LOCUS       pGAD424-mABIN-2m2AQ 7939 bp dsDNACircular
DEFINITION  LMBP 4402.


COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

FEATURES               Location/Qualifiers
     promoter        8..411
                     /label=ADH1\promoter
     CDS             422..829
                     /label=GALad
     terminator      2521..2622
                     /label=ADH1\terminator
     CDS             complement(2826..3920)
                     /label=LEU2
     rep_origin      complement(4585..4987)
                     /label=pMB1\ori
     CDS             complement(5528..6388)
                     /label=AMP
     rep_origin      6959..7939
                     /label=2mu\ori
     CDS             848..2140
                     /label=mABIN-2\m2AQ
ORIGIN
        1 gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc 
       61 atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga 
      121 cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac 
      181 tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct 
      241 tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga 
      301 cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt 
      361 ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa 
      421 gatggataaa gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg 
      481 taccgccgcc aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt 
      541 cactaacagt agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc 
      601 acaaccaatt gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag 
      661 taaaattgat gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc 
      721 gtataacgcg tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata 
      781 taactatcta ttcgatgatg aagatacccc accaaaccca aaaaaagaga tcgaattcgg 
      841 gatccgtatg tcgtctgggg acccaaggtc tggtagacag gacggggccc cgcgtgcggc 
      901 cgcagcgctc tgtggcctgt accacgaggc cggccagcaa ctacagcgcc tgaaggatca 
      961 gctggccgcg cgtgacgccc tcatcgcgag cctccgcacc cgcctcgcgg ctctggaagg 
     1021 gcacacggcg ccgtcactcg tggacgcact tctggatcag gtggagcgct tccgtgagca 
     1081 gctgcgacga caggaggaag gcgcttcgga gacccagctg cggcaggaag ttgaaagact 
     1141 tacggagcgt ctagaggaaa aagagaggga gatgcaacag ctgatgagcc agcctcagca 
     1201 tgagcaagag aaggaggtag tcttgcttcg gcgaagtgtg gcagagaagg agaaagccag 
     1261 ggccgccagt gatgttctgt gccgctcctt ggctgatgag acccaccaac tgcgcaggac 
     1321 attggcagcc actgcccaca tgtgccaaca tctggccaaa tgtctggatg aacgacagtg 
     1381 tgcacaggga gacgctgggg agaaaagccc tgctgagcta gagcaaacaa gcagcgatgc 
     1441 ttctggccag agtgttatta agaagttaca ggaagaaaat cgactgttaa aacagaaggt 
     1501 gactcatgta gaagacctca atgctaagtg gcagcgttat gatgcaagta gggacgaata 
     1561 tgtgaagggg ttgcatgccc agctaaagag gcggcaggtc cctctggagc ctgagctgat 
     1621 gaagaaggag atttcccgac ttaacagaca ggcgcaggag aaaataagtg actgtgcgga 
     1681 agcaaaccag gagctgacag ccatgaggat gtcccgggac actgcgctgg agcgagtgca 
     1741 gatgctagaa cagcagattc ttgcttacaa ggatgacttc aaatcagaaa gggcagatcg 
     1801 ggaacgagcg cacagtagga ttcaagagct ggaggaaaag atcatgtcct tgatgtacca 
     1861 agtgtcccag agacaggact cccgggagcc aggaccctgt cggattcata cggggaacaa 
     1921 aactgccaag tacttagaga tggatgcact ggagcatgtg acccctggcg gctggaggcc 
     1981 tgagtctagg tcccaacaga tggaaccttc tgcagagggt gggcatgtgt gcacagccca 
     2041 gagaggtcag ggtgaccttc agtgccctca ttgcctgcgg tgcttcagtg atgagcaagg 
     2101 cgaggcattc ctcaggcacc tgtctgagtg ctgccaatga agatctatga atcgtagata 
     2161 ctgaaaaacc ccgcaagttc acttcaactg tgcatcgtgc accatctcaa tttctttcat 
     2221 ttatacatcg ttttgccttc ttttatgtaa ctatactcct ctaagtttca atcttggcca 
     2281 tgtaacctct gatctataga attttttaaa tgactagaat taatgcccat cttttttttg 
     2341 gacctaaatt cttcatgaaa atatattacg agggcttatt cagaagcttt ggacttcttc 
     2401 gccagaggtt tggtcaagtc tccaatcaag gttgtcggct tgtctacctt gccagaaatt 
     2461 tacgaaaaga tggaaaaggg tcaaatcgtt ggtagatacg ttgttgacac ttctaaataa 
     2521 gcgaatttct tatgatttat gatttttatt attaaataag ttataaaaaa aataagtgta 
     2581 tacaaatttt aaagtgactc ttaggtttta aaacgaaaat tcttgttctt gagtaactct 
     2641 ttcctgtagg tcaggttgct ttctcaggta tagcatgagg tcgctcttat tgaccacacc 
     2701 tctaccggca tgcccgaaat tcccctaccc tatgaacata ttccattttg taatttcgtg 
     2761 tcgtttctat tatgaatttc atttataaag tttatgtaca aatatcataa aaaaagagaa 
     2821 tctttttaag caaggatttt cttaacttct tcggcgacag catcaccgac ttcggtggta 
     2881 ctgttggaac cacctaaatc accagttctg atacctgcat ccaaaacctt tttaactgca 
     2941 tcttcaatgg ccttaccttc ttcaggcaag ttcaatgaca atttcaacat cattgcagca 
     3001 gacaagatag tggcgatagg gtcaacctta ttctttggca aatctggagc agaaccgtgg 
     3061 catggttcgt acaaaccaaa tgcggtgttc ttgtctggca aagaggccaa ggacgcagat 
     3121 ggcaacaaac ccaaggaacc tgggataacg gaggcttcat cggagatgat atcaccaaac 
     3181 atgttgctgg tgattataat accatttagg tgggttgggt tcttaactag gatcatggcg 
     3241 gcagaatcaa tcaattgatg ttgaaccttc aatgtaggaa attcgttctt gatggtttcc 
     3301 tccacagttt ttctccataa tcttgaagag gccaaaacat tagctttatc caaggaccaa 
     3361 ataggcaatg gtggctcatg ttgtagggcc atgaaagcgg ccattcttgt gattctttgc 
     3421 acttctggaa cggtgtattg ttcactatcc caagcgacac catcaccatc gtcttccttt 
