ID pDEST-Etag standard; circular DNA; ; 6038 BP. DE LMBP 4540. CC Accession number: LMBP 4540 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) FH Key Location/Qualifiers FH FT rep_origin complement(526..928) FT /vntifkey="33" FT /label=pMB1\ori FT /note="Escherichia coli plasmid pMB1 origin" FT CDS complement(1469..2329) FT /vntifkey="4" FT /label=ampicillin FT /note="antibiotic resistance gene; coding sequence" FT CDS 4543..5202 FT /vntifkey="4" FT /label=chloramphenicol FT /note="antibiotic resistance gene; coding sequence" FT CDS 5544..5849 FT /vntifkey="4" FT /label=ccdB FT /note="B gene of control of cell death locus of E. coli F plasmid (ccdB, lethal gene)" FT rep_origin complement(2460..2915) FT /vntifkey="33" FT /label=f1\ori FT /note="Phage f1 origin" FT promoter complement(271..287) FT /vntifkey="30" FT /label=T3\promoter FT /note="Phage T3 promoter" FT promoter 4008..4024 FT /vntifkey="30" FT /label=SP6\promoter FT /note="Phage SP6 promoter" FT promoter complement(1..17) FT /vntifkey="30" FT /label=T7\promoter FT /note="Phage T7 gene 10 promoter (T7g10)" FT terminator complement(82..178) FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT terminator 82..178 FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT promoter 2989..4001 FT /vntifkey="29" FT /label=sCMV-IE\promoter\and\enhancer FT /note="Simian cytomegalovirus immediate early promoter (CMV-IE) and enhancer" FT attenuator 5890..6014 FT /vntifkey="0" FT /label=attR2 FT /note="Bacteriophage lambda attR2 recombination site" FT attenuator complement(4142..4266) FT /vntifkey="0" FT /label=attR1 FT /note="Bacteriophage lambda attR1 recombination site" FT misc_marker 4073..4111 FT /vntifkey="22" FT /label=E-tag FT /note="N-terminal E-tag" FT promoter 351..407 FT /vntifkey="30" FT /label=lacUV5\promoter FT /note="Escherichia coli lac operon promoter; mutant (lacUV5)" SQ Sequence 6038 BP; 1589 A; 1407 C; 1470 G; 1572 t; tatagtgagt cgtattacgt agatccagac atgataagat acattgatga gtttggacaa 60 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct 120 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt 180 atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc ggcgccaatg 240 cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg cgcttggcgt 300 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca 360 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat 420 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 480 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct 540 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa 600 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa 660 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 720 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 780 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 840 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 900 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 960 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 1020 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 1080 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 1140 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 1200 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 1260 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 1320 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat 1380 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa 1440 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct 1500 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta 1560 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct 1620 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg 1680 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa 1740 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt 1800 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta 1860 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca 1920 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta 1980 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct 2040 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg 2100 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac 2160 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact 2220 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa 2280 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt 2340 