LOCUS       pEFlacZ                 8349 bp    DNA     circular     22-OCT-2015
DEFINITION  LMBP 4479.
SOURCE      
  ORGANISM  
COMMENT     VNTDATE|635274720|
COMMENT     VNTNAME|pEFlacZ|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEFlacZ" 0 8349 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
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COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "EcoRV" "HindIII" "KpnI" "NotI" "XbaI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
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COMMENT       (CMolPar 0 0 1 1767 4858 1 8349 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 2900 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 2 235 0 470 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1983 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 4849 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XbaI" "tctaga" 2 0 2 1767 0 4859 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList) 1 1 1 1 "7950..3") 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 0 95 178 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "95..178") 
COMMENT        #7=(CFSignal (CObList) "5' UTR of lacZ, incl. RBS" 32 0 0 179 216 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "179..216") 
COMMENT        #8=(CFSignal (CObList) "f1 ori" 33 0 0 5707 6162 0 (CStringList) 
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COMMENT        #9=(CFSignal (CObList) "ampicillin resistance" 4 0 0 6549 7409 0 
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COMMENT        #10=(CFSignal (CObList) "part of SV40 small t-antigen" 4 0 0 482 551 0 
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COMMENT        #12=(CFSignal (CObList) "intron hEF1 promoter" 15 0 0 788 1726 0 
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COMMENT        #13=(CFSignal (CObList) "lacZ" 4 0 0 1772 4846 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pEFlacZ'" 1) 
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COMMENT                          "Created: 08/24/12 05:12" 1) 
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COMMENT                          "Last Modified: 08/24/12 05:12" 1) 
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COMMENT                          "length: 8349 bp" 1) 
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FEATURES             Location/Qualifiers
     rep_origin      7950..3
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        95..178
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             179..216
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     rep_origin      5707..6162
                     /vntifkey="33"
                     /label=f1\ori
     CDS             6549..7409
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             482..551
                     /vntifkey="4"
                     /label=part\of\SV40\small\t-antigen
     promoter        592..1735
                     /vntifkey="29"
                     /label=hEF1\promoter
     intron          788..1726
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     CDS             1772..4846
                     /vntifkey="4"
                     /label=lacZ
     terminator      4865..5431
                     /vntifkey="43"
                     /label=3'\UTR\of\hGM-CSF,\incl.\polyA
BASE COUNT     1810 a      2142 c      2241 g      1927 t       229 others
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttn 
      241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn 
      421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnaa gctttgcaaa 
      481 gatggataaa gttttaaaca gagaggaatc tttgcagcta atggaccttc taggtcttga 
      541 aaggagtgcc tcgtgaggct ccggtgcccg tcagtgggca gagcgcacat cgcccacagt 
      601 ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg 
      661 ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg gtgggggaga 
      721 accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag 
      781 aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg gttatggccc 
      841 ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc ccgagcttcg 
      901 ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc 
      961 ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg 
     1021 cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat gacctgctgc 
     1081 gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc acactggtat 
     1141 ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc 
     1201 gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc aagctggccg 
     1261 gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct 
     1321 ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc ctgctgcagg 
     1381 gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag 
     1441 gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt accgggcgcc 
     1501 gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag gttgggggga 
     1561 ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag ttaggccagc 
     1621 ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat cttggttcat 
     1681 tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat 
     1741 tctctaggca tgcctgcagg tcgactctag aatgaccatg attacggatt cactggccgt 
     1801 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc 
     1861 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca 
     1921 acagttgcgc agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt 
     1981 accggaaagc tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa 
     2041 ctggcagatg cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt 
     2101 caatccgccg tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt 
     2161 tgatgaaagc tggctacagg aaggccagac gcgaattatt tttgatggcg ttaactcggc 
     2221 gtttcatctg tggtgcaacg ggcgctgggt cggttacggc caggacagtc gtttgccgtc 
     2281 tgaatttgac ctgagcgcat ttttacgcgc cggagaaaac cgcctcgcgg tgatggtgct 
     2341 gcgttggagt gacggcagtt atctggaaga tcaggatatg tggcggatga gcggcatttt 
     2401 ccgtgacgtc tcgttgctgc ataaaccgac tacacaaatc agcgatttcc atgttgccac 
     2461 tcgctttaat gatgatttca gccgcgctgt actggaggct gaagttcaga tgtgcggcga 
     2521 gttgcgtgac tacctacggg taacagtttc tttatggcag ggtgaaacgc aggtcgccag 
     2581 cggcaccgcg cctttcggcg gtgaaattat cgatgagcgt ggtggttatg ccgatcgcgt 
     2641 cacactacgt ctgaacgtcg aaaacccgaa actgtggagc gccgaaatcc cgaatctcta 
     2701 tcgtgcggtg gttgaactgc acaccgccga cggcacgctg attgaagcag aagcctgcga 
     2761 tgtcggtttc cgcgaggtgc ggattgaaaa tggtctgctg ctgctgaacg gcaagccgtt 
     2821 gctgattcga ggcgttaacc gtcacgagca tcatcctctg catggtcagg tcatggatga 
