4861 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     4921 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     4981 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     5041 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     5101 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     5161 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     5221 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     5281 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     5341 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     5401 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     5461 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     5521 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     5581 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     5641 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     5701 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     5761 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     5821 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     5881 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     5941 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     6001 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     6061 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     6121 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     6181 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     6241 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     6301 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     6361 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     6421 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 
     6481 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg 
     6541 atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag 
     6601 cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg 
     6661 gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat aacgcattta 
     6721 agcataaaca cgcactatgc cgttcttctc atgtatatat atatacaggc aacacgcaga 
     6781 tataggtgcg acgtgaacag tgagctgtat gtgcgcagct cgcgttgcat tttcggaagc 
     6841 gctcgttttc ggaaacgctt tgaagttcct attccgaagt tcctattctc tagctagaaa 
     6901 gtataggaac ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa 
     6961 aaccaaaaac gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa 
     7021 acattgctca aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac 
     7081 ccatccacct ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt 
     7141 atctctagta ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc 
     7201 gaaaacaata cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga 
     7261 aaccgttcat aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa 
     7321 agtatgcgca atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat 
     7381 gcacccgcag cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg 
     7441 gaagagaaaa tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa 
     7501 agttatcaag agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct 
     7561 ttgttagaaa aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt 
     7621 ctttgttggt aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga 
     7681 ttctttgttt gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac 
     7741 agattcttcg ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc 
     7801 tcagattctt tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa 
     7861 gcacagatgc ttcgtt 
//
