LOCUS       pEF1-hOMI-S306A         7483 bp    DNA     circular     11-JAN-2008
DEFINITION  LMBP 4581.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 4581
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 11-JAN-2008
            
COMMENT     VNTNAME|pEF1-hOMI-S306A|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pEF1-hOMI-S306A" 0 7483 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) 
COMMENT        (CStringList "EcoRI" "HindII" "KpnI" "NaeI" "PstI" "SspI" "XbaI" "XmnI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "2mu ori" 
COMMENT             "AGAAAGTATAGGAACTTCAGAGCGCTTTTGAAAACCAAAAGCGCTCTGAAGACGCACTTTCAAAAAACCAAAAACGCACCGGACTGTAACGAGCTACTAAAATATTGCGAATACCGCTTCCACAAACATTGCTCAAAAGTATCTCTTTGCTATATATCTCTGTGCTATATCCCTATATAACCTACCCATCCACCTTTCGCTCCTTGAACTTGCATCTAAACTCGACCTCTACATTTTTTATGTTTATCTCTAGTATTACTCTTTAGACAAAAAAATTGTAGTAAGAACTATTCATAGAGTGAATCGAAAACAATACGAAAATGTAAACATTTCCTATACGTAGTATATAGAGACAAAATAGAAGAAACCGTTCATAATTTTCTGACCAATGAAGAATCATCAACGCTATCACTTTCTGTTCACAAAGTATGCGCAATCCACATCGGTATAGAATATAATCGGGGATGCCTTTATCTTGAAAAAATGCACCCGCAGCTTCGCTAGTAATCAGTAAACGCGGGAAGTGGAGTCAGGCTTTTTTTATGGAAGAGAAAATAGACACCAAAGTAGCCTTCTTCTAACCTTAACGGACCTACAGTGCAAAAAGTTATCAAGAGACTGCATTATAGAGCGCACAAAGGAGAAAAAAAGTAATCTAAGATGCTTTGTTAGAAAAATAGCGCTCTCGGGATGCATTTTTGTAGAACAAAAAAGAAGTATAGATTCTTTGTTGGTAAAATAGCGCTCTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTTTACAAAAATGAAGCACAGATTCTTCGTTGGTAAAATAGCGCTTTCGCGTTGCATTTCTGTTCTGTAAAAATGCAGCTCAGATTCTTTGTTTGAAAAATTAGCGCTCTCGCGTTGCATTTTTGTTCTACAAAATGAAGCACAGATGCTTCGTT" 
COMMENT             "Fragment of pAS3; Length: 981" 0 (CStringList) 0) 
COMMENT         #1=(COligo "5' UTR of AOX1" 
COMMENT             "ACAGCAATATATAAACAGAAGGAAGCTGCCCTGTCTTAAACCTTTTTTTTTATCATCATTATTAGCTTACTTTCATAATTGCGACTGGTTCCAATTGACAAGCTTTTGATTTTAACGACTTTTAACGACAACTTGAGAAGATCAAAAAACAACTAATTATTCGAA" 
COMMENT             "Fragment of pPP1MFmds1; Length: 165" 0 (CStringList) 0) 
COMMENT         #2=(COligo "ADH1 promoter" 
COMMENT             "TGCAACTTCTTTTCTTTTTTTTTCTTTTCTCTCTCCCCCGTTGTTGTCTCACCATATCCGCAATGACAAAAAAAATGATGGAAGACACTAAAGGAAAAAATTAACGACAAAGACAGCACCAACAGATGTCGTTGTTCCAGAGCTGATGAGGGGTATCTCGAAGCACACGAAACTTTTTCCTTCCTTCATTCACGCACACTACTCTCTAATGAGCAACGGTATACGGCCTTCCTTCCAGTTACTTGAATTTGAAATAAAAAAAGTTTGCCGCTTTGCTATCAAGTATAAATAGACCTGCAATTATTAATCTTTTGTTTCCTCGTCATTGTTCTCGTTCCCTTTCTTCCTTGTTTCTTTTTCTGCACAATATTTCAAGCTATACCAAGCATACAATCAACTCCAAG" 
COMMENT             "Fragment of pAS3; Length: 404" 0 (CStringList) 0) 
COMMENT         #3=(COligo "ADH1 terminator" 
COMMENT             "GCGAATTTCTTATGATTTATGATTTTTATTATTAAATAAGTTATAAAAAAAATAAGTGTATACAAATTTTAAAGTGACTCTTAGGTTTTAAAACGAAAATTC" 
COMMENT             "Fragment of pRH007; Length: 102" 0 (CStringList) 0) 
COMMENT         #4=(COligo "AMP promoter onvolledig" 
COMMENT             "CGTCAGGTGGCACTTTTCGGGGAAATGTGCGCGGAACCCCTATTTGTTTATTTTTCTAAATACATTCAAATATGTATCCGCTCATGAGACAATAACCCT" 
COMMENT             "Fragment of pDEST-ECFP; Length: 99 onvolledig" 0 (CStringList) 0) 
COMMENT         #5=(COligo "AMP RBS" "GATAAATGCTTCAATAATATTGAAAAAGGAAGAGT" 
COMMENT             "Fragment of pdECFP; Length: 35" 0 (CStringList) 0) 
COMMENT         #6=(COligo "ampicillin" 
COMMENT             "ATGAGTATTCAACATTTCCGTGTCGCCCTTATTCCCTTTTTTGCGGCATTTTGCCTTCCTGTTTTTGCTCACCCAGAAACGCTGGTGAAAGTAAAAGATGCTGAAGATCAGTTGGGTGCACGAGTGGGTTACATCGAACTGGATCTCAACAGCGGTAAGATCCTTGAGAGTTTTCGCCCCGAAGAACGTTTTCCAATGATGAGCACTTTTAAAGTTCTGCTATGTGGCGCGGTATTATCCCGTATTGACGCCGGGCAAGAGCAACTCGGTCGCCGCATACACTATTCTCAGAATGACTTGGTTGAGTACTCACCAGTCACAGAAAAGCATCTTACGGATGGCATGACAGTAAGAGAATTATGCAGTGCTGCCATAACCATGAGTGATAACACTGCGGCCAACTTACTTCTGACAACGATCGGAGGACCGAAGGAGCTAACCGCTTTTTTGCACAACATGGGGGATCATGTAACTCGCCTTGATCGTTGGGAACCGGAGCTGAATGAAGCCATACCAAACGACGAGCGTGACACCACGATGCCTGTAGCAATGGCAACAACGTTGCGCAAACTATTAACTGGCGAACTACTTACTCTAGCTTCCCGGCAACAATTAATAGACTGGATGGAGGCGGATAAAGTTGCAGGACCACTTCTGCGCTCGGCCCTTCCGGCTGGCTGGTTTATTGCTGATAAATCTGGAGCCGGTGAGCGTGGGTCTCGCGGTATCATTGCAGCACTGGGGCCAGATGGTAAGCCCTCCCGTATCGTAGTTATCTACACGACGGGGAGTCAGGCAACTATGGATGAACGAAATAGACAGATCGCTGAGATAGGTGCCTCACTGATTAAGCATTGGTAA" 
COMMENT             "Fragment of pEF6-E-mTRAF2; Length: 861" 0 (CStringList) 0) 
COMMENT         #7=(COligo "AOX1 promoter" 
COMMENT             "AACATCCAAAGACGAAAGGTTGAATGAAACCTTTTTGCCATCCGACATCCACAGGTCCATTCTCACACATAAGTGCCAAACGCAACAGGAGGGGATACACTAGCAGCAGACCGTTGCAAACGCAGGACCTCCACTCCTCTTCTCCTCAACACCCACTTTTGCCATCGAAAAACCAGCCCAGTTATTGGGCTTGATTGGAGCTCGCTCATTCCAATTCCTTCTATTAGGCTACTAACACCATGACTTTATTAGCCTGTCTATCCTGGCCCCCCTGGCGAGGTTCATGTTTGTTTATTTCCGAATGCAACAAGCTCCGCATTACACCCGAACATCACTCCAGATGAGGGCTTTCTGAGTGTGGGGTCAAATAGTTTCATGTTCCCCAAATGGCCCAAAACTGACAGTTTAAACGCTGTCTTGGAACCTAATATGACAAAAGCGTGATCTCATCCAAGATGAACTAAGTTTGGTTCGTTGAAATGCTAACGGCCAGTTGGTCAAAAAGAAACTTCCAAAAGTCGCCATACCGTTTGTCTTGTTTGGTATTGATTGACGAATGCTCAAAAATAATCTCATTAATGCTTAGCGCAGTCTCTCTATCGCTTCTGAACCCCGGTGCACCTGTGCCGAAACGCAAATGGGGAAACACCCGCTTTTTGGATGATTATGCATTGTCTCCACATTGTATGCTTCCAAGATTCTGGTGGGAATACTGCTGATAGCCTAACGTTCATGATCAAAATTTAACTGTTCTAACCCCTACTTG" 
COMMENT             "Fragment of pPP1MFmds1; Length: 766" 0 (CStringList) 0) 
COMMENT         #8=(COligo "AOX1 terminator" 
COMMENT             "TCAAGAGGATGTCAGAATGCCATTTGCCTGAGAGATGCAGGCTTCATTTTTGATACTTTTTTATTTGTAACCTATATAGTATAGGATTTTTTTTGTCATTTTGTTTCTTCTCGTACGAGCTTGCTCCTGATCAGCCTATCTCGCAGCTGATGAATATCTTGTGGTAGGGGTTTGGGAAAATCATTCGAGTTTGATGTTTTTCTTGGTATTTCCCACTCCTCTTCAGAGTACAGAAGATTAAGTGAGAAGTTCGTTTGTGC" 
COMMENT             "Fragment of pPP1MFmds1; Length: 260" 0 (CStringList) 0) 
COMMENT         #9=(COligo "attR1" 
COMMENT             "CATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTTTTGTACAAACTTGT" 
COMMENT             "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #10=(COligo "attR2" 
COMMENT              "CATAGTGACTGGATATGTTGTGTTTTACAGTATTATGTAGTCTGTTTTTTATGCAAAATCTAATTTAATATATTGATATTTATATCATTTTACGTTTCTCGTTCAGCTTTCTTGTACAAAGTGGT" 
COMMENT              "Fragment of pRH007; Length: 125" 0 (CStringList) 0) 
COMMENT         #11=(COligo "BK ori" "GAGGCCGAGGCCGCCTCTGCCTC" 
COMMENT              "Fragment of pBK-1; Length: 23 " 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #12=(COligo "blasticidin" 
COMMENT              "ATGGCCAAGCCTTTGTCTCAAGAAGAATCCACCCTCATTGAAAGAGCAACGGCTACAATCAACAGCATCCCCATCTCTGAAGACTACAGCGTCGCCAGCGCAGCTCTCTCTAGCGACGGCCGCATCTTCACTGGTGTCAATGTATATCATTTTACTGGGGGACCTTGTGCAGAACTCGTGGTGCTGGGCACTGCTGCTGCTGCGGCAGCTGGCAACCTGACTTGTATCGTCGCGATCGGAAATGAGAACAGGGGCATCTTGAGCCCCTGCGGACGGTGCCGACAGGTGCTTCTCGATCTGCATCCTGGGATCAAAGCCATAGTGAAGGACAGTGATGGACAGCCGACGGCAGTTGGGATTCGTGAATTGCTGCCCTCTGGTTATGTGTGGGAGGGCTAA" 
COMMENT              "Fragment of pEF6-E-mTRAF2; Length: 399" 0 (CStringList) 0) 
COMMENT         #13=(COligo "bovine growth hormone polyA" 
COMMENT              "CTGTGCCTTCTAGTTGCCAGCCATCTGTTGTTTGCCCCTCCCCCGTGCCTTCCTTGACCCTGGAAGGTGCCACTCCCACTGTCCTTTCCTAATAAAATGAGGAAATTGCATCGCATTGTCTGAGTAGGTGTCATTCTATTCTGGGGGGTGGGGTGGGGCAGGACAGCAAGGGGGAGGATTGGGAAGACAATAGCAGGCATGCTGGGGATGCGGTGGGCTCTATGG" 
COMMENT              "Fragment of pEF1hCASP-9S-His; Length: 225" 0 (CStringList) 0) 
COMMENT         #14=(COligo "CaGAL1 promoter (1)" 
COMMENT              "AGATCTGATATTGACGAAGAGGAACCAATGTAACGTTACACTGAAGAAAACACACAATAAACGGGAAGAAACGGTGTAAAAGTGTGAAAATAATTTTTGAATATCATTTCCCTTGGTTTAATTCCAAACGAAACGTGTTTTTTTTAGAGAATGGGAATTCTTATTGGATGTCTAGATTGTTTGTTTACTCCAGACTGTGCACAAAAACGTTTGGATGGATGATCAGAAGATATTTTTAGGCTTAGCTCTAAATATAAGAAATGATGCTTGAAAAACCAGACAGAAATTGAGTTTCAAAAATTGGTAATGTGAGGTATTAGTCAACTAACCAAATAACAATGCAAACCGGTTGATACATTTCATTTTGAAAATAATGAAACTGGAATTGGATGACCAGCACACAAACACATAAAGTAATTATGGGAATTAGAAGCGAACATAGAGGAGTACTTGGCCACGAACAGAATACAAGTGGGAACACTATTTTCTCCATTGTTTTAGTTCTGTTTTTTTGTCAGCCTAGTTTTGTGCTATGTGTAAAAAATATTGCCAAGAAAAAAAGCTTGTTTTGTGGCCAGTGTCCGAAAAAAATTTTGGGGAATCTTCGGATTAATTTATGTTTTCATTCCATCGGGGAAAGTGGGGGGGAAAAAATTTTAAGCAGTTCACAAAACCTTCCAAAAAATATATGGACAAAGATGATTGTATTTTCCCGACACCAAAATCATAATTAATTATGAGAAAGTTAAATGTAACGTTACAATTTATGTTTATTTGAAGGTGAAAAGCGATTTATGATTTTTCCGAAATGAAAATTTTTTTTAGGTTTATTTTTTTTGTCGGGCAAAGAAAAACTGAACAAGGATTATTAAAATTTTTGGTGTTTGTTTGTGTCTGGAGAATTCATTCCTCTCTCATCTTCACACAATGTTTAGACATCTGACACGATTCATGATAGTTCGGTTTCCGGGGTTGGTGTTTAGTTTTCGTTTTTCTTTTT" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 1000" 0 (CStringList) 0) 
