ID pEF6-E-hPKR-KD standard; circular DNA; ; 6723 BP. DE LMBP 4511. CC Accession number: LMBP 4511 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium). CC Last modified: 29-OCT-2007 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 386..1529 FT /vntifkey="29" FT /label=hEF1alpha\promoter FT /note="human elongation factor 1alpha promoter" FT intron 582..1520 FT /vntifkey="15" FT /label=intron FT /note="human elongation factor 1alpha intron" FT promoter 1546..1562 FT /vntifkey="30" FT /label=T7\promoter FT /note="phage T7 gene 10 promoter" FT misc_marker 1639..1677 FT /vntifkey="22" FT /label=E-tag FT /note="E-tag" FT CDS 1687..2589 FT /vntifkey="4" FT /label=hPKR-KD FT /note="caspase-generated kinase domain of the hPKR coding sequence" FT misc_marker 2605..2634 FT /vntifkey="22" FT /label=Myc\epitope FT /note="Myc epitope" FT misc_marker 2650..2667 FT /vntifkey="22" FT /label=His-tag FT /note="polyhistidine (6 x His) tag" FT terminator 2696..2920 FT /vntifkey="43" FT /label=bovine\growth\hormone\polyA FT /note="polyA signal of the bovine growth hormone" FT rep_origin 2966..3394 FT /vntifkey="33" FT /label=f1\ori FT /note="phage f1 origin" FT promoter 3399..3680 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="SV40 early promoter" FT rep_origin 3637..3712 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT promoter 3785..3850 FT /vntifkey="30" FT /label=EM7\promoter FT /note="synthetic prokaryotic EM7 promoter" FT CDS 3851..4249 FT /vntifkey="4" FT /label=blasticidin FT /note="antibiotic resistance gene; coding sequence" FT terminator 4407..4481 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator complement(4407..4481) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT rep_origin complement(4795..5186) FT /vntifkey="33" FT /label=pMB1\ori FT /note="origin plasmid" FT CDS complement(5727..6587) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" SQ Sequence 6723 BP; 1634 A; 1625 C; 1751 G; 1713 t; gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 60 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 120 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 180 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 240 gattattgac taggcttttg caaaaagctt tgcaaagatg gataaagttt taaacagaga 300 ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg 360 cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg 420 gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt 480 actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg 540 tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt 600 cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct 660 ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga 720 ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct 780 ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt 840 ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc 900 ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg 960 acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac 1020 cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc 1080 cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag 1140 cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct 1200 cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg 1260 tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga 1320 gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc 1380 acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg 1440 aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa 1500 gtttttttct tccatttcag gtgtcgtgag gaattagctt ggtactaata cgactcacta 1560 tagggagacc caagctggct aggtaagctt ggtaccgagc tcggatccac tagtccagtg 1620 tggtggaatt ccaccatggg tgcgccggtg ccgtatccgg acccgctgga accgcgtgcg 1680 gccgctatga aagaaacaaa gtatactgtg gacaagaggt ttggcatgga ttttaaagaa 1740 atagaattaa ttggctcagg tggatttggc caagttttca aagcaaaaca cagaattgac 1800 ggaaagactt acgttattaa acgtgttaaa tataataacg agaaggcgga gcgtgaagta 1860 aaagcattgg caaaacttga tcatgtaaat attgttcact acaatggctg ttgggatgga 1920 tttgattatg atcctgagac cagtgatgat tctcttgaga gcagtgatta tgatcctgag 1980 aacagcaaaa atagttcaag gtcaaagact aagtgccttt tcatccaaat ggaattctgt 2040 gataaaggga ccttggaaca atggattgaa aaaagaagag gcgagaaact agacaaagtt 2100 ttggctttgg aactctttga acaaataaca aaaggggtgg attatataca ttcaaaaaaa 2160 ttaattcata gagatcttaa gccaagtaat atattcttag tagatacaaa acaagtaaag 2220 attggagact ttggacttgt aacatctctg aaaaatgatg gaaagcgaac aaggagtaag 2280 ggaactttgc gatacatgag cccagaacag atttcttcgc aagactatgg aaaggaagtg 2340 gacctctacg ctttggggct aattcttgct gaacttcttc atgtatgtga cactgctttt 2400 gaaacatcaa agtttttcac agacctacgg gatggcatca tctcagatat atttgataaa 2460 aaagaaaaaa ctcttctaca gaaattactc tcaaagaaac ctgaggatcg acctaacaca 2520 tctgaaatac taaggacctt gactgtgtgg aagaaaagcc cagagaaaaa tgaacgacac 2580 acatgttagt ctagagggcc cttcgaacaa aaactcatct cagaagagga tctgaatatg 2640 cataccggtc atcatcacca tcaccattga gtttaaaccc gctgatcagc ctcgactgtg 2700 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa 2760 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt 2820 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa 2880 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc 2940 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt 3000 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc 3060 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg 3120 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat 3180 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg 3240 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct 3300 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa 3360 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag 3420 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt 3480 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca 3540 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 3600 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 3660 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag 3720 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcagc 3780 acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg acaaggtgag 3840 gaactaaacc atggccaagc ctttgtctca agaagaatcc accctcattg aaagagcaac 3900 ggctacaatc aacagcatcc ccatctctga agactacagc gtcgccagcg cagctctctc 3960 tagcgacggc cgcatcttca ctggtgtcaa tgtatatcat tttactgggg gaccttgtgc 4020 agaactcgtg gtgctgggca ctgctgctgc tgcggcagct ggcaacctga cttgtatcgt 4080 cgcgatcgga aatgagaaca ggggcatctt gagcccctgc ggacggtgcc gacaggtgct 4140 tctcgatctg catcctggga tcaaagccat agtgaaggac agtgatggac agccgacggc 4200 agttgggatt cgtgaattgc tgccctctgg ttatgtgtgg gagggctaag cacttcgtgg 4260 ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc ttctatgaaa 4320 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc 4380 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat 4440 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 4500 gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga 4560 gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc 4620 cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct 4680 aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc 4740 agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt 4800 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 4860 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 4920 tgtgagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc 4980 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca 5040 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg 5100 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct 5160 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt 5220 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag 5280 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc 5340 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac 5400 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga 5460 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc 5520 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg 5580 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca 5640 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt 5700 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca 5760 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg 5820 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca 5880 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt 5940 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt 6000 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca 6060 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca 6120 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga 6180 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact 6240 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga 6300 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg 6360 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc 6420 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga 6480 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat 6540 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt 6600 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt 6660 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac 6720 gtc 6723 //