LOCUS       pDEST-GBKT7             8993 bp    DNA     circular      8-MAR-2011
DEFINITION  LMBP 4543.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4543
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 8-MAR-2011
            
            
COMMENT     VNTNAME|pDEST-GBKT7|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pDEST-GBKT7" 0 8993 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BanII" "BstZI" "EcoRI" "SspI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 8993 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList 
COMMENT        #0=(CRSite (CStringList) "BanII" "grgcyc" 6 0 4 4505 0 5216 0 8228 0 
COMMENT            8797 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "BstZI" "cggccg" 2 0 2 1435 0 5667 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 1755 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "SspI" "aatatt" 4 0 5 704 0 2067 0 5723 0 6715 
COMMENT            0 8514 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "GALbd" 4 0 0 762 1202 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "762..1202") 
COMMENT        #5=(CFSignal (CObList) "T7 promoter" 30 0 0 1213 1229 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "1213..1229") 
COMMENT        #6=(CFSignal (CObList) "ADH1 promoter" 29 0 0 336 739 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "336..739") 
COMMENT        #7=(CFSignal (CObList) "attR1" 0 0 1 1308 1432 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(1308..1432)") 
COMMENT        #8=(CFSignal (CObList) "attR2" 0 0 0 2888 3012 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "2888..3012") 
COMMENT        #9=(CFSignal (CObList) "chloramphenicol resistance" 4 0 0 1541 2200 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "1541..2200") 
COMMENT        #10=(CFSignal (CObList) "ccdB" 4 0 0 2542 2847 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "2542..2847") 
COMMENT        #11=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 1 4911 5705 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(4911..5705)") 
COMMENT        #12=(CFSignal (CObList) "TRP1" 4 0 0 7720 8394 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7720..8394") 
COMMENT        #13=(CFSignal (CObList) "f1 ori" 33 0 1 8497 8952 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(8497..8952)") 
COMMENT        #14=(CFSignal (CObList) "2mu ori" 33 0 1 5837 6817 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(5837..6817)") 
COMMENT        #15=(CFSignal (CObList) "T7 terminator" 43 0 0 3027 3074 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "3027..3074") 
COMMENT        #16=(CFSignal (CObList) "HSV-TK polyA" 43 0 1 4650 4719 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(4650..4719)") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 1 3530 3932 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "complement(3530..3932)") 
COMMENT        #18=(CFSignal (CObList) "ADH1 terminator" 43 0 0 3101 3202 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3101..3202") 
COMMENT        #19=(CFSignal (CObList) "Myc epitope" 22 0 0 1251 1280 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "1251..1280") 
COMMENT        #20=(CFSignal (CObList) "ampicillin promoter" 30 0 1 5741 5836 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(5741..5836)") 
COMMENT        #21=(CFSignal (CObList) "FLP terminator" 43 0 1 6895 6999 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "complement(6895..6999)") 
COMMENT        #22=(CFSignal (CObList) "lac promoter" 30 0 1 3355 3438 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "5' UTR of amp, incl. RBS" 32 0 1 5706 5740 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "lacUV5 promoter" 30 0 0 1436 1492 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "")) (CObList) (CObList) 
COMMENT       (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #25=(CFSignal (CObList) "Last modified: 8-MAR-2011" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "") 
COMMENT        #26=(CFSignal (CObList) "LMBP 4543" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #27=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #28=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "pDEST-GBKT7" 2) 5 
COMMENT                   "" 0 4) 
COMMENT              #29=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'pDEST-GBKT7'" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DMB 4543 (B. Janssens)." 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 1) 
COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Creation Date: ? (no data)" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 03/08/11 09:57" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 8993 bp" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #38=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 23-FEB-2011" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #41=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #42=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 2 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #43=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #44=(CLinePar (CParagraph 0 (15 #25# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 8-MAR-2011") 
COMMENT                     #45=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
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COMMENT                                 "" 1))) "LMBP 4543"))) "Annotations") 
COMMENT              #47=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #48=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 0 (7 0 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
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COMMENT                                        "Start: 1308  End: 1432 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #51=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #52=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(1308..1432)" 1))) "attR1") 
COMMENT                            #53=(CFolderPar 
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COMMENT                                  (CParagraph 2888 (3 #8# 0) 1 2 2 0 194) 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2888  End: 3012" 1) 
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COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #56=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2888..3012" 1))) "attR2"))) 
COMMENT                          "Attenuator (2 total)") 
COMMENT                     #57=(CFolderPar 
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COMMENT                                        "Start: 762   End: 1202" 1) 
COMMENT                                   #60=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   762..1202" 1))) "GALbd") 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1541  End: 2200" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1541..2200" 1))) 
COMMENT                                 "chloramphenicol resistance") 
COMMENT                            #66=(CFolderPar 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2542  End: 2847" 1) 
COMMENT                                   #68=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2542..2847" 1))) "ccdB") 
COMMENT                            #70=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4911 (3 #11# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4911  End: 5705 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   complement(4911..5705)" 1))) 
COMMENT                                 "Tn5 neomycin resistance") 
COMMENT                            #74=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7720 (3 #12# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7720  End: 8394" 1) 
COMMENT                                   #76=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7720..8394" 1))) "TRP1"))) 
COMMENT                          "CDS (5 total)") 
COMMENT                     #78=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 22 (7 22 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #79=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1251 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1251  End: 1280" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1251..1280" 1))) "Myc epitope"))) 
COMMENT                          "Misc. Marker (1 total)") 
COMMENT                     #83=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 336 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 336   End: 739 " 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   336..739" 1))) "ADH1 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #88=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #89=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1213 (3 #5# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1213  End: 1229" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   1213..1229" 1))) "T7 promoter") 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1436 (3 #24# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1436  End: 1492" 1))) 
