LOCUS       pDEST-Etag              6038 bp    DNA     circular      4-FEB-2011
DEFINITION  LMBP 4540.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4540
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 4-FEB-2011
            
COMMENT     VNTNAME|pDEST-Etag|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pDEST-Etag" 0 6038 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 0 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BstXI" "SacI") (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") (CObList) 1 "{(0,1),2}" 0 0 "" 0 
COMMENT        4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 82 178 1 6038 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #0=(CFSignal (CObList) "pMB1 ori" 33 0 1 526 928 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(526..928)") 
COMMENT        #1=(CFSignal (CObList) "ccdB" 4 0 0 5544 5849 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5544..5849") 
COMMENT        #2=(CFSignal (CObList) "f1 ori" 33 0 1 2460 2915 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2460..2915)") 
COMMENT        #3=(CFSignal (CObList) "T3 promoter" 30 0 1 271 287 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(271..287)") 
COMMENT        #4=(CFSignal (CObList) "SP6 promoter" 30 0 0 4008 4024 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "4008..4024") 
COMMENT        #5=(CFSignal (CObList) "T7 promoter" 30 0 1 1 17 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1..17)") 
COMMENT        #6=(CFSignal (CObList) "SV40 polyA early" 43 0 1 82 178 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(82..178)") 
COMMENT        #7=(CFSignal (CObList) "SV40 polyA late" 43 0 0 82 178 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "82..178") 
COMMENT        #8=(CFSignal (CObList) "sCMV-IE promoter and enhancer" 29 0 0 2989 4001 
COMMENT            0 (CStringList) (CStringList) 1 1 1 1 "2989..4001") 
COMMENT        #9=(CFSignal (CObList) "attR2" 0 0 0 5890 6014 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "5890..6014") 
COMMENT        #10=(CFSignal (CObList) "attR1" 0 0 1 4142 4266 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4142..4266)") 
COMMENT        #11=(CFSignal (CObList) "E-tag" 22 0 0 4073 4111 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "4073..4111") 
COMMENT        #12=(CFSignal (CObList) "lacUV5 promoter" 30 0 1 351 407 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "351..407") 
COMMENT        #13=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 4543 5202 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "4543..5202") 
COMMENT        #14=(CFSignal (CObList) "ampicillin resistance" 4 0 1 1469 2329 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(1469..2329)")) 
COMMENT       (CObList) (CObList) (CObList) (CObList) 
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COMMENT        #15=(CFSignal (CObList) "LMBP 4540" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #16=(CFSignal (CObList) "Last modified: 4-FEB-2011" 2 0 0 0 0 0 
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COMMENT                          "DNA Plasmid 'pDEST-Etag'" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 4540." 
COMMENT                          1) 
COMMENT                     #22=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #23=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #24=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 02/04/11 11:00" 1) 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6038 bp" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 30-MAR-2007" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                                 "" 1))) "LMBP 4540") 
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COMMENT                                   #41=(CLinePar 
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COMMENT                                        "Bacteriophage lambda attR1 recombination site" 
COMMENT                                        1) 
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COMMENT                                        "Original Location Description:" 1) 
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COMMENT                                        "Start: 5890  End: 6014" 1) 
COMMENT                                   #46=(CLinePar 
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COMMENT                                        "Bacteriophage lambda attR2 recombination site" 
COMMENT                                        1) 
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COMMENT                          "Attenuator (2 total)") 
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COMMENT                                        "B gene of control of cell death locus of  E. coli F plasmid (ccdB, lethal gene)" 
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COMMENT                                        "   5544..5849" 1))) "ccdB"))) 
COMMENT                          "CDS (3 total)") 
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COMMENT                                        "Start: 4073  End: 4111" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "N-terminal E-tag" 1) 
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COMMENT                                        "   4073..4111" 1))) "E-tag"))) 
COMMENT                          "Misc. Marker (1 total)") 
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COMMENT                                        "Start: 2989  End: 4001" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Simian cytomegalovirus immediate early promoter (CMV-IE) and enhancer" 
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COMMENT                                   #73=(CLinePar 
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COMMENT                                   #74=(CLinePar 
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COMMENT                                 "sCMV-IE promoter and enhancer"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
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COMMENT                                        "Start: 1     End: 17   (Complementary)" 
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COMMENT                                   #78=(CLinePar 
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COMMENT                                        "Phage T7 gene 10 promoter (T7g10)" 1) 
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COMMENT                                        "Start: 271   End: 287  (Complementary)" 
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COMMENT                                        "Phage T3 promoter" 1) 
COMMENT                                   #84=(CLinePar 
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COMMENT                                   #85=(CLinePar 
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COMMENT                                        "   complement(271..287)" 1))) 
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COMMENT                                        "Escherichia coli lac operon promoter; mutant (lacUV5)" 
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COMMENT                                        "Phage SP6 promoter" 1) 
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COMMENT                          "Promoter Prokaryotic (4 total)") 
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COMMENT                                        "Escherichia coli plasmid pMB1 origin" 1) 
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COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
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COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
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COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
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COMMENT                                   "@N" 1 -14.8496 -7.14286 0 -10 5.72136 
COMMENT                                   1.11636 #277#) 
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FEATURES             Location/Qualifiers
     rep_origin      complement(526..928)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="Escherichia coli plasmid pMB1 origin"
     CDS             complement(1469..2329)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             4543..5202
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             5544..5849
                     /vntifkey="4"
                     /label=ccdB
                     /note="B gene of control of cell death locus of  E. coli F plasmid (ccdB, lethal gene)"