COMMENT         #15=(COligo "CaGAL1 promoter (2)" 
COMMENT              "TTTTGGAAAGAATGTTTTAGCTCATTGGTTTTCTTTCTTCATTCAATAGTTTTGAAAGAATTTGCCCACTTGTTATTACAATCATATAAAATTAAACTTTGATATAAAATAGAGTTTGAAAGTTTCCCAGATCCTTTTTGATTTCTTTGTAAATTTTTTTTTCTCCCACATATACACACATACAAACCGATTTTTATAAGAAAGAGTTATACC" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 213" 0 (CStringList) 0) 
COMMENT         #16=(COligo "CaMV promoter" 
COMMENT              "GATCTCCTTTGCCCCGGAGATCACCATGGACGACTTTCTCTATCTCTACGATCTAGGAAGAAAGTTCGACGGAGAAGGTGACGATACCATGTTCACCACCGATAATGAGAAGATTAGCCTCTTCAATTTCAGAAAGAATGCTGACCCACAGATGGTTAGAGAGGCCTACGCGGCAGGTCTCATCAAGACGATCTACCCGAGTAATAATCTCCAGGAGATCAAATACCTTCCCAAGAAGGTTAAAGATGCAGTCAAAAGATTCAGGACTAACTGCATCAAGAACACAGAGAAAGATATATTTCTCAAGATCAGAAGTACTATTCCAGTATGGACGATTCAAGGCTTGCTTCATAAACCAAGGCAAGTAATAGAGATTGGAGTCTCTAAGAAAGTAGTTCCTACTGAATCAAAGGCCATGGAGTCAAAAATTCAGATCGAGGATCTAACAGAACTCGCCGTGAAGACTGGCGAACAGTTCATACAGAGTCTTTTACGACTCAATGACAAGAAGAAAATCTTCGTCAACATGGTGGAGCACGACACTCTCGTCTACTCCAAGAATATCAAAGATACAGTCTCAGAAGACCAAAGGGCTATTGAGACTTTTCAACAAAGGGTAATATCGGGAAACCTCCTCGGATTCCATTGCCCAGCTATCTGTCACTTCATCAAAAGGACAGTAGAAAAGGAAGGTGGCACCTACAAATGCCATCATTGCGATAAAGGAAAGGCTATCGTTCAAGATGCCTCTGCCGACAGTGGTCCCAAAGATGGACCCCCACCCACGAGGAGCATCGTGGAAAAAGAAGACGTTCCAACCACGTCTTCAAAGCAAGTGGATTGATGTGATATCTCCACTGACGTAAGGGATGACGCACAATCCCACTATCCTTCGCAAGACCCTTCCTCTATATAAGGAAGTTCATTTCATTTGGAGAGGACACGCT" 
COMMENT              "Fragment of pEX1SOD; Length: 947" 0 (CStringList) 0) 
COMMENT         #17=(COligo "CaURA3" 
COMMENT              "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT              "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #18=(COligo "ccdB" 
COMMENT              "ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACACGCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGGTGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTGGCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA" 
COMMENT              "Fragment of pRH001; Length: 306" 0 (CStringList) 0) 
COMMENT         #19=(COligo "CEN6/ARSH4 ori" 
COMMENT              "CTTTTCATCACGTGCTATAAAAATAATTATAATTTAAATTTTTTAATATAAATATATAAATTAAAAATAGAAAGTAAAAAAAGAAATTAAAGAAAAAATAGTTTTTGTTTTCCGAAGATGTAAAAGACTCTAGGGGGATCGCCAACAAATACTACCTTTTATCTTGCTCTTCCTGCTCTCAGGTATTAATGCCGAATTGTTTCATCTTGTCTGTGTAGAAGACCACACACGAAAATCCTGTGATTTTACATTTTACTTATCGTTAATCGAATGTATATCTATTTAATCTGCTTTTCTTGTCTAATAAATATATATGTAAAGTACGCTTTTTGTTGAAATTTTTTAAACCTTTGTTTATTTTTTTTTCTTCATTCCGTAACTCTTCTACCTTCTTTATTTACTTTCTAAAATCCAAATACAAAACATAAAAATAAATAAACACAGAGTAAATTCCCAAATTATTCCATCATTAAAAGATACGAGGCGCGTGTAAGTTACAGGCAAGCGATCCGTCCTAAGAAACCA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 525" 0 (CStringList) 0) 
COMMENT         #20=(COligo "chloramphenicol" 
COMMENT              "ATGGAGAAAAAAATCACTGGATATACCACCGTTGATATATCCCAATGGCATCGTAAAGAACATTTTGAGGCATTTCAGTCAGTTGCTCAATGTACCTATAACCAGACCGTTCAGCTGGATATTACGGCCTTTTTAAAGACCGTAAAGAAAAATAAGCACAAGTTTTATCCGGCCTTTATTCACATTCTTGCCCGCCTGATGAATGCTCATCCGGAATTCCGTATGGCAATGAAAGACGGTGAGCTGGTGATATGGGATAGTGTTCACCCTTGTTACACCGTTTTCCATGAGCAAACTGAAACGTTTTCATCGCTCTGGAGTGAATACCACGACGATTTCCGGCAGTTTCTACACATATATTCGCAAGATGTGGCGTGTTACGGTGAAAACCTGGCCTATTTCCCTAAAGGGTTTATTGAGAATATGTTTTTCGTCTCAGCCAATCCCTGGGTGAGTTTCACCAGTTTTGATTTAAACGTGGCCAATATGGACAACTTCTTCGCCCCCGTTTTCACCATGGGCAAATATTATACGCAAGGCGACAAGGTGCTGATGCCGCTGGCGATTCAGGTTCATCATGCCGTCTGTGATGGCTTCCATGTCGGCAGAATGCTTAATGAATTACAACAGTACTGCGATGAGTGGCAGGGCGGGGCGTAA" 
COMMENT              "Fragment of pRH001; Length: 660" 0 (CStringList) 0) 
COMMENT         #21=(COligo "CYC1 terminator" 
COMMENT              "ACAGGCCCCTTTTCCTTTGTCGATATCATGTAATTAGTTATGTCACGCTTACATTCACGCCCTCCCCCCACATCCGCTCTAACCGAAAAGGAAGGAGTTAGACAACCTGAAGTCTAGGTCCCTATTTATTTTTTTATAGTTATGTTAGTATTAAGAACGTTATTTATATTTCAAATTTTTCTTTTTTTTCTGTACAGACGCGTGTACGCATGTAACATTATACTGAAAACCTTGCTTGAGAAGGTTTTGGGACGCTCGAAGGCTTTAATTTGCAAG" 
COMMENT              "Fragment of pAS2; Length: 276  (laatste N: G (276) ipv A (275) -> midden HindIII)" 
COMMENT              0 (CStringList) 0) 
COMMENT         #22=(COligo "CYH2" 
COMMENT              "ATGCCTTCCAGATTCACTAAGACTAGAAAGCACAGAGGTCACGTCTCAGCCGGTAAAGGTCGTATCGGTAAGCACAGAAAGCACCCCGGTGGTAGAGGTATGGCCGGTGGTCAACATCACCACAGAATTAACATGGATAAATACCATCCAGGTTATTTCGGTAAGGTTGGTATGAGATACTTCCACAAGCAACAAGCTCATTTCTGGAAGCCAGTCTTGAACTTGGACAAATTGTGGACATTGATCCCAGAAGACAAGAGAGACCAATACTTGAAATCTGCTTCTAAGGAAACTGCTCCAGTTATTGACACTTTGGCAGCCGGTTACGGTAAGATCTTGGGTAAGGGTAGAATTCCAAATGTTCCAGTTATCGTCAAAGCTAGATTCGTCTCCAAGTTGGCTGAAGAAAAAATCAGAGCTGCTGGTGGTGTTGTTGAATTGATCGCTTAA" 
COMMENT              "Fragment of pAS2; Length: 450" 0 (CStringList) 0) 
COMMENT         #23=(COligo "E-tag" "GGNGCNCCNGTNCCNTAYCCNGAYCCNYTNGARCCNCGN" 
COMMENT              " Fragment of pYD1TSE ; Length: 39" 0 (CStringList) 0) 
COMMENT         #24=(COligo "ECFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTGGGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACATCAGCCACAACGTCTATATCACCGCCGACAAGCAGAAGAACGGCATCAAGGCCAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pECFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #25=(COligo "EGFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCCTGACCTACGGCGTGCAGTGCTTCAGCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCACCCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEGFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #26=(COligo "EK recognition site" "GAYGAYGAYGAYAAR" "Length: 15 bp" 0 
COMMENT              (CStringList) 0) 
COMMENT         #27=(COligo "EM7 promoter" 
COMMENT              "GTTGACAATTAATCATCGGCATAGTATATCGGCATAGTATAATACGACAAGGTGAGGAACTAAACC" 
COMMENT              "Fragment of pPICZTS; Length: 66, as in the pEF6 plasmids" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #28=(COligo "EYFP" 
COMMENT              "ATGGTGAGCAAGGGCGAGGAGCTGTTCACCGGGGTGGTGCCCATCCTGGTCGAGCTGGACGGCGACGTAAACGGCCACAAGTTCAGCGTGTCCGGCGAGGGCGAGGGCGATGCCACCTACGGCAAGCTGACCCTGAAGTTCATCTGCACCACCGGCAAGCTGCCCGTGCCCTGGCCCACCCTCGTGACCACCTTCGGCTACGGCCTGCAGTGCTTCGCCCGCTACCCCGACCACATGAAGCAGCACGACTTCTTCAAGTCCGCCATGCCCGAAGGCTACGTCCAGGAGCGCACCATCTTCTTCAAGGACGACGGCAACTACAAGACCCGCGCCGAGGTGAAGTTCGAGGGCGACACCCTGGTGAACCGCATCGAGCTGAAGGGCATCGACTTCAAGGAGGACGGCAACATCCTGGGGCACAAGCTGGAGTACAACTACAACAGCCACAACGTCTATATCATGGCCGACAAGCAGAAGAACGGCATCAAGGTGAACTTCAAGATCCGCCACAACATCGAGGACGGCAGCGTGCAGCTCGCCGACCACTACCAGCAGAACACCCCCATCGGCGACGGCCCCGTGCTGCTGCCCGACAACCACTACCTGAGCTACCAGTCCGCCCTGAGCAAAGACCCCAACGAGAAGCGCGATCACATGGTCCTGCTGGAGTTCGTGACCGCCGCCGGGATCACTCTCGGCATGGACGAGCTGTACAAGTAA" 
COMMENT              "Fragment of pEYFP-1; Length: 720" 0 (CStringList) 0) 
COMMENT         #29=(COligo "f1 ori" 
COMMENT              "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTAGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGTCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT              "Fragment of pAS2; Length: 456" 0 (CStringList) 0) 
COMMENT         #30=(COligo "fd terminator" 
COMMENT              "TAAACCGATACAATTAAAGGCTCCTTTTGGAGCCTTTTTTTTTGGAGATTTTCAACGTGAAAAAATTATTATTCGCAATTCC" 
COMMENT              "Fragment of bacteriophage_fd; Length: 82, zoals in pMa58" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #31=(COligo "FLAG-tag" "GAYTAYAARGAYGAYGAYGAYAAR" 
COMMENT              " Fragment of pCAGGS-FLAGmA20; Length: 24" 0 (CStringList) 0) 
COMMENT         #32=(COligo "FLD1 promoter start" 
COMMENT              "GCATGCAGGAATCTCTGGCACGGTGCTAATGGTAGTTATCCAACGGAGCTGAGGTAGTCGATATATCTGGATATGCCGCCTATAGGATAAAAACAGGAGA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #33=(COligo "FLD1 promoter stop" 
