LOCUS       pTRKH3                  7766 bp    DNA     circular     25-OCT-2011
DEFINITION  LMBP 4462.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4462
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 25-OCT-2011
            
COMMENT     VNTNAME|pTRKH3|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
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COMMENT        #1=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 4846 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 6527 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 3868 0 6369 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "AvaI" "cycgrg" 2 0 1 7765 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1935 0 0 "")) 
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COMMENT        #7=(CFSignal (CObList) "pAMB1 ori" 33 0 0 1935 4850 0 (CStringList) 
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COMMENT        #8=(CFSignal (CObList) "p15A ori" 33 0 1 5617 6116 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "tetracycline resistance" 4 0 0 6425 7615 0 
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COMMENT        #10=(CFSignal (CObList) "repE" 4 0 0 2864 4354 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pTRKH3'" 1) 
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COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7766 bp" 1) 
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FEATURES             Location/Qualifiers
     CDS             543..1280
                     /vntifkey="4"
                     /label=erythromycin\resistance
     rep_origin      1935..4850
                     /vntifkey="33"
                     /label=pAMB1\ori
                     /note="part of pAMbeta1 containing replication origin, incl. repD and repE"
     rep_origin      complement(5617..6116)
                     /vntifkey="33"
                     /label=p15A\ori
                     /note="E. coli p15A origin"
     CDS             6425..7615
                     /vntifkey="4"
                     /label=tetracycline\resistance
     CDS             2864..4354
                     /vntifkey="4"
                     /label=repE
     CDS             2522..2821
                     /vntifkey="4"
                     /label=repD
BASE COUNT     2433 a      1527 c      1683 g      2123 t 
ORIGIN
        1 gagtctagaa tcgatacgat tttgaagtgg caacagataa aaaaaagcag tttaaaattg 
       61 ttgctgaact tttaaaacaa gcaaatacaa tcattgtcgc aacagatagc gacagagaag 
      121 gcgaaaacat tgcctggtcg atcattcata aagcaaatgc cttttctaaa gataaaacgt 
      181 ataaaagact atggatcaat agtttagaaa aagatgtgat ccgtagcggt tttcaaaatt 
      241 tgcaaccagg aatgaattac tatccctttt atcaagaagc gcaaaagaaa aacgaaatga 
      301 tacaccaatc agtgcaaaaa aagatataat gggagataag acggttcgtg ttcgtgctga 
      361 cttgcaccat atcataaaaa tcgaaacagc aaagaatggc ggaaacgtaa aagaagttat 
      421 ggaaataaga cttagaagca aacttaagag tgtgttgata gtgcagtatc ttaaaatttt 
      481 gtataatagg aattgaagtt aaattagatg ctaaaaattt gtaattaaga aggagtgatt 
      541 acatgaacaa aaatataaaa tattctcaaa actttttaac gagtgaaaaa gtactcaacc 
      601 aaataataaa acaattgaat ttaaaagaaa ccgataccgt ttacgaaatt ggaacaggta 
      661 aagggcattt aacgacgaaa ctggctaaaa taagtaaaca ggtaacgtct attgaattag 
      721 acagtcatct attcaactta tcgtcagaaa aattaaaact gaatactcgt gtcactttaa 
      781 ttcaccaaga tattctacag tttcaattcc ctaacaaaca gaggtataaa attgttggga 
      841 gtattcctta ccatttaagc acacaaatta ttaaaaaagt ggtttttgaa agccatgcgt 
      901 ctgacatcta tctgattgtt gaagaaggat tctacaagcg taccttggat attcaccgaa 
      961 cactagggtt gctcttgcac actcaagtct cgattcagca attgcttaag ctgccagcgg 
     1021 aatgctttca tcctaaacca aaagtaaaca gtgtcttaat aaaacttacc cgccatacca 
     1081 cagatgttcc agataaatat tggaagctat atacgtactt tgtttcaaaa tgggtcaatc 
     1141 gagaatatcg tcaactgttt actaaaaatc agtttcatca agcaatgaaa cacgccaaag 
     1201 taaacaattt aagtaccgtt acttatgagc aagtattgtc tatttttaat agttatctat 
     1261 tatttaacgg gaggaaataa ttctatgagt cgcttttgta aatttggaaa gttacacgtt 
     1321 actaaaggga atgtagataa attattaggt atactactga cagcttccaa ggagctaaag 
