LOCUS       p1168hIL6mCRE-C/EBP-luc+        5986 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 4497.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4497
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 23-OCT-2007
            
COMMENT     VNTNAME|p1168hIL6mCRE-C/EBP-luc+|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "p1168hIL6mCRE-C/EBP-luc+" 0 5986 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "EcoRV" "PvuI" "HindIII" "XmaI" "KpnI" "NotI" 
COMMENT         "SalI") (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList) 1 "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 
COMMENT        0 (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 2213 2212 1 5986 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 3 32 0 1046 0 3173 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "EcoRV" "gatatc" 4 0 1 1057 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 4692 0 5738 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1222 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 27 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 6 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 5820 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "SalI" "gtcgac" 2 0 1 3179 0 0 "")) 
COMMENT       (CObList 
COMMENT        #8=(CFSignal (CObList) "5' FR of hIL6" 21 0 0 32 672 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "32..672") 
COMMENT        #9=(CFSignal (CObList) "hIL6 promoter mutant" 29 0 0 673 1208 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "673..1208") 
COMMENT        #10=(CFSignal (CObList) "LUCm" 4 0 0 1256 2908 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1256..2908") 
COMMENT        #11=(CFSignal (CObList) "SV40 polyA late" 43 0 0 2996 3092 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2996..3092") 
COMMENT        #12=(CFSignal (CObList) "SV40 polyA early" 43 0 1 2996 3092 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2996..3092)") 
COMMENT        #13=(CFSignal (CObList) "pMB1 ori" 33 0 1 3305 3707 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3305..3707)") 
COMMENT        #14=(CFSignal (CObList) "ampicillin" 4 0 1 4248 5108 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4248..5108)") 
COMMENT        #15=(CFSignal (CObList) "f1 ori" 33 0 0 5240 5695 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5240..5695") 
COMMENT        #16=(CFSignal (CObList) "synthetic polyA" 43 0 1 5826 5979 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5826..5979)")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #17=(CFSignal (CObList) "LMBP 4497" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #18=(CFSignal (CObList) "Last modified: 23-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #19=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #20=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) 
COMMENT                    "p1168hIL6mCRE-C/EBP-luc+" 2) 5 "" 0 4) 
COMMENT              #21=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'p1168hIL6mCRE-C/EBP-luc+'" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4497." 
COMMENT                          1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/23/07 04:15" 1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5986 bp" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #30=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #32=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 1 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #34=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #35=(CLinePar (CParagraph 0 (15 #17# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 4497") 
COMMENT                     #36=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #37=(CLinePar (CParagraph 0 (15 #18# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 23-OCT-2007"))) 
COMMENT                   "Annotations") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #40=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1256 (3 #10# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #41=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1256  End: 2908" 1) 
COMMENT                                   #42=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mutated firefly luciferase coding sequence" 
COMMENT                                        1) 
COMMENT                                   #43=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #44=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1256..2908" 1))) "LUCm") 
COMMENT                            #45=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4248 (3 #14# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #46=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4248  End: 5108 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "antibiotic resistance gene; coding sequence" 
COMMENT                                        1) 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4248..5108)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (2 total)") 
COMMENT                     #50=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #51=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 32 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 32    End: 672 " 1) 
COMMENT                                   #53=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "5' flanking region of the human interleukin 6 gene" 
COMMENT                                        1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #55=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   32..672" 1))) "5' FR of hIL6"))) 
COMMENT                          "Misc. Feature (1 total)") 
COMMENT                     #56=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #57=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 673 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 673   End: 1208" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mutated human interleukin 6 promoter" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   673..1208" 1))) 
COMMENT                                 "hIL6 promoter mutant"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #62=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #63=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3305 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3305  End: 3707 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(3305..3707)" 1))) 
COMMENT                                 "pMB1 ori") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5240 (3 #15# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5240  End: 5695" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "phage f1 origin" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   5240..5695" 1))) "f1 ori"))) 
COMMENT                          "Replication Origin (2 total)") 