     2881 gcagacgatg gtgcaggata tcctgctgat gaagcagaac aactttaacg ccgtgcgctg 
     2941 ttcgcattat ccgaaccatc cgctgtggta cacgctgtgc gaccgctacg gcctgtatgt 
     3001 ggtggatgaa gccaatattg aaacccacgg catggtgcca atgaatcgtc tgaccgatga 
     3061 tccgcgctgg ctaccggcga tgagcgaacg cgtaacgcga atggtgcagc gcgatcgtaa 
     3121 tcacccgagt gtgatcatct ggtcgctggg gaatgaatca ggccacggcg ctaatcacga 
     3181 cgcgctgtat cgctggatca aatctgtcga tccttcccgc ccggtgcagt atgaaggcgg 
     3241 cggagccgac accacggcca ccgatattat ttgcccgatg tacgcgcgcg tggatgaaga 
     3301 ccagcccttc ccggctgtgc cgaaatggtc catcaaaaaa tggctttcgc tacctggaga 
     3361 gacgcgcccg ctgatccttt gcgaatacgc ccacgcgatg ggtaacagtc ttggcggttt 
     3421 cgctaaatac tggcaggcgt ttcgtcagta tccccgttta cagggcggct tcgtctggga 
     3481 ctgggtggat cagtcgctga ttaaatatga tgaaaacggc aacccgtggt cggcttacgg 
     3541 cggtgatttt ggcgatacgc cgaacgatcg ccagttctgt atgaacggtc tggtctttgc 
     3601 cgaccgcacg ccgcatccag cgctgacgga agcaaaacac cagcagcagt ttttccagtt 
     3661 ccgtttatcc gggcaaacca tcgaagtgac cagcgaatac ctgttccgtc atagcgataa 
     3721 cgagctcctg cactggatgg tggcgctgga tggtaagccg ctggcaagcg gtgaagtgcc 
     3781 tctggatgtc gctccacaag gtaaacagtt gattgaactg cctgaactac cgcagccgga 
     3841 gagcgccggg caactctggc tcacagtacg cgtagtgcaa ccgaacgcga ccgcatggtc 
     3901 agaagccggg cacatcagcg cctggcagca gtggcgtctg gcggaaaacc tcagtgtgac 
     3961 gctccccgcc gcgtcccacg ccatcccgca tctgaccacc agcgaaatgg atttttgcat 
     4021 cgagctgggt aataagcgtt ggcaatttaa ccgccagtca ggctttcttt cacagatgtg 
     4081 gattggcgat aaaaaacaac tgctgacgcc gctgcgcgat cagttcaccc gtgcaccgct 
     4141 ggataacgac attggcgtaa gtgaagcgac ccgcattgac cctaacgcct gggtcgaacg 
     4201 ctggaaggcg gcgggccatt accaggccga agcagcgttg ttgcagtgca cggcagatac 
     4261 acttgctgat gcggtgctga ttacgaccgc tcacgcgtgg cagcatcagg ggaaaacctt 
     4321 atttatcagc cggaaaacct accggattga tggtagtggt caaatggcga ttaccgttga 
     4381 tgttgaagtg gcgagcgata caccgcatcc ggcgcggatt ggcctgaact gccagctggc 
     4441 gcaggtagca gagcgggtaa actggctcgg attagggccg caagaaaact atcccgaccg 
     4501 ccttactgcc gcctgttttg accgctggga tctgccattg tcagacatgt ataccccgta 
     4561 cgtcttcccg agcgaaaacg gtctgcgctg cgggacgcgc gaattgaatt atggcccaca 
     4621 ccagtggcgc ggcgacttcc agttcaacat cagccgctac agtcaacagc aactgatgga 
     4681 aaccagccat cgccatctgc tgcacgcgga agaaggcaca tggctgaata tcgacggttt 
     4741 ccatatgggg attggtggcg acgactcctg gagcccgtca gtatcggcgg aattccagct 
     4801 gagcgccggt cgctaccatt accagttggt ctggtgtcaa aaataagcgg ccgcgactct 
     4861 agagtgaggg tccccacctg ggacccttga gagtatcagg tctcccacgt gggagacaag 
     4921 aaatccctgt ttaatattta aacagcagtg ttccccatct gggtccttgc acccctcact 
     4981 ctggcctcag ccgactgcac agcggcccct gcatcccctt ggctgtgagg cccctggaca 
     5041 agcagaggtg gccagagctg ggaggcatgg ccctggggtc ccacgaattt gctggggaat 
     5101 ctcgtttttc ttcttaagac ttttgggaca tggtttgact cccgaacatc accgacgtgt 
     5161 ctcctgtttt tctgggtggc ctcgggacac ctgccctgcc cccacgaggg tcaggactgt 
     5221 gactcttttt agggccaggc aggtgcctgg acatttgcct tgctggatgg ggactgggga 
     5281 tgtgggaggg agcagacagg aggaatcatg tcaggcctgt gtgtgaaagg aagctccact 
     5341 gtcaccctcc acctcttcac cccccactca ccagtgtccc ctccactgtc acattgtaac 
     5401 tgaacttcag gataataaag tgtttgcctc caaaaaaaaa aaaaaaaaaa aaaaaaaaaa 
     5461 aaaaaaaaaa agaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 
     5521 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 
     5581 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atggcgcctg 
     5641 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac 
     5701 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg 
     5761 tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc 
     5821 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc 
     5881 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta 
     5941 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta 
     6001 atagtggact cttgttccaa actggaacaa cactcaaccc tatctcgggc tattcttttg 
     6061 atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa 
     6121 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta 
     6181 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg 
     6241 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg 
     6301 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc 
     6361 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag 
     6421 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt 
     6481 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa 
     6541 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt 
     6601 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt 
     6661 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt 
     6721 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg 
     6781 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga 
     6841 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa 
     6901 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga 
     6961 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa 
     7021 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca 
     7081 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta 
     7141 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac 
     7201 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc 
     7261 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag 
     7321 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga 
     7381 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt 
     7441 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata 
     7501 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag 
     7561 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa 
     7621 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt 
     7681 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc 
     7741 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa 
     7801 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa 
     7861 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc 
     7921 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa 
     7981 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa 
     8041 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg 
     8101 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc 
     8161 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg 
     8221 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg 
     8281 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg 
     8341 aagcggaag 
//