     1381 aggtccctag cgctcttatc atggggaagc tcggatcata tgcaagacaa aataaactcg 
     1441 caacagcact tggagaaatg ggacgaatcg agaaaaccct ctttacgctg gattacatat 
     1501 ctaataaagc cgtaaggaga cgggttcaaa aaggtttaaa taaaggagaa gcaatcaatg 
     1561 cattagctag aactatattt tttggacaac gtggagaatt tagagaacgt gctctccaag 
     1621 accagttaca aagagctagt gcactaaaca taattattaa cgctataagt gtgtggaaca 
     1681 ctgtatatat ggaaaaagcc gtagaagaat taaaagcaag aggagaattt agagaagatt 
     1741 taatgccata tgcgtggccg ttaggatggg aacatatcaa ttttcttgga gaatacaaat 
     1801 ttgaaggatt acatgacact gggcaaatga atttacgtcc tttacgtata aaagagccgt 
     1861 tttattctta atataacggc tctttttata gaaaaaatcc ttagcgtggt ttttttccga 
     1921 aatgctggcg gtaccccaag aattagaaat gagtagatca aattattcac gaatagaatc 
     1981 aggaaaatca gatccaacca taaaaacact agaacaaatt gcaaagttaa ctaactcaac 
     2041 gctagtagtg gatttaatcc caaatgagcc aacagaacca gagccagaaa cagaatcaga 
     2101 acaagtaaca ttggatttag aaatggaaga agaaaaaagc aatgacttcg tgtgaataat 
     2161 gcacgaaatc gttgcttatt tttttttaaa agcggtatac tagatataac gaaacaacga 
     2221 actgaataga aacgaaaaaa gagccatgac acatttataa aatgtttgac gacattttat 
     2281 aaatgcatag cccgataaga ttgccaaacc aacgcttatc agttagtcag atgaactctt 
     2341 ccctcgtaag aagttattta attaactttg tttgaagacg gtatataacc gtactatcat 
     2401 tatataggga aatcagagag ttttcaagta tctaagctac tgaatttaag aattgttaag 
     2461 caatcaatcg gaaatcgttt gattgctttt tttgtattca tttatagaag gtggagtttg 
     2521 tatgaatcat gatgaatgta aaacttatat aaaaaatagt ttattggaga taagaaaatt 
     2581 agcaaatatc tatacactag aaacgtttaa gaaagagtta gaaaagagaa atatctactt 
     2641 agaaacaaaa tcagataagt atttttcttc ggagggggaa gattatatat ataagttaat 
     2701 agaaaataac aaaataattt attcgattag tggaaaaaaa ttgacttata aaggaaaaaa 
     2761 atctttttca aaacatgcaa tattgaaaca gttgaatgaa aaagcaaacc aagttaatta 
     2821 aacaacctat tttataggat ttataggaaa ggagaacagc tgaatgaata tcccttttgt 
     2881 tgtagaaact gtgcttcatg acggcttgtt aaagtacaaa tttaaaaata gtaaaattcg 
     2941 ctcaatcact accaagccag gtaaaagcaa aggggctatt tttgcgtatc gctcaaaatc 
     3001 aagcatgatt ggcggtcgtg gtgttgttct gacttccgag gaagcgattc aagaaaatca 
     3061 agatacattt acacattgga cacccaacgt ttatcgttat ggaacgtatg cagacgaaaa 
     3121 ccgttcatac acgaaaggac attctgaaaa caatttaaga caaatcaata ccttctttat 
     3181 tgattttgat attcacacgg caaaagaaac tatttcagca agcgatattt taacaaccgc 
     3241 tattgattta ggttttatgc ctactatgat tatcaaatct gataaaggtt atcaagcata 
     3301 ttttgtttta gaaacgccag tctatgtgac ttcaaaatca gaatttaaat ctgtcaaagc 
     3361 agccaaaata atttcgcaaa atatccgaga atattttgga aagtctttgc cagttgatct 
     3421 aacgtgtaat cattttggta ttgctcgcat accaagaacg gacaatgtag aattttttga 
     3481 tcctaattac cgttattctt tcaaagaatg gcaagattgg tctttcaaac aaacagataa 
     3541 taagggcttt actcgttcaa gtctaacggt tttaagcggt acagaaggca aaaaacaagt 
     3601 agatgaaccc tggtttaatc tcttattgca cgaaacgaaa ttttcaggag aaaagggttt 
     3661 aatagggcgt aataacgtca tgtttaccct ctctttagcc tactttagtt caggctattc 
     3721 aatcgaaacg tgcgaatata atatgtttga gtttaataat cgattagatc aacccttaga 
     3781 agaaaaagaa gtaatcaaaa ttgttagaag tgcctattca gaaaactatc aaggggctaa 
     3841 tagggaatac attaccattc tttgcaaagc ttgggtatca agtgatttaa ccagtaaaga 
     3901 tttatttgtc cgtcaagggt ggtttaaatt caagaaaaaa agaagcgaac gtcaacgtgt 
     3961 tcatttgtca gaatggaaag aagatttaat ggcttatatt agcgaaaaaa gcgatgtata 
     4021 caagccttat ttagtgacga ccaaaaaaga gattagagaa gtgctaggca ttcctgaacg 
     4081 gacattagat aaattgctga aggtactgaa ggcgaatcag gaaattttct ttaagattaa 
     4141 accaggaaga aatggtggca ttcaacttgc tagtgttaaa tcattgttgc tatcgatcat 
     4201 taaagtaaaa aaagaagaaa aagaaagcta tataaaggcg ctgacaaatt cttttgactt 
     4261 agagcataca ttcattcaag agactttaaa caagctagca gaacgcccta aaacggacac 
     4321 acaactcgat ttgtttagct atgatacagg ctgaaaataa aacccgcact atgccattac 
     4381 atttatatct atgatacgtg tttgtttttt ctttgctgtt tagcgaatga ttagcagaaa 
     4441 tatacagagt aagattttaa ttaattatta gggggagaag gagagagtag cccgaaaact 
     4501 tttagttggc ttggactgaa cgaagtgagg gaaaggctac taaaacgtcg aggggcagtg 
