LOCUS       pEF6-hPKR-D251NK        7547 bp    DNA     circular     10-JAN-2017
DEFINITION  LMBP 4507.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pEF6-hPKR-D251NK296R-MycHis|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTREPLTYPE|Virus
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-hPKR-D251NK296R-MycHis" 0 7547 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7547 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "hPKRm" 4 0 0 1741 3393 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1617..3269") 
COMMENT        #1=(CFSignal (CObList) "blasticidin resistance" 4 0 0 4664 5062 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "4540..4938") 
COMMENT        #2=(CFSignal (CObList) "ampicillin resistance" 4 0 1 6551 7411 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(6416..7276)") 
COMMENT        #3=(CFSignal (CObList) "His-tag" 22 0 0 3463 3480 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #4=(CFSignal (CObList) "Myc epitope" 22 0 0 3418 3447 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #5=(CFSignal (CObList) "SV40 early promoter" 29 0 0 4212 4493 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #6=(CFSignal (CObList) "SV40 ori" 33 0 0 4450 4525 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #7=(CFSignal (CObList) "synthetic EM7 promoter" 30 0 0 4598 4663 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #8=(CFSignal (CObList) "hEF1 promoter" 29 0 0 510 1653 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "") 
COMMENT        #9=(CFSignal (CObList) "intron hEF1 promoter" 15 0 0 706 1644 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #10=(CFSignal (CObList) "T7 promoter" 30 0 0 1670 1686 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #11=(CFSignal (CObList) "f1 ori" 33 0 0 3779 4211 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #12=(CFSignal (CObList) "pMB1 ori" 33 0 1 5608 6010 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #13=(CFSignal (CObList) "BGH polyA" 43 0 0 3509 3733 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #14=(CFSignal (CObList) "SV40 polyA early" 43 0 0 5220 5294 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #15=(CFSignal (CObList) "SV40 polyA late" 43 0 1 5220 5294 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT                    "pEF6-hPKR-D251NK296R-MycHis" 2) 5 "" 0 4) 
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COMMENT                          "DNA Plasmid Phage Virus 'pEF6-hPKR-D251NK296R-MycHis'" 
COMMENT                          1) 
COMMENT                     #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4507." 
COMMENT                          1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 01/10/17 12:35" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 7547 bp" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 19-MAR-1980" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #37=(CLinePar 
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COMMENT                                        "   1617..3269" 1))) "hPKRm") 
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COMMENT                                   #41=(CLinePar 
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COMMENT                                 "blasticidin resistance") 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "   complement(6416..7276)" 1))) 
COMMENT                                 "ampicillin resistance"))) "CDS (3 total)") 
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COMMENT                                 "intron hEF1 promoter"))) "Intron (1 total)") 
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COMMENT                          "Promoter Eukaryotic (2 total)") 
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COMMENT                                 "synthetic EM7 promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                          "Replication Origin (3 total)") 
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COMMENT                                   "SV40 early promoter" "@N" 1 -11.3907 
COMMENT                                   -12.1963 0 -10 8.35465 1.16519 #226#) 
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COMMENT                                   "synthetic EM7 promoter" "@N" 1 -15.747 
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COMMENT                                   "@N" 1 15.969 1.74233 0 -10 4.88388 1.16519 
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FEATURES             Location/Qualifiers
     CDS             1741..3393
                     /vntifkey="4"
                     /label=hPKRm
     CDS             4664..5062
                     /vntifkey="4"
                     /label=blasticidin\resistance
     CDS             complement(6551..7411)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_marker     3463..3480
                     /vntifkey="22"
                     /label=His-tag
     misc_marker     3418..3447
                     /vntifkey="22"
                     /label=Myc\epitope
     promoter        4212..4493
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4450..4525
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        4598..4663
                     /vntifkey="30"
                     /label=synthetic\EM7\promoter
     promoter        510..1653
                     /vntifkey="29"
                     /label=hEF1\promoter
     intron          706..1644
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     promoter        1670..1686
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      3779..4211
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(5608..6010)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      3509..3733
                     /vntifkey="43"
                     /label=BGH\polyA
     terminator      5220..5294
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5220..5294)
                     /vntifkey="43"
                     /label=SV40\polyA\late
BASE COUNT     1927 a      1772 c      1915 g      1933 t 
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtcgact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgcctc gtgaggctcc 
      481 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg 
      541 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc 
      601 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc 
      661 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt 
      721 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc 
      781 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt 
      841 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg 
      901 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat 
      961 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat 
     1021 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc 
     1081 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg 
     1141 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc 
     1201 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg 
     1261 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg 
     1321 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca 
     1381 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc 
     1441 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt 