COMMENT                     #73=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2996 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2996  End: 3092" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "polyA signal of the SV40 late region" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2996..3092" 1))) "SV40 polyA late") 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2996 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2996  End: 3092 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "polyA signal of the SV40 early region" 
COMMENT                                        1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #83=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(2996..3092)" 1))) 
COMMENT                                 "SV40 polyA early") 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5826 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5826  End: 5979 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "synthetic polyA signal" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #88=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5826..5979)" 1))) 
COMMENT                                 "synthetic polyA"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #89=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #90=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 14391120 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #91=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 32 " 1) 
COMMENT                                   #93=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 2) 1 2 2 0 191) 
COMMENT                                        " N2: 1046 " 1) 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #0# 3) 1 2 2 0 191) 
COMMENT                                        " N3: 3173 " 1))))) "BamHI: 3 sites") 1 
COMMENT                          5 "GGATCC" "CCTAGG") 
COMMENT                     #95=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 27385232 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #96=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #97=(CLinePar 
COMMENT                                        (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                        " N1: 1057 " 1))))) "EcoRV: 1 site") 3 3 
COMMENT                          "GATATC" "CTATAG") 
COMMENT                     #98=(CRSFolderPar 
COMMENT                          (CFolderPar 
COMMENT                           (CGroupPar (CParagraph 21802664 (8 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #99=(CGroupPar 
COMMENT                                  (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                  (CObjectList 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                         " N1: 1222 " 1))))) "HindIII: 1 site") 
COMMENT                          1 5 "AAGCTT" "TTCGAA") 
COMMENT                     #101=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 21504032 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #102=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #103=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 6 " 1))))) "KpnI: 1 site") 5 1 
COMMENT                           "GGTACC" "CCATGG") 
COMMENT                     #104=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 21439144 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #105=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #6# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #6# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 5820 " 1))))) "NotI: 1 site") 2 
COMMENT                           6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                     #107=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 28081368 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #108=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 4692 " 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #2# 2) 1 2 2 0 191) 
COMMENT                                           " N2: 5738 " 1))))) "PvuI: 2 sites") 
COMMENT                           4 2 "CGATCG" "GCTAGC") 
COMMENT                     #111=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 21511608 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #112=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #7# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #7# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 3179 " 1))))) "SalI: 1 site") 1 
COMMENT                           5 "GTCGAC" "CAGCTG") 
COMMENT                     #114=(CRSFolderPar 
COMMENT                           (CFolderPar 
COMMENT                            (CGroupPar (CParagraph 22141728 (8 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #115=(CGroupPar 
COMMENT                                    (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                    (CObjectList 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                           " N1: 27 " 1))))) "XmaI: 1 site") 1 5 
COMMENT                           "CCCGGG" "GGGCCC"))) "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #117=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #118=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #119=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #120=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #121=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #122=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #123=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #124=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #125=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #126=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #127=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #128=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #129=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #130=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #131=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #132=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #133=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #134=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #135=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #136=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #137=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #138=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #139=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #140=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #141=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #142=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #143=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #144=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #145=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #146=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #147=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #148=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #149=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #150=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 49216) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 65535 "@N" 0) 
