ID pEF1-hOMI standard; circular DNA; ; 7483 BP. DE LMBP 4580. CC Accession number: LMBP 4580 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 9-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 508..1651 FT /vntifkey="29" FT /label=hEF1\promoter FT promoter 1668..1684 FT /vntifkey="30" FT /label=T7\promoter FT intron 704..1642 FT /vntifkey="15" FT /label=intron\hEF1\promoter FT CDS 1719..3095 FT /vntifkey="4" FT /label=hOMI FT misc_marker 3114..3155 FT /vntifkey="22" FT /label=V5\epitope FT misc_marker 3165..3182 FT /vntifkey="22" FT /label=His-tag FT terminator 3211..3435 FT /vntifkey="43" FT /label=bovine\growth\hormone\polyA FT rep_origin 3481..3909 FT /vntifkey="33" FT /label=f1\ori FT promoter 3914..4195 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 4152..4227 FT /vntifkey="33" FT /label=SV40\ori FT terminator 5289..5363 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(5289..5363) FT /vntifkey="43" FT /label=SV40\polyA\late FT CDS complement(6609..7469) FT /vntifkey="4" FT /label=ampicillin FT CDS 4319..5113 FT /vntifkey="4" FT /label=neomycin FT /note="Tn5 antibiotic resistance gene; coding sequence" FT rep_origin complement(5677..6068) FT /vntifkey="33" FT /label=pMB1\ori FT promoter complement(5502..5585) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 7483 BP; 1570 A; 1981 C; 2093 G; 1839 t; aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 60 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 120 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 180 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 240 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 300 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 360 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 420 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 480 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 540 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 600 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 660 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 720 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 780 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 840 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 900 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 960 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 1020 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 1080 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 1140 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 1200 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1260 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1320 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1380 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1440 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1500 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1560 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1620 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 1680 tatagggaga cccaagctgg ctaggtaagc ttggtaccat ggctgcgccg agggcggggc 1740 ggggtgcagg ctggagcctt cgggcatggc gggctttggg gggcattcgc tgggggagga 1800 gaccccgttt gacccctgac ctccgggccc tgctgacgtc aggaacttct gacccccggg 1860 cccgagtgac ttatgggacc cccagtctct gggcccggtt gtctgttggg gtcactgaac 1920 cccgagcatg cctgacgtct gggaccccgg gtccccgggc acaactgact gcggtgaccc 1980 cagataccag gacccgggag gcctcagaga actctggaac ccgttcgcgc gcgtggctgg 2040 cggtggcgct gggcgctggg ggggcagtgc tgttgttgtt gtggggcggg ggtcggggtc 2100 ctccggccgt cctcgccgcc gtccctagcc cgccgcccgc ttctccccgg agtcagtaca 2160 acttcatcgc agatgtggtg gagaagacag cacctgccgt ggtctatatc gagatcctgg 2220 accggcaccc tttcttgggc cgcgaggtcc ctatctcgaa cggctcagga ttcgtggtgg 2280 ctgccgatgg gctcattgtc accaacgccc atgtggtggc tgatcggcgc agagtccgtg 2340 tgagactgct aagcggcgac acgtatgagg ccgtggtcac agctgtggat cccgtggcag 2400 acatcgcaac gctgaggatt cagactaagg agcctctccc cacgctgcct ctgggacgct 2460 cagctgatgt ccggcaaggg gagtttgttg ttgccatggg aagtcccttt gcactgcaga 2520 acacgatcac atccggcatt gttagctctg ctcagcgtcc agccagagac ctgggactcc 2580 cccaaaccaa tgtggaatac attcaaactg atgcagctat tgattttgga aactctggag 2640 gtcccctggt taacctggat ggggaggtga ttggagtgaa caccatgaag gtcacagctg 2700 gaatctcctt tgccatccct tctgatcgtc ttcgagagtt tctgcatcgt ggggaaaaga 2760 agaattcctc ctccggaatc agtgggtccc agcggcgcta cattggggtg atgatgctga 2820 ccctgagtcc cagcatcctt gctgaactac agcttcgaga accaagcttt cccgatgttc 2880 agcatggtgt actcatccat aaagtcatcc tgggctcccc tgcacaccgg gctggtctgc 2940 ggcctggtga tgtgattttg gccattgggg agcagatggt acaaaatgct gaagatgttt 3000 atgaagctgt tcgaacccaa tcccagttgg cagtgcagat ccggcgggga cgagaaacac 3060 tgaccttata tgtgacccct gaggtcacag aatgatctag agggcccttc gaaggtaagc 3120 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc 3180 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 3240 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 3300 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 3360 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 3420 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 3480 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 3540 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 3600 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 3660 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 3720 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 3780 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 3840 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 3900 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 3960 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 4020 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 4080 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 4140 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 4200 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 4260 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 4320 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 4380 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 4440 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 4500 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 4560 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 4620 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 4680 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 4740 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 4800 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 4860 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 4920 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 4980 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 5040 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 5100 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 5160 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 5220 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 5280 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 5340 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 5400 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 5460 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 5520 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 5580 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 5640 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 5700 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 5760 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagg 5820 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 5880 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 5940 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 6000 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 6060 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 6120 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 6180 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 6240 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 6300 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 6360 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 6420 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 6480 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 6540 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 6600 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 6660 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 6720 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 6780 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 6840 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 6900 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 6960 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 7020 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 7080 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 7140 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 7200 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 7260 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 7320 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 7380 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 7440 agggcgacac ggaaatgttg aatactcata ctcttccttt ttc 7483 //