     1501 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc 
     1561 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt 
     1621 caaagttttt ttcttccatt tcaggtgtcg tgaggaatta gcttggtact aatacgactc 
     1681 actataggga gacccaagct ggctaggtaa gcttggtacc gagctcggat ccgtcgacta 
     1741 atggctggtg atctttcagc aggtttcttc atggaggaac ttaatacata ccgtcagaag 
     1801 cagggagtag tacttaaata tcaagaactg cctaattcag gacctccaca tgataggagg 
     1861 tttacatttc aagttataat agatggaaga gaatttccag aaggtgaagg tagatcaaag 
     1921 aaggaagcaa aaaatgccgc agccaaatta gctgttgaga tacttaataa ggaaaagaag 
     1981 gcagttagtc ctttattatt gacaacaacg aattcttcag aaggattatc catggggaat 
     2041 tacataggcc ttatcaatag aattgcccag aagaaaagac taactgtaaa ttatgaacag 
     2101 tgtgcatcgg gggtgcatgg gccagaagga tttcattata aatgcaaaat gggacagaaa 
     2161 gaatatagta ttggtacagg ttctactaaa caggaagcaa aacaattggc cgctaaactt 
     2221 gcatatcttc agatattatc agaagaaacc tcagtgaaat ctgactacct gtcctctggt 
     2281 tcttttgcta ctacgtgtga gtcccaaagc aactctttag tgaccagcac actcgcttct 
     2341 gaatcatcat ctgaaggtga cttctcagca gatacatcag agataaattc taacagtgac 
     2401 agtttaaaca gttcttcgtt gcttatgaat ggtctcagaa ataatcaaag gaaggcaaaa 
     2461 agatctttgg cacccagatt tgaccttcct aacatgaaag aaacaaagta tactgtggac 
     2521 aagaggtttg gcatggattt taaagaaata gaattaattg gctcaggtgg atttggccaa 
     2581 gttttcaaag caaaacacag aattgacgga aagacttacg ttattcgacg tgttaaatat 
     2641 aataacgaga aggcggagcg tgaagtaaaa gcattggcaa aacttgatca tgtaaatatt 
     2701 gttcactaca atggctgttg ggatggattt gattatgatc ctgagaccag tgatgattct 
     2761 cttgagagca gtgattatga tcctgagaac agcaaaaata gttcaaggtc aaagactaag 
     2821 tgccttttca tccaaatgga attctgtgat aaagggacct tggaacaatg gattgaaaaa 
     2881 agaagaggcg agaaactaga caaagttttg gctttggaac tctttgaaca aataacaaaa 
     2941 ggggtggatt atatacattc aaaaaaatta attcatagag atcttaagcc aagtaatata 
     3001 ttcttagtag atacaaaaca agtaaagatt ggagactttg gacttgtaac atctctgaaa 
     3061 aatgatggaa agcgaacaag gagtaaggga actttgcgat acatgagccc agaacagatt 
     3121 tcttcgcaag actatggaaa ggaagtggac ctctacgctt tggggctaat tcttgctgaa 
     3181 cttcttcatg tatgtgacac tgcttttgaa acatcaaagt ttttcacaga cctacgggat 
     3241 ggcatcatct cagatatatt tgataaaaaa gaaaaaactc ttctacagaa attactctca 
     3301 aagaaacctg aggatcgacc taacacatct gaaatactaa ggaccttgac tgtgtggaag 
     3361 aaaagcccag agaaaaatga acgacacaca tgtgcggccg ctcgaggtca cccattcgaa 
     3421 caaaaactca tctcagaaga ggatctgaat atgcataccg gtcatcatca ccatcaccat 
     3481 tgagtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt 
     3541 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa 
     3601 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg 
     3661 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg 
     3721 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac 
     3781 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct 
     3841 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg 
     3901 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt 
     3961 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca 
     4021 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga 
     4081 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa 
     4141 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac 
     4201 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag 
     4261 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc 
     4321 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag 
     4381 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc 
     4441 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc 
     4501 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg 
     4561 gagcttgtat atccattttc ggatctgatc agcacgtgtt gacaattaat catcggcata 
     4621 gtatatcggc atagtataat acgacaaggt gaggaactaa accatggcca agcctttgtc 
     4681 tcaagaagaa tccaccctca ttgaaagagc aacggctaca atcaacagca tccccatctc 
     4741 tgaagactac agcgtcgcca gcgcagctct ctctagcgac ggccgcatct tcactggtgt 
     4801 caatgtatat cattttactg ggggaccttg tgcagaactc gtggtgctgg gcactgctgc 
     4861 tgctgcggca gctggcaacc tgacttgtat cgtcgcgatc ggaaatgaga acaggggcat 
     4921 cttgagcccc tgcggacggt gccgacaggt gcttctcgat ctgcatcctg ggatcaaagc 
     4981 catagtgaag gacagtgatg gacagccgac ggcagttggg attcgtgaat tgctgccctc 
     5041 tggttatgtg tgggagggct aagcacttcg tggccgagga gcaggactga cacgtgctac 
     5101 gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc gttttccggg 
     5161 acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc gcccacccca 
     5221 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa 
     5281 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt 
     5341 atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg tcatagctgt 
     5401 ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc ggaagcataa 
     5461 agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg ttgcgctcac 
     5521 tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg 
     5581 cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact gactcgctgc 
     5641 gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat 
     5701 ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca 
     5761 ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc 
     5821 atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc 
     5881 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg 
     5941 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta 
     6001 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg 
     6061 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac 
     6121 acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag 
     6181 gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat 
     6241 ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat 
     6301 ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc 
     6361 gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt 
     6421 ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct 
     6481 agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt 
     6541 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc 
     6601 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac 
     6661 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat 
     6721 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg 
     6781 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata 
     6841 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta 
     6901 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt 
     6961 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag 
     7021 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa 
     7081 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc 
     7141 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt 
     7201 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc 
     7261 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta 
     7321 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa 
     7381 taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca 
     7441 tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac 
     7501 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtc 
//
