ID pEF1-AVPS-hOMI standard; circular DNA; ; 7087 BP. DE LMBP 4582. CC Accession number: LMBP 4582 CC (c) BCCM/GeneCorner Plasmid collection (Ghent University, Belgium) CC Last modified: 11-JAN-2008 CC CC VNTAUTHORNAME|BCCM/GeneCorner Plasmid Collection| CC VNTAUTHORTEL|0032-9-33-13-843| CC VNTAUTHOREML|bccm.genecorner@UGent.be| CC VNTAUTHORWWW|www.genecorner.ugent.be| CC VNTAUTHORAD1|Ghent University| CC VNTAUTHORAD2|Technologiepark 927| CC VNTAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium| CC CC VNTREPLTYPE|Plasmid FH Key Location/Qualifiers FH FT promoter 508..1651 FT /vntifkey="29" FT /label=hEF1\promoter FT promoter 1668..1684 FT /vntifkey="30" FT /label=T7\promoter FT intron 704..1642 FT /vntifkey="15" FT /label=intron\hEF1\promoter FT misc_marker 2718..2759 FT /vntifkey="22" FT /label=V5\epitope FT misc_marker 2769..2786 FT /vntifkey="22" FT /label=His-tag FT terminator 2815..3039 FT /vntifkey="43" FT /label=bovine\growth\hormone\polyA FT rep_origin 3085..3513 FT /vntifkey="33" FT /label=f1\ori FT promoter 3518..3799 FT /vntifkey="29" FT /label=SV40\early\promoter FT rep_origin 3756..3831 FT /vntifkey="33" FT /label=SV40\ori FT terminator 4893..4967 FT /vntifkey="43" FT /label=SV40\polyA\early FT terminator complement(4893..4967) FT /vntifkey="43" FT /label=SV40\polyA\late FT CDS complement(6213..7073) FT /vntifkey="4" FT /label=ampicillin FT CDS 3923..4717 FT /vntifkey="4" FT /label=neomycin FT /note="Tn5 antibiotic resistance gene; coding sequence" FT rep_origin complement(5281..5672) FT /vntifkey="33" FT /label=pMB1\ori FT CDS 1719..2699 FT /vntifkey="4" FT /label=mature\hOMI FT promoter complement(5106..5189) FT /vntifkey="30" FT /label=lac\promoter SQ Sequence 7087 BP; 1525 A; 1858 C; 1936 G; 1768 t; aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta 60 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg 120 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga 180 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg 240 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct 300 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca 360 ttgattattg actaggcttt tgcaaaaagc tttgcaaaga tggataaagt tttaaacaga 420 gaggaatctt tgcagctaat ggaccttcta ggtcttgaaa ggagtgggaa ttggctccgg 480 tgcccgtcag tgggcagagc gcacatcgcc cacagtcccc gagaagttgg ggggaggggt 540 cggcaattga accggtgcct agagaaggtg gcgcggggta aactgggaaa gtgatgtcgt 600 gtactggctc cgcctttttc ccgagggtgg gggagaaccg tatataagtg cagtagtcgc 660 cgtgaacgtt ctttttcgca acgggtttgc cgccagaaca caggtaagtg ccgtgtgtgg 720 ttcccgcggg cctggcctct ttacgggtta tggcccttgc gtgccttgaa ttacttccac 780 ctggctgcag tacgtgattc ttgatcccga gcttcgggtt ggaagtgggt gggagagttc 840 gaggccttgc gcttaaggag ccccttcgcc tcgtgcttga gttgaggcct ggcctgggcg 900 ctggggccgc cgcgtgcgaa tctggtggca ccttcgcgcc tgtctcgctg ctttcgataa 960 gtctctagcc atttaaaatt tttgatgacc tgctgcgacg ctttttttct ggcaagatag 1020 tcttgtaaat gcgggccaag atctgcacac tggtatttcg gtttttgggg ccgcgggcgg 1080 cgacggggcc cgtgcgtccc agcgcacatg ttcggcgagg cggggcctgc gagcgcggcc 1140 accgagaatc ggacgggggt agtctcaagc tggccggcct gctctggtgc ctggcctcgc 1200 gccgccgtgt atcgccccgc cctgggcggc aaggctggcc cggtcggcac cagttgcgtg 1260 agcggaaaga tggccgcttc ccggccctgc tgcagggagc tcaaaatgga ggacgcggcg 1320 ctcgggagag cgggcgggtg agtcacccac acaaaggaaa agggcctttc cgtcctcagc 1380 cgtcgcttca tgtgactcca cggagtaccg ggcgccgtcc aggcacctcg attagttctc 1440 gagcttttgg agtacgtcgt ctttaggttg gggggagggg ttttatgcga tggagtttcc 1500 ccacactgag tgggtggaga ctgaagttag gccagcttgg cacttgatgt aattctcctt 1560 ggaatttgcc ctttttgagt ttggatcttg gttcattctc aagcctcaga cagtggttca 1620 aagttttttt cttccatttc aggtgtcgtg aggaattagc ttggtactaa tacgactcac 1680 tatagggaga cccaagctgg ctaggtaagc ttggtaccat ggccgtccct agcccgccgc 1740 ccgcttctcc ccggagtcag tacaacttca tcgcagatgt ggtggagaag acagcacctg 1800 ccgtggtcta tatcgagatc ctggaccggc accctttctt gggccgcgag gtccctatct 1860 cgaacggctc aggattcgtg gtggctgccg atgggctcat tgtcaccaac gcccatgtgg 1920 tggctgatcg gcgcagagtc cgtgtgagac tgctaagcgg cgacacgtat gaggccgtgg 1980 tcacagctgt ggatcccgtg gcagacatcg caacgctgag gattcagact aaggagcctc 2040 tccccacgct gcctctggga cgctcagctg atgtccggca aggggagttt gttgttgcca 2100 tgggaagtcc ctttgcactg cagaacacga tcacatccgg cattgttagc tctgctcagc 2160 gtccagccag agacctggga ctcccccaaa ccaatgtgga atacattcaa actgatgcag 2220 ctattgattt tggaaactct ggaggtcccc tggttaacct ggatggggag gtgattggag 2280 tgaacaccat gaaggtcaca gctggaatct cctttgccat cccttctgat cgtcttcgag 2340 agtttctgca tcgtggggaa aagaagaatt cctcctccgg aatcagtggg tcccagcggc 2400 gctacattgg ggtgatgatg ctgaccctga gtcccagcat ccttgctgaa ctacagcttc 2460 gagaaccaag ctttcccgat gttcagcatg gtgtactcat ccataaagtc atcctgggct 2520 cccctgcaca ccgggctggt