ID pLT-mCASP-1p30 standard; circular DNA; ; 4541 BP. DE LMBP 4457. CC Accession number: LMBP 4457 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 31-MAY-2010 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 24..40 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT misc_feature 41..103 FT /vntifkey="21" FT /label=5'\UTR\of\T7\gene\10,\incl.\RBS FT /note="5' UTR of phage T7 gene 10, incl. ribosome binding site" FT promoter complement(1136..1152) FT /vntifkey="30" FT /label=T3\promoter FT terminator 1170..1217 FT /vntifkey="43" FT /label=T7\terminator FT /note="phage T7 gene 10 terminator" FT terminator 1291..1338 FT /vntifkey="43" FT /label=T7\terminator FT /note="phage T7 gene 10 terminator" FT terminator 1407..1488 FT /vntifkey="43" FT /label=fd\terminator FT /note="phage fd terminator" FT terminator 1517..1598 FT /vntifkey="43" FT /label=fd\terminator FT /note="phage fd terminator" FT rep_origin 1696..2151 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT rep_origin complement(2155..2528) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(3069..3929) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT promoter 4295..4425 FT /vntifkey="30" FT /label=PL\promoter FT /note="phage lambda major leftward promoter" FT CDS 104..949 FT /vntifkey="4" FT /label=mCASP-1p30 FT misc_feature 950..1061 FT /vntifkey="21" FT /label=3'\UTR\of\mCASP-1 SQ Sequence 4541 BP; 1244 A; 1064 C; 1044 G; 1189 t; ttccggatct cgatcccgga aattaatacg actcactata gggagaccac aacggtttcc 60 ctctagaaat aattttgttt aactttaaga aggagatata catatgggca catttccagg 120 actgactggg accctcaagt tttgcccttt agaaaaagcc cagaagttat ggaaagaaaa 180 tccttcagag atttatccaa taatgaatac aaccactcgt acacgtcttg ccctcattat 240 ctgcaacaca gagtttcaac atctttctcc gagggttgga gctcaagttg acctcagaga 300 aatgaagttg ctgctggagg atctggggta taccgtgaaa gtgaaagaaa atctcacagc 360 tctggagatg gtgaaagagg tgaaagaatt tgctgcctgc ccagagcaca agacttctga 420 cagtactttc cttgtattca tgtctcatgg tatccaggag ggaatatgtg ggaccacata 480 ctctaatgaa gtttcagata ttttaaaggt tgacacaatc tttcagatga tgaacacttt 540 gaagtgccca agcttgaaag acaagcccaa ggtgatcatt attcaggcat gccgtggaga 600 gaaacaagga gtggtgttgt taaaagattc agtaagagac tctgaagagg atttcttaac 660 ggatgcaatt tttgaagatg atggcattaa gaaggcccat atagagaaag attttattgc 720 tttctgctct tcaacaccag ataatgtgtc ttggagacat cctgtcaggg gctcactttt 780 cattgagtca ctcatcaaac acatgaaaga atatgcctgg tcttgtgact tggaggacat 840 tttcagaaag gttcgatttt catttgaaca accagaattt aggctacaga tgcccactgc 900 tgatagggtg accctgacaa aacgtttcta cctcttcccg ggacattaaa cgaagaatcc 960 agttcattct tatgtaccta tgctgagaat cgtgccaata agaagccaat acttccttag 1020 atgatgcaat aaatattaaa ataaaacaaa acagaaaggc taaaaaaaaa aaaaaaaagc 1080 tccgcccgga tccctcgagg tcgacgaatt cgagctcggc cgacttggcc ttccctttag 1140 tgagggttaa taaacttggt gagcaataac tagcataacc ccttggggcc tctaaacggg 1200 tcttgagggg ttttttgctg aaaggaggaa ctatatgcgc tcatacgata tgaacgttga 1260 gactgccgct gagttatcag tgagcaataa ctagcataac cccttggggc ctctaaacgg 1320 gtcttgaggg gttttttgct gaaaggagga actatatccg gatagcttat cgctagaggt 1380 cgaaattcac ctcgaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc 1440 tttttttttg gagattttca acgtgaaaaa attattattc gcaattccaa gctaattcac 1500 ctcgaaagca agctgataaa ccgatacaat taaaggctcc ttttggagcc tttttttttg 1560 gagattttca acgtgaaaaa attattattc gcaattccaa gctctgcctc gcgcgtttcg 1620 gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt 1680 aagcggatgc agatcacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta 1740 cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc 1800 cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt 1860 tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg 1920 gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca 1980 cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct 2040 attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga 2100 tttaacaaaa atttaacgcg aattttaaca aaatattaac gtttacaatt tgatctgcgc 2160 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc 2220 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg 2280 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 2340 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 2400 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 2460 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 2520 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 2580 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 2640 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 2700 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt 2760 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 2820 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 2880 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 2940 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 3000 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 3060 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 3120 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 3180 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 3240 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 3300 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 3360 ttgcgcaacg ttgttgccat tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg 3420 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 3480 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 3540 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 3600 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 3660 ccgagttgct cttgcccggc gtcaacacgg gataataccg cgccacatag cagaacttta 3720 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 3780 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 3840 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 3900 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta tgtaagcaga 3960 cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg 4020 agacacaacg tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagatcac 4080 gcatcttccc gacaacgcag accgttccgt ggcaaagcaa aagttcaaaa tcaccaactg 4140 gtccacctac aacaaagctc tcatcaaccg tggctccctc actttctggc tggatgatgg 4200 ggcgattcag gcctggtatg agtcagcaac accttcttca cgaggcagac ctcagcgccg 4260 gtgatgccgg ccacgatgcg tccggcgtag aggatctctc acctaccaaa caatgccccc 4320 ctgcaaaaaa taaattcata taaaaaacat acagataacc atctgcggtg ataaattatc 4380 tctggcggtg ttgacataaa taccactggc ggtgatactg agcacatcag caggacgcac 4440 tgaccaccat gaaggtgacg ctcttaaaat taagccctga agaagggcag cattcaaagc 4500 agaaggcttt ggggtgtgtg atacgaaacg aagcattgga a 4541 //