ID pPICZTS standard; circular DNA; ; 5475 BP. DE LMBP 4420. CC Accession number: LMBP 4420 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 17-MAR-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 949..1191 FT /vntifkey="4" FT /label=ppMF FT /note="S. cerevisiae alpha-mating factor 1 prepro secretion signal sequence" FT misc_feature 773..937 FT /vntifkey="21" FT /label=5'\UTR\of\AOX1 FT /note="5' UTR of the P. pastoris AOX1 gene" FT promoter 7..772 FT /vntifkey="29" FT /label=AOX1\promoter FT /note="P. pastoris alcohol oxidase 1 promoter" FT rep_origin complement(4745..5025) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT terminator 4465..4735 FT /vntifkey="43" FT /label=CYC1\terminator FT /note="S. cerevisiae iso-1-cytochrome C terminator" FT CDS 4045..4419 FT /vntifkey="4" FT /label=zeocin FT /note="antibiotic resistance gene; coding sequence" FT promoter 3979..4044 FT /vntifkey="30" FT /label=synthetic\EM7\promoter FT /note="synthetic prokaryotic EM7 promoter" FT promoter 3565..3970 FT /vntifkey="29" FT /label=TEF1\promoter FT /note="S. cerevisiae translation elongation factor 1alpha promoter" FT terminator 3299..3558 FT /vntifkey="43" FT /label=AOX1\terminator FT /note="P. pastoris alcohol oxidase 1 terminator" FT misc_marker 3202..3219 FT /vntifkey="22" FT /label=His-tag FT /note="polyhistidine (6 x His) tag" FT misc_marker 3157..3186 FT /vntifkey="22" FT /label=Myc\epitope FT /note="Myc epitope" FT CDS 1219..3150 FT /vntifkey="4" FT /label=mature\TS FT /note="Trypanosoma cruzi trans-sialidase mature sequence" SQ Sequence 5475 BP; 1324 A; 1328 C; 1387 G; 1436 t; agatctaaca tccaaagacg aaaggttgaa tgaaaccttt ttgccatccg acatccacag 60 gtccattctc acacataagt gccaaacgca acaggagggg atacactagc agcagaccgt 120 tgcaaacgca ggacctccac tcctcttctc ctcaacaccc acttttgcca tcgaaaaacc 180 agcccagtta ttgggcttga ttggagctcg ctcattccaa ttccttctat taggctacta 240 acaccatgac tttattagcc tgtctatcct ggcccccctg gcgaggttca tgtttgttta 300 tttccgaatg caacaagctc cgcattacac ccgaacatca ctccagatga gggctttctg 360 agtgtggggt caaatagttt catgttcccc aaatggccca aaactgacag tttaaacgct 420 gtcttggaac ctaatatgac aaaagcgtga tctcatccaa gatgaactaa gtttggttcg 480 ttgaaatgct aacggccagt tggtcaaaaa gaaacttcca aaagtcgcca taccgtttgt 540 cttgtttggt attgattgac gaatgctcaa aaataatctc attaatgctt agcgcagtct 600 ctctatcgct tctgaacccc ggtgcacctg tgccgaaacg caaatgggga aacacccgct 660 ttttggatga ttatgcattg tctccacatt gtatgcttcc aagattctgg tgggaatact 720 gctgatagcc taacgttcat gatcaaaatt taactgttct aacccctact tgacagcaat 780 atataaacag aaggaagctg ccctgtctta aacctttttt tttatcatca ttattagctt 840 actttcataa ttgcgactgg ttccaattga caagcttttg attttaacga cttttaacga 900 caacttgaga agatcaaaaa acaactaatt attcgaagga tccaaacgat gagatttcct 960 tcaattttta ctgcagtttt attcgcagca tcctccgcat tagctgctcc agtcaacact 1020 acaacagaag atgaaacggc acaaattccg gctgaagctg tcatcggtta ctcagattta 1080 gaaggggatt tcgatgttgc tgttttgcca ttttccaaca gcacaaataa cgggttattg 1140 tttataaata ctactattgc cagcattgct gctaaagaag aaggggtatc tctcgagaaa 1200 agagaggctg aagcttacgt gctggcaccc ggatcgagcc gagttgagct gtttaagcgt 1260 aagaattcga cggtgccgtt tgaagacaag gccggcaaag tcaccgagcg ggttgtccac 1320 tcgttccgcc tccccgccct tgttaatgtg gacggggtga tggttgccat cgcggacgct 1380 cgctacgaca catccaatga caactccctc attgatacgg tggcgaagta cagcgtggac 1440 gatggggaga cgtgggagac ccaaattgcc atcaagaaca gccgtgtatc gtctgtttct 1500 cgtgtggtgg atcccaccgt gattgtgaag ggcaacaagc tttacgtcct ggttggaagc 1560 tactatagtt cgagaagcta ctggtcgtcg catggtgatg cgagagactg ggatattctg 1620 cttgccgttg gtgaggtcac gaagtccatt gcgggcggca agataactgc gagtatcaaa 1680 tgggggagcc ccgtgtcact gaagaagttt tttccggcag aaatggaagg catgcacaca 1740 aatcaatttc ttggcggcgc gggtgttgcc attgtggcgt ccaacgggaa tcttgtgtac 1800 cctgtgcagg ttacgaacaa aaggaagcaa gttttctcca agatcttcta ctcggaagat 1860 gatggcaaga cgtggaagtt tgggaagggt aggagcgatt ttggctgctc tgaacctgtg 1920 gcccttgagt gggaggggaa gctcatcata aacacccgag ttgactggaa acgccgtctg 1980 