COMMENT                                 "lacUV5 promoter") 
COMMENT                            #95=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3355 (3 #22# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3355  End: 3438 (Complementary)" 
COMMENT                                        1))) "lac promoter") 
COMMENT                            #97=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5741 (3 #20# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
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COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5741  End: 5836 (Complementary)" 
COMMENT                                        1) 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   complement(5741..5836)" 1))) 
COMMENT                                 "ampicillin promoter"))) 
COMMENT                          "Promoter Prokaryotic (4 total)") 
COMMENT                     #101=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5706  End: 5740 (Complementary)" 
COMMENT                                           1))) "5' UTR of amp, incl. RBS"))) 
COMMENT                           "RBS (1 total)") 
COMMENT                     #104=(CFolderPar 
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COMMENT                            (CObjectList 
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COMMENT                                   (CGroupPar 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 3530  End: 3932 (Complementary)" 
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COMMENT                                     #107=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(3530..3932)" 1))) 
COMMENT                                   "pMB1 ori") 
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COMMENT                                   (CGroupPar 
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COMMENT                                    (CObjectList 
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COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
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COMMENT                                     #111=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(5837..6817)" 1))) 
COMMENT                                   "2mu ori") 
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COMMENT                                    (CObjectList 
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COMMENT                                           1) 
COMMENT                                     #115=(CLinePar 
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COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(8497..8952)" 1))) 
COMMENT                                   "f1 ori"))) "Replication Origin (3 total)") 
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COMMENT                            (CObjectList 
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COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3027..3074" 1))) "T7 terminator") 
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COMMENT                                    (CObjectList 
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COMMENT                                     #124=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #125=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   3101..3202" 1))) 
COMMENT                                   "ADH1 terminator") 
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COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 4650 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 4650  End: 4719 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #129=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(4650..4719)" 1))) 
COMMENT                                   "HSV-TK polyA") 
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COMMENT                                    (CObjectList 
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COMMENT                                           "Start: 6895  End: 6999 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #132=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #133=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(6895..6999)" 1))) 
COMMENT                                   "FLP terminator"))) "Terminator (4 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #134=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #135=(CRSFolderPar 
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COMMENT                                     (CObjectList 
COMMENT                                      #137=(CLinePar 
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FEATURES             Location/Qualifiers
     CDS             762..1202
                     /vntifkey="4"
                     /label=GALbd
     promoter        1213..1229
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        336..739
                     /vntifkey="29"
                     /label=ADH1\promoter
     attenuator      complement(1308..1432)
                     /vntifkey="0"
                     /label=attR1
     attenuator      2888..3012
                     /vntifkey="0"
                     /label=attR2
     CDS             1541..2200
                     /vntifkey="4"
                     /label=chloramphenicol\resistance
     CDS             2542..2847
                     /vntifkey="4"
                     /label=ccdB
     CDS             complement(4911..5705)
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     CDS             7720..8394
                     /vntifkey="4"
                     /label=TRP1
     rep_origin      complement(8497..8952)
                     /vntifkey="33"
                     /label=f1\ori
     rep_origin      complement(5837..6817)
                     /vntifkey="33"
                     /label=2mu\ori
     terminator      3027..3074
                     /vntifkey="43"
                     /label=T7\terminator
     terminator      complement(4650..4719)
                     /vntifkey="43"
                     /label=HSV-TK\polyA
     rep_origin      complement(3530..3932)
                     /vntifkey="33"
                     /label=pMB1\ori
     terminator      3101..3202
                     /vntifkey="43"
                     /label=ADH1\terminator
     misc_marker     1251..1280
                     /vntifkey="22"
                     /label=Myc\epitope
     promoter        complement(5741..5836)
                     /vntifkey="30"
                     /label=ampicillin\promoter
     terminator      complement(6895..6999)
                     /vntifkey="43"
                     /label=FLP\terminator
     promoter        complement(3355..3438)
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             complement(5706..5740)
                     /vntifkey="32"
                     /label=5'\UTR\of\amp,\incl.\RBS
     promoter        1436..1492
                     /vntifkey="30"
                     /label=lacUV5\promoter
BASE COUNT     2457 a      1988 c      2134 g      2414 t 
ORIGIN
        1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg 
       61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg 
      121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 
      181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 
      241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 
      301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 
      361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga 
      421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 
      481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 
      541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt 
      601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 
      661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 
      721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 
      781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 
      841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 
      901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 
      961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 
     1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 
     1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 
     1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 
     1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc 
     1261 tgatctcaga ggaggacctg catatggcca tggaggccga attatcaaca agtttgtaca 
