LOCUS       pGAL1P:synthCaBAX        5946 bp    DNA     circular     15-OCT-2013
DEFINITION  LMBP 4439.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4439
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-OCT-2013

COMMENT     VNTDATE|548851131|
COMMENT     VNTNAME|pGAL1P:synthCaBAX|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pGAL1P:synthCaBAX" 0 5946 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 150 0 
COMMENT        1 0 (CHomObj 1 0 0 3 75) 
COMMENT        (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) (CWordArray) 
COMMENT        (CStringList "BamHI" "EcoRI" "HindIII" "PstI" "SmaI" "StuI" "Bpu1102I") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 5476 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 4 311 0 1056 0 4138 0 5097 
COMMENT            0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 651 0 5560 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 1 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SmaI" "cccggg" 4 0 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "StuI" "aggcct" 4 0 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "Bpu1102I" "gctnagc" 3 0 1 971 0 0 "")) 
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COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 2216 3076 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2216..3076)") 
COMMENT        #9=(CFSignal (CObList) "Candida GAL1 promoter" 29 0 1 2 1214 0 
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COMMENT        #10=(CFSignal (CObList) "Candida Wh11 terminator" 43 0 1 5203 5337 0 
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COMMENT        #11=(CFSignal (CObList) "Candida BAX (codon adapted mouse BAX)" 4 0 1 
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COMMENT                          "DNA Plasmid 'pGAL1P:synthCaBAX'" 1) 
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COMMENT                          "Created: 03/16/10 10:38" 1) 
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COMMENT                          "Last Modified: 10/15/13 03:40" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5946 bp" 1) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.lmbp@dmbr.ugent.be\">bccm.lmbp@dmbr.ugent.be</a>" 
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FEATURES             Location/Qualifiers
     rep_origin      complement(1267..1675)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(2216..3076)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(2..1214)
                     /vntifkey="29"
                     /label=Candida\GAL1\promoter
     terminator      complement(5203..5337)
                     /vntifkey="43"
                     /label=Candida\Wh11\terminator
     CDS             complement(5353..5931)
                     /vntifkey="4"
                     /label=Candida\BAX\(codon\adapted\mouse\BAX)
     CDS             4005..4817
                     /vntifkey="4"
                     /label=Candida\URA3
BASE COUNT     1821 a      1214 c      1222 g      1689 t 
ORIGIN
        1 gggtataact ctttcttata aaaatcggtt tgtatgtgtg tatatgtggg agaaaaaaaa 
       61 atttacaaag aaatcaaaaa ggatctggga aactttcaaa ctctatttta tatcaaagtt 
      121 taattttata tgattgtaat aacaagtggg caaattcttt caaaactatt gaatgaagaa 
      181 agaaaaccaa tgagctaaaa cattctttcc aaaaaaaaag aaaaacgaaa actaaacacc 
      241 aaccccggaa accgaactat catgaatcgt gtcagatgtc taaacattgt gtgaagatga 
      301 gagaggaatg aattctccag acacaaacaa acaccaaaaa ttttaataat ccttgttcag 
      361 tttttctttg cccgacaaaa aaaataaacc taaaaaaaat tttcatttcg gaaaaatcat 
      421 aaatcgcttt tcaccttcaa ataaacataa attgtaacgt tacatttaac tttctcataa 
      481 ttaattatga ttttggtgtc gggaaaatac aatcatcttt gtccatatat tttttggaag 
      541 gttttgtgaa ctgcttaaaa ttttttcccc cccactttcc ccgatggaat gaaaacataa 
      601 attaatccga agattcccca aaattttttt cggacactgg ccacaaaaca agcttttttt 
      661 cttggcaata ttttttacac atagcacaaa actaggctga caaaaaaaca gaactaaaac 
      721 aatggagaaa atagtgttcc cacttgtatt ctgttcgtgg ccaagtactc ctctatgttc 
      781 gcttctaatt cccataatta ctttatgtgt ttgtgtgctg gtcatccaat tccagtttca 
      841 ttattttcaa aatgaaatgt atcaaccggt ttgcattgtt atttggttag ttgactaata 
      901 cctcacatta ccaatttttg aaactcaatt tctgtctggt ttttcaagca tcatttctta 
      961 tatttagagc taagcctaaa aatatcttct gatcatccat ccaaacgttt ttgtgcacag 
     1021 tctggagtaa acaaacaatc tagacatcca ataagaattc ccattctcta aaaaaaacac 
     1081 gtttcgtttg gaattaaacc aagggaaatg atattcaaaa attattttca cacttttaca 
     1141 ccgtttcttc ccgtttattg tgtgttttct tcagtgtaac gttacattgg ttcctcttcg 
     1201 tcaatatcag atctctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta 
     1261 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc 
     1321 gagcggtatc agatcgatct cactcaaagg cggtaatacg gttatccaca gaatcagggg 
     1381 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 
     1441 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 
     1501 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 
     1561 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 
     1621 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 
