LOCUS       pEFlacZneoflox          9224 bp    DNA     circular     22-OCT-2015
DEFINITION  LMBP 4478.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|635275689|
COMMENT     VNTNAME|pEFlacZneoflox|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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FEATURES             Location/Qualifiers
     rep_origin      8825..3
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        95..178
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             179..216
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     rep_origin      6582..7037
                     /vntifkey="33"
                     /label=f1\ori
     CDS             7424..8284
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        240..411
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      368..443
                     /vntifkey="33"
                     /label=SV40\ori
     CDS             482..551
                     /vntifkey="4"
                     /label=part\of\SV40\small\t-antigen
     promoter        592..1735
                     /vntifkey="29"
                     /label=hEF1\promoter
     intron          788..1726
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     CDS             2674..5721
                     /vntifkey="4"
                     /label=lacZ\fragment
     terminator      5740..6306
                     /vntifkey="43"
                     /label=3'\UTR\of\hGM-CSF,\incl.\polyA
     misc_feature    1799..1832
                     /vntifkey="21"
                     /label=loxP
     misc_feature    2634..2667
                     /vntifkey="21"
                     /label=loxP
     CDS             1833..2633
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     CDS             1772..1798
                     /vntifkey="4"
                     /label=lacZ\fragment
BASE COUNT     2029 a      2452 c      2548 g      2183 t        12 others
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctta 
      241 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc 
      301 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc 
      361 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta 
      421 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttgcaaa 
      481 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga 
      541 aaggagtgcc tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 
      601 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg 
      661 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 
      721 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 
      781 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc 
      841 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg 
      901 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc 
      961 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg 
     1021 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc 
     1081 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat 
     1141 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc 
     1201 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg 
     1261 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct 
     1321 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg 
     1381 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag 
     1441 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc 
     1501 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga 
     1561 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc 
     1621 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat 
     1681 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat 
     1741 tctctaggca tgcctgcagg tcgactctag aatgaccatg attacggatt cactggccat 
     1801 aacttcgtat agcatacatt atacgaagtt atatgattga acaagatgga ttgcacgcag 
     1861 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 
     1921 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 
     1981 agaccgacct gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc 
     2041 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 
     2101 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 
     2161 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 
     2221 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 
     2281 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 
     2341 tgttcgccag gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg 
     2401 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 
     2461 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 
     2521 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 
     2581 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgnnnn nnaataactt 
     2641 cgtatagcat acattatacg aagttatnnn nnngtcgttt tacaacgtcg tgactgggaa 
     2701 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt 
     2761 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa 
     2821 tggcgctttg cctggtttcc ggcaccagaa gcggtaccgg aaagctggct ggagtgcgat 
     2881 cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc agatgcacgg ttacgatgcg 
     2941 cccatctaca ccaacgtaac ctatcccatt acggtcaatc cgccgtttgt tcccacggag 
     3001 aatccgacgg gttgttactc gctcacattt aatgttgatg aaagctggct acaggaaggc 
     3061 cagacgcgaa ttatttttga tggcgttaac tcggcgtttc atctgtggtg caacgggcgc 
     3121 tgggtcggtt acggccagga cagtcgtttg ccgtctgaat ttgacctgag cgcattttta 
     3181 cgcgccggag aaaaccgcct cgcggtgatg gtgctgcgtt ggagtgacgg cagttatctg 
     3241 gaagatcagg atatgtggcg gatgagcggc attttccgtg acgtctcgtt gctgcataaa 
     3301 ccgactacac aaatcagcga tttccatgtt gccactcgct ttaatgatga tttcagccgc 
     3361 gctgtactgg aggctgaagt tcagatgtgc ggcgagttgc gtgactacct acgggtaaca 
     3421 gtttctttat ggcagggtga aacgcaggtc gccagcggca ccgcgccttt cggcggtgaa 
     3481 attatcgatg agcgtggtgg ttatgccgat cgcgtcacac tacgtctgaa cgtcgaaaac 
     3541 ccgaaactgt ggagcgccga aatcccgaat ctctatcgtg cggtggttga actgcacacc 
     3601 gccgacggca cgctgattga agcagaagcc tgcgatgtcg gtttccgcga ggtgcggatt 
     3661 gaaaatggtc tgctgctgct gaacggcaag ccgttgctga ttcgaggcgt taaccgtcac 
     3721 gagcatcatc ctctgcatgg tcaggtcatg gatgagcaga cgatggtgca ggatatcctg 
     3781 ctgatgaagc agaacaactt taacgccgtg cgctgttcgc attatccgaa ccatccgctg 
     3841 tggtacacgc tgtgcgaccg ctacggcctg tatgtggtgg atgaagccaa tattgaaacc 