COMMENT          #151=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #152=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #153=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #154=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #155=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 16711680 
COMMENT                1 5 "@N (@S)" 0) 
COMMENT          #156=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #157=(CWidgetStyle "~Scrap" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #158=(CWidgetStyle "Label 0" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711935 1 5 "@N (@S)" 0) 
COMMENT          #159=(CWidgetStyle "Label 1" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #160=(CWidgetStyle "Label 5" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #161=(CWidgetStyle "Intron Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #162=(CWidgetStyle "Misc_Feature Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #163=(CWidgetStyle "Promoter_E Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #164=(CWidgetStyle "Promoter_P Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #165=(CWidgetStyle "Rep_Origin Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #166=(CWidgetStyle "Terminator Label" 1 (LOGPEN 0 0 3342336) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #167=(CWidgetStyle "oriT Label" 1 (LOGPEN 0 0 13107) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #168=(CWidgetStyle "Label 6" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #169=(CWidgetStyle "Intron" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #170=(CWidgetStyle "Label 7" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #171=(CWidgetStyle "Label 8" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #172=(CWidgetStyle "Label 9" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #173=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #174=(CWidgetStyle "Label 11" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #175=(CWidgetStyle "Label 12" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #176=(CWidgetStyle "Uniq RSite" 1 (LOGPEN 0 0 16750899) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #177=(CWidgetStyle "Label 13" 1 (LOGPEN 0 0 16763904) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #178=(CWidgetStyle "Label 14" 1 (LOGPEN 0 0 39168) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #179=(CWidgetStyle "Label 15" 1 (LOGPEN 0 0 16750848) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #180=(CWidgetStyle "Marker" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #181=(CWidgetStyle "Marker Label" 1 (LOGPEN 0 0 0) 3 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #182=(CWidgetStyle "Shape 6" 1 (LOGPEN 0 0 0) 3 1 (LOGBRUSH 0 128 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #183=(CWidgetStyle "Shape 7" 1 (LOGPEN 0 0 16711680) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #184=(CWidgetStyle "Shape 8" 1 (LOGPEN 0 0 3381504) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #185=(CWidgetStyle "Label 16" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #186=(CWidgetStyle "Shape 9" 1 (LOGPEN 0 0 16737792) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #187=(CWidgetStyle "Label 17" 1 (LOGPEN 0 0 16737792) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #188=(CWidgetStyle "Label 18" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #189=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #190=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #191=(CWidgetStyle "Label 19" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #192=(CWidgetStyle "Shape 11" 1 (LOGPEN 0 0 153) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #193=(CWidgetStyle "Shape 12" 1 (LOGPEN 0 0 10040064) 4 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #194=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #195=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #196=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #197=(CWidgetStyle "RBS Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #198=(CWidgetStyle "Label 22" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 32768 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #199=(CWidgetStyle "Label 23" 1 (LOGPEN 0 0 3381504) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32768 1 5 "@N (@S)" 0) 
COMMENT          #200=(CWidgetStyle "Shape 14" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 14" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 9 "Shape 1" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 20") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "RBS Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Position Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 9" 1 "Label 16") 68 
COMMENT          (CShapeMapEntry 0 "Shape 8" 1 "Label 23") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 47.8065 47.8065 39 39 0.1 
COMMENT         -11477) 1 1 0 1 1 
COMMENT        (mapper: 18.2666 -17.3616 47.8065 47.8065 0.01 10 -1 5986 5986 1 0 0) 
COMMENT        #201=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 1179544886 100) 
COMMENT              (CObjectList 
COMMENT               #202=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -1290 100) 
COMMENT                     (CObjectList 
COMMENT                      #203=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #123# 28205236 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27523 
COMMENT                             6.27323 1 0.0214037) 0.104798) 
COMMENT                      #204=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #120# 28160812 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 75 28 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "p1168hIL6mCRE-C/EBP-luc+" "@N" 1 0 
COMMENT                            0.871165 0 -10 17.6748 1.56756 Nil) 
COMMENT                      #205=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #119# 27497540 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 87 32 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "5986 bp" "@L bp" 16 
COMMENT                            0 -0.871165 0 -10.8 5.04345 1.81746 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #206=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -1374 100) 
COMMENT                     (CObjectList 
COMMENT                      #207=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -208 100) 
COMMENT                            (CObjectList 
COMMENT                             #208=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #121# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.22567 4.95798 1 0.082322) 0.8 1.8 0) 
COMMENT                             #209=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #195# 27622908 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 55 21 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "LUCm" "@N" 1 
