ID pDEST-GBKT7 standard; circular DNA; ; 8993 BP. DE LMBP 4543. CC Accession number: LMBP 4543 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 8-MAR-2011 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT CDS 762..1202 FT /vntifkey="4" FT /label=GALbd FT promoter 1213..1229 FT /vntifkey="30" FT /label=T7\promoter FT promoter 336..739 FT /vntifkey="29" FT /label=ADH1\promoter FT attenuator complement(1308..1432) FT /vntifkey="0" FT /label=attR1 FT attenuator 2888..3012 FT /vntifkey="0" FT /label=attR2 FT CDS 1541..2200 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT CDS 2542..2847 FT /vntifkey="4" FT /label=ccdB FT CDS complement(4911..5705) FT /vntifkey="4" FT /label=Tn5\neomycin\resistance FT CDS 7720..8394 FT /vntifkey="4" FT /label=TRP1 FT rep_origin complement(8497..8952) FT /vntifkey="33" FT /label=f1\ori FT rep_origin complement(5837..6817) FT /vntifkey="33" FT /label=2mu\ori FT terminator 3027..3074 FT /vntifkey="43" FT /label=T7\terminator FT terminator complement(4650..4719) FT /vntifkey="43" FT /label=HSV-TK\polyA FT rep_origin complement(3530..3932) FT /vntifkey="33" FT /label=pMB1\ori FT terminator 3101..3202 FT /vntifkey="43" FT /label=ADH1\terminator FT misc_marker 1251..1280 FT /vntifkey="22" FT /label=Myc\epitope FT promoter complement(5741..5836) FT /vntifkey="30" FT /label=ampicillin\promoter FT terminator complement(6895..6999) FT /vntifkey="43" FT /label=FLP\terminator FT promoter complement(3355..3438) FT /vntifkey="30" FT /label=lac\promoter FT RBS complement(5706..5740) FT /vntifkey="32" FT /label=5'\UTR\of\amp,\incl.\RBS FT promoter 1436..1492 FT /vntifkey="30" FT /label=lacUV5\promoter SQ Sequence 8993 BP; 2457 A; 1988 C; 2134 G; 2414 t; cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg 60 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg 120 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 180 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 240 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 300 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 360 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga 420 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 480 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 540 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt 600 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 660 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 720 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 780 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 840 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 900 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 960 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 1020 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 1080 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 1140 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 1200 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc 1260 tgatctcaga ggaggacctg catatggcca tggaggccga attatcaaca agtttgtaca 1320 aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc 1380 ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc 1440 attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag 1500 ttaggatccg gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg 1560 atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc 1620 agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac 1680 cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat 1740 gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag 1800 tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag 1860 tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta 1920 cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc 1980 caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt 2040 cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct 2100 ggcgattcag gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga 2160 attacaacag tactgcgatg agtggcaggg cggggcgtaa agatctggat ccggcttact 2220 aaaagccaga taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac 2280 tgatatgtat acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg 2340 acagttgaca gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc 2400 tggtaagcac aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg 2460 aaaatcagga agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg 2520 acgagaacag ggactggtga aatgcagttt aaggtttaca cctataaaag agagagccgt 2580 tatcgtctgt ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg 2640 atccccctgg ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg 2700 gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc 2760 tccgttatcg gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc 2820 attaacctga tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca 2880 ggtcgaccat agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg 2940 caaaatctaa tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt 3000 gtacaaagtg gttgatggcc gcataactag cataacccct tggggcctct aaacgggtct 3060 tgaggggttt tttgcgcgct tgcagccaag ctaattccgg gcgaatttct tatgatttat 3120 gatttttatt attaaataag ttataaaaaa aataagtgta tacaaatttt aaagtgactc 3180 ttaggtttta aaacgaaaat tcttattctt gagtaactct ttcctgtagg tcaggttgct 3240 ttctcaggta tagcatgagg tcgctcttat tgaccacacc tctaccggca tgcaagcttg 3300 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 3360 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gaggtaactc 3420 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 3480 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 3540 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 3600 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 3660 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 3720 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 3780 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 3840 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 