COMMENT              "TTTAGAGCCTTACCTGATCGCAATCAGGATTTCACTACTCATATAAATACATCGCTCAAAGCTCCAACTTTGCTTGTTCATACAATTCTTGATATTCACA" 
COMMENT              "Fragment of pFLDalpha; Length: 100" 0 (CStringList) 0) 
COMMENT         #34=(COligo "FLP terminator" 
COMMENT              "GTACGCATTTAAGCATAAACACGCACTATGCCGTTCTTCTCATGTATATATATATACAGGCAACACGCAGATATAGGTGCGACGTGAACAGTGAGCTGTATGTGC" 
COMMENT              "Fragment of pSCGALyEGFP1 compl; Length: 105" 0 (CStringList) 0) 
COMMENT         #35=(COligo "GAL1 promoter" 
COMMENT              "AAGCCGCCGAGCGGGTGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 384" 0 (CStringList) 0) 
COMMENT         #36=(COligo "GAL10/CYC1 promoter" 
COMMENT              "ATAACCATAAAAGCTAGTATTGTAGAATCTTTATTGTTCGGAGCAGTGCGGCGCGAGGCACATCTGCGTTTCAGGAACGCGACCGGTGAAGACGAGGACGCACGGAGGAGAGTCTTCCTTCGGAGGGCTGTCACCCGCTCGGCGGCTTCTAATCCGTACTTCAATATAGCAATGAGCAGTTAAGCGTATTACTGAAAGTTCCAAAGAGAAGGTTTTTTTAGGCTAATCGACCTCGAGCAGATCCGCCAGGCGTGTATATAGCGTGGATGGCCAGGCAACTTTAGTGCTGACACATACAGGCATATATATATGTGTGCGACGACACATGATCATATGGCATGCATGTGCTCTGTATGTATATAAAACTCTTGTTTTCTTCTTTTCTCTAAATATTCTTTCCTTATACAT" 
COMMENT              "Fragment of pEMBLyex4; Length: 408" 0 (CStringList) 0) 
COMMENT         #37=(COligo "GALad" 
COMMENT              "ATGGATAAAGCGGAATTAATTCCCGAGCCTCCAAAAAAGAAGAGAAAGGTCGAATTGGGTACCGCCGCCAATTTTAATCAAAGTGGGAATATTGCTGATAGCTCATTGTCCTTCACTTTCACTAACAGTAGCAACGGTCCGAACCTCATAACAACTCAAACAAATTCTCAAGCGCTTTCACAACCAATTGCCTCCTCTAACGTTCATGATAACTTCATGAATAATGAAATCACGGCTAGTAAAATTGATGATGGTAATAATTCAAAACCACTGTCACCTGGTTGGACGGACCAAACTGCGTATAACGCGTTTGGAATCACTACAGGGATGTTTAATACCACTACAATGGATGATGTATATAACTATCTATTCGATGATGAAGATACCCCACCAAACCCAAAAAAAGAG" 
COMMENT              "Fragment of pGAD-mCASP-11; Length: 408" 0 (CStringList) 0) 
COMMENT         #38=(COligo "GALbd" 
COMMENT              "ATGAAGCTACTGTCTTCTATCGAACAAGCATGCGATATTTGCCGACTTAAAAAGCTCAAGTGCTCCAAAGAAAAACCGAAGTGCGCCAAGTGTCTGAAGAACAACTGGGAGTGTCGCTACTCTCCCAAAACCAAAAGGTCTCCGCTGACTAGGGCACATCTGACAGAAGTGGAATCAAGGCTAGAAAGACTGGAACAGCTATTTCTACTGATTTTTCCTCGAGAAGACCTTGACATGATTTTGAAAATGGATTCTTTACAGGATATAAAAGCATTGTTAACAGGATTATTTGTACAAGATAATGTGAATAAAGATGCCGTCACAGATAGATTGGCTTCAGTGGAGACTGATATGCCTCTAACATTGAGACAGCATAGAATAAGTGCGACATCATCATCGGAAGAGAGTAGTAACAAAGGTCAAAGACAGTTGACTGTATCG" 
COMMENT              "Fragment of pAS3; Length: 441" 0 (CStringList) 0) 
COMMENT         #39=(COligo "GALL promoter" 
COMMENT              "TGACAGCCCTCCGAAGGAAGACTCTCCTCCGTGCGTCCTCGTCTTCACCGGTCGCGTTCCTGAAACGCAGATGTGCCTCGCGCCGCACTGCTCCGAACAATAAAGATTCTACAATACTAGCTTTTATGGTTATGAAGAGGAAAAATTGGCAGTAACCTGGCCCCACAAACCTTCAAATGAACGAATCAAATTAACAACCATAGGATGATAATGCGATTAGTTTTTTAGCCTTATTTCTGGGGTAATTAATCAGCGAAGCGATGATTTTTGATCTATTAACAGATATATAAATGCAAAAACTGCATAACCACTTTAACTAATACTTTCAACATTTTCGGTTTGTATTACTTCTTATTCAAATGTAATAAA" 
COMMENT              "Fragment of p415GALLmBAX; Length: 369" 0 (CStringList) 0) 
COMMENT         #40=(COligo "GAP promoter" 
COMMENT              "AGATCTTTTTTGTAGAAATGTCTTGGTGTCCTCGTCCAATCAGGTAGCCATCTCTGAAATATCTGGCTCCGTTGCAACTCCGAACGACCTGCTGGCAACGTAAAATTCTCCGGGGTAAAACTTAAATGTGGAGTAATGGAACCAGAAACGTCTCTTCCCTTCTCTCTCCTTCCACCGCCCGTTACCGTCCCTAGGAAATTTTACTCTGCTGGAGAGCTTCTTCTACGGCCCCCTTGCAGCAATGCTCTTCCCAGCATTACGTTGCGGGTAAAACGGAGGTCGTGTACCCGACCTAGCAGCCCAGGGATGGAAAAGTCCCGGCCGTCGCTGGCAATAATAGCGGGCGGACGCATGTCATGAGATTATTGGAAACCACCAGAATCGAATATAAAAGGCGAACACCTTTCCCAATTTTGGTTTCTCCTGACCCAAAGACTTTAAATTTAATTTATTTGTCCCTATTTCAATCAATTGAACAACTAT" 
COMMENT              "Fragment of pGAPZMFManHDEL; Length: 483" 0 (CStringList) 0) 
COMMENT         #41=(COligo "GST" 
COMMENT              "ATGTCCCCTATACTAGGTTATTGGAAAATTAAGGGCCTTGTGCAACCCACTCGACTTCTTTTGGAATATCTTGAAGAAAAATATGAAGAGCATTTGTATGAGCGCGATGAAGGTGATAAATGGCGAAACAAAAAGTTTGAATTGGGTTTGGAGTTTCCCAATCTTCCTTATTATATTGATGGTGATGTTAAATTAACACAGTCTATGGCCATCATACGTTATATAGCTGACAAGCACAACATGTTGGGTGGTTGTCCAAAAGAGCGTGCAGAGATTTCAATGCTTGAAGGAGCGGTTTTGGATATTAGATACGGTGTTTCGAGAATTGCATATAGTAAAGACTTTGAAACTCTCAAAGTTGATTTTCTTAGCAAGCTACCTGAAATGCTGAAAATGTTCGAAGATCGTTTATGTCATAAAACATATTTAAATGGTGATCATGTAACCCATCCTGACTTCATGTTGTATGACGCTCTTGATGTTGTTTTATACATGGACCCAATGTGCCTGGATGCGTTCCCAAAATTAGTTTGTTTTAAAAAACGTATTGAAGCTATCCCACAAATTGATAAGTACTTGAAATCCAGCAAGTATATAGCATGGCCTTTGCAGGGCTGGCAAGCCACGTTTGGTGGTGGCGACCATCCTCCAAAA" 
COMMENT              "Fragment of pGEXGSTp65(12-317); Length: 654" 0 (CStringList) 0) 
COMMENT         #42=(COligo "GUS start" 
COMMENT              "ATGGTCCGTCCTGTAGAAACCCCAACCCGTGAAATCAAAAAACTCGACGGCCTGTGGGCATTCAGTCTGGATCGCGAAAACTGTGGAATTGATCAGCGTT" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #43=(COligo "GUS stop" 
COMMENT              "CACTCGCGACCGCAAACCGAAGTCGGCGGCTTTTCTGCTGCAAAAACGCTGGACTGGCATGAACTTCGGTGAAAAACCGCAGCAGGGAGGCAAACAATAA" 
COMMENT              "Fragment of pENTR-gus; Length: 100" 0 (CStringList) 0) 
COMMENT         #44=(COligo "HA epitope" "TAYCCNTAYGAYGTNCCNGAYTAYGCN" 
COMMENT              "Fragment of pAS2; Length: 27" 0 (CStringList) 0) 
COMMENT         #45=(COligo "hCMV-IE promoter and enhancer" 
COMMENT              "GACATTGATTATTGACTAGTTATTAATAGTAATCAATTACGGGGTCATTAGTTCATAGCCCATATATGGAGTTCCGCGTTACATAACTTACGGTAAATGGCCCGCCTGGCTGACCGCCCAACGACCCCCGCCCATTGACGTCAATAATGACGTATGTTCCCATAGTAACGCCAATAGGGACTTTCCATTGACGTCAATGGGTGGACTATTTACGGTAAACTGCCCACTTGGCAGTACATCAAGTGTATCATATGCCAAGTACGCCCCCTATTGACGTCAATGACGGTAAATGGCCCGCCTGGCATTATGCCCAGTACATGACCTTATGGGACTTTCCTACTTGGCAGTACATCTACGTATTAGTCATCGCTATTACCATGGTGATGCGGTTTTGGCAGTACATCAATGGGCGTGGATAGCGGTTTGACTCACGGGGATTTCCAAGTCTCCACCCCATTGACGTCAATGGGAGTTTGTTTTGGCACCAAAATCAACGGGACTTTCCAAAATGTCGTAACAACTCCGCCCCATTGACGCAAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCT" 
COMMENT              "Fragment of pCDM8; Length: 584" 0 (CStringList) 0) 
COMMENT         #46=(COligo "hEF1 promoter (1)" 
COMMENT              "GCCCACAGTCCCCGAGAAGTTGGGGGGAGGGGTCGGCAATTGAACCGGTGCCTAGAGAAGGTGGCGCGGGGTAAACTGGGAAAGTGATGTCGTGTACTGGCTCCGCCTTTTTCCCGAGGGTGGGGGAGAACCGTATATAAGTGCAGTAGTCGCCGTGAACGTTCTTTTTCGCAACGGGTTTGCCGCCAGAACACAGGTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACT" 
COMMENT              "Fragment of pEF1-hOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #47=(COligo "hEF1 promoter (2)" 
COMMENT              "GAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAGGTGTCGTGA" 
COMMENT              "Fragment of pEF1-hOMI; Length: 144" 0 (CStringList) 0) 
COMMENT         #48=(COligo "His-tag" "CAYCAYCAYCAYCAYCAY" "" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #49=(COligo "HIS3 start" 
COMMENT              "ATGACAGAGCAGAAAGCCCTAGTAAAGCGTATTACAAATGAAACCAAGATTCAGATTGCGATCTCTTTAAAGGGTGGTCCCCTAGCGATAGAGCACTCGA" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #50=(COligo "HIS3 stop" 
COMMENT              "TAGTGAGAGTGCGTTCAAGGCTCTTGCGGTTGCCATAAGAGAAGCCACCTCGCCCAATGGTACCAACGATGTTCCCTCCACCAAAGGTGTTCTTATGTAG" 
COMMENT              "Fragment of p413 GAL1; Length: 100" 0 (CStringList) 0) 
COMMENT         #51=(COligo "hOMI (1)" 
COMMENT              "ATGGCTGCGCCGAGGGCGGGGCGGGGTGCAGGCTGGAGCCTTCGGGCATGGCGGGCTTTGGGGGGCATTCGCTGGGGGAGGAGACCCCGTTTGACCCCTGACCTCCGGGCCCTGCTGACGTCAGGAACTTCTGACCCCCGGGCCCGAGTGACTTATGGGACCCCCAGTCTCTGGGCCCGGTTGTCTGTTGGGGTCACTGAACCCCGAGCATGCCTGACGTCTGGGACCCCGGGTCCCCGGGCACAACTGACTGCGGTGACCCCAGATACCAGGACCCGGGAGGCCTCAGAGAACTCTGGAACCCGTTCGCGCGCGTGGCTGGCGGTGGCGCTGGGCGCTGGGGGGGCAGTGCTGTTGTTGTTGTGGGGCGGGGGTCGGGGTCCTCCGGCCGTCCTCGCCGCCGTCCCTAGCCCGCCGCCCGCTTCTCCCCGGAGTCAGTACAACTTCATCGCAGATGTGGTGGAGAAGACAGCACCTGCCGTGGTCTATATCGAGATCCTGGACCGGCACCCTTTCTTGGGCCGCGAGGTCCCTATCTCGAACGGCTCAGGATTCGTGGTGGCTGCCGATGGGCTCATTGTCACCAACGCCCATGTGGTGGCTGATCGGCGCAGAGTCCGTGTGAGACTGCTAAGCGGCGACACGTATGAGGCCGTGGTCACAGCTGTGGATCCCGTGGCAGACATCGCAACGCTGAGGATTCAGACTAAGGAGCCTCTCCCCACGCTGCCTCTGGGACGCTCAGCTGATGTCCGGCAAGGGGAGTTTGTTGTTGCCATGGGAAGTCCCTTTGCACTGCAGAACACGATCACATCCGGCATTGTTAGCTCTGCTCAGCGTCCAGCCAGAGACCTGGGACTCCCCCAAACCAATGTGGAATACATTCAAACTGATGCAGCTATTGATTTTGGAAACTCTGGAGGTCCCCTGGTTAACCTGGATGGGGAGGTGATTGGAGTGAACACCATGAAGGTCACAGCTGGAATCTCCTTTGCCATCC" 
