LOCUS       p1168hIL6m5AP1-luc+        5990 bp    DNA     circular     23-OCT-2007
DEFINITION  LMBP 4500.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4500
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 12-NOV-2012
            
COMMENT     VNTNAME|p1168hIL6m5AP1-luc+|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "p1168hIL6m5AP1-luc+" 0 5990 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "BamHI" "HindIII" "KpnI" "NcoI" "NheI" "NotI" "PstI" "PvuI" 
COMMENT         "SalI" "XmaI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 649 648 1 5990 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 2 32 0 3173 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 1 1222 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 6 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 1 1255 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "NheI" "gctagc" 2 0 2 22 0 984 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 5824 0 0 "") 
COMMENT        #6=(CRSite (CStringList) "PstI" "ctgcag" 6 0 1 930 0 0 "") 
COMMENT        #7=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 3 3184 0 4696 0 5742 0 0 "") 
COMMENT        #8=(CRSite (CStringList) "SalI" "gtcgac" 2 0 0 0 "") 
COMMENT        #9=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 27 0 0 "")) 
COMMENT       (CObList 
COMMENT        #10=(CFSignal (CObList) "5' FR of hIL6" 21 0 0 32 672 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "32..672") 
COMMENT        #11=(CFSignal (CObList) "hIL6 promoter mutant" 29 0 0 673 1208 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "673..1208") 
COMMENT        #12=(CFSignal (CObList) "LUCm" 4 0 0 1256 2908 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1256..2908") 
COMMENT        #13=(CFSignal (CObList) "SV40 polyA late" 43 0 0 2996 3092 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2996..3092") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA early" 43 0 1 2996 3092 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(2996..3092)") 
COMMENT        #15=(CFSignal (CObList) "pMB1 ori" 33 0 1 3309 3711 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3309..3711)") 
COMMENT        #16=(CFSignal (CObList) "ampicillin resistance" 4 0 1 4252 5112 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4252..5112)") 
COMMENT        #17=(CFSignal (CObList) "f1 ori" 33 0 0 5244 5699 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "5244..5699") 
COMMENT        #18=(CFSignal (CObList) "synthetic polyA" 43 0 1 5830 5983 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5830..5983)")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #19=(CFSignal (CObList) "LMBP 4500" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #20=(CFSignal (CObList) "Last modified: 12-NOV-2012" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
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COMMENT                    "p1168hIL6m5AP1-luc+" 2) 5 "" 0 4) 
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COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
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COMMENT                          "DNA Plasmid 'p1168hIL6m5AP1-luc+'" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4500." 
COMMENT                          1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/23/07 04:10" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 5990 bp" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 17-OCT-2006" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "BCCM/GeneCorner Plasmid Collection" 1))) "Original Author") 
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COMMENT                                 "" 1))) "LMBP 4500") 
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COMMENT                                 "" 1))) "Last modified: 12-NOV-2012"))) 
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COMMENT                                        "Start: 1256  End: 2908" 1) 
COMMENT                                   #47=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "mutated firefly luciferase coding sequence" 
COMMENT                                        1) 
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COMMENT                                   #49=(CLinePar 
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COMMENT                                        "   1256..2908" 1))) "LUCm") 
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COMMENT                                   #52=(CLinePar 
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COMMENT                                        "antibiotic resistance gene; coding sequence" 
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COMMENT                                        "   complement(4252..5112)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (2 total)") 
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COMMENT                                        "Start: 32    End: 672 " 1) 
COMMENT                                   #58=(CLinePar 
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COMMENT                                        "5' flanking region of the human interleukin 6 gene" 
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COMMENT                                        "Start: 673   End: 1208" 1) 
COMMENT                                   #64=(CLinePar 
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COMMENT                                        "mutated human interleukin 6 promoter" 1) 
