LOCUS       pEF6-E-hPKR-KD          6723 bp    DNA     circular     29-OCT-2007
DEFINITION  LMBP 4511.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4511
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium).
            Last modified: 29-OCT-2007
COMMENT     VNTNAME|pEF6-E-hPKR-KD|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF6-E-hPKR-KD" 0 6723 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 0 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43) 
COMMENT        (CWordArray) (CStringList "NcoI" "NotI" "SalI" "XbaI" "XmaI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 6723 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "NcoI" "ccatgg" 2 0 3 1635 0 3630 0 3850 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 1680 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "SalI" "gtcgac" 2 0 2 4545 0 6722 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 2591 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 3744 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "XmnI" "gaannnnttc" 6 0 4 651 0 2376 0 3395 0 
COMMENT            6400 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "hEF1alpha promoter" 29 0 0 386 1529 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "386..1529") 
COMMENT        #7=(CFSignal (CObList) "intron" 15 0 0 582 1520 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "582..1520") 
COMMENT        #8=(CFSignal (CObList) "T7 promoter" 30 0 0 1546 1562 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1546..1562") 
COMMENT        #9=(CFSignal (CObList) "hPKR-KD" 4 0 0 1687 2589 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1687..2589") 
COMMENT        #10=(CFSignal (CObList) "Myc epitope" 22 0 0 2605 2634 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2605..2634") 
COMMENT        #11=(CFSignal (CObList) "His-tag" 22 0 0 2650 2667 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2650..2667") 
COMMENT        #12=(CFSignal (CObList) "bovine growth hormone polyA" 43 0 0 2696 2920 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "2696..2920") 
COMMENT        #13=(CFSignal (CObList) "f1 ori" 33 0 0 2966 3394 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2966..3394") 
COMMENT        #14=(CFSignal (CObList) "SV40 early promoter" 29 0 0 3399 3680 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3399..3680") 
COMMENT        #15=(CFSignal (CObList) "SV40 ori" 33 0 0 3637 3712 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3637..3712") 
COMMENT        #16=(CFSignal (CObList) "EM7 promoter" 30 0 0 3785 3850 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3785..3850") 
COMMENT        #17=(CFSignal (CObList) "blasticidin" 4 0 0 3851 4249 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3851..4249") 
COMMENT        #18=(CFSignal (CObList) "SV40 polyA early" 43 0 0 4407 4481 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4407..4481") 
COMMENT        #19=(CFSignal (CObList) "SV40 polyA late" 43 0 1 4407 4481 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4407..4481)") 
COMMENT        #20=(CFSignal (CObList) "pMB1 ori" 33 0 1 4795 5186 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4795..5186)") 
COMMENT        #21=(CFSignal (CObList) "ampicillin" 4 0 1 5727 6587 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5727..6587)") 
COMMENT        #22=(CFSignal (CObList) "E-tag" 22 0 0 1639 1677 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1639..1677")) (CObList) (CObList) (CObList) 
COMMENT       (CObList) 
COMMENT       (CObList 
COMMENT        #23=(CFSignal (CObList) "LMBP4511" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #24=(CFSignal (CObList) "Last modified: 29-OCT-2007" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT             (CObjectList 
COMMENT              #26=(CRefLinePar 
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COMMENT                   5 "" 0 4) 
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COMMENT                    (CObjectList 
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COMMENT                          "DNA Plasmid 'pEF6-E-hPKR-KD'" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4511." 