     4561 agagcgaagc gaacacttga ttttttaatt ttctatcttt tataggtcat tagagtatac 
     4621 ttatttgtcc tataaactat ttagcagcat aatagattta ttgaataggt catttaagtt 
     4681 gagcatatta gaggaggaaa atcttggaga aatatttgaa gaacccgatt acatggattg 
     4741 gattagttct tgtggttacg tggtttttaa ctaaaagtag tgaatttttg atttttggtg 
     4801 tgtgtgtctt gttgttagta tttgctagtc aaagtgatta aatagaattc cggatgagca 
     4861 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt 
     4921 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca 
     4981 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta 
     5041 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca 
     5101 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tcttacgtgc 
     5161 cgatcaacgt ctcattttcg ccaaaagttg gcccagggct tcccggtatc aacagggaca 
     5221 ccaggattta tttattctgc gaagtgatct tccgtcacag gtatttattc ggcgcaaagt 
     5281 gcgtcgggtg atgctgccaa cttactgatt tagtgtatga tggtgttttt gaggtgctcc 
     5341 agtggcttct gtttctatca gctgtccctc ctgttcagct actgacgggg tggtgcgtaa 
     5401 cggcaaaagc accgccggac atcagcgcta gcggagtgta tactggctta ctatgttggc 
     5461 actgatgagg gtgtcagtga agtgcttcat gtggcaggag aaaaaaggct gcaccggtgc 
     5521 gtcagcagaa tatgtgatac aggatatatt ccgcttcctc gctcactgac tcgctacgct 
     5581 cggtcgttcg actgcggcga gcggaaatgg cttacgaacg gggcggagat ttcctggaag 
     5641 atgccaggaa gatacttaac agggaagtga gagggccgcg gcaaagccgt ttttccatag 
     5701 gctccgcccc cctgacaagc atcacgaaat ctgacgctca aatcagtggt ggcgaaaccc 
     5761 gacaggacta taaagatacc aggcgtttcc ccctggcggc tccctcgtgc gctctcctgt 
     5821 tcctgccttt cggtttaccg gtgtcattcc gctgttatgg ccgcgtttgt ctcattccac 
     5881 gcctgacact cagttccggg taggcagttc gctccaagct ggactgtatg cacgaacccc 
     5941 ccgttcagtc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggaaa 
     6001 gacatgcaaa agcaccactg gcagcagcca ctggtaattg atttagagga gttagtcttg 
     6061 aagtcatgcg ccggttaagg ctaaactgaa aggacaagtt ttggtgactg cgctcctcca 
     6121 agccagttac ctcggttcaa agagttggta gctcagagaa ccttcgaaaa accgccctgc 
     6181 aaggcggttt tttcgttttc agagcaagag attacgcgca gaccaaaacg atctcaagaa 
     6241 gatcatctta ttaatcagat aaaatatttc tagatttcag tgcaatttat ctcttcaaat 
     6301 gtagcacctg aagtcagccc catacgatat aagttgtaat tctcatgttt gacagcttat 
     6361 catcgataag ctttaatgcg gtagtttatc acagttaaat tgctaacgca gtcaggcacc 
     6421 gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc tggatgctgt 
     6481 aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg tccattccga 
     6541 cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc aatttctatg 
     6601 cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc tgctcgcttc 
     6661 gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc tgtggatcct 
     6721 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta 
     6781 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg 
     6841 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt 
     6901 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt 
     6961 cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag ccttcaaccc 
     7021 agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta tgactgtctt 
     7081 ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt tcggcgagga 
     7141 ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg gaatcttgca 
     7201 cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg agaagcaggc 
     7261 cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt tcgcgacgcg 
     7321 aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg ggatgcccgc 
     7381 gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc ttcaaggatc 
     7441 gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca cggcgattta 
     7501 tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg ccctatacct 
     7561 tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga cctgaatgga 
     7621 agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa tcaattcttg 
     7681 cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc gtccgccatc 
     7741 tccagcagcc gcacgcggcg catctc 
//