COMMENT                                   #65=(CLinePar 
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COMMENT                                 "hIL6 promoter mutant"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3309  End: 3711 (Complementary)" 
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COMMENT                                   #70=(CLinePar 
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COMMENT                                        "origin plasmid" 1) 
COMMENT                                   #71=(CLinePar 
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COMMENT                                        "   complement(3309..3711)" 1))) 
COMMENT                                 "pMB1 ori") 
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COMMENT                                        "Start: 5244  End: 5699" 1) 
COMMENT                                   #75=(CLinePar 
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COMMENT                                        "phage f1 origin" 1) 
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COMMENT                                   #77=(CLinePar 
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COMMENT                                        "   5244..5699" 1))) "f1 ori"))) 
COMMENT                          "Replication Origin (2 total)") 
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COMMENT                                        "polyA signal of the SV40 late region" 1) 
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COMMENT                                        "   2996..3092" 1))) "SV40 polyA late") 
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COMMENT                                   #86=(CLinePar 
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COMMENT                                        "polyA signal of the SV40 early region" 
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COMMENT                                 "SV40 polyA early") 
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COMMENT                                        "   complement(5830..5983)" 1))) 
COMMENT                                 "synthetic polyA"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                          5 "GGATCC" "CCTAGG") 
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FEATURES             Location/Qualifiers
     misc_feature    32..672
                     /vntifkey="21"
                     /label=5'\FR\of\hIL6
                     /note="5' flanking region of the human interleukin 6 gene"
     promoter        673..1208
                     /vntifkey="29"
                     /label=hIL6\promoter\mutant
                     /note="mutated human interleukin 6 promoter"
     CDS             1256..2908
                     /vntifkey="4"
                     /label=LUCm
                     /note="mutated firefly luciferase coding sequence"
     terminator      2996..3092
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     terminator      complement(2996..3092)
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     rep_origin      complement(3309..3711)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(4252..5112)
                     /vntifkey="4"
                     /label=ampicillin\resistance
                     /note="antibiotic resistance gene; coding sequence"
     rep_origin      5244..5699
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     terminator      complement(5830..5983)
                     /vntifkey="43"
                     /label=synthetic\polyA
                     /note="synthetic polyA signal"
BASE COUNT     1587 a      1432 c      1434 g      1537 t 
ORIGIN
        1 ggtaccgagc tcttacgcgt gctagcccgg ggatcctcct gcaagagaca ccatcctgag 
       61 ggaagagggc ttctgaacca gcttgaccca ataagaaatt cttgggtgcc gacgcggaag 
      121 cagattcaga gcctagagcc gtgcctgcgt ccgtagtttc cttctagctt cttttgattt 
      181 caaatcaaga cttacaggga gagggagcga taaacacaaa ctctgcaaga tgccacaagg 
      241 tcctcctttg acatccccaa caaagaggtg agtagtattc tccccctttc tgccctgaac 
      301 caagtgggct tcagtaattt cagggctcca ggagacctgg ggcccatgca ggtgccccag 
      361 tgaaacagtg gtgaagagac tcagtggcaa tggggagagc actggcagca caaggcaaac 
      421 ctctggcaca gagagcaaag tcctcactgg gaggattccc aaggggtcac ttgggagagg 
      481 gcagggcagc agccaacctc ctctaagtgg gctgaagcag gtgaagaaag tggcagaagc 
      541 cacgcggtgg caaaaaggag tcacacactc cacctggaga cgccttgaag taactgcacg 
      601 aaatttgagg atggccaggc agttctacaa cagccgctca cagggagagc cagaacacag 
      661 aagaactcag atgactggta gtattacctt cttcataatc ccaggcttgg ggggctgcga 
      721 tggagtcaga ggaaactcag ttcagaacat ctttggtttt tacaaataca aattaactgg 
      781 aacgctaaat tctagcctgt taatctggtc actgaaaaaa aatttttttt ttttcaaaaa 
      841 acatagcttt agcttatttt ttttctcttt gtaaaacttc gtgcatgact tcagctttac 
      901 tctttgtcaa gacatgccaa agtgctgcag cactaataaa agaaaaaaag aaagtaaagg 
      961 aagagtggtt ctgcttctta gcgctagcct caatgacgac ctaagctgca cttttccccc 
     1021 tagttgtgtc ttgcgatgct aaaggacgtc acattgcaca atcttaataa ggtttccaat 
     1081 cagccccacc cgctctggcc ccaccctcac cctccaacaa agatttatca aatgtgggat 
     1141 tttcccatga gtctcaatat tagagtctca acccccaata aatataggac tggagatgtc 
     1201 tgaggctcat tctgccctcg aagcttggca ttccggtact gttggtaaag ccaccatgga 
     1261 agacgccaaa aacataaaga aaggcccggc gccattctat ccgctggaag atggaaccgc 
     1321 tggagagcaa ctgcataagg ctatgaagag atacgccctg gttcctggaa caattgcttt 
