ID pEF6-eIF2-A-Myc-His standard; circular DNA; ; 6716 BP. DE LMBP 4506. CC Accession number: LMBP 4506 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 27-APR-2011 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 386..1529 FT /vntifkey="29" FT /label=hEF1a\promoter FT intron 582..1520 FT /vntifkey="15" FT /label=intron\hEF1a\promoter FT promoter 1546..1562 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT CDS 1611..2555 FT /vntifkey="4" FT /label=eIF2\alpha\subunit FT /note="alpha-subunit of the eukaryotic translation initiation factor 2" FT CDS 2556..2567 FT /vntifkey="4" FT /label=His4 FT /note="four His codons" FT misc_marker 2598..2627 FT /vntifkey="22" FT /label=Myc\epitope FT /note="Myc epitope" FT misc_marker 2643..2660 FT /vntifkey="22" FT /label=His-tag FT /note="polyhistidine (6 x His) tag" FT terminator 2689..2913 FT /vntifkey="43" FT /label=BGH\polyA FT rep_origin 2959..3387 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT promoter 3392..3673 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin 3630..3705 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 3778..3843 FT /vntifkey="30" FT /label=synthetic\EM7\promoter FT CDS 3844..4242 FT /vntifkey="4" FT /label=blasticidin\resistance FT terminator 4400..4474 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(4400..4474) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(4788..5179) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5720..6580) FT /vntifkey="4" FT /label=ampicillin\resistance FT promoter complement(4613..4696) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 6716 BP; 1599 A; 1630 C; 1775 G; 1712 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 300 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 360 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 420 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 480 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 540 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 600 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 660 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 720 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 780 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 840 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 900 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 960 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1020 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1080 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1140 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1200 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1260 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1320 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1380 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1440 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1500 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1560 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccgt atgccgggtc 1620 taagttgtag attttatcaa cacaaatttc ctgaggtgga agatgtagtg atggtgaatg 1680 tcagatccat tgctgaaatg ggggcttatg tcagcttgct ggaatacaac aacattgaag 1740 gcatgattct tcttagtgaa ttatccagaa ggcgtatccg ttctatcaac aaactcatcc 1800 gaattggcag gaatgagtgt gtggttgtca ttagggtgga caaagaaaaa ggatatattg 1860 atttgtcaaa aagaagagtt tctccagagg aagcaatcaa atgtgaagac aaattcacaa 1920 aatccaaaac tgtttatagc attcttcgtc atgttgctga ggtgttagaa tacaccaagg 1980 atgagcagct ggaaagccta ttccagagga ctgcctgggt ctttgatgac aagtacaaga 2040 gacctggata tggtgcctat gatgcattta agcatgcagt ctcagaccca tctattttgg 2100 atagtttaga tttgaatgaa gatgaacggg aagtactcat taataatatt aataggcgct 2160 tgaccccaca ggctgtcaaa attcgagcag atattgaagt ggcttgttat ggttatgaag 2220 gcattgatgc tgtaaaagaa gccctaagag caggtttgaa ttgttctaca gaaaacatgc 2280 ccattaagat taatctaata gctcctcctc ggtatgtaat gactacgaca accctggaga 2340 gaacagaagg cctttctgtc ctcagtcaag ctatggctgt tatcaaagag aagattgagg 2400 aaaagagggg tgtgttcaat gttcaaatgg agcccaaagt ggtcacagat acagatgaga 2460 ctgaacttgc gaggcagatg gagaggcttg aaagagaaaa tgccgaagtg gatggagatg 2520 atgatgcaga agaaatggaa gccaaagctg aagatcacca ccaccacctg agcggccgcg 2580 actctagagg gcccttcgaa caaaaactca tctcagaaga ggatctgaat atgcataccg 2640 gtcatcatca ccatcaccat tgagtttaaa cccgctgatc agcctcgact gtgccttcta 2700 gttgccagcc atctgttgtt tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca 2760 ctcccactgt cctttcctaa taaaatgagg aaattgcatc gcattgtctg agtaggtgtc 2820 attctattct ggggggtggg gtggggcagg acagcaaggg ggaggattgg gaagacaata 2880 gcaggcatgc tggggatgcg gtgggctcta tggcttctga ggcggaaaga accagctggg 2940 gctctagggg gtatccccac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg 3000 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct 3060 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc 3120 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg 3180 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt 3240 ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg 3300 tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc 3360 tgatttaaca aaaatttaac gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg 3420 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 3480 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct 3540 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc 3600 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga 3660 ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg 3720 cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc agcacgtgtt 3780 gacaattaat catcggcata gtatatcggc atagtataat acgacaaggt gaggaactaa 3840 accatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc aacggctaca 3900 atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct ctctagcgac 3960 ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg tgcagaactc 4020 gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc 4080 ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt gcttctcgat 4140 ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac ggcagttggg 4200 attcgtgaat tgctgccctc tggttatgtg tgggagggct aagcacttcg tggccgagga 4260 gcaggactga cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg 4320 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct 4380 ggagttcttc gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa 4440 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc 4500 caaactcatc aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc 4560 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 4620 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac 4680 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca 4740 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc 4800 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc 4860 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc 4920 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 4980 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 5040 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 5100 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 5160 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 5220 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 5280 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 5340 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 5400 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 5460 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 5520 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 5580 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 5640 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 5700 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 5760 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 5820 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 5880 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 5940 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 6000 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 6060 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 6120 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 6180 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 6240 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 6300 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 6360 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 6420 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 6480 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 6540 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 6600 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 6660 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 6716 //