     3481 ctcttaccaa agtaaatacc tcccactaat tctctgacaa caacgaagtc agtaccttta 
     3541 gcaaattgtg gcttgattgg agataagtct aaaagagagt cggatgcaaa gttacatggt 
     3601 cttaagttgg cgtacaattg aagttcttta cggattttta gtaaaccttg ttcaggtcta 
     3661 acactacctg taccccattt aggaccaccc acagcaccta acaaaacggc atcaaccttc 
     3721 ttggaggctt ccagcgcctc atctggaagt gggacacctg tagcatcgat agcagcacca 
     3781 ccaattaaat gattttcgaa atcgaacttg acattggaac gaacatcaga aatagcttta 
     3841 agaaccttaa tggcttcggc tgtgatttct tgaccaacgt ggtcacctgg caaaacgacg 
     3901 atcttcttag gggcagacat tagaatggta tatccttgaa atatatatat atattgctga 
     3961 aatgtaaaag gtaagaaaag ttagaaagta agacgattgc taaccaccta ttggaaaaaa 
     4021 caataggtcc ttaaataata ttgtcaactt caagtattgt gatgcaagca tttagtcatg 
     4081 aacgcttctc tattctatat gaaaagccgg ttccggcctc tcacctttcc tttttctccc 
     4141 aatttttcag ttgaaaaagg tatatgcgtc aggcgacctc tgaaattaac aaaaaatttc 
     4201 cagtcatcga atttgattct gtgcgatagc gcccctgtgt gttctcgtta tgttgaggaa 
     4261 aaaaataatg gttgctaaga gattcgaact cttgcatctt acgatacctg agtattccca 
     4321 cagttgggga tctcgactct agctagagga tcaattcgta atcatggtca tagctgtttc 
     4381 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     4441 gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc 
     4501 ccgctttcca gtcgggaaac ctgtcgtgcc agctggatta atgaatcggc caacgcgcgg 
     4561 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     4621 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     4681 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     4741 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     4801 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     4861 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     4921 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     4981 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     5041 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     5101 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     5161 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg 
     5221 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     5281 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     5341 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     5401 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     5461 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     5521 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     5581 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     5641 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     5701 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     5761 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     5821 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     5881 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     5941 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     6001 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     6061 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     6121 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     6181 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     6241 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     6301 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     6361 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     6421 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     6481 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta 
     6541 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg 
     6601 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 
     6661 aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc 
     6721 ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac cataacgcat 
     6781 ttaagcataa acacgcacta tgccgttctt ctcatgtata tatatataca ggcaacacgc 
     6841 agatataggt gcgacgtgaa cagtgagctg tatgtgcgca gctcgcgttg cattttcgga 
     6901 agcgctcgtt ttcggaaacg ctttgaagtt cctattccga agttcctatt ctctagctag 
     6961 aaagtatagg aacttcagag cgcttttgaa aaccaaaagc gctctgaaga cgcactttca 
     7021 aaaaaccaaa aacgcaccgg actgtaacga gctactaaaa tattgcgaat accgcttcca 
     7081 caaacattgc tcaaaagtat ctctttgcta tatatctctg tgctatatcc ctatataacc 
     7141 tacccatcca cctttcgctc cttgaacttg catctaaact cgacctctac attttttatg 
     7201 tttatctcta gtattactct ttagacaaaa aaattgtagt aagaactatt catagagtga 
     7261 atcgaaaaca atacgaaaat gtaaacattt cctatacgta gtatatagag acaaaataga 
     7321 agaaaccgtt cataattttc tgaccaatga agaatcatca acgctatcac tttctgttca 
     7381 caaagtatgc gcaatccaca tcggtataga atataatcgg ggatgccttt atcttgaaaa 
     7441 aatgcacccg cagcttcgct agtaatcagt aaacgcggga agtggagtca ggcttttttt 
     7501 atggaagaga aaatagacac caaagtagcc ttcttctaac cttaacggac ctacagtgca 
     7561 aaaagttatc aagagactgc attatagagc gcacaaagga gaaaaaaagt aatctaagat 
     7621 gctttgttag aaaaatagcg ctctcgggat gcatttttgt agaacaaaaa agaagtatag 
     7681 attctttgtt ggtaaaatag cgctctcgcg ttgcatttct gttctgtaaa aatgcagctc 
     7741 agattctttg tttgaaaaat tagcgctctc gcgttgcatt tttgttttac aaaaatgaag 
     7801 cacagattct tcgttggtaa aatagcgctt tcgcgttgca tttctgttct gtaaaaatgc 
     7861 agctcagatt ctttgtttga aaaattagcg ctctcgcgtt gcatttttgt tctacaaaat 
     7921 gaagcacaga tgcttcgtt 
//