     1381 tacagatgca catatcgagg tggacatcac ttacgctgag tacttcgaaa tgtccgttcg 
     1441 gttggcagaa gctatgaaac gatatgggct gaatacaaat cacagaatcg tcgtatgcag 
     1501 tgaaaactct cttcaattct ttatgccggt gttgggcgcg ttatttatcg gagttgcagt 
     1561 tgcgcccgcg aacgacattt ataatgaacg tgaattgctc aacagtatgg gcatttcgca 
     1621 gcctaccgtg gtgttcgttt ccaaaaaggg gttgcaaaaa attttgaacg tgcaaaaaaa 
     1681 gctcccaatc atccaaaaaa ttattatcat ggattctaaa acggattacc agggatttca 
     1741 gtcgatgtac acgttcgtca catctcatct acctcccggt tttaatgaat acgattttgt 
     1801 gccagagtcc ttcgataggg acaagacaat tgcactgatc atgaactcct ctggatctac 
     1861 tggtctgcct aaaggtgtcg ctctgcctca tagaactgcc tgcgtgagat tctcgcatgc 
     1921 cagagatcct atttttggca atcaaatcat tccggatact gcgattttaa gtgttgttcc 
     1981 attccatcac ggttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt 
     2041 cgtcttaatg tatagatttg aagaagagct gtttctgagg agccttcagg attacaagat 
     2101 tcaaagtgcg ctgctggtgc caaccctatt ctccttcttc gccaaaagca ctctgattga 
     2161 caaatacgat ttatctaatt tacacgaaat tgcttctggt ggcgctcccc tctctaagga 
     2221 agtcggggaa gcggttgcca agaggttcca tctgccaggt atcaggcaag gatatgggct 
     2281 cactgagact acatcagcta ttctgattac acccgagggg gatgataaac cgggcgcggt 
     2341 cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat ctggataccg ggaaaacgct 
     2401 gggcgttaat caaagaggcg aactgtgtgt gagaggtcct atgattatgt ccggttatgt 
     2461 aaacaatccg gaagcgacca acgccttgat tgacaaggat ggatggctac attctggaga 
     2521 catagcttac tgggacgaag acgaacactt cttcatcgtt gaccgcctga agtctctgat 
     2581 taagtacaaa ggctatcagg tggctcccgc tgaattggaa tccatcttgc tccaacaccc 
     2641 caacatcttc gacgcaggtg tcgcaggtct tcccgacgat gacgccggtg aacttcccgc 
     2701 cgccgttgtt gttttggagc acggaaagac gatgacggaa aaagagatcg tggattacgt 
     2761 cgccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga gttgtgtttg tggacgaagt 
     2821 accgaaaggt cttaccggaa aactcgacgc aagaaaaatc agagagatcc tcataaaggc 
     2881 caagaagggc ggaaagatcg ccgtgtaatt ctagagtcgg ggcggccggc cgcttcgagc 
     2941 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa 
     3001 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa 
     3061 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg 
     3121 ggaggttttt taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtc 
     3181 gatcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 
     3241 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg 
     3301 ccggcagcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg 
     3361 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa 
     3421 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc 
     3481 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc 
     3541 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag 
     3601 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct 
     3661 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta 
     3721 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc 
     3781 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc 
     3841 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt 
     3901 gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct 
     3961 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc 
     4021 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca 
     4081 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta 
     4141 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa 
     4201 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg 
     4261 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg 
     4321 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc 
     4381 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc 
     4441 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa 
     4501 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc 
     4561 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg 
     4621 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc 
     4681 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat 
     4741 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg 
     4801 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc 
     4861 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg 
     4921 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat 
     4981 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg 
     5041 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg 
     5101 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct 
     5161 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac 
     5221 atttccccga aaagtgccac ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt 
     5281 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc 
     5341 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg 
     5401 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta 
     5461 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt 
     5521 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat 
     5581 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa 
     5641 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttc 
     5701 ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct 
     5761 attacgccag cccaagctac catgataagt aagtaatatt aaggtacggg aggtacttgg 
     5821 agcggccgca ataaaatatc tttattttca ttacatctgt gtgttggttt tttgtgtgaa 
     5881 tcgatagtac taacatacgc tctccatcaa aacaaaacga aacaaaacaa actagcaaaa 
     5941 taggctgtcc ccagtgcaag tgcaggtgcc agaacatttc tctatcgata 
//
