LOCUS       pGBF-NLScre             5024 bp    DNA     circular     22-OCT-2015
DEFINITION  LMBP 4475.
SOURCE      
  ORGANISM  
COMMENT     Accession number: 4475
            
            
COMMENT     VNTNAME|pGBF-NLScre|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pGBF-NLScre" 0 5024 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "BglI" "HaeII" "NheI" "SpeI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 5024 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 680 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BglI" "gccnnnnnggc" 8 0 1 3894 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "HaeII" "rgcgcy" 6 0 5 6 0 1148 0 2110 0 2358 0 
COMMENT            4660 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 296 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "SpeI" "actagt" 2 0 2 263 0 1357 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 2 533 0 3412 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 0 95 178 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "95..178") 
COMMENT        #7=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 179 216 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "179..216") 
COMMENT        #8=(CFSignal (CObList) "T3 promoter" 30 0 0 242 258 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "242..258") 
COMMENT        #9=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 2226 3020 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2226..3020") 
COMMENT        #10=(CFSignal (CObList) "ampicillin resistance" 4 0 0 3224 4084 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3224..4084") 
COMMENT        #11=(CFSignal (CObList) "T7 promoter" 30 0 1 1398 1414 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(1398..1414)") 
COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 0 4625 3 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4625..3") 
COMMENT        #13=(CFSignal (CObList) "cre" 4 0 0 327 1355 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "327..1355") 
COMMENT        #14=(CFSignal (CObList) "SV40 large T NLS" 4 0 0 309 326 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "LMBP 4475" 2 0 0 0 0 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pGBF-NLScre'" 1) 
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COMMENT                          1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
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COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5024 bp" 1) 
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COMMENT        #232=(CSampFrag "pGBF-NLScre" 2302 2358 "HaeII" 4660 "HaeII" 
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COMMENT        #233=(CSampFrag "pGBF-NLScre" 1142 6 "HaeII" 1148 "HaeII" (LOGPEN 0 0 0) 
COMMENT              1.37953e-316 2.07952e-294) 
COMMENT        #234=(CSampFrag "pGBF-NLScre" 962 1148 "HaeII" 2110 "HaeII" 
COMMENT              (LOGPEN 0 0 0) 1.73257e+098 6.98395e-315) 
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FEATURES             Location/Qualifiers
     promoter        95..178
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             179..216
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        242..258
                     /vntifkey="30"
                     /label=T3\promoter
     CDS             2226..3020
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     CDS             3224..4084
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        complement(1398..1414)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      4625..3
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             327..1355
                     /vntifkey="4"
                     /label=cre
     CDS             309..326
                     /vntifkey="4"
                     /label=SV40\large\T\NLS
BASE COUNT     1223 a      1242 c      1351 g      1208 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca 
      241 gaattaaccc tcactaaagg gactagtcct gcaggtttaa acgaattcgc ccttgctagc 
      301 ccaccatgcc caagaagaag aaggtgtcca atttactgac cgtacaccaa aatttgcctg 
      361 cattaccggt cgatgcaacg agtgatgagg ttcgcaagaa cctgatggac atgttcaggg 
      421 atcgccaggc gttttctgag catacctgga aaatgcttct gtccgtttgc cggtcgtggg 
      481 cggcatggtg caagttgaat aaccggaaat ggtttcccgc agaacctgaa gatgttcgcg 
      541 attatcttct atatcttcag gcgcgcggtc tggcagtaaa aactatccag caacatttgg 
      601 gccagctaaa catgcttcat cgtcggtccg ggctgccacg accaagtgac agcaatgctg 
      661 tttcactggt tatgcggcgg atccgaaaag aaaacgttga tgccggtgaa cgtgcaaaac 
      721 aggctctagc gttcgaacgc actgatttcg accaggttcg ttcactcatg gaaaatagcg 
      781 atcgctgcca ggatatacgt aatctggcat ttctggggat tgcttataac accctgttac 
      841 gtatagccga aattgccagg atcagggtta aagatatctc acgtactgac ggtgggagaa 
      901 tgttaatcca tattggcaga acgaaaacgc tggttagcac cgcaggtgta gagaaggcac 
      961 ttagcctggg ggtaactaaa ctggtcgagc gatggatttc cgtctctggt gtagctgatg 
     1021 atccgaataa ctacctgttt tgccgggtca gaaaaaatgg tgttgccgcg ccatctgcca 
     1081 ccagccagct atcaactcgc gccctggaag ggatttttga agcaactcat cgattgattt 
     1141 acggcgctaa ggatgactct ggtcagagat acctggcctg gtctggacac agtgcccgtg 
     1201 tcggagccgc gcgagatatg gcccgcgctg gagtttcaat accggagatc atgcaagctg 
     1261 gtggctggac caatgtaaat attgtcatga actatatccg taacctggat agtgaaacag 
     1321 gggcaatggt gcgcctgctg gaagatggcg attagactag taagggcgaa ttcgcggccg 
     1381 ctaaattcaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac 
     1441 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
     1501 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca 
     1561 gcctatacgt acggcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt 
     1621 ttgtggatgt acagagtgat attattgaca cgccggggcg acggatggtg atccccctgg 
     1681 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg 
     1741 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg 
     1801 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga 
     1861 tgttctgggg aatataaatg tcaggcatga gattatcaaa aaggatcttc acctagatcc 
     1921 ttttcacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat gtcagctact 
     1981 gggctatctg gacaagggaa aacgcaagcg caaagagaaa gcaggtagct tgcagtgggc 
     2041 ttacatggcg atagctagac tgggcggttt tatggacagc aagcgaaccg gaattgccag 
     2101 ctggggcgcc ctctggtaag gttgggaagc cctgcaaagt aaactggatg gctttctcgc 
     2161 cgccaaggat ctgatggcgc aggggatcaa gctctgatca agagacagga tgaggatcgt 
     2221 ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg gtggagaggc 
     2281 tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc gtgttccggc 
     2341 tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt gccctgaatg 
     2401 aactgcaaga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt ccttgcgcag 
     2461 ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc gaagtgccgg 
     2521 ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc atggctgatg 
     2581 caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac caagcgaaac 
     2641 atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag gatgatctgg 
     2701 acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag gcgagcatgc 
     2761 ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat atcatggtgg 
     2821 aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg gaccgctatc 
     2881 aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa tgggctgacc 
     2941 gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc ttctatcgcc 
     3001 ttcttgacga gttcttctga attattaacg cttacaattt cctgatgcgg tattttctcc 
     3061 ttacgcatct gtgcggtatt tcacaccgca tacaggtggc acttttcggg gaaatgtgcg 
     3121 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
     3181 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
     3241 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
     3301 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
     3361 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 
     3421 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 
     3481 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
     3541 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
     3601 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
     3661 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
     3721 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
     3781 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
     3841 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
     3901 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
     3961 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
     4021 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
     4081 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 
     4141 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     4201 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     4261 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     4321 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     4381 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 
     4441 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     4501 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     4561 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     4621 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     4681 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     4741 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     4801 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     4861 ctttttacgg ttcctgggct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     4921 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 
     4981 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag 
//