3900 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 3960 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 4020 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 4080 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 4140 ggctacacta gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 4200 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 4260 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 4320 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 4380 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 4440 taaagtatat atgagtaacc tgaggctatg gcagggcctg ccgccccgac gttggctgcg 4500 agccctgggc cttcacccga acttgggggg tggggtgggg aaaaggaaga aacgcgggcg 4560 tattggcccc aatggggtct cggtggggta tcgacagagt gccagccctg ggaccgaacc 4620 ccgcgtttat gaacaaacga cccaacaccg tgcgttttat tctgtctttt tattgccgtc 4680 atagcgcggg ttccttccgg tattgtctcc ttccgtgttt cagttagcct ccccctaggg 4740 tgggcgaaga actccagcat gagatccccg cgctggagga tcatccagcc ggcgtcccgg 4800 aaaacgattc cgaagcccaa cctttcatag aaggcggcgg tggaatcgaa atctcgtgat 4860 ggcaggttgg gcgtcgcttg gtcggttcat ttcgaacccc agagtcccgc tcagaagaac 4920 tcgtcaagaa ggcgatagaa ggcgatgcgc tgcgaatcgg gagcggcgat accgtaaagc 4980 acgaggaagc ggtcagccca ttcgccgcca agctcttcag caatatcacg ggtagccaac 5040 gctatgtcct gatagcggtc cgccacaccc agccggccac agtcgatgaa tccagaaaag 5100 cggccatttt ccaccatgat attcggcaag caggcatcgt catgggtcac gacgagatcc 5160 tcgccgtcgg gcatgctcgc cttgagcctg gcgaacagtt cggctggcgc gagcccctga 5220 tgctcttcgt ccagatcatc ctgatcgaca agaccggctt ccatccgagt acgtgctcgc 5280 tcgatgcgat gtttcgcttg gtggtcgaat gggcaggtag ccggatcaag cgtatgcagc 5340 cgccgcattg catcagccat gatggatact ttctcggcag gagcaaggtg agatgacagg 5400 agatcctgcc ccggcacttc gcccaatagc agccagtccc ttcccgcttc agtgacaacg 5460 tcgagcacag ctgcgcaagg aacgcccgtc gtggccagcc acgatagccg cgctgcctcg 5520 tcttgcagtt cattcagggc accggacagg tcggtcttga caaaaagaac cgggcgcccc 5580 tgcgctgaca gccggaacac ggcggcatca gagcagccga ttgtctgttg tgcccagtca 5640 tagccgaata gcctctccac ccaagcggcc ggagaacctg cgtgcaatcc atcttgttca 5700 atcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc 5760 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc 5820 cgaaaagtgc cacctgaacg aagcatctgt gcttcatttt gtagaacaaa aatgcaacgc 5880 gagagcgcta atttttcaaa caaagaatct gagctgcatt tttacagaac agaaatgcaa 5940 cgcgaaagcg ctattttacc aacgaagaat ctgtgcttca tttttgtaaa acaaaaatgc 6000 aacgcgagag cgctaatttt tcaaacaaag aatctgagct gcatttttac agaacagaaa 6060 tgcaacgcga gagcgctatt ttaccaacaa agaatctata cttctttttt gttctacaaa 6120 aatgcatccc gagagcgcta tttttctaac aaagcatctt agattacttt ttttctcctt 6180 tgtgcgctct ataatgcagt ctcttgataa ctttttgcac tgtaggtccg ttaaggttag 6240 aagaaggcta ctttggtgtc tattttctct tccataaaaa aagcctgact ccacttcccg 6300 cgtttactga ttactagcga agctgcgggt gcattttttc aagataaagg catccccgat 6360 tatattctat accgatgtgg attgcgcata ctttgtgaac agaaagtgat agcgttgatg 6420 attcttcatt ggtcagaaaa ttatgaacgg tttcttctat tttgtctcta tatactacgt 6480 ataggaaatg tttacatttt cgtattgttt tcgattcact ctatgaatag ttcttactac 6540 aatttttttg tctaaagagt aatactagag ataaacataa aaaatgtaga ggtcgagttt 6600 agatgcaagt tcaaggagcg aaaggtggat gggtaggtta tatagggata tagcacagag 6660 atatatagca aagagatact tttgagcaat gtttgtggaa gcggtattcg caatatttta 6720 gtagctcgtt acagtccggt gcgtttttgg ttttttgaaa gtgcgtcttc agagcgcttt 6780 tggttttcaa aagcgctctg aagttcctat actttctaga gaataggaac ttcggaatag 6840 gaacttcaaa gcgtttccga aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat 6900 acagctcact gttcacgtcg cacctatatc tgcgtgttgc ctgtatatat atatacatga 6960 gaagaacggc atagtgcgtg tttatgctta aatgcgtact tatatgcgtc tatttatgta 7020 ggatgaaagg tagtctagta cctcctgtga tattatccca ttccatgcgg ggtatcgtat 7080 gcttccttca gcactaccct ttagctgttc tatatgctgc cactcctcaa ttggattagt 7140 ctcatccttc aatgctatca tttcctttga tattggatca tattaagaaa ccattattat 7200 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg 7260 tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta 7320 agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg 7380 gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atagatcaac 7440 gacattacta tatatataat ataggaagca tttaatagaa cagcatcgta atatatgtgt 7500 actttgcagt tatgacgcca gatggcagta gtggaagata ttctttattg aaaaatagct 7560 tgtcacctta cgtacaatct tgatccggag cttttctttt tttgccgatt aagaattaat 7620 tcggtcgaaa aaagaaaagg agagggccaa gagggagggc attggtgact attgagcacg 7680 tgagtatacg tgattaagca cacaaaggca gcttggagta tgtctgttat taatttcaca 7740 ggtagttctg gtccattggt gaaagtttgc ggcttgcaga gcacagaggc cgcagaatgt 7800 gctctagatt ccgatgctga cttgctgggt attatatgtg tgcccaatag aaagagaaca 7860 attgacccgg ttattgcaag gaaaatttca agtcttgtaa aagcatataa aaatagttca 7920 ggcactccga aatacttggt tggcgtgttt cgtaatcaac ctaaggagga tgttttggct 7980 ctggtcaatg attacggcat tgatatcgtc caactgcatg gagatgagtc gtggcaagaa 8040 taccaagagt tcctcggttt gccagttatt aaaagactcg tatttccaaa agactgcaac 8100 atactactca gtgcagcttc acagaaacct cattcgttta ttcccttgtt tgattcagaa 8160 gcaggtggga caggtgaact tttggattgg aactcgattt ctgactgggt tggaaggcaa 8220 gagagccccg aaagcttaca ttttatgtta gctggtggac tgacgccaga aaatgttggt 8280 gatgcgctta gattaaatgg cgttattggt gttgatgtaa gcggaggtgt ggagacaaat 8340 ggtgtaaaag actctaacaa aatagcaaat ttcgtcaaaa atgctaagaa ataggttatt 8400 actgagtagt atttatttaa gtattgtttg tgcacttgcc gatctatgcg gtgtgaaata 8460 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaaacgtt aatattttgt 8520 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg 8580 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt 8640 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct 8700 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt 8760 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa 8820 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc 8880 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc 8940 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gat 8993 //