COMMENT              "Fragment of pEF1-hOMI; Length: 1000" 0 (CStringList) 0) 
COMMENT         #52=(COligo "hOMI (2)" 
COMMENT              "CTTCTGATCGTCTTCGAGAGTTTCTGCATCGTGGGGAAAAGAAGAATTCCTCCTCCGGAATCAGTGGGTCCCAGCGGCGCTACATTGGGGTGATGATGCTGACCCTGAGTCCCAGCATCCTTGCTGAACTACAGCTTCGAGAACCAAGCTTTCCCGATGTTCAGCATGGTGTACTCATCCATAAAGTCATCCTGGGCTCCCCTGCACACCGGGCTGGTCTGCGGCCTGGTGATGTGATTTTGGCCATTGGGGAGCAGATGGTACAAAATGCTGAAGATGTTTATGAAGCTGTTCGAACCCAATCCCAGTTGGCAGTGCAGATCCGGCGGGGACGAGAAACACTGACCTTATATGTGACCCCTGAGGTCACAGAATGA" 
COMMENT              "Fragment of pEF1-hOMI; Length: 377" 0 (CStringList) 0) 
COMMENT         #53=(COligo "HSV-TK polyA" 
COMMENT              "AACACGGAAGGAGACAATACCGGAAGGAACCCGCGCTATGACGGCAATAAAAAGACAGAATAAAACGCAC" 
COMMENT              "Fragment of pEYFP-N1; Length: 70" 0 (CStringList) 0) 
COMMENT         #54=(COligo "HSV-TK promoter" 
COMMENT              "CCCCGCCCAGCGTCTTGTCATTGGCGAATTCGAACACGCAGATGCAGTCGGGGCGGCGCGGTCCCAGGTCCACTTCGCATATTAAGGTGACGCGTGTGGCCTCGA" 
COMMENT              "Fragment of p220.2; Length: 105" 0 (CStringList) 0) 
COMMENT         #55=(COligo "intron AG promoter start" 
COMMENT              "GTGAGCGGGCGGGACGGCCCTTCTCCTCCGGGCTGTAATTAGCGCTTGGTTTAATGACGGCTTGTTTCTTTTCTGTGGCTGCGTGAAAGCCTTGAGGGGC" 
COMMENT              "Fragment of pCAGGS; Length: 100" 0 (CStringList) 0) 
COMMENT         #56=(COligo "intron AG promoter stop" 
COMMENT              "TCGGGGGGGACGGGGCAGGGCGGGGTTCGGCTTCTGGCGTGTGACCGGCGGCTCTAGAGCCTCTGCTAACCATGTTCATGCCTTCTTCTTTTTCCTACAG" 
COMMENT              "Fragment of pCAGGS; Length: 100" 0 (CStringList) 0) 
COMMENT         #57=(COligo "intron hEF1 promoter" 
COMMENT              "GTAAGTGCCGTGTGTGGTTCCCGCGGGCCTGGCCTCTTTACGGGTTATGGCCCTTGCGTGCCTTGAATTACTTCCACCTGGCTGCAGTACGTGATTCTTGATCCCGAGCTTCGGGTTGGAAGTGGGTGGGAGAGTTCGAGGCCTTGCGCTTAAGGAGCCCCTTCGCCTCGTGCTTGAGTTGAGGCCTGGCCTGGGCGCTGGGGCCGCCGCGTGCGAATCTGGTGGCACCTTCGCGCCTGTCTCGCTGCTTTCGATAAGTCTCTAGCCATTTAAAATTTTTGATGACCTGCTGCGACGCTTTTTTTCTGGCAAGATAGTCTTGTAAATGCGGGCCAAGATCTGCACACTGGTATTTCGGTTTTTGGGGCCGCGGGCGGCGACGGGGCCCGTGCGTCCCAGCGCACATGTTCGGCGAGGCGGGGCCTGCGAGCGCGGCCACCGAGAATCGGACGGGGGTAGTCTCAAGCTGGCCGGCCTGCTCTGGTGCCTGGCCTCGCGCCGCCGTGTATCGCCCCGCCCTGGGCGGCAAGGCTGGCCCGGTCGGCACCAGTTGCGTGAGCGGAAAGATGGCCGCTTCCCGGCCCTGCTGCAGGGAGCTCAAAATGGAGGACGCGGCGCTCGGGAGAGCGGGCGGGTGAGTCACCCACACAAAGGAAAAGGGCCTTTCCGTCCTCAGCCGTCGCTTCATGTGACTCCACGGAGTACCGGGCGCCGTCCAGGCACCTCGATTAGTTCTCGAGCTTTTGGAGTACGTCGTCTTTAGGTTGGGGGGAGGGGTTTTATGCGATGGAGTTTCCCCACACTGAGTGGGTGGAGACTGAAGTTAGGCCAGCTTGGCACTTGATGTAATTCTCCTTGGAATTTGCCCTTTTTGAGTTTGGATCTTGGTTCATTCTCAAGCCTCAGACAGTGGTTCAAAGTTTTTTTCTTCCATTTCAG" 
COMMENT              "Fragment of pEF1-hOMI; Length: 939" 0 (CStringList) 0) 
COMMENT         #58=(COligo "IRES start" 
COMMENT              "CCCCCCCCCCCCCCCCCCCTCTCCCTCCCCCCCCCCTAACGTTACTGGCCGAAGCCGCTTGGAATAAGGCCGGTGTGCGTTTGTCTATATGTTATTTTCC" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #59=(COligo "IRES stop" 
COMMENT              "CTCGGTGCACATGCTTTACATGTGTTTAGTCGAGGTTAAAAAACGTCTAGGCCCCCCGAACCACGGGGACGTGGTTTTCCTTTGAAAAACACGATGATAA" 
COMMENT              "Fragment of aaapUHGIRES-lacZ; Length: 100" 0 (CStringList) 0) 
COMMENT         #60=(COligo "kanamycin" 
COMMENT              "ATGAGCCATATTCAACGGGAAACGTCTTGCTCAAGGCCGCGATTAAATTCCAACATGGATGCTGATTTATATGGGTATAAATGGGCTCGCGATAATGTCGGGCAATCAGGTGCGACAATCTATCGATTGTATGGGAAGCCCGATGCGCCAGAGTTGTTTCTGAAACATGGCAAAGGTAGCGTTGCCAATGATGTTACAGATGAGATGGTCAGACTAAACTGGCTGACGGAATTTATGCCTCTTCCGACCATCAAGCATTTTATCCGTACTCCTGATGATGCATGGTTACTCACCACTGCGATCCCCGGAAAAACAGCATTCCAGGTATTAGAAGAATATCCTGATTCAGGTGAAAATATTGTTGATGCGCTGGCAGTGTTCCTGCGCCGGTTGCATTCGATTCCTGTTTGTAATTGTCCTTTTAACAGCGATCGCGTATTTCGTCTCGCTCAGGCGCAATCACGAATGAATAACGGTTTGGTTGATGCGAGTGATTTTGATGACGAGCGTAATGGCTGGCCTGTTGAACAAGTCTGGAAAGAAATGCATAAGTTTTTGCCATTCTCACCGGATTCAGTCGTCACTCATGGTGATTTCTCACTTGATAACCTTATTTTTGACGAGGGGAAATTAATAGGTTGTATTGATGTTGGACGAGTCGGAATCGCAGACCGATACCAGGATCTTGCCATCCTATGGAACTGCCTCGGTGAGTTTTCTCCTTCATTACAGAAACGGCTTTTTCAAAAATATGGTATTGATAATCCTGATATGAATAAATTGCAGTTTCATTTGATGCTCGATGAGTTTTTCTAA" 
COMMENT              "Fragment of pRH001; Length: 816" 0 (CStringList) 0) 
COMMENT         #61=(COligo "lac promoter" 
COMMENT              "GCGCAACGCAATTAATGTGAGTTAGCTCACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATGTTGTGTGG" 
COMMENT              "from pUC18; 84 bp." 0 (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #62=(COligo "lacA" 
COMMENT              "TTGAACATGCCAATGACCGAAAGAATAAGAGCAGGCAAGCTATTTACCGATATGTGCGAAGGCTTACCGGAAAAAAGACTTCGTGGGAAAACGTTAATGTATGAGTTTAATCACTCGCATCCATCAGAAGTTGAAAAAAGAGAAAGCCTGATTAAAGAAATGTTTGCCACGGTAGGGGAAAACGCCTGGGTAGAACCGCCTGTCTATTTCTCTTACGGTTCCAACATCCATATAGGCCGCAATTTTTATGCAAATTTCAATTTAACCATTGTCGATGACTACACGGTAACAATCGGTGATAACGTACTGATTGCACCCAACGTTACTCTTTCCGTTACGGGACACCCTGTACACCATGAATTGAGAAAAAACGGCGAGATGTACTCTTTTCCGATAACGATTGGCAATAACGTCTGGATCGGAAGTCATGTGGTTATTAATCCAGGCGTCACCATCGGGGATAATTCTGTTATTGGCGCGGGTAGTATCGTCACAAAAGACATTCCACCAAACGTCGTGGCGGCTGGCGTTCCTTGTCGGGTTATTCGCGAAATAAACGACCGGGATAAGCACTATTATTTCAAAGATTATAAAGTTGAATCGTCAGTTTAA" 
COMMENT              "Fragment of pLacDN10HIFNG; Length: 612" 0 (CStringList) 0) 
COMMENT         #63=(COligo "lacI promoter" 
COMMENT              "GACACCATCGAATGGCGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of pREP4; Length: 78" 0 (CStringList) 0) 
COMMENT         #64=(COligo "lacI start" 
COMMENT              "GTGAAACCAGTAACGTTATACGATGTCGCAGAGTATGCCGGTGTCTCTTATCAGACCGTTTCCCGCGTGGTGAACCAGGCCAGCCACGTTTCTGCGAAAA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #65=(COligo "lacI stop" 
COMMENT              "CACCCTGGCGCCCAATACGCAAACCGCCTCTCCCCGCGCGTTGGCCGATTCATTAATGCAGCTGGCACGACAGGTTTCCCGACTGGAAAGCGGGCAGTGA" 
COMMENT              "Fragment of p714; Length: 100" 0 (CStringList) 0) 
COMMENT         #66=(COligo "lacIq promoter" 
COMMENT              "GACACCATCGAATGGTGCAAAACCTTTCGCGGTATGGCATGATAGCGCCCGGAAGAGAGTCAATTCAGGGTGGTGAAT" 
COMMENT              "Fragment of p714; Length: 78" 0 (CStringList) 0) 
COMMENT         #67=(COligo "lacUV5 promoter" 
COMMENT              "CACTCATTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAATGTGTGG" 
COMMENT              "Fragment of p714; Length: 57" 0 (CStringList) 0) 
COMMENT         #68=(COligo "lacZ RBS" "AATTGTGAGCGGATAACAATTTCACACAGGAAACAGCT" 
COMMENT              "Fragment of pGBF-NLScre; Length: 38" 0 (CStringList) 0) 
COMMENT         #69=(COligo "lacZ start" 
COMMENT              "ATGGGGCCCAAGAAGAAACGCAAAGTGGGGAGCATGGGGGATCCCGTCGTTTTACAACGTCGTGACTGGGAAAACCCTGGCGTTACCCAACTTAATCGCC" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #70=(COligo "lacZ stop" 
COMMENT              "GGGGATTGGTGGCGACGACTCCTGGAGCCCGTCAGTATCGGCGGAATTACAGCTGAGCGCCGGTCGCTACCATTACCAGTTGGTCTGGTGTCAAAAATAA" 
COMMENT              "Fragment of pBI-G; Length: 100" 0 (CStringList) 0) 
COMMENT         #71=(COligo "LEU2 start" 
COMMENT              "ATGTTTGTTCCTAAGAAGATCGTCGTTTTGCCAGGTGACCACGTTGGTCAAGAAATCACAGCCGAAGCCATTAAGGTTCTTAAAGCTATTTCTGATGTTC" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #72=(COligo "LEU2 stop" 
COMMENT              "TTTGGATGCAGGTATCAGAACTGGTGATTTAGGTGGTTCCAACAGTACCACCGAAGTCGGTGATGCTGTCGCCGAAGAAGTTAAGAAAATCCTTGCTTAA" 
COMMENT              "Fragment of pSCGALyEGFP1; Length: 100" 0 (CStringList) 0) 
COMMENT         #73=(COligo "loxP" "ATAACTTCGTATAGCATACATTATACGAAGTT" 
COMMENT              "Fragment of pCALNL5; Length: 32" 0 (CStringList) 0) 
COMMENT         #74=(COligo "LUC start" 
COMMENT              "ATGGAAGACGCCAAAAACATAAAGAAAGGCCCGGCGCCATTCTATCCGCTAGAGGATGGAACCGCTGGAGAGCAACTGCATAAGGCTATGAAGAGATACG" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #75=(COligo "LUC stop" 
COMMENT              "TGTGGACGAAGTACCGAAAGGTCTTACCGGAAAACTCGACGCAAGAAAAATCAGAGAGATCCTCATAAAGGCCAAGAAGGGCGGAAAGTCCAAATTGTAA" 
COMMENT              "Fragment of pBI-GL; Length: 100" 0 (CStringList) 0) 