COMMENT                                   11.3507 -7.45043 0 -10 2.79435 1.15863 #208#) 
COMMENT                             #210=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #14# 0) 1 2 0 0 #121# 1267 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    0.920123 1.82243 1 0.082322) 0.8 1.8 1) 
COMMENT                             #211=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #195# 22125724 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 55 21 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ampicillin" 
COMMENT                                   "@N" 1 -14.2618 1.71525 0 -10 4.06657 1.15863 
COMMENT                                   #210#)) (CObjectList)) 
COMMENT                      #212=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -124 100) 
COMMENT                            (CObjectList 
COMMENT                             #213=(CLine 
COMMENT                                   (CWidget 0 (3 #8# 0) 1 2 0 0 #124# 540894580 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.835356 
COMMENT                                   5.56895 6.2407) 
COMMENT                             #214=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #196# 28369308 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 55 21 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "5' FR of hIL6" 
COMMENT                                   "@N" 1 7.51158 11.9539 0 -10 5.58869 1.15863 
COMMENT                                   #213#)) (CObjectList)) 
COMMENT                      #215=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -1207 100) 
COMMENT                            (CObjectList 
COMMENT                             #216=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #189# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    5.00723 5.56895 1 0.082322) 0.8 1.8 0) 
COMMENT                             #217=(CLabel (CWidget 0 (0 0) 1 2 0 0 #195# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 55 21 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hIL6 promoter mutant" "@N" 1 15.1902 6.96932 
COMMENT                                   0 -10 9.40536 1.15863 #216#)) (CObjectList)) 
COMMENT                      #218=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -208 100) 
COMMENT                            (CObjectList 
COMMENT                             #219=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #13# 0) 1 2 0 0 #133# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    2.38834 2.81067 1 0.082322) 0.8 1.8 1) 
COMMENT                             #220=(CLabel (CWidget 0 (0 0) 1 2 0 0 #195# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 55 21 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -8.09245 -10.5315 0 -10 3.93026 1.15863 
COMMENT                                   #219#) 
COMMENT                             #221=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #133# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    0.304961 0.782838 1 0.082322) 0.8 1.8 0) 
COMMENT                             #222=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #195# 27496988 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 55 21 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "f1 ori" "@N" 1 
COMMENT                                   -7.35899 10.5315 0 -10 2.18095 1.15863 #221#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #223=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -374 100) 
COMMENT                            (CObjectList 
COMMENT                             #224=(CLine 
COMMENT                                   (CWidget 0 (3 #11# 0) 1 2 0 0 #124# 541676622 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.835356 
COMMENT                                   3.03284 3.1345) 
COMMENT                             #225=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #195# 1179544886 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 55 21 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA late" "@N" 1 -4.64588 -15.8692 0 
COMMENT                                   -10 6.99722 1.15863 #224#) 
COMMENT                             #226=(CLine 
COMMENT                                   (CWidget 0 (3 #12# 0) 1 2 0 0 #124# 574449012 
COMMENT                                    100) (LOGPEN 0 6 16711680) 8 0.670712 
COMMENT                                   3.03284 3.1345) 
COMMENT                             #227=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #195# 21878516 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 55 21 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 -5.11521 -14.5034 0 
COMMENT                                   -10 7.54246 1.15863 #226#) 
COMMENT                             #228=(CLine 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #124# 
COMMENT                                    1702060386 100) (LOGPEN 0 6 16711680) 8 
COMMENT                                   0.835356 0.00733583 0.168724) 
COMMENT                             #229=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #195# 16777215 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 55 21 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "synthetic polyA" "@N" 1 -4.49535 14.3152 0 
COMMENT                                   -10 6.61101 1.15863 #228#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #230=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -624 100) 
COMMENT                     (CObjectList 
COMMENT                      #231=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil -764 100) 
COMMENT                            (CObjectList 
COMMENT                             #232=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 5.16652 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #233=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Eco}RV (1057)" "@N (@S)" 5 14.1631 
COMMENT                                   4.73972 0 -10 5.42966 1.04504 #232#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #234=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -764 100) 
COMMENT                            (CObjectList 
COMMENT                             #235=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 4.99361 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #236=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Hin}dIII (1222)" "@N (@S)" 5 14.4746 
COMMENT                                   2.85097 0 -10 5.42966 1.04504 #235#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #237=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -1041 100) 
COMMENT                            (CObjectList 
COMMENT                             #238=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 6.24594 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #239=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 27783868 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Xma}I (27)" "@N (@S)" 5 2.45207 14.2881 
COMMENT                                   0 -10 3.83939 1.04504 #238#)) (CObjectList)) 