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat 2400 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta 2460 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt 2520 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat 2580 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa 2640 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta 2700 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc 2760 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc 2820 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac 2880 acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa 2940 ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagtcga ccatagccaa 3000 ttcaatatgg cgtatatgga ctcatgccaa ttcaatatgg tggatctgga cctgtgccaa 3060 ttcaatatgg cgtatatgga ctcgtgccaa ttcaatatgg tggatctgga ccccagccaa 3120 ttcaatatgg cggacttggc accatgccaa ttcaatatgg cggacttggc actgtgccaa 3180 ctggggaggg gtctacttgg cacggtgcca agtttgagga ggggtcttgg ccctgtgcca 3240 agtccgccat attgaattgg catggtgcca ataatggcgg ccatattggc tatatgccag 3300 gatcaatata taggcaatat ccaatatggc cctatgccaa tatggctatt ggccaggttc 3360 aatactatgt attggcccta tgccatatag tattccatat atgggttttc ctattgacgt 3420 agatagcccc tcccaatggg cggtcccata taccatatat ggggcttcct aataccgccc 3480 atagccactc ccccattgac gtcaatggtc tctatatatg gtctttccta ttgacgtcat 3540 atgggcggtc ctattgacgt atatggcgcc tcccccattg acgtcaatta cggtaaatgg 3600 cccgcctggc tcaatgccca ttgacgtcaa taggaccacc caccattgac gtcaatggga 3660 tggctcattg cccattcata tccgttctca cgccccctat tgacgtcaat gacggtaaat 3720 ggcccacttg gcagtacatc aatatctatt aatagtaact tggcaagtac attactattg 3780 gaaggacgcc agggtacatt ggcagtactc ccattgacgt caatggcggt aaatggcccg 3840 cgatggctgc caagtacatc cccattgacg tcaatgggga ggggcaatga cgcaaatggg 3900 cgttccattg acgtaaatgg gcggtaggcg tgcctaatgg gaggtctata taagcaatgc 3960 tcgtttaggg aaccgccatt ctgcctgggg acgtcggagc aagcttgatt taggtgacac 4020 tatagaatac aagctacttg ttctttttgc aggatcccat cgatccccca tgggtgcgcc 4080 ggtgccgtat ccggacccgc tggaaccgcg tgcggcccat cgattcgaat tcaaggatca 4140 aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat gatataaata tcaatatatt 4200 aaattagatt ttgcataaaa aacagactac ataatactgt aaaacacaac atatccagtc 4260 actatggcgg ccgcgggtga tgctgccaac ttagcggccg ctaagttggc agcatcaccc 4320 gacgcacttt gcgccgaata aatacctgtg acggaagatc acttcgcaga ataaataaat 4380 cctggtgtcc ctgttgatac cgggaagccc tgggccaact tttggcgaaa atgagacgtt 4440 gatcggcacg taagaggttc caactttcac cataatgaaa taagatcact accgggcgta 4500 ttttttgagt tatcgagatt ttcaggagct aaggaagcta aaatggagaa aaaaatcact 4560 ggatatacca ccgttgatat atcccaatgg catcgtaaag aacattttga ggcatttcag 4620 tcagttgctc aatgtaccta taaccagacc gttcagctgg atattacggc ctttttaaag 4680 accgtaaaga aaaataagca caagttttat ccggccttta ttcacattct tgcccgcctg 4740 atgaatgctc atccggaatt ccgtatggca atgaaagacg gtgagctggt gatatgggat 4800 agtgttcacc cttgttacac cgttttccat gagcaaactg aaacgttttc atcgctctgg 4860 agtgaatacc acgacgattt ccggcagttt ctacacatat attcgcaaga tgtggcgtgt 4920 tacggtgaaa acctggccta tttccctaaa gggtttattg agaatatgtt tttcgtctca 4980 gccaatccct gggtgagttt caccagtttt gatttaaacg tggccaatat ggacaacttc 5040 ttcgcccccg ttttcaccat gggcaaatat tatacgcaag gcgacaaggt gctgatgccg 5100 ctggcgattc aggttcatca tgccgtctgt gatggcttcc atgtcggcag aatgcttaat 5160 gaattacaac agtactgcga tgagtggcag ggcggggcgt aatctagagg atccggctta 5220 ctaaaagcca gataacagta tgcgtatttg cgcgctgatt tttgcggtat aagaatatat 5280 actgatatgt atacccgaag tatgtcaaaa agaggtgtgc tatgaagcag cgtattacag 5340 tgacagttga cagcgacagc tatcagttgc tcaaggcata tatgatgtca atatctccgg 5400 tctggtaagc acaaccatgc agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc 5460 ggaaaatcag gaagggatgg ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc 5520 tgacgagaac agggactggt gaaatgcagt ttaaggttta cacctataaa agagagagcc 5580 gttatcgtct gtttgtggat gtacagagtg atattattga cacgcccggg cgacggatgg 5640 tgatccccct ggccagtgca cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg 5700 tggtgcatat cggggatgaa agctggcgca tgatgaccac cgatatggcc agtgtgccgg 5760 tctccgttat cggggaagaa gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg 5820 ccattaacct gatgttctgg ggaatataaa tgtcaggctc ccttatacac agccagtctg 5880 caggtcgacc atagtgactg gatatgttgt gttttacagt attatgtagt ctgtttttta 5940 tgcaaaatct aatttaatat attgatattt atatcatttt acgtttctcg ttcagctttc 6000 ttgtacaaag tggttcgatc ctctcgagcc tctagaac 6038 //