COMMENT         #76=(COligo "M13 ori" 
COMMENT              "ACGCGCCCTGTAGCGGCGCATTAAGCGCGGCGGGTGTGGTGGTTACGCGCAGCGTGACCGCTACACTTGCCAGCGCCCTAGCGCCCGCTCCTTTCGCTTTCTTCCCTTCCTTTCTCGCCACGTTCGCCGGCTTTCCCCGTCAAGCTCTAAATCGGGGGCTCCCTTTAGGGTTCCGATTTAGTGCTTTACGGCACCTCGACCCCAAAAAACTTGATTTGGGTGATGGTTCACGTAGTGGGCCATCGCCCTGATAGACGGTTTTTCGCCCTTTGACGTTGGAGTCCACGTTCTTTAATAGTGGACTCTTGTTCCAAACTGGAACAACACTCAACCCTATCTCGGGCTATTCTTTTGATTTATAAGGGATTTTGCCGATTTCGGCCTATTGGTTAAAAAATGAGCTGATTTAACAAAAATTTAACGCGAATTTTAACAAAATATTAACGTTTACAATTT" 
COMMENT              "Fragment of pCS4; Length: 456" 0 (CStringList) 0) 
COMMENT         #77=(COligo "mBAX" 
COMMENT              "ATGGACGGGTCCGGGGAGCAGCTTGGGAGCGGCGGGCCCACCAGCTCTGAACAGATCATGAAGACAGGGGCCTTTTTGCTACAGGGTTTCATCCAGGATCGAGCAGGGAGGATGGCTGGGGAGACACCTGAGCTGACCTTGGAGCAGCCGCCCCAGGATGCGTCCACCAAGAAGCTGAGCGAGTGTCTCCGGCGAATTGGAGATGAACTGGATAGCAATATGGAGCTGCAGAGGATGATTGCTGACGTGGACACGGACTCCCCCCGAGAGGTCTTCTTCCGGGTGGCAGCTGACATGTTTGCTGATGGCAACTTCAACTGGGGCCGCGTGGTTGCCCTCTTCTACTTTGCTAGCAAACTGGTGCTCAAGGCCCTGTGCACTAAAGTGCCCGAGCTGATCAGAACCATCATGGGCTGGACACTGGACTTCCTCCGTGAGCGGCTGCTTGTCTGGATCCAAGACCAGGGTGGCTGGGAAGGCCTCCTCTCCTACTTCGGGACCCCCACATGGCAGACAGTGACCATCTTTGTGGCTGGAGTCCTCACCGCCTCGCTCACCATCTGGAAGAAGATGGGCTGA" 
COMMENT              "Fragment of pRS415GAL1mBAX; Length: 579" 0 (CStringList) 0) 
COMMENT         #78=(COligo "mob'" 
COMMENT              "ATGGCGGCATACGCGATCATGCGATGCAAGAAGCTGGCGAAAATGGGCAACGTGGCGGCCAGTCTCAAGCACGCCTACCGCGAGCGCGAGACGCCCAACGCTGACGCCAGCAGGACGCCAGAGAACGAGCACTGGGCGGCCAGCAGCACCGATGAAGCGATGGGCCGACTGCGCGAGTTGCTGCCAGAGAAGCGGCGCAAGGACGCTGTGTTGGCGGTCGAGTACGTCATGACGGCCAGCCCGGAATGGTGGAAGTCGGCCAGCCAAGAACAGCAGGCGGCGTTCTTCGAGAAGGCGCACAAGTGGCTGGCGGACAAGTACGGGGCGGATCGCATCGTGACGGCCAGCATCCACCGTGACGAAACCAGCCCGCACATGACCGCGTTCGTGGTGCCGCTGACGCAGGACGGCAGGCTGTCGGCCAAGGAGTTCATCGGCAACAAAGCGCAGATGACCCGCGACCAGACCACGTTTGCGGCCGCTGTGGCCGATCTAGGGCTGCAACGGGGCATCGAGGGCAGCAAGGCACGTCACACGCGCATTCAGGCGTTCTACGAGGCCCTGGAGCGGCCACCAGTGGGCCACGTCACCATCAGCCCGCAAGCGGTCGAGCCACGCGCCTATGCACCGCAGGGATTGGCCGAAAAGCTGGGAATCTCAAAGCGCGTTGAGACGCCGGAAGCCGTGGCCGACCGGCTGACAAAAGCGGTTCGGCAGGGGTATGAGCCTGCCCTACAGGCCGCCGCAGGAGCGCGTGAGATGCGCAAGAAGGCCGATCAAGCCCAAGAGACGGCCCGAGACCTTCGGGAGCGCCTGAAGCCCGTTCTGGACGCCCTGGGGCCGTTGAATCGGGATATGCAGGCCAAGGCCGCCGCGATCATCAAGGCCGTGGGCGAAAAGCTGCTGACGGAACAGCGGGAAGTCCAGCGCCAGAAACAGGCCCAGCGCCAGCAGGAACGCGGGCGCG" 
COMMENT              "incomplete but functional mobilization gene; 6 C-terminal codons missing" 
COMMENT              0 (CStringList) 0) 
COMMENT         #79=(COligo "Myc epitope" "GARCARAARYTNATHWSNGARGARGAYYTN" 
COMMENT              "Fragment of MYC-TAG; Length: 30" 0 
COMMENT              (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #80=(COligo "neomycin" 
COMMENT              "ATGATTGAACAAGATGGATTGCACGCAGGTTCTCCGGCCGCTTGGGTGGAGAGGCTATTCGGCTATGACTGGGCACAACAGACAATCGGCTGCTCTGATGCCGCCGTGTTCCGGCTGTCAGCGCAGGGGCGCCCGGTTCTTTTTGTCAAGACCGACCTGTCCGGTGCCCTGAATGAACTGCAGGACGAGGCAGCGCGGCTATCGTGGCTGGCCACGACGGGCGTTCCTTGCGCAGCTGTGCTCGACGTTGTCACTGAAGCGGGAAGGGACTGGCTGCTATTGGGCGAAGTGCCGGGGCAGGATCTCCTGTCATCTCACCTTGCTCCTGCCGAGAAAGTATCCATCATGGCTGATGCAATGCGGCGGCTGCATACGCTTGATCCGGCTACCTGCCCATTCGACCACCAAGCGAAACATCGCATCGAGCGAGCACGTACTCGGATGGAAGCCGGTCTTGTCGATCAGGATGATCTGGACGAAGAGCATCAGGGGCTCGCGCCAGCCGAACTGTTCGCCAGGCTCAAGGCGCGCATGCCCGACGGCGAGGATCTCGTCGTGACCCATGGCGATGCCTGCTTGCCGAATATCATGGTGGAAAATGGCCGCTTTTCTGGATTCATCGACTGTGGCCGGCTGGGTGTGGCGGACCGCTATCAGGACATAGCGTTGGCTACCCGTGATATTGCTGAAGAGCTTGGCGGCGAATGGGCTGACCGCTTCCTCGTGCTTTACGGTATCGCCGCTCCCGATTCGCAGCGCATCGCCTTCTATCGCCTTCTTGACGAGTTCTTCTGA" 
COMMENT              "Fragment of pCDNA-mCASP-8; Length: 795" 0 (CStringList) 0) 
COMMENT         #81=(COligo "nos promoter start" 
COMMENT              "GATCATGAGCGGAGAATTAAGGGAGTCACGTTATGACCCCCGCCGATGACGCGGGACAAGCCGTTTTACGTTTGGAACTGACAGAACCGCAACGATTGAA" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #82=(COligo "nos promoter stop" 
COMMENT              "AGGTCACTATCAGCTAGCAAATATTTCTTGTCAAAAATGCTCCACTGACGTTCCATAAATTCCCCTCGGTATCCAATTAGAGTCTCATATTCACTCTCAA" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #83=(COligo "ocs terminator start" 
COMMENT              "CTGCTTTAATGAGATATGCGAGACGCCTATGATCGCATGATATTTGCTTTCAATTCTGTTGTGCACGTTGTAAAAAACCTGAGCATGTGTAGCTCAGATC" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #84=(COligo "ocs terminator stop" 
COMMENT              "GTGGGCCCCCAAATGAAGTGCAGGTCAAACCTTGACAGTGACGACAAATCGTTGGGCGGGTCCAGGGCGAATTTTGCGACAACATGTCGAGGCTCAGCAG" 
COMMENT              "Fragment of pEXSOD10; Length: 100" 0 (CStringList) 0) 
COMMENT         #85=(COligo "p15A ori start" 
COMMENT              "GGAGCGCAGTCACCAAAACTTGTCCTTTCAGTTTAGCCTTAACCGGCGCATGACTTCAAGACTAACTCCTCTAAATCAATTACCAGTGGCTGCTGCCAGT" 
COMMENT              "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #86=(COligo "p15A ori stop" 
COMMENT              "GTCAGGGGGGCGGAGCCTATGGAAAAACGGCTTTGCCGCGGCCCTCTCACTTCCCTGTTAAGTATCTTCCTGGCATCTTCCAGGAAATCTCCGCCCCGTT" 
COMMENT              "Fragment of pACYC177; Length: 100" 0 (CStringList) 0) 
COMMENT         #87=(COligo "parA" 
COMMENT              "TTGAAGGTCGCCCGCATCTACCTGCGCGCCAGTACGGACGAGCAGAATCTTGAACGCCAGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTGGAGAGCCTTGTAGCGGCCACGCGGGCCGCCGGGTACTACGTCGCCGGCATCTACCGCGAGAAGGCGTCCGGCGCACGCGCCGACCGGCCCGAGCTGCTGCGCATGATCGCGGACCTGCAACCTGGTGAAGTCGTCGTTGCGGAGAAGATCGACCGCATCAGCCGCTTGCCGTTGGCCGAGGCCGAGCGCCTGGTTGCGTCGATCCGGGCCAAAGGGGCCAAGCTGGCCGTGCCTGGCGTGGTGGACCTGTCGGAGCTGGCCGCCGAGGCGAACGGAGTGGCGAAAATCGTTCTGGAATCCGTCCAGGACATGCTTTTGAAGCTCGCCTTGCAGATGGCCCGCGACGACTACGAGGATCGGCGCGAGCGTCAACGTCAGGGTGTCCAGTTGGCGAAGGCCGCCGGCCGCTACACCGGCCGCAAACGTGACGCCGGCATGCACGACCGCATCATCACGCTTCGCTCCGGCGGATCGAGCATTGCCAAGACGGCCAAGCTGGTCGGATGCAGCCCGAGCCAGGTCAAACGAGTGTGGGCGGCCTGGAACGCGCAGCAGCAAAAATAA" 
COMMENT              "Fragment of pRH001; Length: 762" 0 (CStringList) 0) 
COMMENT         #88=(COligo "parB" 
COMMENT              "ATGAAGCGCCGAAGCTACGCCATGCTGCGCGCCGCTGCCGCGCTGGCCGTCCTGGTCGTTGCCTCGCCGGCATGGGCCGAGCTGCGCGGCGAGGTCGTGCGCATCATCGACGGCGACACCATCGACGTGCTGGTAGACAAGCAGCCGGTGCGCGTGCGCCTGGTGGACATTGACGCGCCGGAAAAGCGGCAAGCCTTCGGCGAACGTGCGCGCCAGGCGCTGGCCGGCATGGTGTTCCGCCGGCACGTCCTGGTCGACGAGAAGGACACCGACCGTTACGGCCGCACGCTGGGCACCGTGTGGGTCAACATGGAGCTGGCCAGCCGGCCGCCGCAGCCGCGCAACGTCAACGCCGCGATGGTTCACCAGGGCATGGCGTGGGCCTATCGCTTCCACGGCCGCGCGGCCGACCCTGAAATGCTGCGGCTCGAACAGGAGGCGCGAGGCAAGCGCGTCGGCCTCTGGTCCGATCCGCACGCCGTCGAGCCGTGGAAATGGCGACGCGAGAGCAACAACCGGAGGGACGAAGGTTGA" 
COMMENT              "Fragment of pRH001; Length: 534" 0 (CStringList) 0) 
COMMENT         #89=(COligo "parC" 
COMMENT              "ATGGGTATACCCAATTTGACCAAGGGGGACGCGATGAGGGCGGCCAAGCACTACCGACAACTTCTATCCATCGACTTCAACATCGAGGCGCTGGCCTTCGTGCCTGGACCCGACGGCACACGCGGCCGGCGCATCCACGTCCTGGGGCGCGAGGTCCGCGACCGGCCCGGCCTGGTCGAGTACCTTTCGCCGGCGTTCGGCTCGCGGGTGGCGCTGGACGGCTACTGCAAGGCCAATTTCGATGCAGTGCTGCACCTGGCGTACCCCGATCATCAGCAATGGGGCCACGCATGA" 
COMMENT              "Fragment of pRH001; Length: 294" 0 (CStringList) 0) 
COMMENT         #90=(COligo "parD" 
COMMENT              "ATGAGCCGCCTGACAATCGACATGACGGACCAGCAGCACCAGAGCCTGAAAGCCCTGGCCGCCTTGCAGGGCAAGACCATTAAGCAATACGCCCTCGAACGTCTGTTCCCCGGTGACGCTGATGCCGATCAGGCATGGCAGGAACTGAAAACCATGCTGGGGAACCGCATCAACGATGGGCTTGCCGGCAAGGTGTCCACCAAGAGCGTCGGCGAAATTCTTGATGAAGAACTCAGCGGGGATCGCGCTTGA" 
COMMENT              "Fragment of pMR10cat; Length: 252" 0 (CStringList) 0) 
COMMENT         #91=(COligo "parE" 
COMMENT              "TTGACGGCCTACATCCTCACGGCTGAGGCCGAAGCCGATCTACGCGGCATCATCCGCTACACGCGCCGGGAGTGGGGCGCGGCGCAGGTGCGCCGCTATATCGCTAAGCTGGAACAGGGCATAGCCAGGCTTGCCGCCGGCGAAGGCCCGTTTAAGGACATGAGCGAACTCTTTCCCGCGCTGCGGATGGCCCGCTGCGAACACCACTACGTTTTTTGCCTGCCGCGTGCGGGCGAACCCGCGTTGGTCGTGGCGATCCTGCATGAGCGCATGGACCTCATGACGCGACTTGCCGACAGGCTCAAGGGCTGA" 
COMMENT              "Fragment of pMR10cat; Length: 312" 0 (CStringList) 0) 
COMMENT         #92=(COligo "pBBR1 ori" 