COMMENT                          1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 10/29/07 10:33" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6723 bp" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #36=(CFolderPar 
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COMMENT                    (CObjectList 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 23-JAN-2007" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #39=(CRefLinePar 
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COMMENT                   0 0) 
COMMENT              #40=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #41=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP4511") 
COMMENT                     #43=(CFolderPar 
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COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "Last modified: 29-OCT-2007"))) 
COMMENT                   "Annotations") 
COMMENT              #45=(CFolderPar 
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COMMENT                    (CObjectList 
COMMENT                     #46=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #47=(CFolderPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #48=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1687  End: 2589" 1) 
COMMENT                                   #49=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #50=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1687..2589" 1))) "hPKR-KD") 
COMMENT                            #51=(CFolderPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        "Start: 3851  End: 4249" 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #54=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3851..4249" 1))) "blasticidin") 
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COMMENT                                        "Start: 5727  End: 6587 (Complementary)" 
COMMENT                                        1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(5727..6587)" 1))) 
COMMENT                                 "ampicillin"))) "CDS (3 total)") 
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COMMENT                          "Intron (1 total)") 
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COMMENT                                        1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1639..1677" 1))) "E-tag") 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2605  End: 2634" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2605..2634" 1))) "Myc epitope") 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2650 (3 #11# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2650  End: 2667" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2650..2667" 1))) "His-tag"))) 
COMMENT                          "Misc. Marker (3 total)") 
COMMENT                     #78=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
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COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 386   End: 1529" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   386..1529" 1))) "hEF1alpha promoter") 
COMMENT                            #83=(CFolderPar 
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COMMENT                                  (CParagraph 3399 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #84=(CLinePar 
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COMMENT                                   #85=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3399..3680" 1))) 
COMMENT                                 "SV40 early promoter"))) 
COMMENT                          "Promoter Eukaryotic (2 total)") 
COMMENT                     #87=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                   #90=(CLinePar 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1546..1562" 1))) "T7 promoter") 
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COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3785..3850" 1))) "EM7 promoter"))) 
COMMENT                          "Promoter Prokaryotic (2 total)") 
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COMMENT                                   #100=(CLinePar 
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COMMENT                                          "   complement(4795..5186)" 1))) 
COMMENT                                  "pMB1 ori"))) "Replication Origin (3 total)") 
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COMMENT                                           "   2696..2920" 1))) 
COMMENT                                   "bovine growth hormone polyA") 
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COMMENT                                   "SV40 polyA early") 
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COMMENT                                     #120=(CLinePar 
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COMMENT                                           "   complement(4407..4481)" 1))) 
COMMENT                                   "SV40 polyA late"))) "Terminator (3 total)"))) 
COMMENT                   "Feature Map") 
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COMMENT                     (CObjectList 
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COMMENT                                            " N1: 1635 " 1) 
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COMMENT                                            " N2: 3630 " 1) 
COMMENT                                      #127=(CLinePar 
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COMMENT                                            " N3: 3850 " 1))))) "NcoI: 3 sites") 
COMMENT                            1 5 "CCATGG" "GGTACC") 
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COMMENT                                            " N1: 1680 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
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COMMENT                                            " N1: 4545 " 1) 
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COMMENT                                            " N2: 6722 " 1))))) "SalI: 2 sites") 
COMMENT                            1 5 "GTCGAC" "CAGCTG") 
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COMMENT                                            " N1: 2591 " 1))))) "XbaI: 1 site") 
COMMENT                            1 5 "TCTAGA" "AGATCT") 
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FEATURES             Location/Qualifiers
     promoter        386..1529
                     /vntifkey="29"
                     /label=hEF1alpha\promoter
                     /note="human elongation factor 1alpha promoter"
     intron          582..1520
                     /vntifkey="15"
                     /label=intron
                     /note="human elongation factor 1alpha intron"
     promoter        1546..1562
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="phage T7 gene 10 promoter"
     misc_marker     1639..1677
                     /vntifkey="22"
                     /label=E-tag
                     /note="E-tag"
     CDS             1687..2589
                     /vntifkey="4"
                     /label=hPKR-KD
                     /note="caspase-generated kinase domain of the hPKR coding sequence"
     misc_marker     2605..2634
                     /vntifkey="22"
                     /label=Myc\epitope
                     /note="Myc epitope"
     misc_marker     2650..2667
                     /vntifkey="22"
                     /label=His-tag
                     /note="polyhistidine (6 x His) tag"
     terminator      2696..2920
                     /vntifkey="43"
                     /label=bovine\growth\hormone\polyA
                     /note="polyA signal of the bovine growth hormone"
     rep_origin      2966..3394
                     /vntifkey="33"
                     /label=f1\ori
                     /note="phage f1 origin"
     promoter        3399..3680
                     /vntifkey="29"
                     /label=SV40\early\promoter
                     /note="SV40 early promoter"