     1321 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc 
     1381 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc 
     1441 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag 
     1501 ttaggatccg gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg 
     1561 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc 
     1621 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac 
     1681 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat 
     1741 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag 
     1801 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag 
     1861 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta 
     1921 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc 
     1981 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt 
     2041 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct 
     2101 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga 
     2161 attacaacag tactgcgatg agtggcaggg cggggcgtaa agatctggat ccggcttact 
     2221 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac 
     2281 tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg 
     2341 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc 
     2401 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg 
     2461 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg 
     2521 acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag agagagccgt 
     2581 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg 
     2641 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg 
     2701 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc 
     2761 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc 
     2821 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca 
     2881 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg 
     2941 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt 
     3001 gtacaaagtg gttgatggcc gcataactag cataacccct tggggcctct aaacgggtct 
     3061 tgaggggttt tttgcgcgct tgcagccaag ctaattccgg gcgaatttct tatgatttat 
     3121 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc 
     3181 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct 
     3241 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg 
     3301 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 
     3361 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc 
     3421 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 
     3481 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
     3541 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
     3601 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 
     3661 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 
     3721 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 
     3781 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 
     3841 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 
     3901 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 
     3961 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 
     4021 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 
     4081 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 
     4141 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 
     4201 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 
     4261 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 
     4321 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 
     4381 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 
     4441 taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg 
     4501 agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg 
     4561 tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc 
     4621 ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc 
     4681 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg 
     4741 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg 
     4801 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat 
     4861 ggcaggttgg gcgtcgcttg gtcggttcat ttcgaacccc agagtcccgc tcagaagaac 
     4921 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc 
     4981 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac 
     5041 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag 
     5101 cggccatttt ccaccatgat attcggcaag caggcatcgt catgggtcac gacgagatcc 
     5161 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga 
     5221 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc 
     5281 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc 
     5341 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg 
     5401 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg 
     5461 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg 
     5521 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc 
     5581 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca 
     5641 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca 
     5701 atcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 
     5761 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 
     5821 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc 
     5881 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa 
     5941 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc 
     6001 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa 
     6061 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa 
     6121 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt 
     6181 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag 
     6241 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg 
     6301 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat 
     6361 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg 
     6421 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt 
     6481 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac 
     6541 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt 
     6601 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag 
     6661 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta 
     6721 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt 
     6781 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag 
     6841 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat 
     6901 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga 
     6961 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta 
     7021 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat 
     7081 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt 
     7141 ctcatccttc aatgctatca tttcctttga tattggatca tattaagaaa ccattattat 
     7201 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 
     7261 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 
     7321 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 
     7381 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac 
     7441 gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt 
     7501 actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct 
     7561 tgtcacctta cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat 
     7621 tcggtcgaaa aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg 
     7681 tgagtatacg tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca 
     7741 ggtagttctg gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt 
     7801 gctctagatt ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca 
     7861 attgacccgg ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca 
     7921 ggcactccga aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct 
     7981 ctggtcaatg attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa 
     8041 taccaagagt tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac 
     8101 atactactca gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa 
     8161 gcaggtggga caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa 
     8221 gagagccccg aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt 
     8281 gatgcgctta gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat 
     8341 ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt 
     8401 actgagtagt atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata 
     8461 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt 
     8521 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 
     8581 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 
     8641 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 
     8701 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 
     8761 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 
     8821 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 
     8881 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 
     8941 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gat 
//