COMMENT              "ATGGCCACGCAGTCCAGAGAAATCGGCATTCAAGCCAAGAACAAGCCCGGTCACTGGGTGCAAACGGAACGCAAAGCGCATGAGGCGTGGGCCGGGCTTATTGCGAGGAAACCCACGGCGGCAATGCTGCTGCATCACCTCGTGGCGCAGATGGGCCACCAGAACGCCGTGGTGGTCAGCCAGAAGACACTTTCCAAGCTCATCGGACGTTCTTTGCGGACGGTCCAATACGCAGTCAAGGACTTGGTGGCCGAGCGCTGGATCTCCGTCGTGAAGCTCAACGGCCCCGGCACCGTGTCGGCCTACGTGGTCAATGACCGCGTGGCGTGGGGCCAGCCCCGCGACCAGTTGCGCCTGTCGGTGTTCAGTGCCGCCGTGGTGGTTGATCACGACGACCAGGACGAATCGCTGTTGGGGCATGGCGACCTGCGCCGCATCCCGACCCTGTATCCGGGCGAGCAGCAACTACCGACCGGCCCCGGCGAGGAGCCGCCCAGCCAGCCCGGCATTCCGGGCATGGAACCAGACCTGCCAGCCTTGACCGAAACGGAGGAATGGGAACGGCGCGGGCAGCAGCGCCTGCCGATGCCCGATGAGCCGTGTTTTCTGGACGATGGCGAGCCGTTGGAGCCGCCGACACGGGTCACGCTGCCGCGCCGGTAG" 
COMMENT              "" 0 (CStringList) 0) 
COMMENT         #93=(COligo "pColE1 ori" 
COMMENT              "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCTATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCTGCTACGTGGCCTTCTTCCTGTTCCTGGTCTTTTGCTCACATGTTCTTTCCGGCCTTATCCCCTGATTCTGTGGATAACTGTGTTACCGTTTTTGTGTGAGTCAGTACCGCTCGCCGCAGTCGAACGACCGAGCGTAGCGAGTCAGTGAGCGAGGAAGCGGAAAAGC" 
COMMENT              "Fragment of pPLa2311trpA1; Length: 392" 0 (CStringList) 0) 
COMMENT         #94=(COligo "PL promoter" 
COMMENT              "TCTCTCACCTACCAAACAATGCCCCCCTGCAAAAAATAAATTCATATAAAAAACATACAGATAACCATCTGCGGTGATAAATTATCTCTGGCGGTGTTGACATAAATACCACTGGCGGTGATACTGAGCAC" 
COMMENT              "Fragment of pLT-mCASP-1p30-1; Length: 131" 0 (CStringList) 0) 
COMMENT         #95=(COligo "pMB1 ori" 
COMMENT              "CTGAGATACCTACAGCGTGAGCTATGAGAAAGCGCCACGCTTCCCGAAGGGAGAAAGGCGGACAGGTATCCGGTAAGCGGCAGGGTCGGAACAGGAGAGCGCACGAGGGAGCTTCCAGGGGGAAACGCCTGGTATCTTTATAGTCCTGTCGGGTTTCGCCACCTCTGACTTGAGCGTCGATTTTTGTGATGCTCGTCAGGGGGGCGGAGCCTATGGAAAAACGCCAGCAACGCGGCCTTTTTACGGTTCCTGGCCTTTTGCTGGCCTTTTGCTCACATGTTCTTTCCTGCGTTATCCCCTGATTCTGTGGATAACCGTATTACCGCCTTTGAGTGAGCTGATACCGCTCGCCGCAGCCGAACGACCGAGCGCAGCGAGTCAGTGAGCGAGGAAGCGGAAGAGC" 
COMMENT              "Fragment of pRH007; Length: 403" 0 (CStringList) 0) 
COMMENT         #96=(COligo "polyoma ori" 
COMMENT              "CTGTTTTTTTTAGTATTAAGCAGAGGCCGGGGACCCCTGGGCCCGCTTACTCTGGAGAAAAAGAAGAGAGGCATTGTAGAGGCTTCCAGAGGCAACTTGTCAAAACAGGACTG" 
COMMENT              "Fragment of pCDM92; Length: 113" 0 (CStringList) 0) 
COMMENT         #97=(COligo "ppMF" 
COMMENT              "ATGAGATTTCCTTCAATTTTTACTGCTGTTTTATTCGCAGCATCCTCCGCATTAGCTGCTCCAGTCAACACTACAACAGAAGATGAAACGGCACAAATTCCGGCTGAAGCTGTCATCGGTTACTCAGATTTAGAAGGGGATTTCGATGTTGCTGTTTTGCCATTTTCCAACAGCACAAATAACGGGTTATTGTTTATAAATACTACTATTGCCAGCATTGCTGCTAAAGAAGAAGGGGTATCT" 
COMMENT              "Fragment of pGAPZMFManHDEL; Length: 243" 0 (CStringList) 0) 
COMMENT         #98=(COligo "R6K ori" 
COMMENT              "TCTGAAGATCAGCAGTTCAACCTGTTGATAGTACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTAACGTACTAAGCTCTCATGTTTAACGAACTAAACCCTCATGGCTAACGTACTAAGCTCTCATGGCTAACGTACTAAGCTCTCATGTTTCACGTACTAAGCTCTCATGTTTGAACAATAAAATTAATATAAATCAGCAACTTAAATAGCCTCTAAGGTTTTAAGTTTTATAAGAAAAAAAAGAATATATAAGGCTTTTAAAGCTTTTAAGGTTTAACGGTTGTGGACAACAAGCCAGGGATGTAACGCACTGAGAAGCCCTTAGAGCCTCTCAAAGCAATTTTCAGTGACACAGGAACACTTAACGGCTGACAT" 
COMMENT              "Fragment of pKNG101; Length: 388" 0 (CStringList) 0) 
COMMENT         #99=(COligo "RK2 ori" 
COMMENT              "GGCCGCCGGCGTTGTGGATACCTCGCGGAAAACTTGGCCCTCACTGACAGATGAGGGGCGGACGTTGACACTTGAGGGGCCGACTCACCCGGCGCGGCGTTGACAGATGAGGGGCAGGCTCGATTTCGGCCGGCGACGTGGAGCTGGCCAGCCTCGCAAATCGGCGAAAACGCCTGATTTTACGCGAGTTTCCCACAGATGATGTGGACAAGCCTGGGGATAAGTGCCCTGCGGTATTGACACTTGAGGGGCGCGACTACTGACAGATGAGGGGCGCGATCCTTGACACTTGAGGGGCAGAGTGCTGACAGATGAGGGGCGCACCTATTGACATTTGAGGGGCTGTCCACAGGCAGAAAATCCAGCATTTGCAAGGGTTTCCGCCCGTTTTTCGGCCACCGCTAACCTGTCTTTTAACCTGCTTTTAAACCAATATTTATAAACCTTGTTTTTAACCAGGGCTGCGCCCTGTGCGCGTGACCGCGCACGCCGAAGGGGGGTGCCCCCCCTTCTCGAACCCTCCCGG" 
COMMENT              "Fragment of pRH001; Length: 526" 0 (CStringList) 0) 
COMMENT         #100=(COligo "RK2/RP4 oriT" 
COMMENT               "CGGCCAGCCTCGCAGAGCAGGATTCCCGTTGAGCACCGCCAGGTGCGAATAAGGGACAGTGAAGAAGGAACACCCGCTCGCGGGTGGGCCTACTTCACCTATCCTGCCCGG" 
COMMENT               "Fragment of pRH001; Length: 111" 0 (CStringList) 0) 
COMMENT         #101=(COligo "rop" 
COMMENT               "GTGACCAAACAGGAAAAAACCGCCCTTAACATGGCCCGCTTTATCAGAAGCCAGACATTAACGCTTCTGGAGAAACTCAACGAGCTGGACGCGGATGAACAGGCAGACATCTGTGAATCGCTTCACGACCACGCTGATGAGCTTTACCGCAGCTGCCTCGCGCGTTTCGGTGATGACGGTGAAAACCTCTGA" 
COMMENT               "Fragment of pBR322; Length: 192" 0 (CStringList) 0) 
COMMENT         #102=(COligo "SP6 promoter" "ATTTAGGTGACACTATA" 
COMMENT               "Fragment of pdCS2-MT ; Length: 17" 0 (CStringList) 0) 
COMMENT         #103=(COligo "Strep-tag" "GCTTGGCGTCACCCGCAGTTCGGTGGT" 
COMMENT               "pUChGNTI2: 27 nucleotides" 0 (CStringList) 0) 
COMMENT         #104=(COligo "supF" 
COMMENT               "TCTTTCTCAACGTAACACTTTACAGCGGCGCGTCATTTGATATGATGCGCCCCGCTTCCCGATAAGGGAGCAGGCCAGTAAAAGCATTACCCGTGGTGGGGTTCCCGAGCGGCCAAAGGGAGCAGACTCTAAATCTGCCGTCATCGACTTCGAAGGTTCGAATCCTTCCCCCACCACCATCACTTTCAAAAGTCCGAAAGAAT" 
COMMENT               "Fragment of pCDM92; Length: 203" 0 (CStringList) 0) 
COMMENT         #105=(COligo "SV40 early promoter" 
COMMENT               "CTGTGGAATGTGTGTCAGTTAGGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCAGGTGTGGAAAGTCCCCAGGCTCCCCAGCAGGCAGAAGTATGCAAAGCATGCATCTCAATTAGTCAGCAACCATAGTCCCGCCCCTAACTCCGCCCATCCCGCCCCTAACTCCGCCCAGTTCCGCCCATTCTCCGCCCCATGGCTGACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCTGCC" 
COMMENT               "Fragment of pEF6/Myc-HisA; Length: 282" 0 (CStringList) 0) 
COMMENT         #106=(COligo "SV40 ori" 
COMMENT               "GACTAATTTTTTTTATTTATGCAGAGGCCGAGGCCGCCTCKGCCTCTGAGCTATTCCAGAAGTAGTGAGGAGGCTT" 
COMMENT               "Fragment of SV40; Length: 76" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #107=(COligo "SV40 polyA early" 
COMMENT               "AATGAATGCAATTGTTGTTGTTAACTTGTTTATTGCAGCTTATAATGGTTACAAATAAAGCAATAGCATCACAAATTTCACAAATAAAGCATTTTTT" 
COMMENT               "Fragment of pdEYFP; Length: 97" 0 (CStringList) 0) 
COMMENT         #108=(COligo "T1 terminator" 
COMMENT               "ATAAAACGAAAGGCCCAGTCTTCCGACTGAGCCTTTCGTTTTAT" 
COMMENT               "Fragment of pDONR201; Length: 44" 0 (CStringList) 0) 
COMMENT         #109=(COligo "T2 terminator" "AGAAGGCCATCCTGACGGATGGCCTTTT" 
COMMENT               "Fragment of pDONR201; Length: 28" 0 (CStringList) 0) 
COMMENT         #110=(COligo "T3 promoter" "AATTAACCCTCACTAAA" 
COMMENT               "Fragment of pLT-mCASP-14" 0 (CStringList) 0) 
COMMENT         #111=(COligo "T7 promoter" "TAATACGACTCACTATA" 
COMMENT               "Fragment of pGEM-TAX1BP1-S; Length: 17" 0 (CStringList) 0) 
COMMENT         #112=(COligo "T7 RBS" 
COMMENT               "GGGAGACCACAACGGTTTCCCTCTAGAAATAATTTTGTTTAACTTTAAGAAGGAGATATACAT" 
COMMENT               "Fragment of pLT10Bgal; Length: 63" 0 (CStringList) 0) 
COMMENT         #113=(COligo "T7 terminator" 
COMMENT               "CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG" 
COMMENT               "Fragment of pGBKT7; Length: 48" 0 (CStringList) 0) 
COMMENT         #114=(COligo "tac promoter" "GTTGACAATTAATCATCGGCTCGTATAATGTGTGG" 
COMMENT               "Fragment of pGEX5X1; Length: 35" 0 (CStringList) 0) 
COMMENT         #115=(COligo "TEF1 E. gossypii" 
COMMENT               "GGTTGTTTATGTTCGGATGTGATGTGAGAACTGTATCCTAGCAAGATTTTAAAAGGAAGTATATGAAAGAAGAACCTCAGTGGCAAATCCTAACCTTTTATATTTCTCTACAGGGGCGCGGCGTGGGGACAATTCAACGCGTCTGTGAGGGGAGCGTTTCCCTGCTCGCAGGTCTGCAGCGAGGAGCCGTAATTTTTGCTTCGCGCCGTGCGGCCATCAAAATGTATGGATGCAAATGATTATACATGGGGATGTATGGGCTAAATGTACGGGCGACAGTCACATCATGCCCCTGAGCTGCGCACGTCAAGACTGTCAAGGAGGGTATTCTGGGCCTCCATGTC" 
COMMENT               "E. gossypii TEF1alpha promoter" 0 (CStringList) 0) 
COMMENT         #116=(COligo "TEF1 terminator start" 
COMMENT               "AGTAACTGACAATAAAAAGATTCTTGTTTTCAAGAACTTGTCATTTGTATAGTTTTTTTATATTGTAGTTGTTCTATTTTAATCAAATGTTAGCGTGATT" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #117=(COligo "TEF1 terminator stop" 