COMMENT                      #240=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -624 100) 
COMMENT                            (CObjectList 
COMMENT                             #241=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 6.26795 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #242=(CLabel (CWidget 0 (0 0) 1 2 0 0 #185# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Kpn}I (6)" "@N (@S)" 5 1.79722 15.361 0 
COMMENT                                   -10 3.15784 1.04504 #241#)) (CObjectList)) 
COMMENT                      #243=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #6# 0) 1 2 0 0 Nil -124 100) 
COMMENT                            (CObjectList 
COMMENT                             #244=(CScratch 
COMMENT                                   (CWidget 1 (1 #6# 1) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 0.175012 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #245=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 28328028 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Not}I (5820)" "@N (@S)" 5 -4.38402 
COMMENT                                   12.9284 0 -10 4.38462 1.04504 #244#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #246=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #7# 0) 1 2 0 0 Nil -208 100) 
COMMENT                            (CObjectList 
COMMENT                             #247=(CScratch 
COMMENT                                   (CWidget 1 (1 #7# 1) 1 2 0 0 #186# 0 100) 
COMMENT                                   (LOGPEN 0 0 16737792) 1 1 2.94272 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #248=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #185# 14352960 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Sal}I (3179)" "@N (@S)" 5 -4.8949 
COMMENT                                   -11.8136 0 -10 4.20288 1.04504 #247#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #249=(CGroupWidget 
COMMENT                            (CWidget 2 (10 #2# 0) 1 2 0 0 Nil -676 100) 
COMMENT                            (CObjectList 
COMMENT                             #250=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #184# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 1.35713 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #251=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #199# 7249768 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Pvu}I (4692)" "@N (@S)" 5 -14.4243 
COMMENT                                   3.18565 0 -10 4.45278 1.04504 #250#) 
COMMENT                             #252=(CScratch 
COMMENT                                   (CWidget 2 (1 #2# 2) 1 2 0 0 #184# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 0.260946 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #253=(CLabel (CWidget 0 (0 0) 1 2 0 0 #199# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Pvu}I (5738)" "@N (@S)" 5 -5.2888 
COMMENT                                   12.0019 0 -10 4.45278 1.04504 #252#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #254=(CGroupWidget 
COMMENT                            (CWidget 3 (10 #0# 0) 1 2 0 0 Nil -1041 100) 
COMMENT                            (CObjectList 
COMMENT                             #255=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #184# 641 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 6.2407 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #256=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #199# 21145588 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Bam}HI (32)" "@N (@S)" 5 3.10465 
COMMENT                                   13.2151 0 -10 4.43006 1.04504 #255#) 
COMMENT                             #257=(CScratch 
COMMENT                                   (CWidget 2 (1 #0# 2) 1 2 0 0 #184# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 5.17805 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #258=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #199# 28287360 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Bam}HI (1046)" "@N (@S)" 5 13.8543 
COMMENT                                   5.79177 0 -10 5.36151 1.04504 #257#) 
COMMENT                             #259=(CScratch 
COMMENT                                   (CWidget 3 (1 #0# 3) 1 2 0 0 #184# 0 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 1 2.949 1.9 0.082322 1) 
COMMENT                             #260=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #199# 7249768 100) 
COMMENT                                   (LOGPEN 0 0 3381504) 1 
COMMENT                                   (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 32768 
COMMENT                                   "{\\i Bam}HI (3173)" "@N (@S)" 5 -5.22695 
COMMENT                                   -12.9912 0 -10 5.36151 1.04504 #259#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #261=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil -1207 100) 
COMMENT                     (CObjectList 
COMMENT                      #262=(CLabel (CWidget 0 (15 #17# 0) 1 2 0 0 #146# 0 100) 
COMMENT                            (LOGPEN 0 27648928 27501788) 27648896 
COMMENT                            (LOGFONT 75 28 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 4497" 
COMMENT                            "LMBP 4497" 65535 0 -2.61471 0 -10 7.11082 1.56756 
COMMENT                            Nil) 
COMMENT                      #263=(CLabel (CWidget 0 (15 #18# 0) 1 2 0 0 #146# 235 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 50 19 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 23-OCT-2007" 
COMMENT                            "Last modified: 23-OCT-2007" 65535 -0.397435 
COMMENT                            -4.12078 0 -10 11.1092 1.04504 Nil)) (CObjectList)) 
COMMENT               #264=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -208 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 27817920 
COMMENT       (CStringList) 21193392 1 (CObList)))
FEATURES             Location/Qualifiers
     misc_feature    32..672
                     /vntifkey="21"
                     /label=5'\FR\of\hIL6
                     /note="5' flanking region of the human interleukin 6 gene"
     promoter        673..1208
                     /vntifkey="29"
                     /label=hIL6\promoter\mutant
                     /note="mutated human interleukin 6 promoter"
     CDS             1256..2908
                     /vntifkey="4"
                     /label=LUCm
                     /note="mutated firefly luciferase coding sequence"
     terminator      2996..3092
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      complement(2996..3092)
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(3305..3707)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(4248..5108)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      5240..5695
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     terminator      complement(5826..5979)
