LOCUS       pUC21:synthCandidaBAX        3773 bp    DNA     circular     10-APR-2006
DEFINITION  LMBP 4436.
SOURCE      
  ORGANISM  
COMMENT     (800)357-3114 or http://www.informaxinc.com/
            
COMMENT     VNTDATE|407323575|
COMMENT     VNTNAME|pUC21:synthCandidaBAX|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
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COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 867 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1276 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1499 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 884 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 845 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "XhoI" "ctcgag" 2 0 1 1520 0 0 "")) 
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COMMENT        #10=(CFSignal (CObList) "pMB1 ori" 33 0 1 1770 2172 1 (CStringList) 
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COMMENT        #11=(CFSignal (CObList) "lac promoter with SacII" 30 0 1 1593 1678 1 
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COMMENT        #13=(CFSignal (CObList) "AMP" 4 0 1 2713 3573 1 (CStringList) 
COMMENT             (CStringList "/gene=\"bla\"" "/codon_start=1" "/transl_table=11" 
COMMENT              "/product=\"beta-lactamase\"" "/protein_id=\"AAF71714.1\"" 
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COMMENT                          "Created: 04/10/06 09:26" 1) 
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COMMENT                          "Last Modified: 04/10/06 09:31" 1) 
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FEATURES             Location/Qualifiers
     promoter        800..816
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      187..564
                     /vntifkey="33"
                     /label=M13d\ori
     CDS             908..1486
                     /vntifkey="4"
                     /label=synth\CaBAX
     rep_origin      complement(1770..2172)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(1593..1678)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             1555..1592
                     /vntifkey="32"
                     /label=lacZ\RBS
     CDS             complement(2713..3573)
                     /label=AMP
BASE COUNT      962 a       904 c       963 g       944 t 
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 
      181 accataaaat tgtaaacgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca 
      241 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaatagc 
      301 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg 
      361 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccaccc cgatttagag 
      421 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg 
      481 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc 
      541 ttaatgcgcc gctacagggc gcgtactatg gttgctttga cgtatgcggt gtgaaatacc 
      601 gcacagatgc gtaaggagaa aataccgcat caggcgccat tcgccattca ggctgcgcaa 
      661 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg 
      721 atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa 
      781 aacgacggcc agtgaattgt aatacgactc actatagggc gaattgggga tcgatccact 
      841 agttctagag cggccgccac ggcgatatcg gatccatatg acgtcgacgc gtctgcagaa 
      901 gatcttcatg gatggttctg gtgaacaatt gggttctggt ggtccaacct cttctgaaca 
      961 aatcatgaaa accggtgctt tcttgttgca aggtttcatc caagatagag ctggtagaat 
     1021 ggctggtgaa accccagaat tgaccttgga acaaccacca caagatgctt ctaccaaaaa 
     1081 attgtctgaa tgtttgagaa gaatcggtga tgaattggat tctaacatgg aattgcaaag 
     1141 aatgatcgct gatgtcgata ccgattctcc aagagaagtc ttcttcagag tcgctgctga 
     1201 tatgttcgct gatggtaact tcaactgggg tagagtcgtc gctttgttct acttcgcttc 
     1261 taaattggtc ttgaaagctt tgtgtaccaa agtcccagaa ttgatcagaa ccatcatggg 
     1321 ttggaccttg gatttcttga gagaaagatt gttggtctgg atccaagatc aaggtggttg 
     1381 ggaaggtttg ttgtcttact tcggtacccc aacctggcaa accgtcacca tcttcgtcgc 
     1441 tggtgtcttg accgcttctt tgaccatctg gaaaaaaatg ggttaatccc ccgggtacca 
     1501 tggcatgcat cgatagatct cgaggcctcg gactagtggc gtaatcatgg tcatagctgt 
     1561 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc gcggaagcat 
     1621 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc 
     1681 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg 
     1741 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct 
     1801 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt 
     1861 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc 
     1921 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga 
     1981 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata 
     2041 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac 
     2101 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg 
     2161 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc 
     2221 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag 
     2281 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt 
     2341 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt 
     2401 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg 
     2461 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac 
     2521 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca 
     2581 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac 
     2641 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac 
     2701 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt 
     2761 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt 
     2821 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt 
     2881 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc 
     2941 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa 
     3001 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg 
     3061 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt 
     3121 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc 
     3181 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt 
     3241 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg 
     3301 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac 
     3361 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc 
     3421 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt 
     3481 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg 
     3541 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag 
     3601 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa 
     3661 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat 
     3721 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtc 
//
