ID pEF6-hPKR-K296R-MycHis standard; circular DNA; ; 7412 BP. DE LMBP 4509. CC Accession number: LMBP 4509 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 27-NOV-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 386..1529 FT /vntifkey="29" FT /label=hEF1a\promoter FT /note="human elongation factor 1alpha promoter" FT intron 582..1520 FT /vntifkey="15" FT /label=intron\hEF1a\promoter FT /note="human elongation factor 1alpha intron" FT promoter 1546..1562 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT CDS 1617..3269 FT /vntifkey="4" FT /label=mutated\hPKR FT /note="mutated human dsRNA-activated protein kinase coding sequence" FT misc_marker 3294..3323 FT /vntifkey="22" FT /label=Myc\epitope FT /note="Myc epitope" FT misc_marker 3339..3356 FT /vntifkey="22" FT /label=His-tag FT /note="polyhistidine (6 x His) tag" FT terminator 3385..3609 FT /vntifkey="43" FT /label=BGH\polyA FT /note="polyA signal of the bovine growth hormone" FT rep_origin 3655..4083 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT promoter 4088..4369 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin 4326..4401 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 4474..4539 FT /vntifkey="30" FT /label=EM7\promoter FT /note="synthetic prokaryotic EM7 promoter" FT CDS 4540..4938 FT /vntifkey="4" FT /label=blasticidin\resistance FT /note="antibiotic resistance gene; coding sequence" FT terminator 5096..5170 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(5096..5170) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(5484..5875) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(6416..7276) FT /vntifkey="4" FT /label=ampicillin\resistance FT /note="antibiotic resistance gene; coding sequence" FT promoter complement(5309..5392) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 7412 BP; 1883 A; 1744 C; 1888 G; 1897 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 300 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 360 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 420 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 480 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 540 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 600 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 660 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 720 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 780 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 840 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 900 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 960 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1020 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1080 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1140 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1200 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1260 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1320 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1380 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1440 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1500 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1560 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccgt cgactaatgg 1620 ctggtgatct ttcagcaggt ttcttcatgg aggaacttaa tacataccgt cagaagcagg 1680 gagtagtact taaatatcaa gaactgccta attcaggacc tccacatgat aggaggttta 1740 catttcaagt tataatagat ggaagagaat ttccagaagg tgaaggtaga tcaaagaagg 1800 aagcaaaaaa tgccgcagcc aaattagctg ttgagatact taataaggaa aagaaggcag 1860 ttagtccttt attattgaca acaacgaatt cttcagaagg attatccatg gggaattaca 1920 taggccttat caatagaatt gcccagaaga aaagactaac tgtaaattat gaacagtgtg 1980 catcgggggt gcatgggcca gaaggatttc attataaatg caaaatggga cagaaagaat 2040 atagtattgg tacaggttct actaaacagg aagcaaaaca attggccgct aaacttgcat 2100 atcttcagat attatcagaa gaaacctcag tgaaatctga ctacctgtcc tctggttctt 2160 ttgctactac gtgtgagtcc caaagcaact ctttagtgac cagcacactc gcttctgaat 2220 catcatctga aggtgacttc tcagcagata catcagagat aaattctaac agtgacagtt 2280 taaacagttc ttcgttgctt atgaatggtc tcagaaataa tcaaaggaag gcaaaaagat 2340 ctttggcacc cagatttgac cttcctgaca tgaaagaaac aaagtatact gtggacaaga 2400 ggtttggcat ggattttaaa gaaatagaat taattggctc aggtggattt ggccaagttt 2460 tcaaagcaaa acacagaatt gacggaaaga cttacgttat tcgacgtgtt aaatataata 2520 acgagaaggc ggagcgtgaa gtaaaagcat tggcaaaact tgatcatgta aatattgttc 2580 actacaatgg ctgttgggat ggatttgatt atgatcctga gaccagtgat gattctcttg 2640 agagcagtga ttatgatcct gagaacagca aaaatagttc aaggtcaaag actaagtgcc 2700 ttttcatcca aatggaattc tgtgataaag ggaccttgga acaatggatt gaaaaaagaa 2760 gaggcgagaa actagacaaa gttttggctt tggaactctt tgaacaaata acaaaagggg 2820 tggattatat acattcaaaa aaattaattc atagagatct taagccaagt aatatattct 2880 tagtagatac aaaacaagta aagattggag actttggact tgtaacatct ctgaaaaatg 2940 atggaaagcg aacaaggagt aagggaactt tgcgatacat gagcccagaa cagatttctt 3000 cgcaagacta tggaaaggaa gtggacctct acgctttggg gctaattctt gctgaacttc 3060 ttcatgtatg tgacactgct tttgaaacat caaagttttt cacagaccta cgggatggca 3120 tcatctcaga tatatttgat aaaaaagaaa aaactcttct acagaaatta ctctcaaaga 3180 aacctgagga tcgacctaac acatctgaaa tactaaggac cttgactgtg tggaagaaaa 3240 gcccagagaa aaatgaacga cacacatgtg cggccgctcg aggtcaccca ttcgaacaaa 3300 aactcatctc agaagaggat ctgaatatgc ataccggtca tcatcaccat caccattgag 3360 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc 3420 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa 3480 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg 3540 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg 3600 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc 3660 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac 3720 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg 3780 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt 3840 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc 3900 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct 3960 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga 4020 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga 4080 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg 4140 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg 4200 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc 4260 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca 4320 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt 4380 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc 4440 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat 4500 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagcc tttgtctcaa 4560 gaagaatcca ccctcattga aagagcaacg gctacaatca acagcatccc catctctgaa 4620 gactacagcg tcgccagcgc agctctctct agcgacggcc gcatcttcac tggtgtcaat 4680 gtatatcatt ttactggggg accttgtgca gaactcgtgg tgctgggcac tgctgctgct 4740 gcggcagctg gcaacctgac ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg 4800 agcccctgcg gacggtgccg acaggtgctt ctcgatctgc atcctgggat caaagccata 4860 gtgaaggaca gtgatggaca gccgacggca gttgggattc gtgaattgct gccctctggt 4920 tatgtgtggg agggctaagc acttcgtggc cgaggagcag gactgacacg tgctacgaga 4980 tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc 5040 cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt 5100 gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa 5160 agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca 5220 tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc 5280 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg 5340 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc 5400 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 5460 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 5520 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 5580 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccaggaac cgtaaaaagg 5640 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 5700 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 5760 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 5820 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 5880 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 5940 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 6000 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 6060 tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc 6120 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 6180 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 6240 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 6300 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 6360 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 6420 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 6480 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 6540 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 6600 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 6660 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 6720 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 6780 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 6840 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 6900 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 6960 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 7020 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 7080 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 7140 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 7200 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 7260 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 7320 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 7380 gcacatttcc ccgaaaagtg ccacctgacg tc 7412 //