     1681 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 
     1741 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 
     1801 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 
     1861 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 
     1921 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 
     1981 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 
     2041 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 
     2101 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 
     2161 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 
     2221 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 
     2281 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 
     2341 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 
     2401 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 
     2461 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 
     2521 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 
     2581 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 
     2641 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 
     2701 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 
     2761 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 
     2821 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 
     2881 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 
     2941 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 
     3001 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 
     3061 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 
     3121 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 
     3181 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 
     3241 cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg 
     3301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg 
     3361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggctggctta 
     3421 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc 
     3481 acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg ctgcgcaact 
     3541 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagactaat aggaattgat 
     3601 ttggatggta taaacggaaa caaaaaaaag agctggtact actttcttta aaattatttt 
     3661 attatttgat tttatttaat agtatatatt atattttgaa cgtagattat tttgttgaaa 
     3721 gttgctgtag tgccattgat tcgtaacact aattctgtat tagtcattcc tcttgtttga 
     3781 tagtatccaa aaaaacggct atttttttgc aatcttattt cctgcatatt atacagataa 
     3841 cataatgaaa gaaaaaatct ttttttttgt tcttcaatga tgatttcaac cattctttta 
     3901 aacattgatc aattcctgag caacaacccc atacacactg gtttatatat cgcccctttt 
     3961 acagttgaag aaagaaatag aaatagaaat agcaaacaaa agatatgaca gtcaacacta 
     4021 agacctatag tgagagagca gaaactcatg cctcaccagt agcacaacga ttatttcgat 
     4081 taatggaact gaagaaaacc aatttatgtg catcaattga tgttgatacc actaaggaat 
     4141 tccttgaatt aattgataaa ttgggtcctt atgtatgctt aatcaagact catattgata 
     4201 taatcaatga tttttcctat gaatccacta ttgaaccatt attagaactt tcacgtaaac 
     4261 atcaatttat gatttttgaa gatagaaaat ttgctgatat tggtaatacc gtgaagaaac 
     4321 aatatattgg tggagtttat aaaattagta gttgggcaga tattactaat gctcatggtg 
     4381 tcactgggaa tggagtagtt gaaggattaa aacagggagc taaagaaacc accaccaacc 
     4441 aagagccaag agggttattg atgttagctg aattatcatc agtgggatca ttagcatatg 
     4501 gagaatattc tcaaaaaact gttgaaattg ctaaatccga taaggaattt gttattggat 
     4561 ttattgccca acgtgatatg ggtggacaag aagaaggatt tgattggctt attatgacac 
     4621 ctggagttgg attagatgat aaaggtgatg gattaggaca acaatataga actgttgatg 
     4681 aagttgttag cactggaact gatattatca ttgttggtag aggattgttt ggtaaaggaa 
     4741 gagatccaga tattgaaggt aaaaggtata gagatgctgg ttggaatgct tatttgaaaa 
     4801 aaactggcca attataaatg tgaaggggga gattttcact ttattagatt tgtatatatg 
     4861 tataataaat aaataaataa gttaaataaa taattaaata agggtggtaa ttattactat 
     4921 ttacaatcaa aggtggtcct tctagctgta atccgggcag cgcaacggaa cattcatcag 
     4981 tgtaaaaatg gaatcaataa agccctgcct ggcgaaaggg ggatgtgctg caaggcgatt 
     5041 aagttgggta acgccagggt tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt 
     5101 cgagctcgca tggtgagacg cgacagacac cacacacttg gtagcaatca tcggtattcc 
     5161 ttgtatttgc cccacacaaa tctacaaatt tgacattaat taatagcttc acatttatta 
     5221 aagaatataa aataaactaa agaatattaa aaaaaaacgt tataaaaata gatagctcaa 
     5281 tggctcatct aagcgaaaca aaatcatagc aagacttcaa ctcaattgag gttactcggg 
     5341 cccagggggg gattaaccca tttttttcca gatggtcaaa gaagcggtca agacaccagc 
     5401 gacgaagatg gtgacggttt gccaggttgg ggtaccgaag taagacaaca aaccttccca 
     5461 accaccttga tcttggatcc agaccaacaa tctttctctc aagaaatcca aggtccaacc 
     5521 catgatggtt ctgatcaatt ctgggacttt ggtacacaaa gctttcaaga ccaatttaga 
     5581 agcgaagtag aacaaagcga cgactctacc ccagttgaag ttaccatcag cgaacatatc 
     5641 agcagcgact ctgaagaaga cttctcttgg agaatcggta tcgacatcag cgatcattct 
     5701 ttgcaattcc atgttagaat ccaattcatc accgattctt ctcaaacatt cagacaattt 
     5761 tttggtagaa gcatcttgtg gtggttgttc caaggtcaat tctggggttt caccagccat 
     5821 tctaccagct ctatcttgga tgaaaccttg caacaagaaa gcaccggttt tcatgatttg 
     5881 ttcagaagag gttggaccac cagaacccaa ttgttcacca gaaccatcca tgatgatctt 
     5941 cctgca 
//