gtgtacgagt ccagtgacat ggggaatacg tgggtggagg ctgtcggcac gctctcgcgt 2040 gtgtggggcc cctcaccaaa atcggaccag cccggcagtc agagcagctt cactgccgtg 2100 accatcgaag gaatgcgtgt gatgctcttc acacacccgc tgaattttaa gggaaggtgg 2160 ctgcgcgacc gactgaacct ctggctgacg gataaccagc gcatttataa cgttgggcaa 2220 gtatccattg gtgatgaaaa ttccgcctac agctccgtcc tgtacaagga tgataagctg 2280 tactgtttgc atgagatcaa cacggacgag gtgtacagcc ttgtttttgc acgcctggtt 2340 ggcgagctac ggatcattaa atcagtgctg cgatcctgga agaattggga cagccacctg 2400 tccagcattt gcacccctgc tgatccagcc gcttcgtcgt cagagagtgg ttgtggtccc 2460 gctgtcacca cggttggtct tgttggcttt ttgtccggca atgcctccca aaacgtatgg 2520 gaggatgcgt accgctgcgt caacgcaagc acggcaaatg cggagagggt tcggaacggt 2580 ttgaagtttg cgggggttgg cggaggggcg ctttggccgg tgagccagca ggggcagaat 2640 cagcggtatc gttttgcaaa ccacgcgttc acgctggtgg cgtcggtgac gattcacgag 2700 gctccgaggg ccgcgagtcc tttgctgggt gcgagcctgg actcttctgg tggcaaaaaa 2760 ctcctggggc tctcgtacga cgagaagcac cagtggcagc caatatacgg atcaacgccg 2820 gtgacgccga cgggatcgtg ggagacgggt aagaggtacc acgtggttct tacggtggcg 2880 aataaaattg gctccgtgta cattgatgga gaacttctgg agggttcagg acagaccgtt 2940 gtgccagacg ggaggacgcc tgacatctcc cacttctacg ttggcgggta tggaaggagt 3000 gatatgccaa ccataagcca cgtgacggtg aataatgttc ttctttacaa ccgacagctg 3060 aataccgagg agatcaggac cttgttcttg agccaggacc tgattggcac ggaagcacac 3120 atggacagca gcagcgacac gaaggaattg ggtctagaac aaaaactcat ctcagaagag 3180 gatctgaata gcgccgtcga ccatcatcat catcatcatt gagtttgtag ccttagacat 3240 gactgttcct cagttcaagt tgggcactta cgagaagacc ggtcttgcta gattctaatc 3300 aagaggatgt cagaatgcca tttgcctgag agatgcaggc ttcatttttg atactttttt 3360 atttgtaacc tatatagtat aggatttttt ttgtcatttt gtttcttctc gtacgagctt 3420 gctcctgatc agcctatctc gcagctgatg aatatcttgt ggtaggggtt tgggaaaatc 3480 attcgagttt gatgtttttc ttggtatttc ccactcctct tcagagtaca gaagattaag 3540 tgagaccttc gtttgtgcgg atcccccaca caccatagct tcaaaatgtt tctactcctt 3600 ttttactctt ccagattttc tcggactccg cgcatcgccg taccacttca aaacacccaa 3660 gcacagcata ctaaattttc cctctttctt cctctagggt gtcgttaatt acccgtacta 3720 aaggtttgga aaagaaaaaa gagaccgcct cgtttctttt tcttcgtcga aaaaggcaat 3780 aaaaattttt atcacgtttc tttttcttga aatttttttt tttagttttt ttctctttca 3840 gtgacctcca ttgatattta agttaataaa cggtcttcaa tttctcaagt ttcagtttca 3900 tttttcttgt tctattacaa ctttttttac ttcttgttca ttagaaagaa agcatagcaa 3960 tctaatctaa ggggcggtgt tgacaattaa tcatcggcat agtatatcgg catagtataa 4020 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc 4080 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac 4140 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg 4200 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac 4260 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg 4320 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc 4380 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtccgacg gcggcccacg 4440 ggtcccaggc ctcggagatc cgtccccctt ttcctttgtc gatatcatgt aattagttat 4500 gtcacgctta cattcacgcc ctccccccac atccgctcta accgaaaagg aaggagttag 4560 acaacctgaa gtctaggtcc ctatttattt ttttatagtt atgttagtat taagaacgtt 4620 atttatattt caaatttttc ttttttttct gtacagacgc gtgtacgcat gtaacattat 4680 actgaaaacc ttgcttgaga aggttttggg acgctcgaag gctttaattt gcaagctgga 4740 gaccaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 4800 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 4860 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 4920 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 4980 gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 5040 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 5100 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 5160 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 5220 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 5280 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 5340 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 5400 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 5460 tttggtcatg agatc 5475 //