ID pdECFP standard; circular DNA; ; 6160 BP. DE LMBP 4548. CC Accession number: LMBP 4548 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 2-APR-2009 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 3769..3864 FT /vntifkey="30" FT /label=amp\promoter FT promoter 1480..1532 FT /vntifkey="30" FT /label=lacUV5\promoter FT /note="Escherichia coli lac operon promoter; mutant (lacUV5)" FT rep_origin 4158..4233 FT /vntifkey="33" FT /label=SV40\ori FT /note="bidirectional origin of SV40" FT rep_origin complement(3313..3768) FT /vntifkey="33" FT /label=f1\ori FT /note="Phage f1 origin" FT promoter 3920..4201 FT /vntifkey="29" FT /label=SV40\early\promoter FT /note="Simian virus 40 early promoter (SV40 early)" FT terminator complement(3163..3259) FT /vntifkey="43" FT /label=SV40\polyA\late FT /note="polyA signal of the SV40 late region" FT terminator 3163..3259 FT /vntifkey="43" FT /label=SV40\polyA\early FT /note="polyA signal of the SV40 early region" FT CDS 2582..2887 FT /vntifkey="4" FT /label=ccdB FT /note="B gene of the control of cell death locus of the E. coli F plasmid (ccdB, lethal gene)" FT CDS 1581..2240 FT /vntifkey="4" FT /label=chloramphenicol\resistance FT attenuator 2928..3052 FT /vntifkey="0" FT /label=attR2 FT /note="Bacteriophage lambda attR2 recombination site" FT attenuator complement(1348..1472) FT /vntifkey="0" FT /label=attR1 FT /note="Bacteriophage lambda attR1 recombination site" FT CDS 613..1329 FT /vntifkey="4" FT /label=ECFP FT /note="A. victoria green-fluorescent protein DNA ; enhanced cyan fluorescent variant" FT promoter 1..568 FT /vntifkey="29" FT /label=hCMV-IE\promoter\and\enhancer FT /note="Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer" FT rep_origin 5828..6154 FT /vntifkey="33" FT /label=pMB1\ori FT /note="Escherichia coli plasmid pMB1 origin" FT CDS complement(4266..5126) FT /vntifkey="4" FT /label=ampicillin\resistance FT RBS 3865..3899 FT /vntifkey="32" FT /label=5'\UTR\of\amp,\incl.\RBS SQ Sequence 6160 BP; 1609 A; 1531 C; 1488 G; 1532 t; tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt 120 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600 ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg 660 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc 720 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg 780 ccctggccca ccctcgtgac caccctgacc tggggcgtgc agtgcttcag ccgctacccc 840 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag 900 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag 960 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac 1020 atcctggggc acaagctgga gtacaactac atcagccaca acgtctatat caccgccgac 1080 aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga ggacggcagc 1140 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg 1200 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc 1260 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag 1320 ctgtacaagt ccggactcag atcatcaaca agtttgtaca aaaaagctga acgagaaacg 1380 taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca gactacataa 1440 tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc ccaggcttta 1500 cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg gcgagatttt 1560 caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc gttgatatat 1620 cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa tgtacctata 1680 accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa aataagcaca 1740 agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat ccggaattcc 1800 gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct tgttacaccg 1860 ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac gacgatttcc 1920 ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt 1980 tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg gtgagtttca 2040 ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt ttcaccatgg 2100 gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg 2160 ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag tactgcgatg 2220 agtggcaggg cggggcgtaa agatctggat ccggcttact aaaagccaga taacagtatg 2280 cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat acccgaagta 2340 tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca gcgacagcta 2400 tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac aaccatgcag 2460 aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga agggatggct 2520 gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag ggactggtga 2580 aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt ttgtggatgt 2640 acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg ccagtgcacg 2700 tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg gggatgaaag 2760 ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg gggaagaagt 2820 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg 2880 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga 2940 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat 3000 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gttgatgatc 3060 caccggatct agataactga tcataatcag ccataccaca tttgtagagg ttttacttgc 3120 tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt 3180 tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt 3240 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt 3300 atcttaaggc gtaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt 3360 aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag 3420 aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga 3480 acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg 3540 aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc 3600 ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg 3660 aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc 3720 gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtca ggtggcactt 3780 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt 3840 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagtc 3900 ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg 3960 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg 4020 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc 4080 aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca 4140 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc 4200 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaga 4260 tcgagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 4320 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 4380 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 4440 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 4500 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 4560 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct 4620 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 4680 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 4740 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 4800 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 4860 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa 4920 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 4980 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 5040 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 5100 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 5160 cagggttatt gtcccaccga gaccccattg gggccaatac gcccgcgttt cttccttttc 5220 cccaccccac cccccaagtt cgggtgaagg cccagggctc gcagccaacg tcggggcggc 5280 aggccctgcc atagcctcag gttactcata tatactttag attgatttaa aacttcattt 5340 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta 5400 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg 5460 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc 5520 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag 5580 cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa 5640 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc 5700 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc 5760 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta 5820 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag 5880 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct 5940 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga 6000 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc 6060 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt 6120 atcccctgat tctgtggata accgtattac cgccatgcat 6160 //