COMMENT               "ATATTTTTTTTGCCTCGACATCATCTGCCCAGATGCGAAGTTAAGTGCGCAGAAAGTAATATCATGCGTCAATCGTATGTGAATGCTGGTCGCTATACTG" 
COMMENT               "Fragment of Eremothecium gossypii TEF1; Length: 100" 0 
COMMENT               (CStringList "nagekeken 06/2005 AO") 0) 
COMMENT         #118=(COligo "TEF1a promoter start" 
COMMENT               "CACCATAGCTTCAAAATGTTTCTACTCCTTTTTTACTCTTCCAGATTTTCTCGGACTCCGCGCATCGCCGTACCACTTCAAAACACCCAAGCACAGCATA" 
COMMENT               "Fragment of pFLD; Length: 100" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB") 0) 
COMMENT         #119=(COligo "TEF1a promoter stop" 
COMMENT               "GGTCTTCAATTTCTCAAGTTTCAGTTTCATTTTTCTTGTTCTATTACAACTTTTTTTACTTCTTGYTCATTAGAAAGAAAGCATAGCAATCTAATCTAAG" 
COMMENT               "Fragment of pFLD; Length: 100" 0 
COMMENT               (CStringList "nagekeken 09/2004 MB" "nagekeken 06/2005 AO") 0) 
COMMENT         #120=(COligo "TET start" 
COMMENT               "ATGAAATCTAACAATGCGCTCATCGTCATCCTCGGCACCGTCACCCTGGATGCTGTAGGCATAGGCTTGGTTATGCCGGTACTGCCGGGCCTCTTGCGGG" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #121=(COligo "TET stop" 
COMMENT               "CACATGGAACGGGTTGGCATGGATTGTAGGCGCCGCCCTATACCTTGTCTGCCTCCCCGCGTTGCGTCGCGGTGCATGGAGCCGGGCCACCTCGACCTGA" 
COMMENT               "Fragment of pBR322; Length: 100" 0 (CStringList) 0) 
COMMENT         #122=(COligo "thrombin recognition site" "CTGGTTCCGCGTGGATCC" 
COMMENT               "Fragment of pGEX-4T2; Length: 18" 0 (CStringList) 0) 
COMMENT         #123=(COligo "TRP1" 
COMMENT               "ATGTCTGTTATTAATTTCACAGGTAGTTCTGGTCCATTGGTGAAAGTTTGCGGCTTGCAGAGCACAGAGGCCGCAGAATGTGCTCTAGATTCCGATGCTGACTTGCTGGGTATTATATGTGTGCCCAATAGAAAGAGAACAATTGACCCGGTTATTGCAAGGAAAATTTCAAGTCTTGTAAAAGCATATAAAAATAGTTCAGGCACTCCGAAATACTTGGTTGGCGTGTTTCGTAATCAACCTAAGGAGGATGTTTTGGCTCTGGTCAATGATTACGGCATTGATATCGTCCAACTGCATGGAGATGAGTCGTGGCAAGAATACCAAGAGTTCCTCGGTTTGCCAGTTATTAAAAGACTCGTATTTCCAAAAGACTGCAACATACTACTCAGTGCAGCTTCACAGAAACCTCATTCGTTTATTCCCTTGTTTGATTCAGAAGCAGGTGGGACAGGTGAACTTTTGGATTGGAACTCGATTTCTGACTGGGTTGGAAGGCAAGAGAGCCCCGAAAGCTTACATTTTATGTTAGCTGGTGGACTGACGCCAGAAAATGTTGGTGATGCGCTTAGATTAAATGGCGTTATTGGTGTTGATGTAAGCGGAGGTGTGGAGACAAATGGTGTAAAAGACTCTAACAAAATAGCAAATTTCGTCAAAAATGCTAAGAAATAG" 
COMMENT               "Fragment of pAS2; Length: 675" 0 (CStringList) 0) 
COMMENT         #124=(COligo "URA3" 
COMMENT               "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT               "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0) 
COMMENT         #125=(COligo "V5 epitope" "GGNAARCCNATHCCNAAYCCNYTNYTNGGNYTNGAYWSNACN" 
COMMENT               "length: 42 bp" 0 (CStringList) 0) 
COMMENT         #126=(COligo "VSV epitope" "TACACCGACATCGAGATGAACCGGTTGGGCAAG" 
COMMENT               "Fragment of pCR3VSV-CED-4; Length: 33" 0 (CStringList) 0) 
COMMENT         #127=(COligo "XGPRT" 
COMMENT               "ATGAGCGAAAAATACATCGTCACCTGGGACATGTTGCAGATCCATGCACGTAAACTCGCAAGCCGACTGATGCCTTCTGAACAATGGAAAGGCATTATTGCCGTAAGCCGTGGCGGTCTGGTACCGGGTGCGTTACTGGCGCGTGAACTGGGTATTCGTCATGTCGATACCGTTTGTATTTCCAGCTACGATCACGACAACCAGCGCGAGCTTAAAGTGCTGAAACGCGCAGAAGGCGATGGCGAAGGCTTCATCGTTATTGATGACCTGGTGGATACCGGTGGTACTGCGGTTGCGATTCGTGAAATGTATCCAAAAGCGCACTTTGTCACCATCTTCGCAAAACCGGCTGGTCGTCCGCTGGTTGATGACTATGTTGTTGATATCCCGCAAGATACCTGGATTGAACAGCCGTGGGATATGGGCGTCGTATTCGTCCCGCCAATCTCCGGTCGCTAA" 
COMMENT               "Fragment of pDelta1; Length: 459" 0 (CStringList) 0) 
COMMENT         #128=(COligo "Xpress epitope" "GAYYTNTAYGAYGAYGAYGAYAAR" 
COMMENT               "Fragment of pYD1TSE ; Length: 24" 0 (CStringList) 0) 
COMMENT         #129=(COligo "zeocin" 
COMMENT               "ATGGCCAAGTTGACCAGTGCCGTTCCGGTGCTCACCGCGCGCGACGTCGCCGGAGCGGTCGAGTTCTGGACCGACCGGCTCGGGTTCTCCCGGGACTTCGTGGAGGACGACTTCGCCGGTGTGGTCCGGGACGACGTGACCCTGTTCATCAGCGCGGTCCAGGACCAGGTGGTGCCGGACAACACCCTGGCCTGGGTGTGGGTGCGCGGCCTGGACGAGCTGTACGCCGAGTGGTCGGAGGTCGTGTCCACGAACTTCCGGGACGCCTCCGGGCCGGCCATGACCGAGATCGGCGAGCAGCCGTGGGGGCGGGAGTTCGCCCTGCGCGACCCGGCCGGCAACTGCGTGCACTTCGTGGCCGAGGAGCAGGACTGA" 
COMMENT               "Fragment of pGAPZMFManHDEL; Length: 375" 0 (CStringList) 0) 
COMMENT         #130=(COligo "\337-globin polyA" 
COMMENT               "GATCTTTTTCCCTCTGCCAAAAATTATGGGGACATCATGAAGCCCCTTGAGCATCTGACTTCTGGSTAATAAAGGAAATTTATTTTCATTGC" 
COMMENT               "Fragment of pBI-G; Length: 92" 0 (CStringList) 0)) 1 "{(0,1),2}" 
COMMENT        0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 4294967295 1 7483 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #131=(CRSite (CStringList) "EcoRI" "gaattc" 2 0 1 2763 0 0 "") 
COMMENT        #132=(CRSite (CStringList) "HindII" "gtyrac" 4 0 4 123 0 357 0 2652 0 
COMMENT              5429 0 0 "") 
COMMENT        #133=(CRSite (CStringList) "KpnI" "ggtacc" 6 0 1 1718 0 0 "") 
COMMENT        #134=(CRSite (CStringList) "NaeI" "gccggc" 4 0 5 1176 0 2637 0 3609 0 
COMMENT              4950 0 5235 0 0 "") 
COMMENT        #135=(CRSite (CStringList) "PstI" "ctgcag" 6 0 4 790 0 1295 0 2519 0 
COMMENT              4501 0 0 "") 
COMMENT        #136=(CRSite (CStringList) "SspI" "aatatt" 4 0 1 4 0 0 "") 
COMMENT        #137=(CRSite (CStringList) "XbaI" "tctaga" 2 0 1 3097 0 0 "") 
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FEATURES             Location/Qualifiers
     promoter        508..1651
                     /vntifkey="29"
                     /label=hEF1\promoter
     promoter        1668..1684
                     /vntifkey="30"
                     /label=T7\promoter
     intron          704..1642
                     /vntifkey="15"
                     /label=intron\hEF1\promoter
     misc_marker     3114..3155
                     /vntifkey="22"
                     /label=V5\epitope
     misc_marker     3165..3182
                     /vntifkey="22"
                     /label=His-tag
     terminator      3211..3435
                     /vntifkey="43"
                     /label=bovine\growth\hormone\polyA
     rep_origin      3481..3909
                     /vntifkey="33"
                     /label=f1\ori
     promoter        3914..4195
                     /vntifkey="29"
                     /label=SV40\early\promoter
     rep_origin      4152..4227
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      5289..5363
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5289..5363)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     CDS             complement(6609..7469)
                     /vntifkey="4"
                     /label=ampicillin
     CDS             4319..5113
                     /vntifkey="4"
                     /label=neomycin
                     /note="Tn5 antibiotic resistance gene; coding sequence"
     rep_origin      complement(5677..6068)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1719..3095
                     /vntifkey="4"
                     /label=hOMI\mutant
     promoter        complement(5502..5585)
                     /vntifkey="30"
                     /label=lac\promoter
     mutation        2634..2639
                     /vntifkey="62"
                     /label=S306A
                     /note="WT: TCT.GGA"
BASE COUNT     1569 a      1983 c      2094 g      1837 t 
ORIGIN
        1 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 
       61 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 
      121 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 
      181 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 
      241 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 
      301 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 
      361 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 
      421 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 
      481 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 