     3901 cacggcatgg tgccaatgaa tcgtctgacc gatgatccgc gctggctacc ggcgatgagc 
     3961 gaacgcgtaa cgcgaatggt gcagcgcgat cgtaatcacc cgagtgtgat catctggtcg 
     4021 ctggggaatg aatcaggcca cggcgctaat cacgacgcgc tgtatcgctg gatcaaatct 
     4081 gtcgatcctt cccgcccggt gcagtatgaa ggcggcggag ccgacaccac ggccaccgat 
     4141 attatttgcc cgatgtacgc gcgcgtggat gaagaccagc ccttcccggc tgtgccgaaa 
     4201 tggtccatca aaaaatggct ttcgctacct ggagagacgc gcccgctgat cctttgcgaa 
     4261 tacgcccacg cgatgggtaa cagtcttggc ggtttcgcta aatactggca ggcgtttcgt 
     4321 cagtatcccc gtttacaggg cggcttcgtc tgggactggg tggatcagtc gctgattaaa 
     4381 tatgatgaaa acggcaaccc gtggtcggct tacggcggtg attttggcga tacgccgaac 
     4441 gatcgccagt tctgtatgaa cggtctggtc tttgccgacc gcacgccgca tccagcgctg 
     4501 acggaagcaa aacaccagca gcagtttttc cagttccgtt tatccgggca aaccatcgaa 
     4561 gtgaccagcg aatacctgtt ccgtcatagc gataacgagc tcctgcactg gatggtggcg 
     4621 ctggatggta agccgctggc aagcggtgaa gtgcctctgg atgtcgctcc acaaggtaaa 
     4681 cagttgattg aactgcctga actaccgcag ccggagagcg ccgggcaact ctggctcaca 
     4741 gtacgcgtag tgcaaccgaa cgcgaccgca tggtcagaag ccgggcacat cagcgcctgg 
     4801 cagcagtggc gtctggcgga aaacctcagt gtgacgctcc ccgccgcgtc ccacgccatc 
     4861 ccgcatctga ccaccagcga aatggatttt tgcatcgagc tgggtaataa gcgttggcaa 
     4921 tttaaccgcc agtcaggctt tctttcacag atgtggattg gcgataaaaa acaactgctg 
     4981 acgccgctgc gcgatcagtt cacccgtgca ccgctggata acgacattgg cgtaagtgaa 
     5041 gcgacccgca ttgaccctaa cgcctgggtc gaacgctgga aggcggcggg ccattaccag 
     5101 gccgaagcag cgttgttgca gtgcacggca gatacacttg ctgatgcggt gctgattacg 
     5161 accgctcacg cgtggcagca tcaggggaaa accttattta tcagccggaa aacctaccgg 
     5221 attgatggta gtggtcaaat ggcgattacc gttgatgttg aagtggcgag cgatacaccg 
     5281 catccggcgc ggattggcct gaactgccag ctggcgcagg tagcagagcg ggtaaactgg 
     5341 ctcggattag ggccgcaaga aaactatccc gaccgcctta ctgccgcctg ttttgaccgc 
     5401 tgggatctgc cattgtcaga catgtatacc ccgtacgtct tcccgagcga aaacggtctg 
     5461 cgctgcggga cgcgcgaatt gaattatggc ccacaccagt ggcgcggcga cttccagttc 
     5521 aacatcagcc gctacagtca acagcaactg atggaaacca gccatcgcca tctgctgcac 
     5581 gcggaagaag gcacatggct gaatatcgac ggtttccata tggggattgg tggcgacgac 
     5641 tcctggagcc cgtcagtatc ggcggaattc cagctgagcg ccggtcgcta ccattaccag 
     5701 ttggtctggt gtcaaaaata agcggccgcg actctagagt gagggtcccc acctgggacc 
     5761 cttgagagta tcaggtctcc cacgtgggag acaagaaatc cctgtttaat atttaaacag 
     5821 cagtgttccc catctgggtc cttgcacccc tcactctggc ctcagccgac tgcacagcgg 
     5881 cccctgcatc cccttggctg tgaggcccct ggacaagcag aggtggccag agctgggagg 
     5941 catggccctg gggtcccacg aatttgctgg ggaatctcgt ttttcttctt aagacttttg 
     6001 ggacatggtt tgactcccga acatcaccga cgtgtctcct gtttttctgg gtggcctcgg 
     6061 gacacctgcc ctgcccccac gagggtcagg actgtgactc tttttagggc caggcaggtg 
     6121 cctggacatt tgccttgctg gatggggact ggggatgtgg gagggagcag acaggaggaa 
     6181 tcatgtcagg cctgtgtgtg aaaggaagct ccactgtcac cctccacctc ttcacccccc 
     6241 actcaccagt gtcccctcca ctgtcacatt gtaactgaac ttcaggataa taaagtgttt 
     6301 gcctccaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaagaat tcactggccg 
     6361 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag 
     6421 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc 
     6481 aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc 
     6541 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc 
     6601 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 
     6661 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 
     6721 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 
     6781 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt 
     6841 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 
     6901 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc 
     6961 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 
     7021 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt 
     7081 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc 
     7141 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc 
     7201 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt 
     7261 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 
     7321 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 
     7381 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 
     7441 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 
     7501 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 
     7561 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 
     7621 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca 
     7681 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 
     7741 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 
     7801 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 
     7861 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 
     7921 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac 
     7981 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 
     8041 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 
     8101 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 
     8161 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 
     8221 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 
     8281 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 
     8341 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 
     8401 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 
     8461 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 
     8521 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 
     8581 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 
     8641 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 
     8701 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 
     8761 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 
     8821 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 
     8881 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 
     8941 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 
     9001 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 
     9061 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 
     9121 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 
     9181 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag 
//