ctgcggcctg gtgatgtgat tttggccatt ggggagcaga 2580 tggtacaaaa tgctgaagat gtttatgaag ctgttcgaac ccaatcccag ttggcagtgc 2640 agatccggcg gggacgagaa acactgacct tatatgtgac ccctgaggtc acagaatgat 2700 ctagagggcc cttcgaaggt aagcctatcc ctaaccctct cctcggtctc gattctacgc 2760 gtaccggtca tcatcaccat caccattgag tttaaacccg ctgatcagcc tcgactgtgc 2820 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag 2880 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta 2940 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag 3000 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca 3060 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg 3120 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg 3180 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg 3240 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt 3300 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt 3360 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta 3420 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa 3480 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg 3540 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta 3600 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat 3660 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac 3720 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga 3780 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg 3840 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcaaga 3900 gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag gttctccggc 3960 cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga 4020 tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct 4080 gtccggtgcc ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac 4140 gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct 4200 attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt 4260 atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt 4320 cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt 4380 cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag 4440 gctcaaggcg cgcatgcccg acggcgagga tctcgtcgtg acccatggcg atgcctgctt 4500 gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg 4560 tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg 4620 cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg 4680 catcgccttc tatcgccttc ttgacgagtt cttctgagcg ggactctggg gttcgcgaaa 4740 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct 4800 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg 4860 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt 4920 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta 4980 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta 5040 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca 5100 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag 5160 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 5220 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 5280 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 5340 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 5400 agaacatgtg agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 5460 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 5520 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 5580 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 5640 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 5700 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 5760 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 5820 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 5880 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 5940 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 6000 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 6060 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 6120 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 6180 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 6240 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 6300 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 6360 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 6420 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 6480 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 6540 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 6600 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 6660 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 6720 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 6780 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 6840 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 6900 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 6960 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 7020 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 7080 ctttttc 7087 //