                     /vntifkey="43"
                     /label=synthetic\polyA
                     /note="synthetic polyA signal"
BASE COUNT     1587 a      1429 c      1433 g      1537 t 
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg ggatcctcct gcaagagaca ccatcctgag 
       61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag 
      121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt 
      181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg 
      241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac 
      301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag 
      361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac 
      421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg 
      481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc 
      541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg 
      601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag 
      661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga 
      721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg 
      781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa 
      841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac 
      901 tctttgtcaa gacatgccaa agtgctgagt cactaataaa agaaaaaaag aaagtaaagg 
      961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc 
     1021 tagttgtgtc ttgcgatgct aaagggatcc acagatatca atcttaataa ggtttccaat 
     1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggat 
     1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc 
     1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaag ccaccatgga 
     1261 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc 
     1321 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt 
     1381 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg 
     1441 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag 
     1501 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt 
     1561 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca 
     1621 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa 
     1681 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca 
     1741 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt 
     1801 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac 
     1861 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc 
     1921 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc 
     1981 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt 
     2041 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat 
     2101 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga 
     2161 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga 
     2221 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct 
     2281 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt 
     2341 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct 
     2401 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt 
     2461 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga 
     2521 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat 
     2581 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc 
     2641 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc 
     2701 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt 
     2761 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt 
     2821 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc 
     2881 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc 
     2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 
     3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 
     3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 
     3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc 
     3181 gaccgatgcc cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga 
     3241 ctatcgtcgc cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg 
     3301 cagcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 
     3361 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 
     3421 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 
     3481 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 
     3541 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 
     3601 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 
     3661 tcgggaagcg tggcgctttc tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc 
     3721 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 
     3781 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 
     3841 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 
     3901 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 
     3961 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 
     4021 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 
     4081 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 
     4141 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 
     4201 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 
     4261 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 
     4321 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 
     4381 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 
     4441 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 
     4501 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 
     4561 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 
     4621 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 
     4681 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 
     4741 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 
     4801 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 
     4861 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 
     4921 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 
     4981 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 
     5041 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 
     5101 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 
     5161 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 
     5221 ccccgaaaag tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg 
     5281 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc 
     5341 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc 
     5401 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt 
     5461 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag 
     5521 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg 
     5581 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag 
     5641 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgtttac aatttcccat 
     5701 tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta 
     5761 cgccagccca agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg 
     5821 gccgcaataa aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga 
     5881 tagtactaac atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg 
     5941 ctgtccccag tgcaagtgca ggtgccagaa catttctcta tcgata 
//