     rep_origin      3637..3712
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     promoter        3785..3850
                     /vntifkey="30"
                     /label=EM7\promoter
                     /note="synthetic prokaryotic EM7 promoter"
     CDS             3851..4249
                     /vntifkey="4"
                     /label=blasticidin
                     /note="antibiotic resistance gene; coding sequence"
     terminator      4407..4481
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(4407..4481)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(4795..5186)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(5727..6587)
                     /vntifkey="4"
                     /label=ampicillin
                     /note="antibiotic resistance gene; coding sequence"
BASE COUNT     1634 a      1625 c      1751 g      1713 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 
      301 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 
      361 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 
      421 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 
      481 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 
      541 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 
      601 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 
      661 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 
      721 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 
      781 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 
      841 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 
      901 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 
      961 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 
     1021 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 
     1081 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 
     1141 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 
     1201 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 
     1261 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 
     1321 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 
     1381 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 
     1441 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 
     1501 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 
     1561 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 
     1621 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 
     1681 gccgctatga aagaaacaaa gtatactgtg gacaagaggt ttggcatgga ttttaaagaa 
     1741 atagaattaa ttggctcagg tggatttggc caagttttca aagcaaaaca cagaattgac 
     1801 ggaaagactt acgttattaa acgtgttaaa tataataacg agaaggcgga gcgtgaagta 
     1861 aaagcattgg caaaacttga tcatgtaaat attgttcact acaatggctg ttgggatgga 
     1921 tttgattatg atcctgagac cagtgatgat tctcttgaga gcagtgatta tgatcctgag 
     1981 aacagcaaaa atagttcaag gtcaaagact aagtgccttt tcatccaaat ggaattctgt 
     2041 gataaaggga ccttggaaca atggattgaa aaaagaagag gcgagaaact agacaaagtt 
     2101 ttggctttgg aactctttga acaaataaca aaaggggtgg attatataca ttcaaaaaaa 
     2161 ttaattcata gagatcttaa gccaagtaat atattcttag tagatacaaa acaagtaaag 
     2221 attggagact ttggacttgt aacatctctg aaaaatgatg gaaagcgaac aaggagtaag 
     2281 ggaactttgc gatacatgag cccagaacag atttcttcgc aagactatgg aaaggaagtg 
     2341 gacctctacg ctttggggct aattcttgct gaacttcttc atgtatgtga cactgctttt 
     2401 gaaacatcaa agtttttcac agacctacgg gatggcatca tctcagatat atttgataaa 
     2461 aaagaaaaaa ctcttctaca gaaattactc tcaaagaaac ctgaggatcg acctaacaca 
     2521 tctgaaatac taaggacctt gactgtgtgg aagaaaagcc cagagaaaaa tgaacgacac 
     2581 acatgttagt ctagagggcc cttcgaacaa aaactcatct cagaagagga tctgaatatg 
     2641 cataccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg 
     2701 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 
     2761 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 
     2821 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 
     2881 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 
     2941 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 
     3001 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 
     3061 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 
     3121 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 
     3181 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 
     3241 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 
     3301 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 
     3361 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 
     3421 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 
     3481 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 
     3541 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 
     3601 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     3661 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     3721 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc 
     3781 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag 
     3841 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac 
     3901 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc 
     3961 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc 
     4021 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt 
     4081 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct 
     4141 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc 
     4201 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg 
     4261 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa 
     4321 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 
     4381 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 
     4441 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 
     4501 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 
     4561 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 
     4621 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 
     4681 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 
     4741 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 
     4801 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 
     4861 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 
     4921 tgtgagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 
     4981 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 
     5041 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 
     5101 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 
     5161 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 
     5221 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 
     5281 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 
     5341 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 
     5401 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 
     5461 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 
     5521 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 
     5581 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 
     5641 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 
     5701 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 
     5761 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 
     5821 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 
     5881 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 
     5941 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 
     6001 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 
     6061 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 
     6121 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 
     6181 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 
     6241 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 
     6301 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 
     6361 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 
     6421 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 
     6481 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 
     6541 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 
     6601 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 
     6661 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 
     6721 gtc 
//