      541 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 
      601 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 
      661 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 
      721 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 
      781 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 
      841 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 
      901 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 
      961 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 
     1021 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 
     1081 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 
     1141 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 
     1201 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 
     1261 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 
     1321 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 
     1381 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 
     1441 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 
     1501 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 
     1561 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 
     1621 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 
     1681 tatagggaga cccaagctgg ctaggtaagc ttggtaccat ggctgcgccg agggcggggc 
     1741 ggggtgcagg ctggagcctt cgggcatggc gggctttggg gggcattcgc tgggggagga 
     1801 gaccccgttt gacccctgac ctccgggccc tgctgacgtc aggaacttct gacccccggg 
     1861 cccgagtgac ttatgggacc cccagtctct gggcccggtt gtctgttggg gtcactgaac 
     1921 cccgagcatg cctgacgtct gggaccccgg gtccccgggc acaactgact gcggtgaccc 
     1981 cagataccag gacccgggag gcctcagaga actctggaac ccgttcgcgc gcgtggctgg 
     2041 cggtggcgct gggcgctggg ggggcagtgc tgttgttgtt gtggggcggg ggtcggggtc 
     2101 ctccggccgt cctcgccgcc gtccctagcc cgccgcccgc ttctccccgg agtcagtaca 
     2161 acttcatcgc agatgtggtg gagaagacag cacctgccgt ggtctatatc gagatcctgg 
     2221 accggcaccc tttcttgggc cgcgaggtcc ctatctcgaa cggctcagga ttcgtggtgg 
     2281 ctgccgatgg gctcattgtc accaacgccc atgtggtggc tgatcggcgc agagtccgtg 
     2341 tgagactgct aagcggcgac acgtatgagg ccgtggtcac agctgtggat cccgtggcag 
     2401 acatcgcaac gctgaggatt cagactaagg agcctctccc cacgctgcct ctgggacgct 
     2461 cagctgatgt ccggcaaggg gagtttgttg ttgccatggg aagtcccttt gcactgcaga 
     2521 acacgatcac atccggcatt gttagctctg ctcagcgtcc agccagagac ctgggactcc 
     2581 cccaaaccaa tgtggaatac attcaaactg atgcagctat tgattttgga aacgccggcg 
     2641 gtcccctggt taacctggat ggggaggtga ttggagtgaa caccatgaag gtcacagctg 
     2701 gaatctcctt tgccatccct tctgatcgtc ttcgagagtt tctgcatcgt ggggaaaaga 
     2761 agaattcctc ctccggaatc agtgggtccc agcggcgcta cattggggtg atgatgctga 
     2821 ccctgagtcc cagcatcctt gctgaactac agcttcgaga accaagcttt cccgatgttc 
     2881 agcatggtgt actcatccat aaagtcatcc tgggctcccc tgcacaccgg gctggtctgc 
     2941 ggcctggtga tgtgattttg gccattgggg agcagatggt acaaaatgct gaagatgttt 
     3001 atgaagctgt tcgaacccaa tcccagttgg cagtgcagat ccggcgggga cgagaaacac 
     3061 tgaccttata tgtgacccct gaggtcacag aatgatctag agggcccttc gaaggtaagc 
     3121 ctatccctaa ccctctcctc ggtctcgatt ctacgcgtac cggtcatcat caccatcacc 
     3181 attgagttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     3241 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     3301 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     3361 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     3421 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     3481 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     3541 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     3601 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 
     3661 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     3721 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     3781 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     3841 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     3901 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     3961 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     4021 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     4081 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     4141 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     4201 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     4261 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 
     4321 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     4381 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     4441 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     4501 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     4561 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     4621 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     4681 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     4741 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     4801 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
     4861 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     4921 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     4981 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     5041 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     5101 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 
     5161 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
     5221 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
     5281 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     5341 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     5401 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 
     5461 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     5521 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     5581 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     5641 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     5701 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     5761 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagg 
     5821 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat 
     5881 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag 
     5941 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga 
     6001 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg 
     6061 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt 
     6121 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac 
     6181 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc 
     6241 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt 
     6301 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc 
     6361 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc 
     6421 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg 
     6481 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag 
     6541 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg 
     6601 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt 
     6661 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca 
     6721 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca 
     6781 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc 
     6841 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt 
     6901 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg 
     6961 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc 
     7021 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg 
     7081 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga 
     7141 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga 
     7201 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta 
     7261 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg 
     7321 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact 
     7381 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata 
     7441 agggcgacac ggaaatgttg aatactcata ctcttccttt ttc 
//