     rep_origin      complement(2460..2915)
                     /vntifkey="33"
                     /label=f1\ori
                     /note="Phage f1 origin"
     promoter        complement(271..287)
                     /vntifkey="30"
                     /label=T3\promoter
                     /note="Phage T3 promoter"
     promoter        4008..4024
                     /vntifkey="30"
                     /label=SP6\promoter
                     /note="Phage SP6 promoter"
     promoter        complement(1..17)
                     /vntifkey="30"
                     /label=T7\promoter
                     /note="Phage T7 gene 10 promoter (T7g10)"
     terminator      complement(82..178)
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      82..178
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     promoter        2989..4001
                     /vntifkey="29"
                     /label=sCMV-IE\promoter\and\enhancer
                     /note="Simian cytomegalovirus immediate early promoter (CMV-IE) and enhancer"
     attenuator      5890..6014
                     /vntifkey="0"
                     /label=attR2
                     /note="Bacteriophage lambda attR2 recombination site"
     attenuator      complement(4142..4266)
                     /vntifkey="0"
                     /label=attR1
                     /note="Bacteriophage lambda attR1 recombination site"
     misc_marker     4073..4111
                     /vntifkey="22"
                     /label=E-tag
                     /note="N-terminal E-tag"
     promoter        complement(351..407)
                     /vntifkey="30"
                     /label=lacUV5\promoter
                     /note="Escherichia coli lac operon promoter; mutant (lacUV5)"
BASE COUNT     1589 a      1407 c      1470 g      1572 t 
ORIGIN
        1 tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa 
       61 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 
      121 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 
      181 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg 
      241 cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt 
      301 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 
      361 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 
      421 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 
      481 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 
      541 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 
      601 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 
      661 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 
      721 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 
      781 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 
      841 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 
      901 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 
      961 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 
     1021 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 
     1081 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 
     1141 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 
     1201 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 
     1261 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 
     1321 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 
     1381 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 
     1441 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 
     1501 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 
     1561 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 
     1621 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 
     1681 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 
     1741 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 
     1801 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 
     1861 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 
     1921 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 
     1981 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 
     2041 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 
     2101 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 
     2161 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 
     2221 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 
     2281 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 
     2341 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 
     2401 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta 
     2461 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 
     2521 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 
     2581 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 
     2641 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 
     2701 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 
     2761 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 
     2821 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 
     2881 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa 
     2941 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga ccatagccaa 
     3001 ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa 
     3061 ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa 
     3121 ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacttggc actgtgccaa 
     3181 ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca 
     3241 agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag 
     3301 gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc 
     3361 aatactatgt attggcccta tgccatatag tattccatat atgggttttc ctattgacgt 
     3421 agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc 
     3481 atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat 
     3541 atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg 
     3601 cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga 
     3661 tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat 
     3721 ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg 
     3781 gaaggacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg 
     3841 cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg 
     3901 cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc 
     3961 tcgtttaggg aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac 
     4021 tatagaatac aagctacttg ttctttttgc aggatcccat cgatccccca tgggtgcgcc 
     4081 ggtgccgtat ccggacccgc tggaaccgcg tgcggcccat cgattcgaat tcaaggatca 
     4141 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 
     4201 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 
     4261 actatggcgg ccgcgggtga tgctgccaac ttagcggccg ctaagttggc agcatcaccc 
     4321 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat 
     4381 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt 
     4441 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta 
     4501 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact 
     4561 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag 
     4621 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag 
     4681 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg 
     4741 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat 
     4801 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg 
     4861 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt 
     4921 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca 
     4981 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc 
     5041 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg 
     5101 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat 
     5161 gaattacaac agtactgcga tgagtggcag ggcggggcgt aatctagagg atccggctta 
     5221 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat 
     5281 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag 
     5341 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg 
     5401 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc 
     5461 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc 
     5521 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc 
     5581 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg 
     5641 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg 
     5701 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg 
     5761 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg 
     5821 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg 
     5881 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta 
     5941 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc 
     6001 ttgtacaaag tggttcgatc ctctcgagcc tctagaac 
//
